• Pediatric Gastroenterology, Hepatology & Nutrition
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• 제4권2호
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• pp.243-248
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• 2001

저자들은 생후 2개월부터 지속되는 만성 설사를 주소로 내원한 환아에서 면역글로불린 정량 검사상 IgA의 결핍과 함께 $IgG_2$ 및 $IgG_4$ 결핍이 동반되어 있고 영아기에 빈번한 설사와 감염을 동반한 증례를 경험하였기에 보고하는 바이다.

• 대한수의학회지
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• 제43권3호
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• pp.477-483
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• 2003

Molecular diagnostic techniques have been used to identify porcine epidemic diarrhea virus (PEDV), a causative agent of acute enteritis in swine, but they were difficult to be petformed and time-consuming. To detect PEDV in a rapid and easy way, we developed biotinylated cDNA probe for N gene encoding the nucleoproteins of PEDV. Formalin-fixed and paraffin-embedded tissues from 24 naturally infected pigs were used for the experiment. The ISH produced a positive reaction in all cases. When intestinal tissues were hybridized with PEDV probe, strong signals were seen in the villus enterocytes of the jejunum and ileum. Hybridization signals were also found in the duodenum from one pig and in colon from dnother. In conclusion, ISH with a biotinylated cDNA probe was provided to be a useful diagnostic method for detecting PEDV effectively in routinely processed tissue sections.

• 한국동물위생학회지
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• 제30권2호
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• pp.269-274
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• 2007

The aim of this study was to examine the epidemiological features of Eimeria in calves with acute diarrhea. Samples were collected from between 15 days and 90 days old calves (n=83) in Gimje area from March 2006 to March 2007. Feces of bloody diarrhea were examined for the presence of Eimeria oocysts using a sucrose flotation method. Out of 83 calves, 62 (74.6%) had Eimeria oocysts. In the results of monthly analysis, the highest prevalence (12.0%) of Eimeria oocysts was found on June. In the seasonal infection rate, spring was the highest prevalence (30.1%), followed by summer (24.0%). Furthermore, the highest prevalence (44.5%) was found in calves from between 31 - 60 days old in the analysis by ages. However, there was no significant differences between female and male sex even though the prevalence was slightly bigger in female than in male. The prevalence of the present study to detect Eimria oocysts for infection may have been affected by weather-conditions in the spring. Young calves should be separated to minimize the infection from cattle as much as possible. Additional studies are necessary to find other factors for infection and combining molecular methods with a highly sensitive system for Eimeria detection could be a reliable and economic way of Eimeria eradication.

• 한국임상수의학회지
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• 제28권3호
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• pp.287-290
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• 2011

계통발생 및 염기서열분석시 한우 송아지에서 중증의 급성 소 바이러스성 설사 바이러스 발생은 BVDV-2a에 속한 것으로 나타났고, BVDV-2 균주 890과는 독성 마커가 동일하였으며, 독성이 높은 것으로 분류되었다. 이들 BVDV 발생은 어린 송아지(20-40일령)에 주로 감염되었고, 임상증상으로 출혈성 설사, 침울, 식욕감퇴, 허약을 특징으로 하였다. 한우 송아지에서 고독성 BVDV-2의 발견은 역학조사, 진단 및 예방접종 전략에 중요한 영향을 내포한다.

• 대한미생물학회지
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• 제22권2호
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• pp.131-137
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• 1987

The incidnce of enterotoxigenic Esherichia coli(ETEC) was investigated in E. coli strains isolated from Korean infants less than two years old. Over a period of 12 months, ETEC strains have been isolated from 45(45.0%) of 100 children with acute diarrhea and from 9(20.5%) of 44 children without diarrhea. In the group with diarrhea, 41(41.0%) strains produced heat-stable toxin, 3(3.1%) produced heat-labile toxin, and 1(1.0%) produced both heat-stable and heat-labile toxins. In the control group, 7(15.9%) released heat-stable toxin, 2(4.5%) released heat-labile toxin and none released both. A statistical association of strains releasing heat-stable toxin was significant(P<0.025).

• 대한수의학회지
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• 제61권3호
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• pp.23.1-23.8
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• 2021

Porcine epidemic diarrhea (PED) is characterized by acute enteritis, watery diarrhea, weight loss, dehydration, and death with high mortality in neonatal piglets. In this study, 3 virus isolates collected in Vietnam between 2016 and 2017 were successfully propagated in Vero cells at high virus titers. Sequence analysis of the full-length spike (S) gene revealed that all 3 isolates belong to genogroup 2a, which is closely related to other prevalent Asian strains. Amino acid sequence comparisons revealed 98.19% to 99.13% homology with the Vietnam isolates circulating during 2013-2015, suggesting that field PED viruses (PEDVs) evolve continuously. Experiments in animals demonstrated that antisera from guinea pigs immunized with the vaccine strain resulted in higher levels (5 log₂) of neutralizing antibody against the homologous strain, and showed a relatively lower level of neutralizing antibody against the field isolates. This finding would be helpful in choosing a PEDV strain for vaccine development.

• 대한수의학회지
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• 제63권2호
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• pp.18.1-18.8
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• 2023

Porcine epidemic diarrhea (PED) is characterized by acute enteritis, watery diarrhea, weight loss, dehydration, and death, with high mortality in neonatal piglets. In this study, 3 virus isolates collected in Vietnam between 2016 and 2017 were propagated successfully in Vero cells at high virus titers. Sequence analysis of the full-length spike (S) gene showed that all 3 isolates belong to genogroup 2b, which is closely related to other prevalent Asian strains. A comparison of the amino acid sequence revealed a 98.19% to 99.13% homology with the Vietnam isolates circulating during 2013-2015, suggesting that field PED viruses (PEDVs) are evolving continuously. Experiments in animals showed that the antisera from guinea pigs immunized with the vaccine strain resulted in higher levels (5 log₂) of neutralizing antibodies against the homologous strain and a relatively moderate level of neutralizing antibodies against the field isolates. This finding would be helpful in selecting a PEDV strain for vaccine development.

• 대한한의학회지
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• 제25권1호
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• pp.111-116
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• 2004

Objective : To assess the clinical efficacy of Yangkyuksanwha-tang on acute stroke Methods : We prescribed Yangkyuksanwha-tang to 83 acute stroke patients without thrombolytic treatment. Results : The rate of progressive stroke type was 1.2%, it was remarkably lower than previous reports. 3.6% felt an itching sensation, 3.6% complained headache, dizziness and powerless, 2.4% complained indigestion and diarrhea, 1.2% appeared hematuria and G-I bleeding. Yangkyuksanwha-tang decreased Stroke-Pattern Identification and National Institute of Health Stroke Scale(NIHSS), and increased Modified Barthel Index(MBI). So we could suggest that this medicine have desirable effect to reduce the severity of stroke and improve functional recovery. As to the laboratory findings, all results were within the normal value, which showed no hepatic or renal toxicity. Conclusion : We could suggest that Yangkyuksanwha-tang is a useful medicine which has clinical efficacy for acute stroke, but further investigation for an administration of more than 2 weeks is necessary.

• 한국한의학연구원논문집
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• 제9권2호
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• pp.95-105
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• 2003

Kami-honghwa-tang(KH-19) is a prescription for reducing the side effect of radiotherapy. In this study, safety of KH-19 was evaluated by GLP guideline of Korea Food and Drug Administration. In acute oral toxicity study on rat, transient inhibition of weight increase was shown, but change in general symptom was not detected. No dead animal was observed up to 5,000 mg/kg in both male and female animals. In acute oral toxicity study on Beagle dog, transient vomiting, diarrhea, anorexia, and weight reduction was observed. However, no dead animal was observed up to 2,000 mg/kg in both male and female animals.

• Pediatric Infection and Vaccine
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• 제7권1호
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• pp.108-112
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• 2000

목 적 : Rotavirus와 adenovirus는 영유아에서 급성장염을 유발하는 주요 원인인데 한국에서 adenovirus에 관한 연구 논문이 적어 저자들은 최근에 발생한 adenovirus와 rotavirus 장염의 분포를 조사하여 향후 질병 예방 및 발생 정보에 도움을 얻고자 연구 분석하였다. 방 법 : 1998년 3월에서 1999년 6월까지 대상으로 건국의대 부속병원에 급성 위장관염으로 입원 또는 통원 치료를 한 143명을 대상으로 하였다. 결 과 : 전체 환아 143명중 rotavirus 양성은 53명으로 37%의 양성률을 보였으며 1999년 1월에서 3월 사이의 초봄에 가장 많이 발생하였으며 2세 이하에서 가장 많이 발생함을 보였고 2~4세 사이에서도 16%의 양성률을 보였으며 9월에 전체 24례중 6례로 가장 많이 검출되었고 2개월에서 2세 사이에 많은 발생을 보였다. Adenovirus와 rotavirus에 동시 검출된 경우는 7명(5%)이였다. 결 론 : Rotavirus 장염은 1월부터 3월에 가장 많은 발생 빈도를 보여서 겨울 및 초봄에 유행시기를 보였으며 adenovirus는 9월에 가장 많이 검출 되었다. Rotavirus와 adenovirus의 발생의 중간 나이 분포는 각각 1세 4개월, 1세 3개월의 분포를 보였다.