• 한국수산과학회지
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• 제37권2호
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• pp.116-121
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• 2004

Physiological responses and tolerance limit of hybrid striped bass $(Morone\;Chrysops\;male{\times}\;M.\;saxatilis\;female),$ were investigated on salinity changes from 33 psu to 0 psu and from 0 to psu 33 psu in two separate trials, respectively. In the acute salinity decrease from sea water (33 psu) to fresh water (0 psu), plasma level of cortisol, glucose, total protein, aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were not affected, however, $Na^+,\;Cl^-$ and osmolality significantly decreased. In the acute salinity increase from fresh water to sea water (33 psu), plasma cortisol level did not change. Glucose and total protein concentrations in plasma and hematocrit (Hct) decreased, whereas AST, ALT, $Na^+,\;Cl^-$ and osmolality increased. The hybrid striped bass can overcome the acute changes of salinity, from 0 psu to 33 psu or from 33 psu to 0 psu, But salinity decrease stressed the fish much more than salinity increase.

• 한국발생생물학회지:발생과생식
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• 제20권3호
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• pp.187-196
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• 2016

The objective of the current study was to determine acute plasma stress responses in two size groups of juvenile Epinephelus akaara (average body weight: $8.4{\pm}2.1$ and $3.3{\pm}0.6g$; 150 and 120 days after hatch, respectively) exposed to abrupt salinity drops (from 34 practical salinity unit, PSU seawater to 18, 10 PSU (experiment 1) or 26, 18, 10 PSU (experiment 2), respectively). Plasma glucose, glutamic oxalate transaminase, glutamic pyruvate transaminase, red blood cell counts, and gill histology were determined during 72 h exposure. Significantly increased plasma glucose, glutamic oxalate transaminase levels, and red blood cell counts were observed in fish exposed to 18 or 10 PSU. Histological changes, such as hyperplasia and lifting of epithelium in the gill secondary lamellae, were also observed in fish exposed to 18 or 10 PSU at 72 h post-drop. E. akaara exposed to sudden salinity drops to 18 or 10 PSU still seems to undergo the primary adjustment phase before fish reaches a new homeostasis, whereas fish exposed to 26 PSU seems to mount osmotic changes. Therefore, the no observed adverse effect levels for 72 h acute salinity challenge was 26 PSU in our study, and salinity drop to 18 PSU and below can possibly cause acute adverse effect, in which fish could be vulnerable to additional stresses such as a temperature changes or handling stress.

• 한국양식학회지
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• 제16권1호
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• pp.31-36
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• 2003

사육 염분의 급성교환 실험에서, 해수의 담수화가 1시간 이내에 이루어졌을 경우 농어, 유어들은 다소의 스트레스 반응과 이온의 변화가 있었으며. 24시간 뒤에도 정상 회복이 더딘 것으로 나타났다. 그러나 5시간에 걸친 담수화 과정 중에 스트레스 반응은 담수화 직후 높게 나타났지만, 24시간 후 빠르게 정상으로 회복되는 것을 알 수 있다. 장기간 염분별 사육실험 결과, 20 ppt가 가장 높은 사료섭식량을 나타내었고, 2 ppt구가 가장 낮았다. 사료효율 또한 2 ppt구이 가장 낮게 나타났고, 10 ppt구가 가장 높았으며, 실험구별 유어의 성장은 실험 종료시 10, 20 및 30 ppt구가 2 ppt보다 유의하게 높은 길이 성장을 보였다. 무게 성장은 실험 종료시 20 ppt가 가장 빠른 무게 성장을 나타내었고, 다음으로 30 ppt, 10 ppt. 2 ppt순으로 나타났다. 염분별 사육유어의 혈액 성상은 2 ppt~30 ppt 사육수에 있어 농어 유어들의 혈액 조성은 큰 차이가 없는 것으로 나타났다.

• 한국물환경학회지
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• 제31권5호
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• pp.453-459
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• 2015

This paper aims to evaluate the results of acute toxicity testing with Daphnia mag$Na^+$ and Vibrio fischeri and characteristics of ionic substance of treated effluent which contained salt. Acute toxicity with Daphnia mag$Na^+$ and Vibrio fischeri and salinity of 19 samples (4 business categories) were a$Na^+$lysed. Salinity of effluent could explain the fluctuation of toxicity with D. mag$Na^+$ about 66% ~ 91% ($r^2=0.66{\sim}0.91$). The results of acute toxicity testing with V. fischeri of treated effluent (aggregate manufacture facilities) did not indicate toxicity (TU = 0), whereas that of chemical manufacture facilities indicated toxicity. V. fischeri, a candidate test organism, seemed suitable test organism for acute toxicity testing of effluent except high salinity (above 65‰ ~ 70‰) in aggregate manufacture facilities (nonmetalic minerals facilities). The performance of ion composition about treated effluent of surveyed facilities indicated that ion concentration of $Na^+$ (5,740 mg/L) and $Cl^-$ (9,727 mg/L) showed high level among 6 major ions ($Na^+$, $K^+$, $Ca^{2+}$, $Mg^{2+}$, $SO_4{^{2-}}$, $Cl^-$) in effluent of nonmetalic minerals facilities. In addition, Clion seemed to influence the D. magna survival rather than $Na^+$ ion.

• 환경영향평가
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• 제21권5호
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• pp.707-713
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• 2012

There are a lot of parameters affecting microbial acclimation/cultivation characteristics such as dynamic conditions, F/M ratio and substrate affinity. From the process control point of view, the effect of high salinity on the removal efficiencies of BOD and SS have been documented by few researchers. In this research, lab-scale CAS(Conventional Activated Sludge) process and modified $A_2O$(Anaerobic/Anoxic/Oxic) process were operated and monitored to evaluate the characteristics of microbial acclimation and cultivation under high salinity wastewater during the period of three weeks. As a result of acute microbial activity test(6hr) at various $Cl^-$ concentration, the appropriate $Cl^-$ concentration for microbial growth and acclimation ranged under 3,100 mg/l. As a result of acclimation/cultivation test, the trend of COD removal efficiency reduced gradually as time elapsed. It is considered that $NH_4$-N removal phenomenon of the conventional pollutants removal mechanisms gave little effect to the microbial acclimation/cultivation under high salinity wastewater.

• 한국발생생물학회지:발생과생식
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• 제23권3호
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• pp.239-253
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• 2019

We investigated the physiological responses of the river puffer, Takifugu obscurus, the tiger puffer, T. rubripes, a hybrids of the two (river puffer${\times}$tiger puffer) and a hybrid triploids to acute changes of salinity from 30 psu to 0 psu and from 0 psu to 30 psu. The blood and plasma factors of each species were elevated for 48, 72, or 96 hrs, and thereafter decreased due to hyper-osmoregulation and hypo-osmoregulation. In hyper-osmoregulation and hypo-osmoregulation, the cortisol concentration of river puffer, hybrids, hybrid triploids and tiger puffer increased for 12 or 48 hrs, and decreased thereafter. Chloride cells in the gill filaments of each species increased with increasing salinity, and melano-macrophages in the kidney tissue of each species increased with decreasing salinity. In conclusion, the hematological and stress responses of the hybrids were between those of the river puffer and tiger puffer, and the hematological responses of the hybrid triploids were higher than those of the other groups. The stress response of the hybrids was more sensitive than that of the hybrid triploids. In all groups, the histological responses of kidney in hyper-osmoregulation were more sensitive than those in hypo-osmoregulation.

• 한국어류학회지
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• 제22권1호
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• pp.9-16
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• 2010

본 연구에서는 광염성 송사리 Oryzias dancena 성체 및 자어의 염분도 변화에 대한 내성 및 생존 능력을 평가하고자 다양한 염분도 변화 처리를 수행하였다. 담수로부터 고 염분도 조건으로의 급격한 염분도 증가 노출 처리에 대하여 O. dancena 성체는 $40^{\circ}/_{\circ\circ}$염분도 변화 폭까지 생존의 장애 없이 빠르게 적응할 수 있는 것으로 관찰되었으며, 단계적인 순치를 통할 경우 최대 $75^{\circ}/_{\circ\circ}$의 염분도 조건까지 생존이 가능하였다. 염분도의 감소 처리에서 O. dancena 성체는 $60^{\circ}/_{\circ\circ}$ 이상의 급격한 감소 폭에서도 우수한 생존율을 나타내였다. 성체와 달리 O. dancena 자어는 급격한 염분도 증가에 대한 삼투압 조절 능력 (hyper-osmoregulation)이 유의적으로 낮아 약 $15^{\circ}/_{\circ\circ}$ 이상의 염분도 증가에서는 생존에 큰 장애를 겪는 것으로 나타났다. 그러나 초기 발달 단계에 있는 자어의 염분도 변화에 대한 내성은 최초 염분도 처리 농도에 따라 염분도 변화에 대한 내성 폭이 크게 영향을 받는 것으로 관찰되었다. 초기 발달 단계에서 O. dancena 자어는 염분도 증가 보다는 염분도 감소 시 삼투압 조정 능력 (hypo-osmoregulation)이 더 높은 것으로 나타나 $40^{\circ}/_{\circ\circ}$에서 완전 담수로의 염분도 변화에서도 우수한 생존 능력을 보였다.

• 한국동물학회지
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• 제34권1호
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• pp.93-102
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• 1991

Larvae of Ilyoplax dentimerosa Shen, 1932 were reared in the laboratory at $25^{\circ}C$ and 33.3%o salinity. Four zoeal stages were recognized in the lanral development, and these are described and illustrated. The first soeae of 1. dentimerosa are distinguished from other llyoplax zoeae by having minute spicules on the rostral and dorsal carapace spines, a short dorsal carapace spine about half of the carapace length, a conspicuous antennal exopodite with a subterminal sutta and acute spines on the postero-lateral border of the fourth abdominal somite.

• 한국어류학회지
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• 제21권3호
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• pp.158-166
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• 2009

본 연구는 동갈돗돔의 양식생물학적 기초자료를 확보하고자 염분의 영향과 수온의 영향을 조사하였다. 염분의 경우 수정란의 부화와 부상률, 자어의 부상률 및 생존율, 치어의 성장과 생존에 미치는 영향을 조사하였다. 수온의 경우 일간 수온 변화 정도, 급성 저수온 충격, 완만한 수온 감소에 따른 생존, 먹이섭식 및 유영능력을 조사하였다. 염분의 영향에서 수정란의 부화율과 부상률 및 자어의 부상률과 생존율은 자연 해수 염분(32 psu)에 가까울수록 높게 나타났다. 치어의 성장과 생존의 경우에는 기수의 염분(5~20 psu)의 경우 해수 염분(25~32 psu)과 유의한 차이가 없었다. 다만 담수의 경우만 유의하게 낮았다. 수온의 영향에 관한 3가지 실험 모두에서 급성이던 만성이던 수온의 하락폭이 클수록 생존율, 먹이섭식 및 유영활동이 감소하는 것을 확인할 수 있었다. 결론적으로 본 실험을 통해 동갈돗돔은 성장과 생존은 일반 해수염분에서 가장 좋았고, 월동의 임계수온은 $8^{\circ}C$로 나타났다.

• 한국수산과학회지
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• 제42권6호
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• pp.628-632
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• 2009

In order to estimate acute toxicity of various heavy metals on mysid, Neomysis japonica, the experiments were conducted by renewal bioassay method at $20\pm0.5^{\circ}C$ and $30\pm0.3$ psu salinity. The 96 hr $LC_{50}$ of Neomysis japonica exposed to cadmium, copper, zinc, arsenic, lead and chromium were $90.4\;{\mu}g/L$, $82.3\;{\mu}g/L$, $646.1\;{\mu}g/L$, $1488.5\;{\mu}g/L$, $1758.8\;{\mu}g/L$ and $1609.6\;{\mu}g/L$, respectively, and were ranked in order of toxicity: copper > cadmium > zinc > arsenic > chromium > lead.