• Journal of Life Science
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• v.27 no.12
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• pp.1403-1409
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• 2017

In this study, the xylitol dehydrogenase (XYL2) gene was expressed in Saccharomyces cerevisiae as a host cell for ease of use in the degradation of lignocellulosic biomass (xylose). To select suitable expression systems for the S.XYL2 gene from S. cerevisiae and the P.XYL2 gene from Pichia stipitis, $pGMF{\alpha}-S.XYL2$, $pGMF{\alpha}-P.XYL2$, $pAMF{\alpha}-S.XYL2$ and $pAMF{\alpha}-P.XYL2$ plasmids with the GAL10 promoter and ADH1 promoter, respectively, were constructed. The mating factor ${\alpha}$ ($MF{\alpha}$) signal sequence was also connected to each promoter to allow secretion. Each plasmid was transformed into S. cerevisiae $SEY2102{\Delta}trp1$ strain and the xylitol dehydrogenase activity was investigated. The GAL10 promoter proved more suitable than the ADH1 promoter for expression of the XYL2 gene, and the xylitol dehydrogenase activity from P. stipitis was twice that from S. cerevisiae. The xylitol dehydrogenase showed $NAD^+$-dependent activity and about 77% of the recombinant xylitol dehydrogenase was secreted into the periplasmic space of the $SEY2102{\Delta}trp1/pGMF{\alpha}-P.XYL2$ strain. The xylitol dehydrogenase activity was increased by up to 41% when a glucose/xylose mixture was supplied as a carbon source, rather than glucose alone. The expression system and culture conditions optimized in this study resulted in large amounts of xylitol dehydrogenase using S. cerevisiae as the host strain, indicating the potential of this expression system for use in bioethanol production and industrial applications.

• Korean Journal of Remote Sensing
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• v.34 no.6_4
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• pp.1545-1553
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• 2018

Recent eruptive activity at Kīlauea Volcano started on at the end of April in 2018 showed rapid ground deflation between May and June in 2018. On summit area Halema'uma'u lava lake continued to drop at high speed and Kīlauea's summit continued to deflate. GPS receivers and electronic tiltmeters detected the surface deformation greater than 2 meters. We explored the time-series surface deformation at Kīlauea Volcano, focusing on the early stage of eruptive activity, using multi-temporal COSMO-SkyMed SAR imagery. The observed maximum deformation in line-of-sight (LOS) direction was about -1.5 meter, and it indicates approximately -1.9 meter in subsiding direction by applying incidence angle. The results showed that summit began to deflate just after the event started and most of deformation occurred between early May and the end of June. Moreover, we confirmed that summit's deflation rarely happened since July 2018, which means volcanic activity entered a stable stage. The best-fit magma source model based on time-series surface deformation demonstrated that magma chambers were lying at depths between 2-3 km, and it showed a deepening trend in time. Along with the change of source depth, the center of each magma model moved toward the southwest according to the time. These results have a potential risk of including bias coming from single track observation. Therefore, to complement the initial results, we need to generate precise magma source model based on three-dimensional measurements in further research.

• Publications of The Korean Astronomical Society
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• v.32 no.1
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• pp.201-203
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• 2017

There exists strong evidence supporting the co-evolution of central supermassive black holes and their host galaxies; however it is still under debate how such a relation comes about and whether it is relevant for all or only a subset of galaxies. An important mechanism connecting AGN to their host galaxies is AGN feedback, potentially heating up or even expelling gas from galaxies. AGN feedback may hence be responsible for the eventual quenching of star formation and halting of galaxy growth. A rich multi-wavelength dataset ranging from the X-ray regime (Chandra), to far-IR (Herschel), and radio (WSRT) is available for the North Ecliptic Pole field, most notably surveyed by the AKARI infrared space telescope, covering a total area on the sky of 5.4 sq. degrees. We investigate the star formation properties and possible signatures of radio feedback mechanisms in the host galaxies of 237 radio sources below redshift z = 2 and at a radio 1.4 GHz flux density limit of 0.1 mJy. Using broadband SED modelling, the nuclear and host galaxy components of these sources are studied simultaneously as a function of their radio luminosity. Here we present results concerning the AGN content of the radio sources in this field, while also offering evidence showcasing a link between AGN activity and host galaxy star formation. In particular, we show results supporting a maintenance type of feedback from powerful radio-jets.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.18 no.1
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• pp.85-94
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• 2017

This study researched the meaning of the outdoor play spaces used by young children, as perceived by them and their teachers. For this purpose, indirect observation, nonformal interviews during the activity of young children and in-depth interviews with teachers were conducted in 3 kindergartens and 1 day care center which operate various programs. As a result, outdoor play spaces were recognized as a psychological space, expanded space and sequential space for the relationships of young children. Meanwhile, early childhood teachers perceived them as a functional space, expanded space and restricted space of the children's daily lives. It was recognized that outdoor play spaces provided the children with good and unstructured environments to reorganize their own daily pattern through expanded play and the chance to naturally form peer relationships. This suggests that the space configuration which is most suitable for child centered development is one that is formed within and by their daily lives, rather than the standardized configuration of outdoor play spaces.

• Review of Culture and Economy
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• v.17 no.2
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• pp.161-184
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• 2014

The dramatic rise of experience space using cultural heritage in modern architecture has been generally acknowledged in recent literature. The present study aims to investigate effective ways to enhance competitiveness in local experience space, with an emphasis on the use of cultural heritage in modern architecture. Using the case of the Sam-Rae Arts Village in Wanju-gun, Jeollabuk, this study analyzes the characteristics of the 4Es in experience economy theory. In order to arrive at findings that represent the variety of perspectives found in this context, qualitative interviews were conducted with three groups: public officials, maintenance staff in the experience center, and field experts. The results of this study revealed that the Visual Media Art Museum and Book Wooks were used for entertainment, the Corpentry Shop for education, the Book Museum for escapism, and the Design Museum Culture $Caf{\acute{e}}$ The O's for aestheticism. Additionally, depending on the commercial potential of the respective experience space, the Corpentry Shop, Book Wooks, and the Culture $Caf{\acute{e}}$ The O's were used for profit purposes while the Design Museum and the Visual Media Art Museum for non-profit. Based on the findings of the present study, effective ways to enhance competitiveness in the local experience space are suggested. First, the public nature of cultural enjoyment may be realized in a field that bears non-profit characteristics. In exhibiting works, the experience space could suggest the extra demonstration. Second, in the for-profit experience hall, the securing of tourists may help to maximize profit. In so doing, a variety of experience programs and activity spaces could be provided internally, while advertisement marketing through mobile and SNS could be reinforced externally.

• Korean Journal of Remote Sensing
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• v.35 no.2
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• pp.241-250
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• 2019

The current U.S. remote sensing act was made in 1992 and has been criticized for being outdated and inappropriate in view of the modern technological development. In order to enhance the American competitiveness and leadership in the world, President Trump announced Space Policy Directive (SPD) - 2 on May 24, which is designed to modernize the regulations related to commercial space activities including private remote sensing system operations. It should be noted that the regulatory reform efforts are made within broader terms of the National Security Strategy on Dec. 17, 2017, pursuing the enhancement of national security and economic prosperity as well. A legislative support in Congress has also been added to the Administration's efforts. The proposed regulatory reform on the licensing of commercial remote sensing system operations outlines the features of lessening administrative burden on applicants by simplifying the overall application process and of limiting the operations only when there is an impact upon the national security with clear and convincing evidence. But, due to a different regulatory system of each country, such a movement to expand an individual's freedom to explore and utilize outer space may result in an international dispute or a violation of international obligations, so there should be a merit in paying attention to the U.S. commercial remote sensing regulatory reform, and it is desirable to establish international norms as flexible and appropriate to the level of space technology and space industry.

• Journal of the Korean Institute of Landscape Architecture
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• v.52 no.4
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• pp.56-68
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• 2024

This study aimed to derive the concept and types of school outdoor spaces in Seoul. The research results showed that the concept of school outdoor space can be viewed as an outdoor activity space in schools that secures the value of integrated public life and communal life, including "behavioral rights, walking rights, living rights, urban rights, landscape rights (viewing rights), and nature experience rights." It can also be defined as a basic educational space that reflects the value of education, which is the basic function of schools and the characteristics of the community. As a result of the analysis based on the general types of outdoor spaces, it was found that the outdoor spaces of Seoul schools can be divided into three major categories: walking space (movement function), educational space (educational activities), and rest and other spaces (other services). Based on this, outdoor spaces were categorized and subcategorized to serve as the basis for policy implementation. These classifications are characterized by 1) simplifying the classification of spaces by function into three (movement functions, educational activities, and other services) to enable the use of outdoor spaces for integrated purposes; 2) reflecting the complex use of spaces by subdividing educational spaces focusing on major activities, and in particular, discovering complex spaces due to the three-dimensionalization of buildings; and 3) enabling spatial-level planning and facility element-level access according to school characteristics. It is characterized by applying the recent trends and systems of general outdoor space projects to the current situation of schools.

• Applied Biological Chemistry
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• v.38 no.4
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• pp.359-363
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• 1995

New ten N-[1-(benzotriazol-1-yl)alkyl]aniline(4) derivatives were synthesized and the crystal structure of 4h was shown by X-ray crystallography and the absolute configuration has been assigned as S form. The molecule crystallizes in the monoclinic system, space group $P2_{1}/n$. And the molecules in the crystal are linked with each other through the hydrogen bond $(N_{11}-H_{11}{\cdots}N3)$ with distance $2.300(11){\AA}$ The fungicidal activity($pI_{50}$) in-vitro against Botrytis cineria (BC), Phytophthora casici (PC) and Sclerotium cepinorum (SC) were determined by the agar dilution method. The structure activity ralationships (SAR) between structure of 4 and the activity were studied using a physicochemical parameters of substituents and multiple regression technique. Among these compounds, only the bromo group substituent(4f) showed higher activity, which depend on the hydrophobic(${\pi}$) of substituents. The relative orders of the activity are SC>BC> and PC, respectively. This implies that the activity is affected by the hydrophobic(${\pi}$) nature of the Z group rather than the X group. Linear free energy relationships(LFER) on the fungicidal activity with substituents has been also discussed.

• Korean Journal of Plant Resources
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• v.27 no.5
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• pp.429-438
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• 2014

This study was designed to investigate the effects on anti-oxidative activities and increasing extraction yield of Aralia elata Cortex by gamma irradiation. Electron spin resonance (ESR) analysis as physical techniques for irradiation identification of Aralia elata showed that a pair of peak appeared on a space of 6.0 mT at the left and right of symmetric unspecific central ESR spectrums, confirming that the plant was gamma-irradiated. The optimum extracting conditions for preparing gamma irradiated samples from Aralia elata Cortex were to extract with 50% ethanol for 15 hrs after 10 kGy irradiation. DPPH scavenging activity and ABTS radical cation inhibitory activity of the water and 50% ethanol extracts from non irradiated and irradiated Aralia elata Cortex was very high as over 80% and 98%, respectively, at tested low concentration of $50{\mu}g/mL$. Antioxidant protection factor (PF) as anti-oxidation indicator of lipophilic compounds showed a very high level of activity as 2.18~2.78 PF. As for TBARs, water and ethanol extracts showed high level. Increase of TBARs inhibitory activity of water extracts was not shown by gamma-ray irradiation but ethanol extracts showed slight increasement of TBARs inhibitory activity with 10 kGy gamma-ray irradiation. These results shown confirmed increasement of extraction yield for phenolic compounds and anti-oxidative activity from Aralia elata. Thus, the treatment of gamma-irradiation can be used a way to amplify a solubility for biological active compounds and anti-oxidative activity in plants.

• Proceedings of the KSRS Conference
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• 2008.10a
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• pp.348-357
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• 2008

The classification of the effect of ionospheric disturbances on the radio occultation signal amplitude has been introduced based on an analysis of more than 2000 seances of radio occultation measurements per formed with the help of the CHAMP German satellite. The dependence of the histograms of variations in the radio occultation signal amplitude on the IMF variation index has been revealed. It has been indicated that it is possible to introduce the radio occultation index characterizing the relation between ionospheric disturbances and solar activity. An amplitude radio occultation (RO) method is proposed to study connection between the ionospheric and solar activity on a global scale. Sporadic amplitude scintillation observed in RO experiments contain important information concerning the seasonal, geographical, and temporal distributions of the ionospheric disturbances and depend on solar activity. The probability of strong RO amplitude variations (RO $S_4$ index greater than 0.2) in the CHAMP RO signals diminishes sharply with the weakening of solar activity from 2001 to 2008. The general number of RO events with strong amplitude variations can be used as an indicator of the ionospheric activity. We found that during 2001-2008 the daily globally averaged RO $S_{4a}$ index depends essentially on solar activity. The maximum occurred in January 2002, minimum has been observed in summer 2008. Different temporal behavoir of $S_{4a}$ index has been detected for polar (with latitude greater than $60^{\circ}$) and low latitude (moderate and equatorial) regions. For polar regions $S_{4a}$ index is slowly decreasing with solar activity. In the low latitude areas $S_{4a}$ index is sharply oscillating, depending on the solar ultraviolet emission variations. The different geographical behavoir of $S_{4a}$ index indicates different origin of ionospheric plasma disturbances in polar and low latitude areas. Origin of the plasma disturbances in the polar areas may be connected with influence of solar wind, the ultraviolet emission of the Sun may be the main cause of the ionospheric irregularities in the low latitude zone. Therefore, the $S_{4a}$ index of RO signal is important radio physical indicator of solar activity.