• Journal of the Korean Society for Precision Engineering
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• v.16 no.5 s.98
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• pp.104-111
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• 1999

A semi-active suspension system for a vehicle using an Electrorheological Fluid damper has been studied. Apparent viscosity of ERF(Electrorheological Fluid) can be changed rapidly by applying electric field. The damping force of ER damper can be selectively controlled by employing electric field to the ER fluid domain. This paper deals with a two-degree-of-freedom suspension using the ER damper for a quarter car model. An intelligent control method using fuzzy control with genetic algorithm has been employed to control the damping force of the ER damper. The GA designs the optimal structure and performance of Fuzzy Net Controller having hybrid structure. The designed fuzzy net controller has been compared with the skyhook type controller for a quarter car model. The computer simulation results show that the semi-active suspension with ER damper has a good performance in the sense of ride quality with less vibration for ground vehicle.

• Proceedings of the KIEE Conference
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• 1994.07a
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• pp.337-341
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• 1994

This paper describes an effort to develop a simulator of Active Power Filter (APF) by three dimentional(3-D) space current co-ordinate. System current is represented by 3-D vector composed of three current components - active, reactive and distorted. %THD (%Total Harmonics Distortion) can be converted to height-angle of system current vector and power factor can be defined on 3-D space current co-ordinate without loss of generality. Current of APF and power system can be analyzed by 3-D visualization of current vector trajectory. So, the computer simulation results show that the proposed method by 3-D space current co-ordinate make up for disadvantages of performance evaluation on time / frequency domain.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.22 no.12
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• pp.2736-2743
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• 1997

In this paper, the extended finite difference time domain(FDTD) algorithm is applied to carry out full-wave analysis of a microwave amplifier circuit. The active device included in the amplifier is modeled by equivalent current sources. Equivalent current sources are characterizing interaction between electronmagnetic waves and active devices and can be directly incorporated into the FDTD algorithm. To confirm this analysis, an amplifier is implemented. The FDTD simulation shows good agreement with measured results.

• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.27 no.1
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• pp.43-50
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• 2017

Acoustic tiles are typically installed on the surface of pressure vessels in submarines to minimize echoes based on the ship's own noise and active sonar. In this study, we studied low frequency active echo reduction techniques to reduce underwater target echo signals. Active control algorithms using tile type projectors and FxLMS logic have been developed and the projectors have been installed in the assumed hull structure. The effectiveness of projectors and control algorithms has been evaluated in time and frequency domain analysis through experiments in the tank.

• Smart Structures and Systems
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• v.3 no.4
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• pp.405-422
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• 2007

A new hybrid damping technique for vibration reduction in flexible structures, wherein a combination of layers of hard passive damping alloys and active (smart) magnetostrictive material is used to reduce vibrations, is proposed. While most conventional vibration control treatments are based exclusively on either passive or active based systems, this technique aims to combine the advantages of these systems and simultaneously, to overcome the inherent disadvantages in the individual systems. Two types of combined damping systems are idealized and studied here, viz., the Noninteractive system and the Interactive system. Frequency domain studies are carried out to investigate their performance. Finite element simulations using previously developed smart beam elements are carried out on typical metallic and laminated composite cantilever beams treated with hybrid damping. The influence of various parameters like excitation levels, frequency (mode) and control gain on the damping performance is investigated. It is shown that the proposed system could be used effectively to dampen the structural vibration over a wide frequency range. The interaction between the active and passive damping layers is brought out by a comparative study of the combined systems. Illustrative comparisons with 'only passive' and 'only active' damping schemes are also made. The influence and the mode dependence of control gain in a hybrid system is clearly illustrated. This study also demonstrates the significance and the exploitation of strain dependency of passive damping on the overall damping of the hybrid system. Further, the influence of the depthwise location of damping layers in laminated structures is also investigated.

• Structural Engineering and Mechanics
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• v.75 no.2
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• pp.175-191
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• 2020

In this study, multi-story structures are actively controlled using metaheuristic algorithms. The soil conditions such as dense, normal and soft soil are considered under near-fault ground motions consisting of two types of impulsive motions called directivity effect (fault normal component) and the flint step (fault parallel component). In the active tendon-controlled structure, Proportional-Integral-Derivative (PID) type controller optimized by the proposed algorithms was used to achieve a control signal and to produce a corresponding control force. As the novelty of the study, the parameters of PID controller were determined by different metaheuristic algorithms to find the best one for seismic structures. These algorithms are flower pollination algorithm (FPA), teaching learning based optimization (TLBO) and Jaya Algorithm (JA). Furthermore, since the influence of time delay on the structural responses is an important issue for active control systems, it should be considered in the optimization process and time domain analyses. The proposed method was applied for a 15-story structural model and the feasible results were found by limiting the maximum control force for the near-fault records defined in FEMA P-695. Finally, it was determined that the active control using metaheuristic algorithms optimally reduced the structural responses and can be applied for the buildings with the soil-structure interaction (SSI).

• International Journal of Precision Engineering and Manufacturing
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• v.6 no.4
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• pp.37-43
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• 2005

This paper presents a hybrid control algorithm for the active noise control in the rectangular enclosure using an active/passive foam actuator. The hybrid control composes of the adaptive feedforward with feedback loop in which the adaptive feedforward control uses the well-known filtered-x LMS(least mean square) algorithm and the feedback loop consists of the sliding mode controller and observer. The hybrid control has its robustness for both transient and persistent external disturbances and increases the convergence speed due to the reduced variance of the jiltered-x signal by adding the feedback loop. The sliding mode control (SMC) is used to incorporate insensitivity to parameter variations and rejection of disturbances and the observer is used to get the state information in the controller deign. An active/passive smart foam actuator is used to minimize noise actively using an embedded PVDF film driven by an electrical input and passively using an absorption-foam. The error path dynamics is experimentally identified in the form of the auto-regressive and moving-average using the frequency domain identification technique. Experimental results demonstrate the effectiveness of the hybrid control and the feasibility of the smart foam actuator.

• Korean Journal of Veterinary Research
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• v.41 no.2
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• pp.189-195
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• 2001

Single base substitution and deletion mutation have been introducted into the verotoxin 2 (VT2)A subunit gene from O157:H7 isolates to reduce cytotoxicity of VT2 and the cytotoxicity between wild type toxin and mutant toxoid were compared. A M13-derived recombinant plasmid pEP19RF containing a 940bp EcoRI-PstI fragment of VT2A gene was constructed for oligonucleotide-directed mutagenesis. The duoble mutant pDOEX was constructed by point and deletion mutation of two different highly conserved regions of VT2A encoding active site cleft of enzymatic domain. The key residue, Glu 167(GAA) and the pentamer(WGRIS) consisting of the enzymatic domain were replaced by ASP(GAC) and completely deleted in nucleotide sequence analysis of mutant, respectively. In the comparision of vero cell cytotoxicity between wide type toxin and toxoid from mutant, the wild type toxin expressed cytotoxicity in dilution of $10^{-6}$, but the toxid from mutant did not show cytotoxicity to vero cells.

• Journal of Life Science
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• v.18 no.4
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• pp.585-589
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• 2008

Chronophin is a phosphatase responsible for the dephosphorylation of cofilin, which regulates the rearrangement of actin cytoskeleton. It is also known as a phosphatase for pyrodoxal 5'-phosphate (PLP), an active form of vitamin $B_6$, and maintains the level of PLP in the cytoplasm. Since this phosphatase belongs to a HAD subfamily containing a cap domain, it is expected to undergo a conformational change for the binding of a substrate. However, the crystal structure of chronophin has a disulfide bridge between the cap and core domains preventing a movement of the cap domain against the core domain. It is possible that the disulfide bond between C91 and C221 was formed by an oxidation during the crystallization. Here, we obtained chronophin crystals under a reduced condition and determined the crystal structure. This reduced chronophin does not contain a disulfide bridge and shows a closed conformation like the oxidized form. It implies that an active chronophin binds its substrate under the closed conformation without the disulfide bond and shows a high substrate specificity in the cell.

• BMB Reports
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• v.40 no.5
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• pp.783-790
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• 2007

Receptor binding plays an important role in determining host specificity of the Bacillus thuringiensis Cry $\delta$-endotoxins. Mutations in domains II and III have suggested the participation of certain residues in receptor recognition and insect specificity. In the present study, we expressed the cloned domain II-III fragment of Cry4Ba and examined its binding characteristics to mosquito-larval midgut proteins. The 43-kDa Cry4Ba-domain II-III protein over-expressed in Escherichia coli as inclusion bodies was only soluble when carbonate buffer, pH 10.0 was supplemented with 4M urea. After renaturation via stepwise dialysis and subsequent purification, the refolded domain II-III protein, which specifically reacts with anti Cry4Ba-domain III monoclonal antibody, predominantly exists as a $\beta$-sheet structure determined by circular dichroism spectroscopy. In vitro binding analysis to both histological midgut tissue sections and brush border membrane proteins prepared from susceptible Aedes aegypti mosquito-larvae revealed that the isolated Cry4Ba-domain II-III protein showed binding functionality comparable to the 65-kDa full-length active toxin. Altogether, the data present the 43-kDa Cry4Ba fragment comprising domains II and III that was produced in isolation was able to retain its receptor-binding characteristics to the target larval midgut proteins.