• Journal of Chest Surgery
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• v.15 no.1
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• pp.21-26
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• 1982

Fourteen cases of funnel deformity, 11 were male and the others female, treated over a eleven-year period, are presented. The overall results with the methods described by the authors, Wada, Shannon, Adkins and Ravitch appear to be excellent. The symmetric depression was more common In children under the age of 12 years and asymmetric one Increased after the age of 12. Six cases of abnormal cardiac auscultatory findings revealed no consistent hemodynamic abnormalities. An 21-year-old female was associated with left lung agenesis and dextroversion of the heart, and a 6-year-old boy with congenital bronchogenic cyst who underwent right upper lobectomy. EKG changes associated with the anomaly were observed in all. Pulmonary function test showed the range from normal to moderate restriction which did not Improve in only one patient after operation, but the patient did not complain any restriction In activity. Some transient complications were developed which resulted in improvement.

• Korean Journal of Microbiology
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• v.24 no.4
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• pp.406-410
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• 1986

The isolation and charateriation of a type II restriction endonuclease from Xanthomonas citri IFO 3835 were described. This enzyme (Xci I endonuclease) is an isoschizomer of Sal I endonuclease recognizing 5'-GTCGAC-3' and cleaving at the site indicated by the arrow. Unlike Sal I endonuclease, Xci I endonuclease requries a NaCl concentration of 50mM for its maximum activity.

• Microbiology and Biotechnology Letters
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• v.22 no.6
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• pp.599-606
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• 1994

Bacillus stearothermomophilus, a strong xylan degrader, was confirmed to express multiple esterase activities in addition to the major xylanolytic enzymes. One of the genes encoding the esterases was isolated from the genomic library of B. stearothermophilus constructed with EcoRl restriction endonuclease and pBR322 plasmid. Three recombinant plasmids showing the tributyrin degrading activity were selected from approximately 7, 000 E. coli HB101 transformants, and were found to have the same insert of a 3.2 kb DNA fragment. Restriction mapping and hybridization studies revealed that the gene(estII) on the hybrid plasmid (pKMG7) had originated from the B. stearothermophilus chromosome, and was distinct from the estl, another esterase gene of B. stearothermophilus isolated in the previous work. The E. coli cells harboring pKMG7 produced an acetylxylan esterase that exibited similar substrate specificity to the esterase encoded by the estI gene.

• Korean Journal of Microbiology
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• v.26 no.1
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• pp.32-36
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• 1988

The yeast gene HOM3 encodes aspartokinase, which catalyses the first step (aspartate to and from beta-aspartyl phosphate) of common pathway to threonine and methionine. The yeast HOM3 gene expression is known to be regulated by threonine and methionine specific control, and also by general control of amino acid biosynthesis. Isolation and characterization of the HOM3 gene are essential for the molecular genetic study on its regulation of expression. A recombinant plasmid pSC3 (15.5kb, vector YCp50) has been cloned into E. coli HB101 from yeast genomic library through their complementing activity of HOM3 mutation in a yeast recipient strain M34-24B. Organization of the plasmid was characterized by delineation of restriction cleavage sites in the insert fragment.

• Journal of Korean Society for Quality Management
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• v.32 no.4
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• pp.196-207
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• 2004

TDR(Tear Down & Redesign)means that renounce the past resolutely, from a white paper condition, let's change directions. That is, let's redesign from the basic of the establishment of the construction process. Through the switchover the conception, innovate totally the whole existing processes. TDR is the best core innovation activity. The ultimate aim of the TDR activity induces the employees to do more productive business and obtain excellent results in the future by solving the problems, which are the nonproductive business in the business process on the objective view, the business that customers don't recognize the value, the same old practice of business, and the unsettled business from the restriction of resources. By organizing the related to workers of those business, they can solve the problems for 3-4months by full time working This paper inquires what are the obstruction factors on the process of executing TDR activity. Through the search of the reformation plan for the improvement of immersion degree, it presents some methods to be successful business.

• Microbiology and Biotechnology Letters
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• v.23 no.2
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• pp.145-149
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• 1995

For the purpose of developing a new biodegradable detergent, we have isolated a gene encoding wide-range temperature applicable alkaline protease from Xanthomonas sp. YL-37 (Lee et al., 1994, Kor. J. Appl. Microbiol. Biotechnol.). An alkaline protease gene was isolated from the gene bank that was prepared from the chromosomal DNA of Xanthomonas sp. YL-37. From the results of agarose gel electrophoresis and a restriction enzyme mapping, a 2.7 kb DNA fragment containing the alkaline protease gene was inserted in the plasmid pUC9. Extracellular activity of a clone having alkaline protease gene was detected on SDS-polyacrylamide gel with activity staining assay. The molecular weight of alkaline protease was determined to be about 64 kDa from 11% SDS-PAGE analysis. Alkaline protease activity, produced from E. coli which harboring the plasmid, showed no difference at reaction temperature 20, 30 and 40$\circ$C, respectively. This result showed that alkaline protease produced from E. coli harboring the plasmid was apparently the same as that of Xanthomonas sp. YL-37.

• Proceedings of the IEEK Conference
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• 1999.06a
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• pp.381-384
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• 1999

In this paper, we addressed the problem of reducing the switching activity in pipeline datapath and proposed a solution. clock-gating method is a kind of practical technique for reducing switching activity in finite state machine. But, in the case that the target gated function unit has a pipeline structure, there is some spurious switching activity on each stage register group. This occur in early stage of every function enable cycle. In this paper we proposed a method to solve this problem. This method generates the enable signal to each pipeline stage to gate the clock feeding register group. Experimental results showed effective reduction of dynamic powers in pipeline circuits.

• Journal of Nutrition and Health
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• v.16 no.4
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• pp.243-252
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• 1983

This study was undertaken to invesigate the effect of early nutritional deprivation and environment on neurotransmitter concentrations and behavior in later life. The restoring process of rats fed foods ad libitum after 50% restriction of the casein or the Korean diet during the prenatal and/or the lactating periods was observed. There were two rearing conditions, isolated and enriched, after weaning. Behavioral development was measured by the Y- shaped water maze and the open field test. The neurotransmitters were analyzed after sacrifice at the age of 21 weeks. The results are summarized as follows. 1) The body weight impairment by dietary restriction during the prenatal and lactating periods could be restored within 18 weeks after weaning in case of living in a classical cage. The effect of quantitative restriction was bigger in the Korean diet than in the casein diet. 2) The brain weight was decreased by nutritional deprivation. Environmental enrichment increased it slightly. 3) The concentration of neurotransmitters, norepinephrine, dopamine, and serotonin, were not shown any traces of the dietary restriction at the age of 21 weeks. 4) In the maze test, the deprived rats made more errors than the nourished and the rats fed the Korean diet more than those fed the cascin dict. The environmental enrichment could decrease the number of errors. 5) In the open field test, the dietary deprived groups showed less reaction time, more squares entered in the field, and less number of fecal boli than the nourished among the environmentally isolated rats. However, rats living in the enriched cage without experience of nutritional stress showed the lowest emotionality and the elevated exploratory activity.

• Korean Journal of Microbiology
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• v.32 no.4
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• pp.301-305
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• 1994

In catalytic properties of the restriction enonuclease, SdiI, which was purified from Streptomyces diastatochromogenes, this enzyme was active at wide range between pH 7.0 and 12.5, and up to $60^{\circ}C$ and 500 mM of NaCl concentration. It was stable between 20^{\circ}C$ and $60^{\circ}C$, and essentially requires $MgCl_2$ for endonuclease activity. The restriction map of lambda DNA which was obtained by double digestion with various enzymes suggested SdiI to be an isoschizomer of XhoI. From the determination of restriction site based on DNA sequencing method, recognition and cleavage specificity of SdiI was concluded as: 5‘-C${\downarrow}$TCGA G-3' 3'-G AGCT${\uparrow}$C-5'

• Journal of the Korea Convergence Society
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• v.12 no.11
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• pp.359-369
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• 2021

In this study, a complex sample logistic regression analysis was performed to identify the factors affecting the activity restriction of 2,701 normal elderly and chronically ill elderly aged 65 and over using raw data from the 8th period of the National Health and Nutrition Examination Survey. It was found that the elderly with chronic disease felt more restricted in their activities than the normal elderly. Activity limiting factors in stroke and hypertension patients are subjective health status, economic level, stress perception, and moderate-intensity work and leisure. The factors limiting activity in patients with heart disease were subjective health status and economic level, and factors limiting activity in patients with joint disease were subjective health status and high-intensity work and leisure. Activity limiting factors for lung disease patients are education level, high intensity work and leisure, and endocrine system activity limiting factors include subjective health status, stress perception, high intensity work and leisure, and activity limiting factors for cancer patients. is subjective health status, stress perception, moderate-intensity work and high-intensity leisure. Rehabilitation programs and policy support are needed for the continuous participation of the elderly with chronic diseases.