• Journal of Genetic Medicine
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• v.17 no.1
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• pp.34-38
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• 2020

The term dopa-responsive dystonia (DRD) is used to describe a group of neurometabolic disorders, which are characterized by dystonia, and are typically associated with diurnal fluctuations and respond to levodopa treatment. Autosomal dominant DRD (DYT5a, MIM# 128230) is caused by a heterozygous mutation in the GTP cyclohydrolase 1 (GCH1) gene (MIM# 600225). GCH1 encodes an enzyme, which is involved in the biosynthesis of tetrahydrobiopterin, an essential co-factor for tyrosine hydroxylase. Herein, we report the case of a 16-year-old girl who was diagnosed with DYT5a. She exhibited additional unusual clinical features, including intellectual disability, depression, multiple skeletal anomalies, and short stature, which are not commonly observed in patients with DYT5a. The patient harbored a heterozygous missense variant, c.539A>C, p.Gln180Pro, in the GCH1 gene, which was identified by targeted gene panel analysis using next-generation sequencing.

• Journal of Life Science
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• v.19 no.5
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• pp.561-565
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• 2009

Human Tissue factor is an essential enzyme activator that forms a catalytic complex with factor VII/ VIIa, and catalyzes both the extrinsic and intrinsic blood coagulation cascades. The extracellular domain of human tissue factor is responsible for association with the biological partner. The efficient procedures for preparing biologically active human tissue factor are essential for the preclinical and clinical studies with coaguligands. An expression vector in Escherichia coli has been constructed to direct the production of extracellular human tissue factor without a fusion protein or a $His_6$ at the N-terminus. The recombinant human tissue factor was expressed in large amounts as a non-native state in E. coli. The recombinant protein was simply renatured during the DEAE-sephacel chromatographic purification procedure. Our expression and purification system does not require a protease treatment or an additional chromatographic step to remove a fusion contaminant, which provides a very useful alternative to conventional expression systems for the production of human tissue factor.

• The Journal of Information Systems
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• v.11 no.1
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• pp.51-74
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• 2002

The purpose of this study is to empirically investigate critical success factors for effective implementation of web-based distance learning system. First of all, four critical success factors are theoretically derived from reviewing previous research. They are: (1) learner-related factor including the variables such as teaming ability, learning attitude, and attending motivation, (2) environmental factor including the variables of physical and mental support for learners, (3) instructional design factor represented by one variable, the degree of appropriateness of learning contents, and (4) the factor concerning the level of self-directed learning readiness embracing the variables such as curiosity for learning, openness towards challenge of learning and affection for learning. Subsequently, the relationships between these four critical success factors and the degree of learning satisfaction are empirically investigated. The data for empirical analysis of the research are collected from 1,020 respondents who have already passed the web-based distance learning courses which have been implemented in Information and Communication Officials Training Institute. Out of 1,020 responded questionnaires, 875 data were available for statistical analyses. The main results of this study are as follows. Firstly, the most important factor for successful implementation of the web-based distance learning system is shown to be the instructional design factor, and in the next place, the self-directed learning readiness factor, the environmental factor and the learner-related one in sequence. Secondly, additional analysis of the variables included in the instructional design factor shows that availability of practical information and knowledge is the most influencing variable, and next, interesting composition of contents, reasonable learning amount, optimal level of instruction, and understandable explanation are significantly important in the descending order. Lastly, among learning motivators, strong intention of acquiring business knowledges and skills is found to be the most important satisfier in the web-based distance learning. The theoretical contribution of this study is to derive a comprehensive model of critical success factors for implementing the web-based distance learning system. And, the practical implication of this study is to propose efficient and effective guidelines for developing and operating the web-based distance learning system in the various kinds of organizations.

• Journal of Microbiology
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• v.45 no.6
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• pp.534-540
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• 2007

The Streptomyces coelicolor A3(2) genome contains six operons (rrnA to F) for ribosomal RNA synthesis. Transcription from rrnD occurs from four promoters (p1 to p4). We found that transcripts from the p1 and p3 promoters were most abundant in vivo in the early exponential phase. However, at later phases of exponential and stationary growth, transcripts from the p1 promoter decreased drastically, with the p3 and p4 transcripts constituting the major forms. Partially purified RNA polymerase supported transcription from the p3 and p4 promoters, whereas pure reconstituted RNA polymerase with core enzyme (E) and the major vegetative sigma factor ${\sigma}^{HrdB}$ ($E{\cdot}{\sigma}^{HrdB}$) did not. In order to assess any potential requirement for additional factor(s) that allow transcription from the p3 and p4 promoters, we fractionated a partially purified RNA polymerase preparation by denaturing gel filtration chromatography. We found that transcription from the p3 and p4 promoters required factor(s) of about 30-35 kDa in addition to RNAP holoenzyme ($E{\cdot}{\sigma}^{HrdB}$). Therefore, transcription from the p3 and p4 promoters, which contain a consensus -10 region but no -35 for ${\sigma}^{HrdB}$ recognition, are likely to be regulated by transcription factor(s) that modulate RNA polymerase holoenzyme activity in S. coelicolor.

• Journal of Korean Academy of Nursing
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• v.19 no.3
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• pp.273-284
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• 1989

The aim of this investigation was to delineate concepts contributing to the Exercise of Self-Care Agency (E.S.C.A.) Scale developed by Kearney & Fleischer (1979) and to test its construct validity and its predictors. The results are summarized as follows ; 1. By means of principal factor analysis and maximal likelihood factor analysis upon data generated from 280 undergraduate students, the factors of Concern about Health Knowledge, self-Concept, Information - Seeking Behavior, Decision -making and Responsibility, Self-esteem, and Passivity emerged. The total percent of variance explained by the 6 factors was 75.1% 2. To assess factor independence and instrument homogeneity, correlations among the 6 factors were computed. The correlations ranged from .24 to .46 indicating that the factors and the items were not too similar or redundant. 3. Test-retest reliability of the total scale is r＝.70. Cronbach's $\alpha$ coefficient for internal consistency of the total scale is .86 and that of the factors ranged from .750 to .661 (only factor 6 .497). 4. In additional analysis of the Exercise of Self-Care Agency Scale in relationship to Multiple Health Locus of Control, Family Environmental Scale, and Cornell Medical Index using stepwise multiple regression, the Internal Health Locus of Control Score predicted 21.8%(F＝53.34, p＝.0001), Family Environmental Score 8.3%(F＝22.59, P＝.0001), Modified Cornell Medical Index Score 5.4%(F＝15.74, P＝.0001) of the score of the E.S.C.A.

• Structural Engineering and Mechanics
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• v.4 no.2
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• pp.139-148
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• 1996

Vector algorithms and the relative importance of the four basic modules (computation of element stiffness matrices, assembly of the global stiffness matrix, solution of the system of linear simultaneous equations, and calculation of stresses and strains) of a finite element computer program for inelastic analysis of reinforced concrete shells are presented. Performance of the vector program is compared with a scalar program. For a cooling tower problem, the speedup factor from the scalar to the vector program is 34 for the element stiffness matrices calculation, 25.3 for the assembly of global stiffness matrix, 27.5 for the equation solver, and 37.8 for stresses, strains and nodal forces computations on a Gray Y-MP. The overall speedup factor is 30.9. When the equation solver alone is vectorized, which is computationally the most intensive part of a finite element program, a speedup factor of only 1.9 is achieved. When the rest of the program is also vectorized, a large additional speedup factor of 15.9 is attained. Therefore, it is very important that all the modules in a nonlinear program are vectorized to gain the full potential of the supercomputers. The vector finite element computer program for inelastic analysis of RC shells with layered elements developed in the present study enabled us to perform mesh convergence studies. The vector program can be used for studying the ultimate behavior of RC shells and used as a design tool.

• Proceedings of the Korean Geotechical Society Conference
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• 2005.03a
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• pp.677-686
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• 2005

The allowable bearing capacity of a pile, the most important factor in stability estimation, is determined by applying safety factor to the ultimate load or yield load. There are several but contradictory methods available in current design codes to estimate the allowable bearing capacity and the safety factor. This paper analyzes load-settlement curves obtained from 19 static load tests measured from 11 sites. At all tests, the load is applied until apparent failure is observed. The validity of the ultimate and yield load estimation method and load caculated from the settlement criterion is investigated through comparison with the measured data. In addition, a new procedure to estimate allowable load and safety factor is proposed. Additional data from field static load tests, such as those incorporated in this study, are needed to more reliably apply the proposed method in design practice.

• Tribology and Lubricants
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• v.17 no.3
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• pp.236-243
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• 2001

In this study, flow factors are evaluated in terms of kurtosis using random rough surface generated numerically. As h/$\sigma$become large ø$\sub$x/, ø$\sub$y/, ø$\sub$fp/, approach to 1 and ø$\sub$s/, ø$\sub$fs/ to 0 asymptotically regardless of kurtosis. ø$\sub$x/, ø$\sub$y/, ø$\sub$fp/ increase with increasing kurtosis in the mixed lubrication regime. ø$\sub$s/, ø$\sub$fs/ is associated with an additional flow transport due to the combined effect of sliding and roughness. As h/$\sigma$ decreases ø$\sub$s/, ø$\sub$fs/ increase up to a certain point, and then decrease toward zero. This behavior can be attributed to the increasing number of contacts in the mixed lubrication regime. ø$\sub$x/ in the presence of elastic deformation on the surface is larger than ø$\sub$x/ in the absence of it because local film thickness(h$\sub$T/) increases by elastic deformation.

• Korea Journal of Hospital Management
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• v.15 no.4
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• pp.125-142
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• 2010

Objective : To determine demographic, clinical, health care utilization factors predicting unplanned readmission(within 28 days) to the hospital. Methods : A case-control study was conducted from January to December 2009. Multiple logistic regression was used to examine risk factors for readmission. 180 patients who had been readmitted within 28 days and 1,784 controls were recruited from an university hospital in Seoul. Results : Six risk factors associated with readmission risk were identified and include mail sex, medical service rather than surgical service, number of comorbid diseases, type of patient's room, lenth of stay, number of admissions in the prior 12 months. Conclusions : One of the association with readmission risk identified was the number of hospital admissions in the previous year. This factor may be the only risk factor necessary for assessing prior risk and has the additional advantage of being easily accessible from computerized medical records without requiring other medical record review. This risk factor may be useful in identifying a group at high readmission risk, which could be targeted in intervention studies. Multiple risk factors intervention approach should be considered in designing future prevention strategies.

• Tunnel and Underground Space
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• v.30 no.1
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• pp.63-75
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• 2020

When tunnel is excavated via drilling and blasting, the excessive overbreak is the primary cause of personal or equipment safety hazards and increasing the cost of the tunnel operation owing to additional ground supports such as shotcrete. The practical management of overbreak is extremely difficult due to the complex causative mechanism of it. The study examines the relationship between rock mass characteristics (unsupported face condition, uniaxial compressive strength, face weathering and alteration, discontinuities- frequency, condition and angle between discontinuities and tunnel contour) and the depth of overbreak through using feed-forward artificial neuron networks. Then, Overbreak Resistance Factor (ORF) has been developed based on the weights of rock mass parameters to the overbreak phenomenon. Also, a new concept of tunnel overbreak management system using ORF has been suggested.