• Archives of Pharmacal Research
• /
• v.12 no.2
• /
• pp.63-67
• /
• 1989

The various esters of 20R and 20S-dihydroprednisolonic acid were prepared and evaluated for their local and topical anti-inflammatory activities in rats, including systemic effects. It was demonstrated that the size of the substituent at C-21 and configurational arrangement at C-20 have significant effects on local and topical activity. It was also found that all the derivatives did not show systemic effects as accessed by thymus weight and adrenal weight at the dose level used, while prednisolone showed profound effects on thymus weight.

• Korean Journal of Veterinary Pathology
• /
• v.5 no.2
• /
• pp.39-42
• /
• 2001

A reference range data base containing organ weight values on animals used in 4 week and 13 week toxicity tests is described. Data listed include the values of minimum, maximum, mean and standard deviation for each of the following organ weights. Organs: Brain, heart, liver, spleen, kidney, adrenal gland, testis, ovary. This study was undertaken to determine organ weight reference values of Ktc: SD rats

• Korean Journal of Veterinary Research
• /
• v.44 no.4
• /
• pp.523-532
• /
• 2004

The present study was carried out to investigate the potential adverse effects of amitraz on pregnant dams after maternal exposure during the gestational days (GD) 1 through 19 in Sprague-Dawley rats. The test chemical was administered orally to pregnant rats at dose levels of 0, 3, 10, or 30 mg/kg/ day. During the test period, clinical signs, mortality, body weights, food consumption, serum biochemistry, gross findings, organ weights and reproductive findings on GD 20 were examined. In the 30 mg/kg group, an increase in the incidence of abnormal clinical signs and death, a suppression in the body weight gain, and a decrease in the food consumption were observed. A decrease in the liver weight and increases in the kidneys, adrenal glands and heart weights were also found. Serum biochemical investigations revealed increases in the aspartate aminotransferase (AST), total bilirubin, and chloride. In addition, an increase in the fetal death and decreases in the litter size and fetal body weight were seen at caesarean section. Inthe 10 mg/kg group, an increase in the incidence of abnormal clinical signs, decreases in the food consumption and liver weight, increases in the total bilirubin and chloride, and a decrease in the fetal body weight were observed. There were no adverse effects on clinical signs, mortality, body weights, food consumption, serum biochemistry, gross findings, organ weights and reproductive findings in the 3 mg/kg group. Based on the results, it was concluded that the 19-day repeated oral dose of amitraz to pregnant rats caused increases in the clinical signs, kidneys, adrenal glands and heart weights, AST, total bilirubin and chloride and decreases in the body weight gain, food consumption and liver weight at the dose levels of above 10 mg/kg/day. Under the present experimental conditions, the no-observed-adverse-effect level (NOAEL) of amitraz was considered to be 3 mg/kg/day.

• The Journal of Internal Korean Medicine
• /
• v.22 no.4
• /
• pp.683-689
• /
• 2001

In order to study the anti-stress effect of hyangbujapalmultang, several measures of stress, including body weight changes, organ weight changes and catecholamine changes. The following result have been obtained: 1. The weight loss of contol group was $28.5{\pm}1.8g$, that of sample group was $20.3{\pm}1.6g$. This differance was statistcally significant. 2.The organ weight(Liver,Spleen,Kidney,Adrenal gland) was the only significant change in the spleen, in sample group comparing to control group. 3. The norepinephrine contents of control group was $695.5{\pm}22.7pg/ml$, that of sample group was $607.4{\pm}21.7pg/ml$. This shows significant difference in sample group comparing to contol group. 4. The epinephrine contents of control group was $212.8{\pm}9.8pg/ml$, that of sample group was $182.6{\pm}8.4pg/ml$. This shows significant differance in sample group comparing to control group. 5. The dopamine contents of control group was $504.5{\pm}31.3$, that of sample group was $463.4{\pm}27.8pg/ml$. This shows the value decreased, but none of it is significant. Based on avove results, it may be conculed that hyangbujapalmultang has anti-stress effects.

• Journal of Korean Physical Therapy Science
• /
• v.13 no.2
• /
• pp.67-83
• /
• 2006

The present study examined that in Vivo/Vitro test is investigated in normotensive sham-operated rats (NSR) and aldosterone-analogue deoxycorticosterone acetate (DOCA)-salt hypertensive rats (ADHR) and that the antihypertensive effect was induced by silver spike point (SSP) electrical stimulation at meridian points(CV-3, -4, Ki-12, SP-6, LR-3, BL-25, -28, -32, -52), specifically, such as aldosterone in 24 hour urine analysis from healthy volunteer. The gross examination and morphometric-histological changes, such as hypertrophy, production of necrotic tissues, and the changes of cell arrangement on the kidney, and adrenal gland were markedly observed in aldosterone-analogue DOCA-salt hypertensive rats compared with those from normotensive sham-operated rats. The systolic blood pressure, weight of kidney and adrenal gland were significantly increased in ADHR than that in NSR. The required time of PSS-induced resting tone was significantly increased in ADHR than that in NSR. However, the voltage-dependent K+ (Kv) currents were significantly decreased in ADHR than that in NSR. The urine analysis showed that the concentration of aldosterone was significantly decreased in resting state from the elderly people compared with those from the adolescent healthy volunteer. The current of 1 Hz continue type of SSP electrical stimulation significantly decreased in the concentration of aldosterone of 24 hour urine from the elderly people. These results suggest that the development of aldosterone analogue-induced hypertension is associated with changed the weight of kidney and adrenal gland, blood pressure, resting tone and Kv currents, which directly affects blood pressure. Therefore, the hypertension is a risk factor on cerebrovascular disease. Moreover, these results suggest that the diminished responsiveness to SSP electrical stimulation, especially current of 1Hz continue type, in elderly people may be, in part, related by the increased of antihypertensive effects.

• Toxicological Research
• /
• v.11 no.1
• /
• pp.91-101
• /
• 1995

A perinatal and postnatal study of KTC-1, a new semisyntheitic rifamycin antituberculous drug, was conducted in Sprague-Dawley rats. Dosages of KTC-1 0, 12, 27.6, and 63.5 mg/kg/day were administered to dams orally by gavage from day 17 of gestation to day 21 of lactation. All pregnant rats were allowed to deliver naturally for postnatal examination of their offspring. At 63.5 mg/kg/day, weakness, dark-red discharge around eyes, a loss in body weight, and a decrease in food and water consumption were observed in dams. An increase in the weight of adrenal gland and spleen, and a decrease in the weight of kidney and heart were also found. An increase in neonatal deaths during the lactation period, a loss in body weight, a delay in physical development, a decrease in traction ability, an increase in the number of errors and the time required for the multiple T-maze trial were found in F1 offspring. In addition, an increase in the incidence of visceral variations and retarded ossification were observed in F1 4 day old rats. An increase in the incience of skeletal anomalies was seen in F2 fetuses. There were no sings of maternal toxicity or embryotoxicity at 12 and 27.6 mg/kg/day. From the results mentioned above, it can be concluded that the no-effect dose levels(NOELs)for dams, F1 offspring, and F2 fetuses are 27.6 mg/kg/day.

• Current Research on Agriculture and Life Sciences
• /
• v.3
• /
• pp.181-189
• /
• 1985

This study was conducted to determine the effect of the adrenal function on the reproductive organs in immature rats treated with PMS. Two hundred and ten female rats (Wistar-Imamichi albino rats) of 21 days old (body weight : $58.7{\pm}3.53g$) were disposed in the intact rat group (Int.-) and adrenalectomized rat group (Adx.-) and then each group was devided into 3 subgroups, such as control (-Cont.), PMS treated (-PMS) was administered subcutaneously with 25 IU PMS, and and PMS cortisol treated groups (-PMS+Corti.) with 25 IU PMS and $30.0{\mu}g$ cortisol on 5 th day (aged 26 days old) after adrenalectomy, while the control groups with physiological salt solution by the same way. The reprodutive organs were observed at 48, 54, 60, 66, 72, 78 and 84 hours after hormone treatments. The results obtained were as follows ; 1. The measurments of time average ovary weight in all treated groups were increased with the elapse of time after treatment, and the difference among the treatments was significant (p<0.01) in the all observation time. But the difference of those was not recognized in Int.-Cont. and Adx.-Cont. groups. In the multiple range test. ovary weight of adrenalectomized rat groups (Adx.-PMS and Adx.-PMS+Corti. groups) was significantly (p<0.05) lighter than those of intact rat groups (Int.-PMS and Int.-PMS+Corti. groups), and the effect of cortisol administration was not reconized. 2. The difference of uterus weight was significantly reconized (p<0.01) in all observation time. The weight in Int.-PMS and Int.-PMS+Corti. groups was heavier until 66 hours after treatment, but the values in the adrenalectomized Adx.-PMS and Adx.-PMS+Corti. groups were heavier after 72 hours. The multiple range test showed that the significant difference was not found between Int.-PMS and Int.-PMS+Corti. groups, and Adx.-PMS and Adx.-PMS+Corti. groups. 3. The adrenal weight was not significantly different among the compared groups.

• Asian-Australasian Journal of Animal Sciences
• /
• v.20 no.2
• /
• pp.263-272
• /
• 2007

This experiment was conducted to examine the effects of cage type (CT) and cage density (CD) on physiological variables in growing rats. Sprague Dawley rats (n=108) weighing an average of 46 g were housed in metallic cage with woodchip bedding (MCWB), metallic cage with wire mesh (MCWM), and plastic shoebox with woodchip bedding (PCWB) separately by sex at normal ($160-cm^2/rat$, ND) and high ($80-cm^2/rat$, HD) CD from 3 to 10 wks of age. All cages were in dimension of $24{\times}40{\times}20$ cm ($W{\times}D{\times}H$). At the end of the experiment, blood samples were collected and 6 rats from each cage were sacrificed. No death was observed among rats at ND, whereas mortality rate at HD was 22.3% for males and 13.9% for females. Heart weight was affected by CT. Doubling CD caused 23, 11.8, 17.9, 8.6, 6.9, and 16.4% decreases in BW and weights of heart, liver, kidney, testis, and ovary, respectively. Except for adrenal gland, other organs for males were heavier than for females. Liver weight of males and females responded differently to CT and CD. Comparing with females, males had 7.3 and 5.2% heavier and 9.9% lighter liver weights in MCWB, MCWM, and PCWB, respectively. As CD doubled, liver weight for males and females decreased by 22.4 and 13.1%, respectively. Mean adrenal gland weight increased by 8.4% and decreased by 9.7% for males and females, respectively, with doubling CD. CT affected glucose, TG, Ca, and ALP levels. However, CD did not alter blood chemistry. Rats housed in metallic cages had greater neutrophil count and neutrophil:lymphocyte ratio than rats housed in plastic cages. Doubling CD caused a 24.2% increase in lymphocyte count. There were CT by CD, CT by sex, and CD by sex interaction effects on lymphocyte count. Doubling CD caused 0.1% decrease and 49.8 and 26.7% increases in lymphocyte count for rats housed in MCWB, MCWM, and PCWB, respectively. Comparing with females, lymphocyte count for males housed in MCWB, MCWM, and PCWB had 8.9 and 12.9% greater and 30.3% less lymphocyte counts, respectively. Lymphocyte count decreased by 4.12% for males, whereas it increased by 61.0% for females as CD doubled. Doubling CD resulted in 2.5 and 2.3% increases in erythrocyte count and hematocrit value. These data suggest that animals perform better in metallic cages than in plastic cages and that cage density had pronounceable effects on physiological parameters in a cage type and sex dependent-manner.

• Nutrition Research and Practice
• /
• v.1 no.4
• /
• pp.291-297
• /
• 2007

This study was conducted to investigate the effect of dietary soluble fiber administration and/or high fat diet on serum and brain neurohormonal profiles, adipose tissue mass and body weight gain in Sprague-Dawley rats. Four groups of rats were respectively fed 10% fat diet (C), 10% fat plus pectin diet (P), 20% fat diet (HFC) and 20% fat plus pectin diet (HFP) for 4 weeks. In HFP group, the food and energy intake, body weight gain, FER including fecal excretion were the smallest (p<0.05). Serum HDL-cholesterol, triglyceride and glucose level were also the lowest in HFP group (p<0.05). The weight of brain, epididymal fat pad and adrenal gland except liver didn't show any significant differences among groups. Interestingly serum norepinephrine concentration of HFP group tended to be higher, but dopamine concentration tended to be lower than those of HFC group. However serum catecholamine concentration didn't show any significant differences among all groups. Norepinephrine and epinephrine contents of right portion of midbrain of P and HFP groups were remarkably lower than those of the C group. These results suggested that soluble fiber pectin consumption might affect neurohormonal profiles in serum and brain according to dietary fat level.

• Journal of Nutrition and Health
• /
• v.8 no.3
• /
• pp.31-37
• /
• 1975

The effect of dietary restriction was obserbed in Albino rats. Dietary restriction was at 2 levels: 10%, 20%, 30 males and same number of females of Albino rats, aged $30{\sim}40$ days were devided into following 6 groups, 5 rats each. 1 group; rice standard group 2 group; 10% restriction of rice standard 3 group; 20% restriction of rice standard 4 group; 65% rice+35% chinese cabbage group 5 group; 10% restriction of 65% rice+35% chinese cabbage 6 group; 20% restriction of 65% rice+35% chinese cabbage The rats were kept in individual cage and given 6 different diet for 7 weeks. The results of this study were elucidated as follow. 1. Final body weight was not obserbed any significant differences between non-restriction group and 10% restriction group, but 20% restriction group revealed the decrease of body weight. 2. According to dietary restriction, final organ weight was almost decreased in the restriction group of rice+chinese cabbage, and in rice standard group, there were no significants differences between none-restriction group and 10% restriction group, but organ weight was sharply decreased such as Liver, Heart, Kidney, Adrenal, Spleen in 20% restriction group. Sex organ weight of female was increased but weight of male was decreased in 20% restriction group of rice standard group. 3. The result of biochemical analysis such as nitrogen retention, serum cholesterol, glucose of urine, feces, and serum, and lipid of feces and liver, was not revealed any significant diffences, but in 20% restriction group, urinary glucose and serum glucose levels were somewhat decreased, and liver nitrogen level was increased. 4. Therefore there is no effect on albino rats by 10% restricted diet. It can be concluded that it is possible to restrict the diet within 10%.