• KIISE Transactions on Computing Practices
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• v.22 no.3
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• pp.139-144
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• 2016

There exist many threats in cyber space, however current anti-virus software and other existing solutions do not effectively respond to malware that has become more complex and sophisticated. It was shown experimentally that it is possible for the proposed approach to provide an automatic execution environment for the detection of malicious behavior of active malware, comparing the virtual-machine environment with the real-machine environment based on user interaction. Moreover, the results show that it is possible to provide a dynamic analysis environment in order to analyze the intelligent malware effectively, through the comparison of malicious behavior activity in an automatic binary execution environment based on real-machines and the malicious behavior activity in a virtual-machine environment.

• Korean Journal of Human Ecology
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• v.8 no.2
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• pp.327-337
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• 1999

Primitive men's primordial formative activity derives from incarnation contained in intensive vitality and strong creativity mind. The trend of modern fashion requiring the boundless imagination can be called that it, just, itself, has something in common with basic spiritual activity that primitive men had. What is concerning how modern fashion designers have developed formative language through enchanting primitive art is as follows. 1. The nature's mysterious power appears to be transformed into personified image. The incarnation for acquiring beauty, power, prestige of genius appears in the form of personification, making use of mask in modern fashion. 2. As for primitive men, the whole universe has a potentiality as a symbol. The symbolism of primitive art based on religion and myth appears in modern art as the form of presenting oppositional objects together in one space. 3. Primitive art is the purest form and the most unpolluted. This shows the natural quality being assimilated into nature, which is expressed in modern fashion as intensity, free-spirit, simplicity, etc. 4. The primitive men's anxiety to the outside world appears as impulse. The geometrical form of primitive art occurred in the shape of impulse appears in expressional form of modern fashion. 5. The real existence in primitive art inducing real materials and objects, in themselves, into formative world appears in modern art in the way of expression such as repetition, enlargement, exact reproduction.

• Hwankyungkyoyuk
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• v.24 no.4
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• pp.62-77
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• 2011

The purpose of this study is to overcome the realistic pressure and the limit of school environmental education and to develop an environmental education program that can provide students, who live in an urban area, have difficulty in contacting with nature, with an opportunity to experience the nature in the school outdoor environment, which is their daily living space and is a ground of environmental education. First, I developed an analytical tool which can be used to determine the degree how the environmental education is related to the school outdoor environment with 8 categories(spaces). And I analyzed the 2007-79 curriculum of lower grade(1 and 2 grades) of the elementary school provided by the Ministry of Education and Human Resources Development by using the analytical tool and found out that this curriculum was suitable for the environmental education utilizing the school outdoor environment. According to this curriculum analysis, the studying subjects which could be suitable for the environmental education utilizing the school outdoor environment were extracted, and based on these subjects, an environmental education program was developed. The environmental education 'in' environment program were composed with 5 modules and 18 activity subjects suitable for students advancement phase, and these activities can be implemented with experiencing, understanding and expressing by using 5 senses. I also proposed a plan that can be used to apply this program continuously to the lower grades(1 and 2 grades) original experience activity class.

• The Bulletin of The Korean Astronomical Society
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• v.42 no.2
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• pp.83.1-83.1
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• 2017

SDO/AIA와 STEREO/EUVI 두 태양 관측 위성의 193 파장에서의 실시간 영상 이미지를 이용하여 Stonyhurst Heliographic Map을 작성하고 각각의 위성 데이터 분석으로부터 얻어진 결과들을 종합적으로 재구성하여 태양 전면 및 후면의 활동 영역들을 동시에 표출하는 태양 활동성 지도 (Solar Activity Monitoring Map)를 작성하는 프로그램을 제작하였다. 태양 활동성 지도를 이용하여 태양 후면에서의 극자외선 밝기 분포를 경도에 따라 등간격으로 나눈 후 각 지역에서 얻은 극자외선량을 실시간으로 갱신하며 그래프를 작성하는 프로그램도 함께 제작하고 그로부터 태양 후면 영역의 활동성이 향후 지구에 어떠한 방식으로 영향을 미칠 것인지 사전에 예측 가능하도록 하였다. 또한 태양 후면에서 발생하는 활동 영역 (Active Region) 및 코로나홀들을 자동적으로 탐지한 후 실시간으로 변화 정도를 추적 및 기록하는 프로그램도 제작하였다. 태양 활동성 지도는 193 파장에서 뿐만 아니라 두 위성이 공유하는 세 개의 동일 혹은 유사한 파장대 (171,211,304)에서 얻어진 데이터들도 함께 이용하여 각 파장대에서 독립적으로 작성하였는데 이로 인해 각각의 에너지 영역의 특성에 해당하는 태양 활동성을 동시에 표출하는 것이 가능하게 되었다. 이러한 프로그램을 이용하여 태양 후면에서의 활동 영역의 발생 및 변화를 사전에 인식하고 그들이 태양 전면으로 나타나기 전에 대비할 수 있는 예보 장치가 마련되었다. 본 연구들과 더불어 극자외선 영역에서의 태양 활동성 조사로부터 플레어의 발생을 예측할 수 있을 것인지의 가능성 여부를 타진하기 위해 과거 극자외선 관측에서 얻어진 활동 영역들의 데이터와 연 X-선 관측으로부터 기록된 플레어 발생 여부의 상관관계를 조사하는 연구가 현재 진행 중이다. 이러한 연구로부터 긍정적인 결과가 도출되는 경우 극자외선 영역에서의 관측 데이터를 이용하여 플레어 발생 가능성을 예측하는 새로운 방법을 제시하는 것이 가능해질 것이다.

• BMB Reports
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• v.47 no.10
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• pp.546-551
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• 2014

The insecticidal activity of Bacillus thuringiensis (Bt) Cry toxins involves toxin stabilization, oligomerization, passage across the peritrophic membrane (PM), binding to midgut receptors and pore-formation. The residues Arg-158 and Tyr-170 have been shown to be crucial for the toxicity of Bt Cry4Ba. We characterized the biological function of these residues. In mosquito larvae, the mutants R158A/E/Q (R158) could hardly penetrate the PM due to a significantly reduced ability to alter PM permeability; the mutant Y170A, however, could pass through the PM, but degraded in the space between the PM and the midgut epithelium. Further characterization by oligomerization demonstrated that Arg-158 mutants failed to form correctly sized high-molecular weight oligomers. This is the first report that Arg-158 plays a role in the formation of Cry4Ba oligomers, which are essential for toxin passage across the PM. Tyr-170, meanwhile, is involved in toxin stabilization in the toxic mechanism of Cry4Ba in mosquito larvae.

• KSBB Journal
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• v.11 no.1
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• pp.37-45
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• 1996

Marine bacterial strain, highly effective agar degrading, was isolated from south sea of Korea and was identified as Pseudomonas sp. This strain was named Halophilic Pseudomonas sp. W7 and accumulated an extracellular agarase which showed a high level of enzyme activity in the presence of agar and agarose. This extracellular agarase was purified by anion-exchange chromatography and gel filtration. Purified agarase showed a single protein band upon sodium dodecyl sulfate-polyacrylamide gel electrophoresis and its molecular weight was estimated to be about 89KDa. The agarase gene was cloned into Escherichia coli JM83 using the plasmid vector pUC19. DNA fragments(3.7, 3.0Kb) of Hind III-digested chromosomal DNA of Pseudomonas sp. W7 was inserted into the Hind III site of pUC19. Selected transformants, E. coli JM83/pSWl 000000and E. coli JM83/pSW3, produced agarase and this agarase was accumulated In the cytoplasmic space.

• Korean Journal of Microbiology
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• v.38 no.4
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• pp.218-218
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• 2002

Using two drugs, tunicamycin and brefeldin A, which affect protein processing, we investigated the intracellular processing mechanism of secreted aspartyl proteinase 1 (SAPl) of Candide albicans. Three intracellular forms of SAPI were detected by immunoblotting using menoclonal antibody (MAb) CAPl. Their molecular weights were approximately 40, 41 and 45 kDa, respectively. The 41 kDa protein is a glycoprotein and may be the same as the extracellular form judging by its molecular mass. The 40 kDa protein was the unglycosylated form and its molecular mass coincided with deglycosylated SAPl and the 45 kDa protein was also the unglycosylated form. Neither the 40 and 45 kDa proteins were detected in the culture supernatant of C. albicans. These suggested that the 40 and 45 kDa proteins might be intracellular precursor forms of SAPI. These results show that SAPI is translated as a 45 kDa precusor form in the endoplasmic reticulum and the 45 kDa precursor farm undergoes proteolytic cleavage after translocation into the Golgi apparatus, generating the 40 kDa precursor form. This 40 kDa precursor is converted into a 41 kDa mature form through glycosylation in the Golgi apparatus. The mature form of the 41 kDa protein is sorted into secretary vesicles and finally released into the extracellular space through membrane fusion. When the glycan region of SAPl was digested with N-glycosidase F, both stability and activity of the enzyme decreased. These results indicate that the glycan attached to the enzyme may, at least in parti be related to enzyme stability and activity.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2013.05a
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• pp.175-176
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• 2013

Currently barbola has been considered in Korea. The artistry of it has been studied. Interior design barbola has developed from an art activity to a real life product. Interior design is meant to total design activity to satisfy all condition both function and emotion suited for the goal of interior space human dwells. We study the product technique of optic media barbola sculpture with affection and refinement to use traditional superior onion on optic media and permeability in order to apply the artistry of barbola to interior design. We make real sculpture using it. The mineral electric radiation sheet in this research includes 0.2mm thickness with low power, high brightness, and non-heat. The result of this study contains a new type and narrative beyond the limitation of the traditional barbola. We make power saving lighting sculpture through it.

• Proceeding of Spring/Autumn Annual Conference of KHA
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• 2008.04a
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• pp.421-426
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• 2008

In case of the large-scaled indoor gymnasium that has been constructed in the local area, there are many instances for the use as multipurpose space where the public performances are possible such as leisure activity, lecture, assembling activity, drama, concert and so on for the resident together with the purpose of sporting facility. In order for utilization to the maximum of the function of such indoor gymnasium, the acoustic capabilities concerned with Definition of both Voice and Music are simultaneously required. However, in case of the large-scaled athletic facility, since it was designed with high ceiling-height in view of its characteristics, it forms a Sound Focus and then the sound is concentrating to the specific part, and because the vibration of sound is too loud due to its broad volume, the acoustic defects such as many Echoes are arising. On such viewpoint, based on the drawing of the indoor gymnasium that is scheduled to be built at B County, Chonbuk Province, this Study has proposed such indoor gymnasium equipped with the optimized acoustic condition passing through Acoustic Simulation Phase. As the result of Acoustic Simulation, we could design the indoor gymnasium that equipped with really satisfying acoustic performance 'after' reformation compared with 'before' reformation, and it is considering that such material could be utilized as the fundamental material that brings a curtailment effect of the construction cost and also enables us to improve the acoustic performance, at the stage of planning and designing for the similar sporting facility in the future.

• Archives of Pharmacal Research
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• v.3 no.1
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• pp.7-12
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• 1980

Ampliciline was synthesized from 6-amino-pencillanic acid (6-APA) and D-.alpha. phenylglycine methyl ester by using amplicilin synthesizing enzyme from Peudomonas melanogenum (IAM 1655). The whole cell enzyme was immobilized by entrapping it in the polyacrylamide gel lattices. The polymer used in the enzyme entrapment was made from 150 mg per ml of acrylamide monomer and 8 mg per ml of N, N'-methylenebisacrylamide. About 200 mg/whole cell enzyme was mixed in the polymer for entrapment. The maximal activity retention after immobilization was 56%. The optimal pH values for the whole cell enzyme and the immobilized whole cell enzyme were 6.0 and 5.9, respectively. The optimal temperature for the enzyme activity were the same for both type of preparations. The enzyme stabilities against pH and heat increased for immobilized whole cell enzyme. Immobilized cell was more stable especially in the acidic condition while both type were found to be very suceptible to thermal inactivation at a temperature above 4.deg.C. The kinetic constants obtained from Lineweaver-Burk plot based on two substate reaction mechanism showed somewhat higher value for immobilized whole cell enzyme as compared to the whole cell enzyme : the Km value for 6-APA were 7.0 mM and 12.5 mM while Km values for phenylglycine methyl ester were 4.5 mM and 8.2 mM, respectively. Using the immobilized whole cell enzyme packed in a column reactor, the productivity of ampiciline was studied by varying the flow rate of substrate solution. At the space velocity, SV, 0.14 hr$^{-1}$ the conversion was 45%. Operational stability found in terms of half life was 30 hr at SV = 0.2 hr.