• Biomolecules & Therapeutics
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• 제2권2호
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• pp.161-172
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• 1994

This study was conducted to examine DA-3002, a biosynthetic human growth hormone, for its acute and subacute toxicities in mice and rats. The drug was administered subcutaneously and orally at a dose level of 1.0, 3.0, 8.9, 26.7 or 80.0 lU/kg once for single dose toxicity and given subcutaneously at a dose level of 0.34, 1.7 or 8.4 lU/kg daily for 13 weeks to investigate repeated dose toxicity. In the acute toxicity study, doses up to 80 lU/kg had no adverse effect on the behavior or body weight gain. Pathological examinations revealed no abnormal changes which could be attributed to toxic effect of DA-3002. In the subacute toxicity study, the growth hormone was tolerated well in broth mice and rats. No drug related deaths occurred and all animals appeared to be normal throughout the dosing period. Increases in body weight gain, food utilisation and absolute organ weights were observed in the rats in the high dose group. Mild changes in the blood chemical parameters were also seen in the treated groups. Histopathologically, however, no abnormal changes were observed in any organ. The changes noted during the treatment periods presumably represent exaggerated pharmacological effects of the growth hormone, and no observed adverse effect level (NOAEL) was considered to be more than 8.4 lu/kg/day.

• Toxicological Research
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• 제13권3호
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• pp.183-186
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• 1997

Acute toxicity of pectenotoxin 2 (PTX2) was examined in mice. Treatment of mice with a toxic dose of PTX2 resulted in clinical signs such as ataxia, cyanosis and an abrupt decrease in body temperature. Histopathological studies revealed that the liver is the major target organ for PTX2. Activities of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) and sorbitol dehydrogenase (SDH) were significantly elevated by PTX2 administration. Glucose-6-phosphatase activities were not changed by the treatment. The PTX2 treatment decreased relative liver weight without changing the body weight. The effect of PTX2 on hepatic drug metabolizing enzyme system was determined. An ip dose of PTX2 (200 $\mu$g/kg) induced a significant decrease in the hepatic microsomal protein content. Cytochrome P-450 content, cytochrome b$_5$ content, NADPH cytochrome c reductase, aminopyrine N-demethylase activities, or hepatic glutathione content were not altered by PTX2 treatment.

• Toxicological Research
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• 제5권2호
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• pp.105-109
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• 1989

Single dose of Hantabax, HFRS-vaccine, was given to both sexes of Sprague-Dawley rats and ICR mice, subcutaneously and intraperitoneally. Any toxic symptom was not noted in the treated animals. Macroscopic examination on the organs of tested animals showed no abnormal findings. In general toxicological aspects Hantabax was practically nontoxic in rats and mice upto a single dose of 1000 times of human clinical dose equivalent via subcutaneous and intraperitoneal administration.

• 약학회지
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• 제29권1호
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• pp.1-10
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• 1985

The ether fraction obtained from dry fruit of Cornus officinalis was investigated for the anti-inflammatory action, acute toxicity and central nervous system activities. From several pharmacological examinations, it was found that the ether fraction suppressed considerably carrageenin edema at the dose of 600mg/kg p.o. in rats, inhibited the granuloma formation in rats as given 100mg/kg p.o. for 7 days and decreased the swelling of both of complete adjuvant injected and noninjected (contralateral) paws of the rats at the dose of 100mg/kg p.o. given for 14 days. The $LD_{50}$ of the fraction are estimated to be more than 2,000mg/kg p. o. and 642mg/kg i. p. in mice. And the fraction did not show any sedative, stimulative, analgesic and anticonvulsant action but exhibited hypothermic action. These results might be concluded that the ether fraction of Corni fructus showed anti-inflammatory action in both of acute and chronic type models without any considerable central nervous depressant activity and exhibited very weak acute toxicity in mice.

• 생약학회지
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• 제33권1호통권128호
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• pp.5-12
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• 2002

This study was performed to evaluate hepatoprotective effect of Daewhang-whangryunhaedok-Tang(DWT) on liver injured rats induced by $CCl_4$ and the acute oral toxicity of it in mice. The activities of serum transaminase(ALT/AST), alkaline phosphatase(ALP) and lactic dehydrogenase (LDH), the levels of serum total cholesterol(TC) and triglyceride(TG), change of liver enlargement, and inhibitory activities of lipid peroxidation, catalase and glutathione-S-transferase(GST) in liver microsome were determined in hepatotoxic rats induced by $CCl_4$ DWT DWT was significantly reduced the serum ALT, AST, ALP, LDH, TC and TG levels. And, the increase of lipid peroxidation, decrease of catalase and GST activities in the liver microsome of $CCl_4$-intoxicated rat were significantly improved by the treatment of DWT. Male and female mice were administered maximum dosages of 5,000 mg/kg b.w. of DWT. After single oral administration of DWT to mice, we observed them daily for 2 weeks. DWT did not induce any toxic signs in the mortalities, clinical signs, body weight changes, and gross necropsy findings of mice. Based on these results, it is concluded that DWT may have the hepatoprotective effect on $CCl_4$ induced hepatotoxicity in rats. Also, DWT may have no side effect and its $LD_{50}$ value may be over 5,000 mg/kg b.w. in mice.

• 동의생리병리학회지
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• 제20권3호
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• pp.572-574
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• 2006

The Dangguibohyel-tang (DBT) was used to treat anemia in traditional Korean medicine. Acute oral toxicity of DBT was studied in ICR mice. ICR mice were administered orally with dosages of 100 mg/kg (low dosage group), 200 mg/kg (middle dosage group), and 400 mg/kg (high dosage group) of DBT. We daily examined number of deaths, clinical signs, body weights and gross findings for 14 days. DBT did not show any toxic effect in ICR mice and oral LD50 value was over 400 mg/kg in ICR mice.

• 한국식품위생안전성학회지
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• 제8권2호
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• pp.97-103
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• 1993

랫드와 마우스에서 고흡수성 중합체인 폴리(아크릴산소다-아크릴산)의 급성경구 독성시험을 수행하였다. 본 시험물질을 최고 10 g/kg까지 corn oil에 현탁하여 1회 경구투여하고 2주간 관찰한 결과, 사망사례가 없었으며 일반적인 임상증상 관찰시 특기할 만함 독성증상이 나타나지 않았고, 체중변화도 대조군과 유의성있는 차이를 보이지 않았다. 또한 부검후 각 장기의 육안적 관찰 결과도 이상소견이 없었다. 따라서 본 실험에 사용한 폴리(아크릴산소다-아크릴산)은 $LD_{50}$가 10 g/kg 이상일 것으로 사료되며, 10 g/kg까지 투여하였을 때 랫드와 마우스에서 특이한 독성이 관찰되지 않았다.

• 대한한방내과학회지
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• 제32권4호
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• pp.599-609
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• 2011

Objectives : The aim of this study was to evaluate the single oral dose toxicity and safety of Bojungikgi-tang (Buzhongyiqi-tang) and fermented Bojungikgi-tang (Buzhongyiqi-tang) extracts in male and female ICR mice. Methods : In the single oral dose toxicity study, non-fermented and fermented Bojungikgi-tang (Buzhongyiqi-tang) were administered to male and female ICR mice as an oral dose of 1250, 2500 and 5000 mg/kg. Changes of body weights, general behaviors, adverse effects and mortality were determined throughout the experimental period. Hematological parameters, serum chemistry, organ weights and necropsy findings were evaluated at the end of the experiment. Results : There was no mortality or sign of toxicity in the single oral dose toxicity study. There were also no significant differences in body weights, organ weights, and hematological parameters, serum chemistry values between treatment and control groups. Conclusions : The results obtained in this study suggest that the 50% lethal dose of fermented Bojungikgi-tang (Buzhongyiqi -tang) in both female and male mice can be considered as well over 5,000 mg/kg, so these medicines can be safe in clinics.

• Journal of Ginseng Research
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• 제13권1호
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• pp.5-7
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• 1989

The objective of this study was to investigate the preventive effect of ginseng on acetaldehyde-induced acute toxicity in mice . Compared to the control group, treatment with acetaldehyde inhibited the hepatic cytosolic xanthine oxidise activity with increase in dose. The inhibition of enzyme activity was not changed after dialysis. Pretreatment with ginseng butanol fraction prevented the inhibition of enzyme activity by acetaldehyde. In conjunction with the our previous results (Yakhak Hoeji, 29, 18 (1985)), these results suggest that the most likely mechanism for the observed preventive effects of ginseng against the acetaldehyde-induced acute toxicity may be the decrease hepatic acetaldehyde level.

• 생약학회지
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• 제19권4호
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• pp.262-269
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• 1988

The Forsythiae fructus is described to be used as an antiinflammatory drug, diuretics, antidotes and antibacterials in oriental literatures. In order to investigate the efficacy of Forsythiae viridissima (Oleaceae), the methanol extract and its fraction have been evaluated for the acute toxicity, antiinflammatory, analgesic and spasmolytic action in animals. The methanol extract of Forsythiae fructus was found to have significant antiinflammatory activity in the acute and subacute antiinflammatory model in rats, but have no analgesic action. Furthermore, through fractionation procedure, it was found that the active compounds were easily soluble in chloroform and butanol. It is also noted that the extracts had spasmolytic activities in the rat fundus and uterus and had low acute toxicity in mice.