• Journal of the Korean Society of Food Science and Nutrition
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• v.46 no.2
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• pp.161-168
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• 2017

In this study, we examined the effect of Perilla frutescens extract (PFE) on oxidative stress-induced cell death in RGC-5 cell lines. Staurosporine-differentiated RGC-5 (ssdRGC-5) cells obtained by treating RGC-5 cells with $1{\mu}M$ staurosporine were incubated with PFE for 30 min and then exposed to buthionine sulfoximine plus glutamate (B/G) for 20 h. Cell death was detected using lactate dehydrogenase release assay and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide reduction assay. To investigate the mechanism underlying cell death, we determined caspase-3 activity, level of reactive oxygen species (ROS) formation, and expression levels of cytoplasmic cytochrome c and mitochondrial Bax. Treatment of ssdRGC-5 cells with B/G increased intracellular ROS and induced apoptosis with increasing caspase-3 activity. PFE rescued ssdRGC-5 cells from oxidative stress-induced cell death by inhibiting intracellular ROS production and caspase-3 activation and regulating apoptosis-related proteins such as cytochrome c and Bax. These findings suggest that PFE may have a beneficial neuroprotective effect against oxidative stress-induced apoptotic death in ssdRGC-5 cells.

• Korean Journal of Environmental Biology
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• v.28 no.1
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• pp.30-39
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• 2010

We researched community structure, species composition, and habitat environment of Dendropanax morbifera populations distributed in Goheung, Haenam, Wando (I.), and Bogildo (I.) at south coast of the Korean Peninsula. Our research was also estimated possible area for growth of Dendropanax morbifera by analyzing meteorological factors of the habitat thereof. Dendropanax morbifera community divided into Castanopsis cuspidata var. sieboldii group and Carpinus laxiflora group by difference of species composition. Broad-leaved evergreens showed high dominance value in view of rNCD in Dendropanax morbifera community as follows: Camellia japonica (100.0), Quercus acuta (88.6), Castanopsis cuspidata var. sieboldii (55.8), Dendropanax morbifera Lev. (41.4), Trachelospermum asiaticum var. intermedium (23.8), Eurya japonica (19.9) and Ligustrum japonicum (11.5). In the population distribution, 94.2% of Carpinus laxiflora group consisted of sapling and small size class that are less than 5 cm in DBH while 54.2% of Castanopsis cuspidata var. sieboldii group did. The soil environment of habitat showed low soil acidity, good organic matter and water content, and good drainage. The distribution area of Dendropanax morifera habitat had $13^{\circ}C$ or more in annual mean temperature, $7.4^{\circ}C$ or more in daily minimum temperature, $100^{\circ}C$ month or more in warmth index, and approximately 1,344 mm in mean annual precipitation. Dendropanax morifera habitat corresponded to evergreen broad-leaved forest zone located in islands and coast in the southwest region of the Korean Peninsula. The northern limit line of Dendropanax morifera community was determined as Gunsan-Jeongeup-Gwangju-Jinju-Pohang-Youngdeok line based on $13^{\circ}C$ in annual mean temperature that was confirmed in natural habitat of Dendropanax morifera community.

• Journal of the Korean Society of Food Science and Nutrition
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• v.37 no.11
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• pp.1491-1496
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• 2008

Twenty natural plants from Jeju were extracted by pressurized organic solvent (100% methanol, $40^{\circ}C$, 13.6 MPa, 10 min). Extraction yield, total phenolic content (TPC) and integral antioxidant capacity were measured, and each component was identified by GC/MS. Extraction yields were high as 21.8%, 21.5, 21.1, 20.7 and 20.1% in Rhus javanica, Euscaphis japonica, Alnus firma, Sapium japonicum and Sorbus alnifolia, respectively. The extracts containing high TPC (mg GAE/g of dry sample) were obtained from Malus sieboldii (68.3), Sapium japonicum (57.6), Pyrrosia lingua (56.6) and Euscaphis japonica (55.1). Integral antioxidant capacities of water-soluble substances were 598, 394, 293 and $270\;{\mu}mol$ ascorbic acid equivalent/g in Geranium thunbergii, Sapium japonicum, Cornus kousa and Rhus javanica, respectively. Integral antioxidant capacities of lipidsoluble substances were 611, 314, 296 and $242\;{\mu}mol$ trolox equivalent/g in Ardisia crenata, Ostrya japonica, Geranium thunbergii and Quercus acuta, respectively. Fifteen major peaks were identified by GC/MS from the extract of pressurized organic solvent from Sapium japonicum. Two polyphenols (gallic acid (retention time (RT) 19.7 min)) and quercetin (33.5 min)), ascorbic acid (RT 35.3 min), and several fatty acids (retention time 18.6, 21.0, 21.8, 21.9 and 23.6 min) were identified, and gallic acid was the major polyphenol component due to high peak area.

• Parasites, Hosts and Diseases
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• v.27 no.3
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• pp.203-212
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• 1989

The development of Echinestcma cinetorchis in several snail species reared in laboratory aquaria was observed. The eggs from adult cukes collected from the intestine of rats were cultivated to miracidia, and exposed to Hippeutis sp. snails. Observations were made for cercarial shedding from the exposed snails. The cercariae shed from the snails were again exposed to several species of fresh water snails in order to observe metacercarial formation in the snails and their infectivity to final hosts. The results obtained in this study were as follows: 1. Twenty miracidia were exposed to each snail of Hippeutis sp. About 58.3% of the above snails (7 out of 12) were dead before shedding the cercariae, anti the remainder shed the cercariae for a period of 7 to 9 days before death. 2. Cercarial shedding from the infected snails started from the 25th day after the exposure to mi.acidia, and the total number of cercariae shed per snail was 684 in average (range; 482-904). 3. The sixte of refine developed in the infected Hippeutis sp. snails was 1$, 242{\times}214{\;}{\mu}m$ in average, and the number of rediae per snail was 350 in average (range; 120-510). 4. About 40 to 50 cercariae shed from the Hippeutis sp. snails were each exposed to several species of snails reared in the laboratory. The metacercarial formation was confirmed by dissecting the infected snails, 12 to 16 days after the infection. The infectivity to each snail species was 100% in Hippeutis sp. (recovery rate; 56.7%) and Radix auricuzaria coreana (recovery rate; 66.4%), 66.7% in Physa acute (recovery rate; 37.5%), and 50% in Cipangopaludina sp. (recovery rate; 8.0%), respectively. 5. The swimming cercariae attached first at the cephalo-podial part of the snails and then migrated to the mantle, internal organ s and hemocele areas to form the metacercariae. 6. Adult worms of E. cinetorchis were obtained from the rats infected with the metacercariae encysted in the experimental snails. Summarising the above results, it is suggested that the mud-snail (Cipangopaludina sp.) may play an important role as a source of human infection with E. cinetorchis in Korea, and that several species of fresh water snails are involved in the life cycle as a second intermediate host.

• Korean Journal of Environment and Ecology
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• v.32 no.2
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• pp.185-202
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• 2018

Many ecological studies regarding forest succession, which is the process of arriving at climax forest through ecological changes, have suggested that Carpinus laxiflora is the leading tree type of the climax forest of temperate zone in South Korea. However, most of these studies on C. laxiflora forest have focused on vegetation structure and succession process in specific areas or regional physiognomical forest communities, and thus some may question whether it is rational to consider C. laxiflora forest as the climax forest in South Korea's temperate forest zone. The purpose of this study was to analyze the vegetation classification, species composition, and stratum structure and to investigate the succession tendency with the vegetation data collected from C. laxiflora forests in 75 quadrates in 17 mountains between 2014 and 2017. We used the phytosociological method to analyze the vegetation structure and the importance value to analyze the stratum structure. The results showed the floristic composition of 6 vegetation units and 9 species groups. The hierarchy of the C. laxiflora community group was represented by the Ulmus laciniata community, Corylus heterophylla community (subdivided Quercus aliena group and Corylus heterophylla typical group), and Rhododendron schlippenbachii community (subdivided Fraxinus chiisanensis group, Carpinus tschonoskii group, and Rhododendron schlippenbachii typical group). Successional tendency of C. laxiflora forest based on the importance of each layer is predicted to be the total of three types such as to maintain the stand (vegetation units 2, 3, and 6), to success the Carpinus cordata forest (vegetation units 1 and 4), and to success Quercus acuta forest (vegetation unit 5).

• Korean Journal of Food Science and Technology
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• v.29 no.4
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• pp.671-679
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• 1997

Approximate composition and mineral content of eighty seven medicinal herbs marketed in Korea was analyzed on the basis of their dried weight. From the results anlyzed, it was known that Acanthopanax sessiliflorum Seem. (22.4%), Portulaca oleracea L. (20.6%), Torilis japonica DC. (15.0%) in crude ash, Ziziyphus spinosa Hu. (39.9%), Alisma orientale Juzep. (30.6%), Lepidium latifolium L. (28.8%), Cassia tora L. (27.5%) in crude protein, Ziziyphus spinosa Hu. (27.9%), Xantnium strumarium L. (24.0%), Perilla frutescens var. acuta Kude (22.8%), Benincasa hispida Cong. (22.4%) in crude lipid, Vitex rotundifolia L. (59.5%), Ganoderma lucidum Karst (53.9%), Pinus densifiora Sieb. et Zucc. (46.4%), Sambucus williamsii Hanse (41.2%) in crude fiber, and Poria cocos Wolf (91.2%), Gastrodiae elata Blume (87.9%), Gentiana scabra Bunge (86.3%), Rehnannia glutinosa Libosch (86.0%) in nitrogen free extract exhibited higher content than other kinds. Phosphrous contents were 1.5, 1.1 and 1.0% in order of Taxillus chinensis Danser, Malva verticillata L., Nelumbo nucifera Gaeten, and magnesium contents were 0.7, 0.5 and 0.5% in the order of Saururus chinesis Baill., Salivia milltriorrhiza Bunge and Pueraria thunberglans Benth. Calcium contents of Cimicifuge heradeifolia Komarov, Acanthopanax sessiliflorum Seem. and Rhus vericiflura Stokes were 8.5, 7.1 and 3.0%, and potassium contents of Tricholoma matsutake Sing., Houttuynia cordata Thunb., Achyranthes japonica Nakai were 5.6, 5,4 and 4.7%, respectively. Sodium contents were 2.1, 0.6 and 0.3% in the order of Boshinakia rossica Fedtsch., Eucommia ulmoides Oliv. and Prunus mume Seib., and iron contents were 0.3, 0.2 and 0.2% in the order of Cirsium japonicum var, ussuriense Kitamuraa, Gentiana scabra Bunge and Phlomis umbrosa Turcz.

• Korean Journal of Environment and Ecology
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• v.32 no.1
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• pp.77-96
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• 2018

We investigated and analyzed three Korean island sites (Bijin-do, Ae-do, and Bogil-do) and one Japanese site (Tachibanayama) of sword-leaf litsea (Actinodaphne lancifolia) forests, known as the climax forest, to discuss the vegetation succession sere of warm-temperature evergreen broad-leaved forests. We then reviewed the literature in Korea, Japan, China, and Taiwan to consider the distribution characteristics of evergreen broad-leaved forests, vegetation succession sere, and climax tree species. Although Mt. Tachibana and Ae-do showed the most advanced vegetation structure, the soil and ordination (CCA) analysis indicated that it was not enough to consider that the sword-leaf litsea forest was at the climax stage in the warm-temperature region. The Actinodaphne lancifolia forest is sparsely distributed in Korea and Japan while the common types of vegetation in the warm temperate zone region in East Asia are Machilus spp., Castanopsis spp., and Cyclobalanopsis spp. The vegetation succession sere of the Korean warm-temperature region is thought to have a secondary succession such as Pinus thunbergii, P. densiflora, Q. serrata (early stage) through Machilus thunbergii, innamomum yabunikkei, Neolitsea sericea, Actinodaphne lancifolia (middle stage) to Castanopsis sieboldii, Q. acuta, Q. salicina (climax stage). However, Machilus thunbergii will be the climax species as an edaphic climax in places where there is a strong influence of the sea wind, or it is difficult to supply the seeds of Castanopsis spp. and Cyclobalanopsis spp.

• Journal of the East Asian Society of Dietary Life
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• v.22 no.2
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• pp.271-277
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• 2012

Ten green leafy vegetables were cultivated from medicinal plant seed. Their nutritional content, taste, appearance and texture were evaluated. Moisture content was $91.23{\pm}0.00%$ in $Saururus$ $chinensis$ (Sc), $90.39{\pm}0.05%$ in $Perilla$ $frutescens$ (Pa) and $66.78{\pm}0.05%$ in $Agastache$ $rugosa$ (Ar). The fat content was 8.89% in $Salvia$ $plebeia$ (Sp) and 0.13% in $Peucedanum$ $japonicum$ (Pj). The protein content was $11.1{\pm}0.00%$ in Ar and $0.54{\pm}0.03%$ in Pj. Total sugar content was $20.06{\pm}0.03%$ in $Geum$ $japonicum$ (Gj), $12.73{\pm}0.03%$ in $Coixlacry$ $majobi$ (Cm) and $1.04{\pm}0.03%$ in $Atractylodes$ $japonica$ (Aj). Medicinal leafy vegetables had higher glucose, sucrose and fructose, but lower maltose. Ascorbic acid content was $33.67{\pm}1.40$ mg% in Sc, $27.91{\pm}2.91$ mg% in Pa and $15.68{\pm}1.09$ mg% in At. Chlorophyll content was higher in $Perilla$ $frutescens$ var. $acuta$ Kudo than other leafy vegetables. The evaluation also showed that Cm, Pa and Aj tasted sweeter and had low acidity.

• Korean Journal of Environment and Ecology
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• v.32 no.3
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• pp.289-302
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• 2018

The study investigated the forest vegetation in 59 plots between June 2017 and August 2017 to understand the forest vegetation structure of the protected zone for forest genetic resource conservation (forest genetic resource reserve area) in Heuksando Island. We classified the vegetation using the Z-M phytosociological method analyzed the importance value and species diversity of each vegetation classification. The analysis showed the Camellia japonica community group at a top level of forest vegetation hierarchy. In the level of community, it was classified into Dendropanax morbiferus community (Vegetation unit 1; VU 1), Carpinus turczaninowii community, and C. japonica typical community (VU 6). C. turczaninowii community was subdivided into Buxus koreana group (VU 2), Rhododendron mucronulatum group (VU 3), Vitis amurensis group (VU 4) and C. turczaninowii typical group (VU 5). Therefore, it was classified into a total of six vegetation units (one community group, three communities, and four groups). The analysis of the mean codominant value of each VU show that Quercus acuta was the highest in VU 1, C. turczaninowii in VU 2, Pinus thunbergii in VU 3, Pinus densiflora in VU 4, and Castanopsis sieboldii in VU 5 and VU 6. The analysis of species diversity showed that VU 2 was the highest among six units in species richness index, species diversity index, and species evenness index. VU 6 showed the highest among six units in species dominance index. In conclusion, a synecology approach to manage six units and twelve species groups was needed for the forest vegetation of Heuksando Island protected area for forest genetic resource conservation.

• Korean Journal of Plant Resources
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• v.31 no.4
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• pp.330-341
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• 2018

This study was performed to investigate and measure the antimicrobial activity of evergreen woody species extracts on Trichophyton mentagrophytes. To do this, leaves and stems were collected from Wando and Jeju islands, and were used for the extraction with different solvents (i.e., distilled water, 80% ethanol, and 100% methanol), and at different ultrasonic extracting times (i.e., 15, 30, and 45 minutes). The experiment was conducted by using the agar diffusion method. The clear zone was measured after incubating the paper disc containing the plant extract in a bacterial culture medium. The controls were synthetic antimicrobials, methylparaben and phenoxyethanol, at concentrations of 0.4, 1, 2, and 4 mg/disc. Altogether, extracts of 56 out of 64 species used in this study had inhibitory activity, which confirmed their antimicrobial activity against Athlete's foot. Among them, the crude ethanolic extract of Elaeocarpus sylvestris in 45 min showed a zone of inhibition < 20.2 mm, while the clear zone of Actinodaphne lancifolia ethanolic extraction for 30 min was 23.5 mm. Also, Quercus acuta, Dendropanax morbiferus and Daphne odora showed clear zones of 28.0 mm (45 minutes ethanolic extraction), 20.5 mm (45 minutes crude methanolic extraction) and 19.7 mm (45 minutes methanolic extraction), respectively. Thus, these results confirm that the extracts of evergreen woody species have therapeutic potential against Athlete's foot, and suggest that in order to extract adequate amounts of antimicrobial substance from the plant sources, ideal extraction condition has to be considered.