• Journal of Animal Science and Technology
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• v.46 no.2
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• pp.155-164
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• 2004

This involves identifying and cloning trapped genes from cultured cells carrying the gene-trap constructs and generating cloned zebrafish using these cells for functional study. Gene-trapping studies in gene-trapped cells were carried out in initial and cloned zebrafish carrying gene-trap events were successfully produced based on the nuclear transplantation technique. Two kind of retroviral gene-trap constructs were adopted. The first one(SA/GFP-TP), constructed in my laboratory, carries a GFP reporter gene containing a splicing acceptor and an internal neo gene. The second one(Neo-TP), obtained from Dr. Hicks (Hicks et al., 1997), contains a promoter-less neo gene located in the LTR sequence of a retroviral vector. The infected cells were subjected to drug selection(neomycin treatment) because the two constructs carry the neomycin resistant gene. All those cells survived the neomycin treatment should carry the proviral insertions. For Neo-TP, Isolated DNA from the neomycin-resistant fibroblast cells infected by Neo-TP, was digested with EcoR1 restriction enzyme and transformed into bacteria after ligation. This procedure led to the isolation of seven clones carrying flanking cellular DNA with a typical retroviral integration signature sequence. These clones contained genomic DNA ranging from 1kb to 7kb and sequences of 300-600 bp were obtained from each of the rescued plasmids. Database searching showed that all of them share high homology to zebrafish sequences. For fish cloning using tagged cells, initially, nucleus donors directly selected from a mixture of cells(Neo-TP cells) were used. A total of 44 embryos(3.7%) out of 1179 transplants were reached blastula stage; 8 of these embryos(0.8%) hatched and 3(0.3%) of them survived to adulthood. One out of three lived cloned zebrafish has an amplified fragment and was labeled with 32P.

• Journal of Family Relations
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• v.22 no.3
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• pp.155-183
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• 2017

Objectives: The purpose of this study identified the perceived quality of parent-child relationships and attitudes toward filial duty contracts among young adult children. Also, this study is to examine the effects of sociodemographic characteristics and perceived quality parent-child relationships on the attitudes toward filial duty contracts and the requirement for filial duty contract details. Method: For this study, a survey was conducted with 210 college student children in young adulthood in Seoul and Gyeonggi-do. The questionnaire consisted of the 'Attitudes toward Filial Duty Contracts Scale', the 'Requirement for Filial Duty Contract Details Scale', and the 'Quality of Parent-Child Relationships Scale' with a demographic questionnaire. Specifically, the Attitudes toward Filial Duty Contracts Scale consisted of the support for contract, the intention of contract, and reservation contract amount on young adult children's mind. Data were analyzed by means of descriptive statistics and multiple regression analysis. Results: The major findings of this study are as follows: First, young adult children reported the relationship with mother better than father's and mid levels of support and intention of filial duty contract. They also showed that their reservation contract amount on their mind was about 500 million won. Second, the multiple regression analyses revealed that gender and pride of one's father positively related to their support for filial contract. Also, gender, the pride of one's father, and emotional closeness to one's father significantly affected their intention of filial contract. In addition, among the variables, only monthly family income significantly predicted their reservation contract amount. Finally, the multiple regression analyses revealed that birth order and gender significantly predicted the normative duty contract. Also, the pride of one's father significantly affected the emotional support contract. However, filial duty contract details such as caregiving for sick parents, physical support, and economic support have been found to be meaningless. Conclusions: The findings suggest that preparative education of aging for parents who are considering making the filial duty contracts with their children should include some realistic advice. These pieces of advice include their parent-child relationship and consideration of their own overall asset sizes.

• Korean Journal of Social Welfare Studies
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• v.47 no.4
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• pp.151-181
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• 2016

The objective of the study is to identify the sub-path of entry into homelessness of young men and enhance understanding them. In specific, homeless risk factors were extracted through their experiences, and based on which the entry paths into homelessness were drawn. The homeless young adults were divided into two groups of those who became homeless in their adolescence and those who entered into homelessness after 19 and their differences were analyzed. As a results, the most prominent differences between the two sub-groups included individual and social factors; most of those who entered homelessness after 19 had physical or psychological problems, while the other group showed delinquency factors in adolescence and adulthood. As for social factors, the former group experienced more financial damage in the process of achieving independence before being homeless, while the latter group had relatively more experience of physical and psychological damage such as bullying, harassment and violence before being homeless and financial frauds. As for investigating the causality relationship of key homeless risk factors by adding a variable of time, and based on which, a constant pattern of entry paths toward homelessness was drawn among each sub-group of homeless young adults. For policy on homelessness, it is required to develop according to the characteristics of homeless young adults. Especially, a specific approach is necessary for the different homeless pathways by sub-group. Furthermore, the study indicated that management and preemptive responses for various homeless risk factors that homeless young adults experienced throughout their lifetime could be important prevention policy against homelessness.

• Korean journal of applied entomology
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• v.60 no.4
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• pp.339-355
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• 2021

Aphids can damage plants directly by absorbing their phloem sap and indirectly by transferring plant viruses and causing sooty mold. We compared the thermal effect on the development, survivorship, and reproduction of four cereal crop-damaging aphid species, Rhopalosiphum padi, Aulacorthum solani, Aphis craccivora, and Acyrthosiphon pisum using a life table analysis method. We investigated the stage-specific development period, survivorship, adult longevity, and fecundity of the above mentioned four aphids at 10, 15, 20, 25, and 30℃, respectively, and analyzed their life table parameters using the age-stage, two-sex life table analysis. A. solani nymphs could not complete their development to adulthood at 30℃. The intrinsic increase rate of R. padi was the highest at all tested temperatures except for that at 15℃ (0.12, 0.34, 0.47, and 0.32 at 10, 20, 25, and 30℃, respectively), and that of A. pisum displayed negative values at 30℃ (-0.04). It is speculated that R. padi would be a dominant species under high temperature conditions and A. solani is a highly adaptive species at low temperatures through the comparative analysis of the life table parameters of four aphid species damaging to cereal crops.

• Laboraroty Animal Research
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• v.34 no.4
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• pp.302-310
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• 2018

CD47 (integrin-associated protein), a multi-spanning transmembrane protein expressed in all cells including red blood cells (RBCs) and leukocytes, interacts with signal regulatory protein ${\alpha}$ ($SIRP{\alpha}$) on macrophages and thereby inhibits phagocytosis of RBCs. Recently, we generated a novel C57BL/6J CD47 knockout ($CD47^{-/-}$ hereafter) mouse line by employing a CRISPR/Cas9 system at Center for Mouse Models of Human Disease, and here report their hematological phenotypes. On monitoring their birth and development, $CD47^{-/-}$ mice were born viable with a natural male-to-female sex ratio and normally developed from birth through puberty to adulthood without noticeable changes in growth, food/water intake compared to their age and sex-matched wild-type littermates up to 26 weeks. Hematological analysis revealed a mild but significant reduction of RBC counts and hemoglobin in 16 week-old male $CD47^{-/-}$ mice which were aggravated at the age of 26 weeks with increased reticulocyte counts and mean corpuscular volume (MCV), suggesting hemolytic anemia. Interestingly, anemia in female $CD47^{-/-}$ mice became evident at 26 weeks, but splenomegaly was identified in both genders of $CD47^{-/-}$ mice from the age of 16 weeks, consistent with development of hemolytic anemia. Additionally, helper and cytotoxic T cell populations were considerably reduced in the spleen, but not in thymus, of $CD47^{-/-}$ mice, suggesting a crucial role of CD47 in proliferation of T cells. Collectively, these findings indicate that our $CD47^{-/-}$ mice have progressive hemolytic anemia and splenic depletion of mature T cell populations and therefore may be useful as an in vivo model to study the function of CD47.

• 한국노년학
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• v.38 no.4
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• pp.963-979
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• 2018

The purpose of this study was to identify frailty profiles based on physical, psychological, and social domains of functioning and to examine the associated factors showing the differences among frailty profiles. Respondents were 70 years and older(n=403) and latent class analysis was applied to determine the optimal subgroups based on Tilberg Frailty Indicators which comprised of three domains(the physical, psychological, and social domain). Also, we performed multinominal logistic regression analysis to find out factors making differences among frailty profiles. Latent class analysis(LCA) identified three distinct types: multi-frail type(27.0%), psychologically frail type(26.8%), inadequate support type(46.2%). All three types had common difficulties in dealing with daily life problems and did not receive enough help with theses difficulties. Based on the results of the LCA three-class models, people in multi-frail type accumulated problems in physical and psychological domains and had partially social domain. On the other hands, psychologically frail type showed a relatively high anxiety disorder and depression. Lastly, people in inadequate support type reported the lack of helps, but they were relatively healthy. Comparing these groups with inadequate support type, people with multi-frail had lower educational level, poor nutritional management status and were less likely to participate in labor market. People in psychologically frail type were more likely to be male, to live in big cities rather than middle and small cities, and less likely to smoke. Based on these results, our results showed the multifaceted concept of frailty among Korean elderly people and we suggested several implications for preventing frail process.

• Annals of Surgical Treatment and Research
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• v.95 no.5
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• pp.286-296
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• 2018

Purpose: Sentinel lymph node (SLN) biopsy (SLNB) is widely accepted for staging of melanoma patients. It has been shown that clinico-pathological features such as Breslow thickness, ulceration, age, and sex are better predictors of relapse and survival than SLN status alone. The aims of this study were to evaluate the long-term (10-year) prognostic impact of SLNB and to determine predictive factors associated with SLN metastasis, relapse, and melanoma specific mortality (MSM). Methods: This was a prospective observational study on 289 consecutive patients with primary cutaneous melanoma who underwent SLNB from January 2000 to December 2007, and followed until January 2014, at an Italian academic hospital. Results: SLN was positive in 64 patients (22.1%). The median follow-up was 116 months (79-147 months). Tenyear disease-free survival and melanoma specific survival were poor in patients with positive SLN (58.7% and 66.4%, respectively). Only the increasing Breslow thickness resulted independently associated to an increased risk of SLN metastasis. Cox regression analysis showed that a Breslow thickness >2 mm was an independent predictor of relapse, and male sex and Breslow thickness >2 mm was a predictor of MSM. At 10 years, SLN metastasis was not significantly associated to either relapse or MSM. Conclusion: After the fifth year of follow-up, SLN metastasis is not an independent predictive factor of relapse or mortality which are mainly influenced by the characteristics of the primary tumor and of the patient. Patients with a Breslow thickness >2 mm regardless of the SLN status should be considered at high risk for 10-year relapse and mortality.

• Korean Journal of Comparative Education
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• v.28 no.4
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• pp.189-220
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• 2018

The purpose of this study is to analyze the effect of individual background, educational experiences, and social awareness on youth's civic participation in the three East Asian countries (South Korea, Hong Kong, and Taiwan), and provide educational implications for encouraging Korean youth's civic participation. And we estimated the effects of 17 variables on the youth's current and future civic participation using 'International Civic and Citizenship Education Study (ICCS) 2016' data opened in 2018. The result showed that the factors affecting on the civic participation were significantly different between the three countries. And significant differences on current and future civic participation were confirmed within the same country. First, Korean youth have high level of their parents' civic resources but the level of civic education in school is lower than other countries, and Taiwan youth have relatively high level of national identity. Second, the factors affecting on civic participation are more influenced by educational experiences than individual background such as parents' socioeconomic status, and the mass media have significant effect on youth's civic participation in all the three countries. Third, non-formal program and cultural experiences in school have significant effects on civic participation in future adulthood. Based on these results, this study suggested that it was important to provide various non-formal activities as well as expansion of civic education for encouraging youth's civic participation.

• Journal of vocational education research
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• v.37 no.4
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• pp.67-89
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• 2018

The purpose of this study is to analyze the role of career flexibility on employment status, career growth, and job satisfaction longitudinally. Demographic information(gender, age, parent's income level, and students' perceived financial states of family), vocational identity, employment status, career growth, and job satisfaction at 1, 2, and 3 years after the first data collection with a sample of 259(time 1), 229(time 2), 182(time 3) were collected respectively. Multivariate regression and logistic regression analysis were performed. Results showed that in-breadth career exploration positively predicted to employment status at time 1, 2, 3 consistently and career flexibility also positively predicted to employment status at time 2, 3. The longitudinal effect of career flexibility was found that the expectancy of career flexibility had shown to have stronger effect. In addition, career growth was influenced by in-depth career exploration at time 2, 3. On the other hand, no variables were related to job satisfaction in this study. Results of the current study provided the new insights for career education and limitation and future suggestions were discussed.

• Nutrition Research and Practice
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• v.13 no.4
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• pp.352-357
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• 2019

BACKGROUND/OBJECTIVES: Youth is the crucial stage between childhood and adulthood during which an individual acquires new behavior and practices including dietary habits, that may be influenced by his peers, social circle, nutrition knowledge level and other factors. The increase in awareness of young adults is of special importance from the perspective of health and prevention of obesity related chronic diseases. To measure the nutrition literacy level of university students using the Turkish version of Adolescent Nutrition Literacy Scale (ANLS). We evaluated their food habits using the Adolescent Food Habit Checklist (AFHC) tool and analysed it, if the eating habits were affected by nutrition literacy level. SUBJECTS/METHODS: A cross-sectional descriptive study was conducted on randomly selected 276 students studying in the Faculty of Health Sciences of Istanbul Aydin University, Turkey. Data was collected by means of ANLS and AFHC tools by face to face interview method. SPSS statistical sofware (IBM SPSS Statistics 19) was used to analyse the obtained data. RESULTS: Participants comprised of 47.1% males and 52.9% females with mean age of $20.0{\pm}1.60years$. Mean body mass index (BMI) was determined as $22.4{\pm}3.76$ ($23.6{\pm}3.63$ males $21.3{\pm}3.57$ females). Functional, interactive and critical nutritional literacy scores for male participants were $22.49{\pm}5.71$, $17.45{\pm}4.84$, and $28.28{\pm}7.13$ respectively (total $64.98{\pm}10.15$). For females the values were $24.66{\pm}5.13$, $20.17{\pm}4.28$, and $32.20{\pm}5.65$ (total $69.72{\pm}8.59$) respectively. For all the three sub-categories of nutrition literacy, the scores acquired by females were significantly higher than males (P < 0.001). In the AFHC tool, the mean score acquired by males were $9.26{\pm}4.18$ whereas for females it was $10.37{\pm}3.40$ significantly higher as compared to males (P = 0.007). CONCLUSION: The food habits of female participants were better than males; in accordance with their nutrition literacy status. It may be concluded that food habits are influenced by nutrition literacy and therefore stress much be given to increase the nutrition awareness among children youth as well as adults.