• 대한약침학회지
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• 제5권2호
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• pp.120-136
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• 2002

Objectives : The purpose of this study is to investigate Acute and Subacute Toxicity, and Anti-cancer Effect of H Herbal-acupuncture on mice and rats. Methods : Balb/c mice were injected intraperitoneally with H Herbal-acupuncture for $LD_{50}$ and acute toxicity test. Sprague-Dawley rats were experimented in the same way for subacute toxicity test. H Herbal-acupuncture was injected into abdomen of mice having S-180 cancer cell line. Result : 1. During the test, $LD_{50}$ could not be counted since there was no expired subjects. 2. In an acute toxicity test, the loss of motility and reflex action was observed, but weight increased in the treatment group, compared with those in the normal group (P<0.05). 3. In an acute toxicity test of serum biochemical values of mice, glucose increased in the treatment group II while total cholesterol was increased in the all treatment groups (P<0.05). 4. In a subacute toxicity test, a little loss of motility and reflex action was observed in the treatment group. Weight of mice in the treatment group decreased on the 28th day. 5. In a subacute toxicity test, liver weight was decreased but lung weight of mice increased in the all treatment groups (P<0.05). 6. As a result of measuring Complete Blood Count test (CBC) of rat, HCT was decreased in treatments even though it was not significant, compared with the normal group (P<0.05). 7. In a serum biochemical value test of subacute toxicity, total protein and albumin decreased in the all treatment groups. Creatinine, glucose, GOT increased in the treatment group I compared with the control group. Alkaline phos-phatase decreased in treatment II group, compared with the control group (P<0.05). 8. Median survival time that was measured in the rats treated with sarcoma-180 cancer cell Median decreased in the treatment group, compared with the control group (P<0.05). 9. Natural killer cell activity showed significant reduction at 100:1 and 10:1 E/T ratio while it increased at 50:1 E/T ratio. It is inferred that there was an error in the experiment (P<0.05). 10. In an interleukin-2 productivity test, even though it decreased in lung cancer, and increased in abdomen cancer, but it was only a small difference (P<0.005). 11. After injecting B16F10 cell into a capillary vessel of C57BL/6 mice and generating metastasized lung cancer, the lung was examined with the naked eye. It was not possible to see metastasized cancer in the all groups on the seventh day but the cancer was viewed on the fourteenth day. The number and volume of metastasized cancer in the treatment group enlarged in the treatment group, compared with the control group. Conclusion : According to the results, H herbal-acupuncture took no effects in cancer.

• 한국물환경학회지
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• 제31권1호
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• pp.49-54
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• 2015

Acute toxicity test with Daphnia magna has a variety of factors such as incubation condition of test species, food quality and proficiency of laboratory workers. Therefore, proper proficiency testing samples were required for evaluating the competence of laboratory workers. This study aims to propose some appropriate proficiency testing samples for laboratory workers of acute toxicity test with Daphnia magna. Proposed four kinds of standard samples (Sample A, B, C, and D) for proficiency testing were selected from reference materials which were recommended from Canada and USA WET(Whole Effluent Toxicity) authorities. Proposed standard samples were tested by trial application of proficiency testing, homogeneity and stability checks using repeatability test. Sample A, C and D were estimated ad the suitable candidates for proficiency test standard samples except for Sample B. In addition, Sample A was proved to be the most suitable sample.

• 대한약침학회지
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• 제5권1호
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• pp.7-26
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• 2002

Objective: The purpose of this study was to investigate acute and subacute toxicity and sarcoma-180 anti-cancer effects of herbal acupuncture with Carthami- Tinctorii fructus (CF) in mice and rats. Method: Balb/c mice were injected intraperitoneally with Carthami - Tinctorii fructus (CF) for $LD_{50}$ and acute toxicity test. Sprague Dawley rats were injected intraperitoneally with Carthami- Tinctorii fructus (CF) for subacute toxicity test. The Carthami- Tinctorii fructus herbal-acupuncture was injected on Chung-wan (CV12) of mice with Sarcoma-180 cancer cell line. Results: 1. $LD_{50}$ was uncountable as none of the subjects expired during the test. 2. In acute toxicity test, toxic symptoms were not detected, but the body weight of mice was increased in treatment Ⅰ, treatment Ⅱ groups, compared to the normal group.(p<0.05) 3. In acute toxicity test of serum biochemical values of mice, glucose was increased in treatment Ⅰ and treatment Ⅱ groups, total cholesterol was increased in treatment I group, GOT was decreased in treatment Ⅱ group, and GPT was decreased in treatment Ⅰ group, compared to the normal group.(p<0.05) 4. The clinical signs and the body weight of mice treated with 0.1 cc, 0.2cc Carthami- Tinctorii fructus (CF) were not affected during the subacute toxicity test. 5. In subacute toxicity test, treatment groups didn't show significant changes in complete blood count test (CBC) of rats, compared to the nonnal group.(p<0.05) 6. In subacute toxicity test of serum biochemical values of rats, uric acid was decreased in treatment Ⅰ and treatment Ⅱ groups, compared to the nonnal group, triglyceride was decreased in treatment I group, compared to the normal group, GOT and GPT were decreased in treatment I and treatment Ⅱ groups, and alkaline phosphatase was decreased in treatment Ⅰ and treatment Ⅱ groups, compared to the normal group.(p<0.05) 7. Median survival time was increased in all the treatment groups for Sarcoma-180 cancer cell treated with Carthmni- Tinctorii fructus (CF).(p<0.05) 8. Natural killer cell activity was significantly increased in all the treatment groups compared to the normal group.(p<0.05) 9. Interleukin-2 productivity was decreased in treatment Ⅰ and treatment Ⅱ groups.(p<0.05) Conclusion: According to the results, we can conclude herbal-acupuncture of Carthami-Tinctoriifructus (CF) caused negligible toxkity, and had anti-tumor effects in mice.

• Journal of Acupuncture Research
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• 제15권2호
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• pp.357-367
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• 1998

This experiment was carried out to study on the acute toxicity of Thesium Chinese Turczaninow solution for aqua-acupuncture. Test material was manufactured by 'Water Alcohol method'. Mice and rats were used for this test. According to this experimental results, there were not observed any acute toxicity with Thesium Chinese Turczaninow solution for aqua-acupuncture. So Thesium Chinese Turczaninow solition for aqua-acupuncture is considered a have level of cliniccal safety. It is expected to be clinically helpful, and it is considered that continuous study is necessary.

• 한국수산과학회지
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• 제37권3호
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• pp.209-214
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• 2004

A method for rapid acute toxicity test based on temperature control of Ceriodaphnia dubia has been developed and evaluated. A new toxicity test based on temperature control (TTBTC) which are based on temperature control, was developed and compared for the adsorption of the better methodology for short-term toxicity screening. Initially, daphnid larval are exposed to toxicants and at the same time the temperature of the water bath containing media is increased to high temperature $(35.5^{\circ}C).$ After 1.25 hrs of contact time, the number of the daphnids, either living (no toxic effect) or dead (toxic effect), is counted by the naked eyes. Effect of exposure time on test sensitivity was not significantly different between 1 to 1.5 hr. Comparison of the rapid 1.25 hr acute toxicity test developed in this study and the standard 48 hr acute toxicity test using heavy metals, cyanide and pentachlorophenol indicated that the 1.25 hour test provides an acceptable level of sensitivity in toxicity test for C. dubia.

• 농약과학회지
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• 제8권4호
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• pp.309-318
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• 2004

우리나라에서 널리 사용되고 있는 벼재배용 살충제인 carbosulfan과 phenthoate의 담수어에 미치는 급 만성적 영향을 알아보고자 송사리(Oryzias latipes, Medaka)에 대한 급성독성시험(Acute Toxicity Test)과 생육초기 독성시험(Early-life Stage Toxicity Test)을 실시하였다. 급성독성시험 결과 carbosulfan과 phenthoate의 송사리에 대한 96시간 $LC_{50}$은 0.102, 0.167 mg/L로 비슷한 감수성을 보였다. 생육초기 독성시험 결과 carbosulfan의 경우 부화율은 80%이상, 부화기간은 $9\sim10$일로 나타나 처리구간 유의성이 없었다. 처리농도 0.042 mg/L이상에서는 부화후 생존율이 통계적으로 유의한(p<0.05) 차이를 보였으며, 척추가 휘어지는 형태적으로 비정상적인 결과가 관찰되었다. 시험 종료후 생존한 송사리의 전장 및 체중 변화는 농도 상관성을 보였으며, 특히 0.017 mg/L이상에서는 대조구에 비해 유의한(p<0.05) 감소를 나타내었다. 따라서 이런 결과를 근거로 한 무영향농도(NOEC), 최저영향농도(LOEC), 그리고 최대허용농도(MATC)는 각각 0.0067, 0.017, 0.011 mg/L으로 산출하였다. phenthoate 처리구의 부화율과 부화기간은 처리 농도간 유의성이 없었으나, 부화후 생존율은 처리농도 0.076 mg/L 이상에서 유의한(p<0.05) 차이를 보였다. 또한 처리농도 0.076 mg/L 이상에서는 척추기형과 유영이상을 보이는 이상어가 나타났다. 그러나 시험 종료후 송사리 전장 및 체중의 변화는 통계적인 유의한 차이가 없었으며, 농도 상관성도 보이지 않았다. 따라서 이런 결과를 종합하여 NOEC, LOEC, 그리고 MATC는 각각 0.011, 0.029, 0.018 mg/L으로 결정되었다.

• 생약학회지
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• 제40권3호
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• pp.251-256
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• 2009

Cordyceps bassiana is a parasitic fungus and used as a Chinese traditional medicine. It has been called as DongChungHaCho(summer-plant, winter-worm) in China. Acute oral toxicity was examined in male and female ICR mice. Butanol fraction from Cordyceps bassiana(BuCb) was administered orally at a dose of 2,500 mg/kg, 5,000 mg/kg, 10,000 mg/kg. No death and abnormal clinical signs were observed throughout the administration period. The acute toxicity test on mouse did not show any oversign in net body weight gain, food and water consumptions, organ weights, gross pathological findings by different doses of BuCb. Also, biochemical examination revealed no evidence of specific toxicity. These findings show that BuCb has wide margin of safety on acute toxicity with single exposure.

• 대한약침학회지
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• 제5권2호
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• pp.6-24
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• 2002

This experiment was carried out to study on the safety assessment of Bos tures$^{\circ}{\S}Ursus thibetanus^{\circ}{\S}$Moschus extract solution(BUM) for Herbal-acupuncture. SD rats and ICR mice were used for acute toxicity test. the results were summerized as follows; 1. In rats and mice, LD50 value could not be measured. 2. There were no abnormal finding in acute toxicity test treated BUM for Herbal-acupuncture

• 한국물환경학회지
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• 제31권5호
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• pp.453-459
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• 2015

This paper aims to evaluate the results of acute toxicity testing with Daphnia mag$Na^+$ and Vibrio fischeri and characteristics of ionic substance of treated effluent which contained salt. Acute toxicity with Daphnia mag$Na^+$ and Vibrio fischeri and salinity of 19 samples (4 business categories) were a$Na^+$lysed. Salinity of effluent could explain the fluctuation of toxicity with D. mag$Na^+$ about 66% ~ 91% ($r^2=0.66{\sim}0.91$). The results of acute toxicity testing with V. fischeri of treated effluent (aggregate manufacture facilities) did not indicate toxicity (TU = 0), whereas that of chemical manufacture facilities indicated toxicity. V. fischeri, a candidate test organism, seemed suitable test organism for acute toxicity testing of effluent except high salinity (above 65‰ ~ 70‰) in aggregate manufacture facilities (nonmetalic minerals facilities). The performance of ion composition about treated effluent of surveyed facilities indicated that ion concentration of $Na^+$ (5,740 mg/L) and $Cl^-$ (9,727 mg/L) showed high level among 6 major ions ($Na^+$, $K^+$, $Ca^{2+}$, $Mg^{2+}$, $SO_4{^{2-}}$, $Cl^-$) in effluent of nonmetalic minerals facilities. In addition, Clion seemed to influence the D. magna survival rather than $Na^+$ ion.

• 한국유기농업학회지
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• 제30권1호
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• pp.103-118
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• 2022

Photorhabdus is a bacterial symbiont of entomopathogenic nematodes of the genus Heterorhabditis in the family Heterorhabditidae. Photorhabdus is known to have nematicidal activity in addition to insecticidal activity. P. temperata isolated from Korean indigenous H. megidis Gwangju strain also produced high control efficacy against root-knot nematode Meloidogyne incognita and root-lesion nematode Pratylenchus penetrans. P. temperata has drawn interest as a potential bionematicide for the control of root-knot nematodes thereby. For the registration as an organic agricultural material, the toxicity of P. temperata was assessed by the acute toxicity test in carp (Cyprinus carpio) and acute oral and dermal toxicity tests in Sprague-Dawley rat (Rattus norvegicus) in compliance with the guidelines of the Rural Development Administration (RDA). In the acute toxicity test in fish, neither lethality nor abnormal responses of carp were observed. Body length and weight of carp and changes in DO concentrations and pH values were not significantly different between the treated group and the untreated control. In the acute oral and dermal toxicity tests, clinical signs, abnormal behavior, mortality, and pathological findings were not observed in all the experimental rats. The weight increment of all rats was normal. Acute toxicity results of P. temperata in fish and rats belonged to categories III, IV, and IV of RDA, respectively. Toxicity results of the present study indicated that P. temperata could be a safe and promising bionematicide against root-knot nematodes and root lesion nematode.