• Journal of The Korean Association For Science Education
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• v.26 no.5
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• pp.660-673
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• 2006

There have been many attempts to determine the value and the role of school laboratory experiment, but it seems hard to find consensus among these attempts from the perspective of education. This difficulty seems mainly due to disagreement on the concept of education, which has caused an instrumental attitude considering the school laboratory only as a means of developing science or pursuing various functions of school. However, the Endogenous Theory of Education (ETE), which claims education as 'a form of life', has recently paved the way for laboratory experiment to be justified as an opportunity of 'educooperation' allowing students to experience the intrinsic values of education in the medium of science. According to this theory, it is not the detailed practicals but the whole context where the laboratory activity is situated that matters in revealing the inherent educational phenomena. Through this new perspective, I observed two biology laboratory classes in a high school and analyzed the pattern of teacher-student and student-student interactions. Some meaningful educooperation was found in students' chattering, which has been traditionally considered as merely noise in the classroom, rather than in teacher-student interactions. This study discusses the reasons for these findings in detail and culminates in suggesting ways for accentuating the educational aspect of school laboratory activity.

• Atmosphere
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• v.20 no.4
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• pp.495-503
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• 2010

Satellite data have an intrinsic problem due to a number of various physical parameters, which can have a similar effect on measured radiance. Most evaluations of satellite performance have relied on comparisons with limited spatial and temporal resolution of ground-based measurements such as soundings and in-situ measurements. In order to overcome this problem, a new way of satellite data evaluation is suggested with statistical tools such as empirical orthogonal function(EOF), and singular value decomposition(SVD). The EOF analyses with OMI and OMI HCHO over northeast Asia show that the spatial pattern show high correlation with population density. This suggests that human activity is a major source of as well as HCHO over this region. However, this analysis is contradictory to the previous finding with GOME HCHO that biogenic activity is the main driving mechanism(Fu et al., 2007). To verify the source of HCHO over this region, we performed the EOF analyses with vegetation and HCHO distribution. The results showed no coherence in the spatial and temporal pattern between two factors. Rather, the additional SVD analysis between $NO_2$ and HCHO shows consistency in spatial and temporal coherence. This outcome suggests that the anthropogenic emission is the main source of HCHO over the region. We speculate that the previous study appears to be due to low temporal and spatial resolution of GOME measurements or uncertainty in model input data.

• The Mathematical Education
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• v.60 no.3
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• pp.341-362
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• 2021

The purpose of this study is to explore the qualitative characteristics of pre-service teachers' motivation while they are participating in group activities for developing mathematical essay assessment problem and revising it. For this purpose, we analyzed individual factors about group learning activities as well as contextual factors about practical competency (in developing and revising mathematical essay assessment problem through collecting data of student responses to the problem). As results of data analyses, autonomy, among individual factors regarding group learning activities, was one of the main characteristics in attainment value, utility value, and intrinsic value, whereas task, authority, and grouping, among contextual factors regarding practical competency, appeared to have a positive impact on task value. These results suggest how to think of specific ideas and articulate them in designing a curriculum to develop student-evaluation expertise for pre-service teachers.

• Geophysics and Geophysical Exploration
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• v.12 no.1
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• pp.17-26
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• 2009

On Aogashima Island, a volcanic island located in the southernmost part of the Izu Seven Islands Chain, vector magnetic anomalies were obtained in a helicopter-borne magnetic survey. The purpose of this study was to understand the volcanic structure of Aogashima Island in order to mitigate future disasters. Commonly, to obtain the magnetic structure of a volcanic island, total intensity anomalies (TIA) have been used, even though they have intrinsic errors that have not been evaluated correctly. Because the total intensity magnetic anomaly (TIA) is not a physical value, it does not satisfy Maxwell's Equations, Laplace's Equation, etc., and so TIA is not suitable for any physical analyses. In addition, it has been conventionally assumed that TIA is the same as the projected total intensity anomaly vector (PTA) for analyses of TIA. However, the effect of the intrinsic error ($\varepsilon_T$ = TIA.PTA) on the analysis results has not been taken into account. To avoid such an effect, vector magnetic anomalies were measured so that a reliable analysis of Aogashima Island magnetization could be carried out. In this study, we evaluated the error in TIA and used vector anomalies to avoid this erroneous effect, in the process obtaining reliable analysis results for 3D, vector magnetization distributions. An area of less than 1 A/m magnetization was found in the south-west part of Aogashima Island at the depth of 1.2 km. Taking the location of fumarolic activity into consideration, the lower-magnetization area was expected to be the source of that fumarolic activity of Aogashima Island.

• Journal of Pharmacopuncture
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• v.22 no.2
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• pp.83-89
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• 2019

Introduction: Oxidative stress (OS) during uncontrolled hyperglycemia has a pivotal role in pancreatic dysfunction. Our study aimed to demonstrate that crocin can potentiate anti-oxidant defense systems of pancreatic cells to improve oxidative stress. Methods: Male Wistar rats were divided randomly into four groups: a normal group, a normal-treated group, a diabetic group and a diabetic-treated group (n = 6 rats per group). Diabetes was induced by a single dose of streptozotocin (45 mg/kg/IV). The treated groups received crocin daily for 8 weeks (40 mg/kg/IP). At the end of the experiment, rats were sacrificed and pancreas tissue was obtained. Subsequently, the concentrations of malondialdehyde (MDA), nitrate and glutathione as well as the enzymatic activities of catalase and superoxide dismutase (SOD) were determined in all animals. Data were analyzed by two-way ANOVA with appropriate post hoc testing and a probability value of P < 0.05 was considered to represent a statistically significant difference in mean values. Results: Uncontrolled hyperglycemia weakened the anti-oxidant system by decreasing SOD and catalase enzyme activity in pancreatic tissues and induced OS by increasing the MDA content in diabetic non-treated animals. Crocin potentiated the anti-oxidant defense system by increasing the activity of both SOD and catalase, and improved OS by diminishing MDA production in pancreatic cells of rats contained in the diabetic-treated group. Conclusion: Based on our results, it is concluded that uncontrolled hyperglycemia can weaken the anti-oxidant defense system and cause the development of OS. Also, crocin can improve OS in pancreatic cells by potentiating the anti-oxidant defense system.

• Journal of Ginseng Research
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• v.44 no.6
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• pp.770-774
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• 2020

Background: Fermentation has been shown to improve the biological properties of plants and herbs. Specifically, fermentation causes decomposition and/or biotransformation of active metabolites into high-value products. Polyacetylenes are a class of polyketides with a pleiotropic profile of bioactivity. Methods: Column chromatography was used to isolate compounds, and extensive NMR experiments were used to determine their structures. The transformation of polyacetylene in red ginseng (RG) and the production of cazaldehyde B induced by the extract of RG were identified by TLC and HPLC analyses. Results: A new metabolite was isolated from RG fermented by Chaetomium globosum, and this new metabolite can be obtained by the biotransformation of polyacetylene in RG. Panaxytriol was found to exhibit the highest antifungal activity against C. globosum compared with other major ingredients in RG. The fungus C. globosum cultured in RG extract can metabolize panaxytriol to Metabolite A to survive, with no antifungal activity against itself. Metabolites A and B showed obvious inhibition against NO production, with ratios of 42.75 ± 1.60 and 63.95 ± 1.45% at 50 µM, respectively. A higher inhibitory rate on NO production was observed for Metabolite B than for a positive drug. Conclusion: Metabolite A is a rare example of natural polyacetylene biotransformation by microbial fermentation. This biotransformation only occurred in fermented RG. The extract of RG also stimulated the production of a new natural product, cazaldehyde B, from C. globosum. The lactone in Metabolite A can decrease the cytotoxicity, which was deemed to be the intrinsic activity of polyacetylene in ginseng.

• Journal of Life Science
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• v.28 no.1
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• pp.50-57
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• 2018

Millettia erythrocalyx, a species of plant in the Fabaceae family, is widely distributed in the tropical and subtropical regions of the world, such as the Indies, China, and Thailand. The antiviral activity of flavonoids from M. erythrocalyx has been reported; however, the antioxidative and anticancer activities of M. erythrocalyx remain unclear. In this study, we evaluated the antioxidative and anticancer effects of ethanol extract of M. erythrocalyx (EEME) and the molecular mechanism of its anticancer activity in human hepatocellular carcinoma HepG2 cells. EEME exhibited significant antioxidative effects, with a concentration at 50% inhibition ($IC_{50}$) value of $2.74{\mu}g/ml$, as measured by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay; moreover, it inhibited cell proliferation in a dose-dependent manner in HepG2 cells. Cell cycle analyses showed that EEME induced HepG2 cell accumulation in the subG1 phase in a dose-dependent manner. EEME also induced apoptosis of HepG2 cells, with increases in apoptotic cells and apoptotic bodies, as detected by Annexin V and 4,6-diamidino-2-phenylindole (DAPI) staining, respectively. Treatment with EEME resulted in increased expression of First apoptosis signal (Fas), a death receptor, and Bcl-2-associated X protein (Bax), a proapoptotic protein, and the activation of caspase-3, 8, and 9, resulting in the cleavage of poly (Adenosine diphosphate-ribose) polymerase (PARP). Collectively, these results suggest that EEME may exert an anticancer effect in HepG2 cells by inducing apoptosis via both the intrinsic and extrinsic pathways.

• The Korean Journal of Pharmacology
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• v.28 no.1
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• pp.75-79
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• 1992

Higenamine, which is one of active component of Aconiti tuber, has been known to have positive inotropic effect through adrenergic beta-receptor. The effect of higenamine on norepinepharine or potassium induced contraction of pulmonary aorta in rabbit were studies. 1. The contraction of aortic strips induced by norepinephrine was suppressec by pre-or post-treatment of higenamine dose dependently and those effects of higenamine were prevented by propranolol. The $pA_2$ value of higenamine against propranolol calculated as 8.25. 2. The effect of higenamine was not affected by phentolamine. 3. Isoprotrenol has shown 10 times stronger vasodiatory effect on norepinephrine induced cntracture than that of higenamine but high concentration $(3.3{\times}10^{-6}\;M)$ of isoproterenol produce intrinsic activity. 4. Vasodilatory effect of higenamine or isoproterenol was not observed in potassium induced contacture of pulmonary aortic strips. These results strongly suggest that higenamine dilated the pulmonary vascular smooth muscle through stimulation of adrenergic beta-receptor.

• Journal of Microbiology and Biotechnology
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• v.15 no.5
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• pp.965-970
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• 2005

In our previous study [14], we found that heat-shock exposure did not stimulate the neutral trehalase activity in Sacchromyces cerevisiae KNU5377, but did in ATCC24858. Consequently, the trehalose content in KNU5377 became 2.6 times higher than that in ATCC24858. Because trehalose has been shown to stabilize the structure and function of some macromolecules, the present work was focused to elucidate the relationship between trehalose content of these strains and thermal stabilities of whole cells, through differential scanning calorimetry (DSC), and to predict critical targets calculated from the hyperthermic cell killing rates. These analyses showed that the prominent DSC transition of both strains gave identical $T_m$ (transition temperature) values in exponentially growing cells, and that the $T_m$ values of critical targets was about $3^{\circ}C$ higher in KNU5377 than in ATCC24858. Both heat-shocked KNU5377 and ATCC24858 cells displayed similar shifts in their DSC transition profiles. On the other hand, the $T_m$ value of the critical target of KNU5377 was decreased by $2.1^{\circ}C$, which was still higher than ATCC24858 showing no changes. In view of these results, the intrinsic thermotolerance of KNU5377 did not appear to result from the stability of entire cellular components, but rather possibly from that of particular macromolecules, including critical targets, even though it should be investigated in more details. Although the trehalose levels in heat-shocked cells are significantly different, as described in our previous study [14], the overall pattern of thermal stabilities and their predicted critical targets in two heat-shocked strains seemed to be identical. These data suggest that the trehalose levels examined before and after heat shock of exponentially growing cells are not closely correlated with the stabilities of whole cells and/or critical targets in both yeast strains.

• The Korean Journal of Archival Studies
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• no.9
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• pp.110-142
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• 2004

This paper treated what are congressional records which are one of core fields of national records and what kind of significant records they have. The characteristics of "substantive records" of the institution among public records are as follows: firstly, their contents depend on the inherent function of the institution; secondly, their types differ by the character of basic unit(member); thirdly, their sources are determined by the character of institution. Starting from the above points of view, the contents, characteristics, main sources, and types by sources of congressional records were presented. They are summarized as follows. In Chapter 2, the substantial records, which document the intrinsic function of congress on the basis of analyses of inherent function and structural uniqueness of congress have natures of which the contents are "legislative records", "oversight records", and "political activity records" starting from the inherency of congress as the people's representation. The typical natures of congress records are related with the specialty that the basic unit of congress structure is an individual congressman as an indepent national institution and congress is a council of these congressmen. Firstly, the records of congressmen as basic member of congress are the national records with the type of personal records. Secondly, "council records" produced by the council (commettee and main conference), which have evidencial and informative value for decision making through the process of investigating, discussing and voting bill and policy (item) of the basis for national management, are very special kind of records, such as item records, decision records, journal records, and congress assistant records. Because congressmen and councils composing congress have an equal inter-relationship in the structure of congress, the main sources of congress records are an individual congressman and all the councils. In chapter 3, the contents and sorts of main records are discribed, centering around congressmen and councils as the main sources of congress records. In chapter 4, the management of records of congressmen is issued as an urgent subject for the management of congress records, instead of conclusions.