• 제목/요약/키워드: adhesion to Caco-2

검색결과 27건 처리시간 0.027초

비피도박테리아의 Caco-2 세포에 대한 부착성과 세포 표면 소수성 (Adhesion of Bifidobacteria to Caco-2 Cells and in Relation to Cell Surface Hydrophobicity)

  • 임광세;허철성
    • 한국축산식품학회지
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    • 제26권4호
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    • pp.497-502
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    • 2006
  • 한국인 유아의 분변에서 분리한 10종의 분리균주를 포함하여 총 16종의 bifidobacteria에 대한 Caco-2 세포에 대한 부착성과 세포 표면 소수성을 측정하였다. 부착성과 세포 표면소수성 모두 균주에 따라 상이한 결과를 나타내었으며 균종에 따른 경향은 관찰되지 않았다. 실험 균주 중에서 B. longum D6, B. longum H4, B. breve Ml, B. thermophilum ATCC 25525, B. suis ATCC 27533, B. animalis subsp. lactis BB12 균주가 세포 표면 소수성이 높게 나타났으며, Caco-2 세포에 대한 부착성은 B. bifidum B3, B. longum D6, B. longum H4, B. thermophilum ATCC 25525, B. suis ATCC 27533, B. animalis subsp. lactis BB12, B. longum 2 등이 우수하였다. 또한 Caco-2세포에 200개 이상이 부착되는 실험 균주는 모두 60% 이상의 세포 표면 소수성을 나타내고 있으므로, 부착성이 우수한 균주 선발 시 분리 균주의 세포 표면 소수성을 측정하여 선발하는 것이 가능하다고 판단되었다.

Inhibitory Effect of Lactobacillus plantarum K11 on the Adhesion of Escherichia coli O157 to Caco-2 Cells

  • Lim, Sung-Mee;Ahn, Dong-Hyun;Im, Dong-Soon
    • Food Science and Biotechnology
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    • 제18권2호
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    • pp.343-349
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    • 2009
  • Inhibitory effect of Escherichia coli O157 adhered to Caco-2 cells by the cells of Lactobacillus plantarum K11 and the cell-free culture supernatant (CFCS) and bacteriocin prepared from this strain was investigated. As the cell counts of viable L. plantarum K11 previously adhered to Caco-2 were increased, the rate of adhesion and adherent cell counts of E. coli O157 was lower. However, because the heated L. plantarum K11 rarely have the adhesion ability to Caco-2, the adhesion rate and adherent cell counts of E. coli O157 were high. In addition, the inhibitory effects of E. coli O157 adhesion by the CFCS and bacteriocin of L. plantarum K11 were dose-dependent manner. Therefore, the inhibition of adhesion of E. coli O157 to Caco-2 may result from the antimicrobial substances such as lactic acid and bacteriocin. Moreover the inhibitory activity of adhesion by the heated bacteriocin for 30 min at 100oC was similar to activity of non-treated bacteriocin, but the activity was disappeared by treatment with protease.

Characterization of Adhesion of Bifidobacterium sp. BGN4 to Human Enterocyte-Like Caco-2 Cells

  • Kim, In-Hee;Park, Myung-Soo;Ji, Geun-Eog
    • Journal of Microbiology and Biotechnology
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    • 제13권2호
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    • pp.276-281
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    • 2003
  • The adhesion of probiotic bacteria to the intestinal mucosa is one of the desirable properties for their colonization in the intestinal tract, where these bacteria constantly compete with other bacteria. The adhesion of different strains of bifidobacteria to Caco-2 cells was compared. Among the strains examined, BGN-4 showed the highest adhesion level and the greatest cell surface hydrophobicity (CSH). No close relationship was found between the adhesion and CSH of the strains. Upon protease and heat treatment, the adhesion of the BGN-4 to the Caco-2 cells decreased significantly. The cells grown at $42^{\circ}C$ showed a lower CSH and self-aggregation levels than cells grown at $37^{\circ}C$. The treatment of EGTA did not have any effect on the adhesion. The degree of adhesion did not differ among the experimental groups in which galactose, mannose, or fucose were added in the adhesion assay mixture. The results suggest that the adhesion of the Bifidobacterium to the epithelial cells may be affected by the composition and structure of the cell membrane and interacting surfaces.

Factors Affecting Adhesion of Lactic Acid Bacteria to Caco-2 Cells and Inhibitory Effect on Infection of Salmonella Typhimurium

  • Lim, Sung-Mee;Ahn, Dong-Hyun
    • Journal of Microbiology and Biotechnology
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    • 제22권12호
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    • pp.1731-1739
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    • 2012
  • In this study, seven strains isolated from mustard leaf kimchi were screened for their tolerance to simulated gastric and bile juices, the adhesive properties to Caco-2 cells, and the inhibition ability of Salmonella Typhimurium ATCC 29631 adhesion. Lactobacillus acidophilus GK20, Lactobacillus paracasei GK74, and Lactobacillus plantarum GK81, which were resistant to bile as well as gastric juices, possessed high bile-salt hydrolase (BSH) activity towards both sodium glycocholate and sodium taurocholate. The strongest in vitro adherence of $53.96{\pm}4.49%$ was exhibited by L. plantarum GK81 followed by L. acidophilus GK20 with adhesion levels of $40.72{\pm}9.46%$. The adhesion of these strains was significantly (p < 0.05) reduced after exposure to pepsin and heating for 30 min at $80^{\circ}C$. Addition of $Ca^{2+}$ led to a significant (p < 0.05) increase of the adhesion of L. acidophilus GK20, but the adhesion ability of L. plantarum GK81 was not different from the control by the addition of calcium. In the competition and exclusion experiment, the adhesion inhibition of S. Typhimurium by L. plantarum GK81 strain was much higher than the other strains. Moreover, the exclusion inhibition of S. Typhimurium by L. acidophilus GK20 was considerably high, although the inhibition activity of this strain was lower than L. plantarum GK81.

Adhesion Properties of Indigenous Dadih Lactic Acid Bacteria on Human Intestinal Mucosal Surface

  • Dharmawan, Jorry;Surono, Ingrid S.;Kun, Lee Yuan
    • Asian-Australasian Journal of Animal Sciences
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    • 제19권5호
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    • pp.751-755
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    • 2006
  • Dadih is Indonesian traditional fermented buffalo milk believed by the natives to have beneficial effects on human health. This may be due to the probiotic properties possessed by the lactic acid bacteria (LAB) involved in its fermentation process. It was discovered that ten strains of dadih lactic isolates possessed some probiotic properties in vitro. In this study, the adhesion properties of dadih LAB, in comparison with documented probiotic strains, were investigated in vitro by using mucin extracted from human faeces and Caco-2 cells as the models for human intestinal mucosal surface and intestinal cells respectively. The adhesion results showed the distinction of Lactobacillus reuteri IS-27560 in adhering to both mucus layer and Caco-2 cells. The competition assay for adhesion to the mucus layer between dadih LAB and selected pathogens indicated the competence of Lactococcus lactis IS-16183 and Lactobacillus rhamnosus IS-7257 in significantly inhibiting the adhesion of Escherichia coli O157:H7. Accordingly, these two strains may be potential candidates for use as probiotic strains. Overall, the adhesion properties of all dadih LAB strains were relatively comparable to that of Lactobacillus casei Shirota and Lactobacillus rhamnosus GG, the documented probiotic strains.

Screening and Characterization of Pro biotic Lactic Acid Bacteria Isolated from Korean Fermented Foods

  • Lim, Sung-Mee;Im, Dong-Soon
    • Journal of Microbiology and Biotechnology
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    • 제19권2호
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    • pp.178-186
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    • 2009
  • To examine their potential as probiotics, acid and bile tolerance, antibiotics resistance, adhesion capacity to Caco-2 and HT-29, and antibacterial activity, of LAB isolated from Korean fermented foods such. as dongchimi, kimchi, Meju, and doenjang were assayed against foodborne pathogenic bacteria. DC 55, DC 136, DC 222, KC 21, KC 24, KC 34, KC 43, KC 117, MJ 54, MJ 301, SP 33, and SP 170 strains were resistant to acid and bile conditions. In particular, DC 55, DC 136, KC 24, KC 43, and MJ 301 strains were highly resistant to higher than 20 ${\mu}g/ml$ concentrations of vancomycin, streptomycin sulfate, or amoxicillin, whereas, DC 222, KC 21, KC 34, KC 117, MJ 54, and SP 33 strains were susceptible to lower than 2 ${\mu}g/ml$ concentrations of those antibiotics. The adhesion to HT-29 and Caco-2 cells varied with the strains tested in a strain-dependent manner. The highest level of adhesion was observed with DC 55, KC 21, KC 24, and MJ 301 strains, having higher than 50% of adhesion to HT-29 or Caco-2 cells. In addition, Staphylococcus aureus was the most sensitive to KC 21, showing an inhibition of about 70%, and the antibacterial activity of KC 21 against S. aureus resulted most likely from both organic acids and bacteriocin. Based on its phenotypic characteristics and utilization of various sugars, the KC 21 strain was identified as Lactobacillus plantarum.

김치 유산균의 Caco-2 세포막 부착성 및 Aflatoxin B1 제거 효과 (Adhesion of Kimchi Lactobacillus Strains to Caco-2 Cell Membrane and Sequestration of Aflatoxin B1)

  • 이정민
    • 한국식품영양과학회지
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    • 제34권5호
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    • pp.581-585
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    • 2005
  • 김치의 발효와 숙성 에 관여하는 2종의 유산균과 3종의 유제품으로부터 분리된 유산균을 Caco-2 세포 부착성과 $AFB_1$ 흡착능에 대해 비교 검토하여 보았다. 또한 유산균을 생균군, 열처리군, 강산처리군으로 나누어 유산균의 부착성 및 흡착력이 세포벽의 구조와 관련이 있다는 보고를 재확인하고자 하였다. L. plantarum KCTC 3099의 경우 Caco-2 세포 부착성이나 $AFB_1$ 흡착능이 높게 나타났으며 이것은 양성 대조군으로 사용된 L. rhamnosus GG와 유사한 수준을 나타내었다. 하지만 L. mesenteroides KCTC 3001은 Caco-2 부착성은 다소 높게 나타났으나 $AFB_1$ 흡착능은 낮게 나타났다. 이것은 Caco-2세포에 결합하는 부위와 $AFB_1$에 결합하는 부위가 일치하지는 않는다는 것을 암시하는 것으로 사료된다. 또한 유산균의 처리방법에 따라서도 다양한 차이를 보였는데 본 실험에서는 강산처리군의 경우 보다 효과적인 것으로 나타났으며 세포벽의 주된 구조인 peptidoglycan과 polysaccharides이 강산의 처리에 의해 결합이 파괴되면서 Caco2 세포 부착성이나 $AFB_1$ 흡착능의 상승에 영향을 미쳤으리라 여겨진다. 하지만 강산처리에 의한 세포벽의 변화는 비특이적으로 발생하기 때문에 동일한 형태로 모든 유산균에 적용 되기는 어려울 것이며 각 유산균종의 세포벽 구조와 특이 성분의 함량에 따라 다양하게 변화가 일어날 것으로 사료된다.

Improving the Viability of Freeze-dried Probiotics Using a Lysine-based Rehydration Mixture

  • Arellano, Karina;Park, Haryung;Kim, Bobae;Yeo, Subin;Jo, Hyunjoo;Kim, Jin-Hak;Ji, Yosep;Holzapfel, Wilhelm H.
    • 한국미생물·생명공학회지
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    • 제49권2호
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    • pp.157-166
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    • 2021
  • The probiotic market is constantly continuing to grow, concomitantly with a widening in the range and diversity of probiotic products. Probiotics are defined as live microorganisms that provide a benefit to the host when consumed at a proper dose; the viability of a probiotic is therefore of crucial importance for its efficacy. Many products undergo lyophilization for maintaining their shelf-life. Unfortunately, this procedure may damage the integrity of the cells due to stress conditions during both the freezing and (vacuum-) drying process, thereby impacting their functionality. We propose a lysine-based mixture for rehydration of freeze-dried probiotics for improving their viability during in vitro simulated gastric and duodenum stress conditions. Measurement of the zeta potential served as an indicator of cell integrity and efficacy of this mixture, while functionality was estimated by adhesion to a human enterocyte-like Caco-2 cell-line. The freeze-dried bacteria exhibited a significantly different zeta potential compared to fresh cultures; however, this condition could be restored by rehydration with the lysine mixture. Recovery of the surface charge was found to influence adhesion ability to the Caco-2 cell-line. The optimum lysine concentration of the formulation, designated "Zeta-bio", was found to be 0.03 M for improving the viability of Lactiplantibacillus plantarum Lp-115 by up to 13.86% and a 7-strain mixture (400B) to 41.99% compared to the control rehydrated with distilled water. In addition, the lysine Zeta-bio formulation notably increased the adherence ability of lyophilized Lp-115 to the Caco-2 cell-line after subjected to the in vitro stress conditions of the simulated gastrointestinal tract passage.

Potential Probiotic Characteristics and Safety Assessment of Lactobacillus rhamnosus SKG34 Isolated from Sumbawa Mare's Milk

  • Sujaya, I Nengah;Suwardana, Gede Ngurah Rsi;Gotoh, Kazuyoshi;Sumardika, I Wayan;Nocianitri, Komang Ayu;Sriwidyani, Ni Putu;Putra, I Wayan Gede Artawan Eka;Sakaguchi, Masakiyo;Fatmawati, Ni Nengah Dwi
    • 한국미생물·생명공학회지
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    • 제50권1호
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    • pp.51-62
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    • 2022
  • Lactobacillus rhamnosus SKG34 (LrSKG34), a potential probiotic strain, was successfully isolated from Sumbawa Mare's milk. Our previous studies showed that the strain is resistant to gastrointestinal conditions, possesses antioxidant activity, and lowers blood cholesterol levels. Further clarification of the potential probiotic characteristics and safety assessment are necessary. This study aimed to evaluate the adhesion of LrSKG34 to Caco-2 cell monolayers and its effect on mucosal integrity in vitro. We also examined the LrSKG34 safety profile based on antimicrobial susceptibility testing, haemolytic activity determination, Caco-2 cell monolayer translocation evaluation, and in vivo investigation of the effect of LrSKG34 on the physiology, biochemical markers, and histopathological appearance of major organs in an animal model. LrSKG34 attached to Caco-2 cell monolayers and maintained mucosal integrity in vitro. The typical resistance of lactobacilli to ciprofloxacin, gentamicin, vancomycin, trimethoprim-sulfamethoxazole, and metronidazole was confirmed for LrSKG34. No haemolytic activity was observed on blood agar plates, and no LrSKG34 translocation was observed in Caco-2 cell monolayers. Administration of LrSKG34 to Sprague-Dawley rats did not adversely affect body weight. No abnormalities in hematological parameters, serum biochemistry levels, or histopathological structures of major organs were observed in LrSKG34-treated rats. Collectively, the results implicate LrSKG34 as a promising and potentially safe probiotic candidate for further development.

Characterization of Bacillus polyfermenticus KJS-2 as a Probiotic

  • Kim, Kang-Min;Kim, Myo-Jeong;Kim, Dong-Hee;Park, You-Soo;Kang, Jae-Seon
    • Journal of Microbiology and Biotechnology
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    • 제19권9호
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    • pp.1013-1018
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    • 2009
  • The identification and characterization of Bacillus polyfermenticus KJS-2 (B. polyfermenticus KJS-2) was conducted using TEM, an API 50CHB kit, 16S rDNA sequencing, a phylogenetic tree, and catalase and oxidase testing. The conversion rate of glucose to lactic acid by B. polyfermenticus KJS-2 was found to be $60.7{\pm}4.9%$. In addition, treatment of B. polyfermenticus KJS-2 with artificial gastric juice (pH 2.0) and bile acid (pH 6.5) for 4 h resulted in a final viability of $140{\pm}7.9%$ and $108{\pm}3.5%$, respectively. Finally, the results of adhesion experiments using Caco-2 cells revealed that the adherence of B. polyfermenticus KJS-2 to Caco-2 cells was approximately $65{\pm}0.6%$.