• 대한기관식도과학회:학술대회논문집
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• 대한기관식도과학회 1987년도 제21차 학술대회 연제순서 및 초록
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• pp.7.2-7
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• 1987

Waver 와 Bray (1930) 가 cochlear microphonic을 처음 발견한 이래 Derbyshire 와 Davis (1935)는 summating potential을 각각 처음 기술하여 이 세가지 전위를 electrocochleogram 이라 칭하였고 이는 감음신경성 난청의 감별진단 및 청각생리연구에 이용되어 왔다. 저자들은 정상 guinea pig 10 마리를 대상으로 DANAC 7E ERA청각계기를 사용하여 정원창에서 action potential과 summating potential을 측정하였으며 주파수에 따른 역치 자극간 간격 및 두 potential의 상호관계를 분석하여 다음과 같은 결과를 얻었다. 1) 주파수가 증가함에 따라 그 역치는 점차 감소하였다. 2) 자극음의 강도와 action potential의 $N_1$ component 진폭은 상호 비례관계를 보여 주었으며 주파수 증가에 따라 $N_1$ component 진폭은 점차 증가하였다. 3) Action potential 의 $N_1$ component latency는 주파수가 증가할수록 역비례 관계를 보여 주었다. 4) N$_1$ component의 진폭과 자극간 간격(interstimulus interval, ISI)과의 관계는 ISI가 80~160m sec사이에서 plateau를 형성하였다. 5) summating potential은 자극음의 강도가 증가함에 따라 그 진폭이 증가하였으며 action potential도 증가하였으나, SP/AP는 감소하는 경향을 보였다.

• Journal of Chest Surgery
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• 제18권1호
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• pp.1-6
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• 1985

Isolated sinus node cells of the rabbit were used to assess the effects of extracellular Na, K, Ca and Mg concentrations on cardiac pacemaker activity. With intracellular glass micro-electrodes spontaneous action potentials of SA node were recorded and the effects of various ions and their blockers were analyzed in terms of the cycle length, the amplitude and the duration of action potentials, the results obtained were as follows. 1. Sodium reduction [up to 30%] decreased the amplitude of action potential and lengthened the cycle length. TTX, specific blocker of Na channel slightly lengthened the cycle length. 2. Increasing potassium ion concentration, the duration of action potential decreased and the frequency increased in 6mM, however, spontaneous action potential was stopped in 24 mM. Barium ion known to be decreasing K conductance increased the duration of action potential but no significant change in the cycle length was noticed. 3. Calcium ion has shortening effect on the duration and the cycle length of action potential but not with dose-dependent manner. Cadmium ion .[0.02mM] lengthened cycle length and the duration of action potential. 4. Increasing the concentration of magnesium ion the cycle length was lengthened, significantly.

• The Korean Journal of Physiology
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• 제9권2호
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• pp.41-48
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• 1975

The failure of the action potential at the end-plate membrane to reach the sodium equilibrium potential is due to the stimulating action of acetylcholine on $Na^+-K^+$ pump. This action of acetylcholine causes an enormous increase in the $K^+$ transport rate. The quantitative amount of potassium ions in the inside of the end-plate membrane prevented the permeability of sodium ions during the depolarization phase of the action potential. It would favor the changes in the shape of action potential by acetylcholine which are always toward a fixed potential slightly below the zero line. The increased $Na^+-K^+$ pump activity by acetylcholine is responsible for the hypopolarization of membrane. This reduces the membrane resistance of the end-plate during transmitter activity.

• 동의생리병리학회지
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• 제27권5호
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• pp.563-569
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• 2013

This study was undertaken to apply the yin-yang theory in action potential. In order to apply the yin-yang theory in action potential, nature of yin and yang, interrelation of yin and yang and action potential in cell were reviewed. According to the yin-yang theory, inner cellular space corresponds to yin, but outer cellular space corresponds to yang. If we classify ions in intracellular fluid or extracellular fluid by nature of yin and yang, potassium(K+) corresponds to yang within yin(陰中之陽), protein(Pr-) corresponds to yin within yin(陰中之陰) in intracellular fluid, and sodium(Na+) corresponds to yang within yang(陽中之陽), chloride(Cl-) corresponds to yin within yang(陽中之陰) in extracellular fluid. Double donnan equilibrium and equilibrium potential were caused by intracellular anion(Pr-) and extracellular cation(Na+) are related with mutual rooting of yin and yang(陰陽互根) and opposition of yin and yang(陰陽對立). The influx and efflux of ion through cell membrane means waxing and waning of yin and yang(陰陽消長), the change of membrane potential means yin-yang conversion(陰陽轉化) during action potential.

• The Korean Journal of Physiology
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• 제16권2호
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• pp.119-128
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• 1982

The frog truncus arterious were studied with conventional glass microelectrode technique in order to elucidate the underlying mechanism of spontaneous pacemaker activity. The analyses were focussed on the ionic nature of pacemaker current by changing the concentrations of extracellular $K^+$ and, $Na^+$, or by using blockers of K- and Ca-current and chronotropic transmitters. 1) The action potential of the spontaneously active truncus arteriosus has some characteristic feature of maximal distolic potential ranged from -65 to -75 mV, resting potential from -45 to -50 mV and overshoot voltage about +30 mV, respectively. Duration of the action potential taken from rapid upstroke to maximal diastolic potential was about 600 msec. Usual discharge rate was $25{\sim}30/min$ at room temperature $(18{\sim}20^{\circ}C)$. 2) The sensitivity of the resting membrane potential to change extracellular potassium concentrations $(0{\sim}12\;mM)$ was relatively low. Transient hyperpolarization was appeared in the 12 mM K Ringer after 10 min exposure to 0 mM K and it could be related to Na-pump reactivation by high potassium. 3) Reduction of extracellular sodium concetrations diminished the amplitude and frequency of the action potential. In Ringer solution containing 30% Na (substituted by equimolar Tris), spontaneous activity stopped but reappeared as very slow and small action potential. There was no spotaneous activity in zero Na Ringer solution. 4) Caesium(10 mM), K-current blocker decreased the frequency of the action potential and also pacemaker depolarization. Manganese (2 mM) known to be Ca-current antagonist, blocked spontaneous activity completely. 5) Adrenaline and acetylcholine had no chronotropic effect. But adrenaline increased the duration of plateau phase and the magnitude of the action potential in the follower cell. It is concluded that K-, Na-and Ca-current components are involved in the genesis of spontaneous activity of the frog truncus arteriosus like cardiac pacemaker tissues. But the insensitivity of truncus arteriosus to adrenaline and acetylcholine indicates that there are some different control mechanisms of spontaneous rhythm in two tissues.

• The Korean Journal of Physiology
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• 제22권2호
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• pp.207-218
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• 1988

Effects of 1, 4-dihydropyridine compounds, such as nifedipine, nisoldipine, nitrendipine, and nimodipine which were calcium antagonists on the normal and Ca-dependent, slow channel mediated action potentials in the guinea pig's papillary muscle were investigated. The glass microelectrode was impaled into a papillary muscle cell for measurements of potential changes with the simultaneous tracing of isometric contraction. The concentration of Ca antagonists were 1 mg/l (nifedipine and nisoldipine), 2 mg/l (nitrendipine and nimodipine), which showed the maximal inhibition of isometric contraction (above 90%) and simultaneous effects on the normal action potentials and only the halves of those concentrations were sufficient to observe the effects on the calcium action potentials. The data for analysis were only chosen when the microelectrode was maintained in a cell throughout the experiments. 1, 4-Dihydropyridine compounds decreased the action potential duration but did not affect the resting membrane potential, overshoot, and upstroke velocity of the normal action potentials with the decrease in the isometric contraction. And with the decrease in the area and amplitude of isometric contraction, the area, amplitude, upstroke velocity and duration of Ca action potential was decreased. But the differences in the effects of the Ca antagonists were not observed. Therefore it is inferred that the changes in normal and Ca action potential induced by the 1, 4-dihydropyridine compounds with a common chemical structure would be caused by the slow inward Ca-current, not by a fast Na-current.

• The Korean Journal of Physiology
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• 제15권1호
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• pp.3-8
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• 1981

Studies have been conducted to test the effect of Ginseng alcohol extract on the membrane potentials of frog skeletal muscle. The gastrocnemius muscle was isolated and placed in a chamber containing the Clark-frog Ringer solution. Membrane potentials were recorded using microelectrodes filled with 3 M KCI and muscle was electrically stimulated to obtain action potential. Changes in both the action potential and the resting membrane potential were observed after adding an appropriate amount of Ginseng alcohol extract in the perfusing Ringer solution. The results obtained from 346 muscle cells are summarized as follows : 1) The average resting membrane potential of the normal frog gastrocnemius muscle cell was -92.8 mV and the peak of the action potential reached at 29.8 mV. 2) Both the resting membrane potential and the peak of the action potential decreased by Ginseng alcohol extract, the effect being proportional to the dose of Ginseng alcohol extract. 3) The resting membrane potential and the peak of the action potential continuously decreased until about 40 min after Ginseng addition and leveled off thereafter. The potentials recovered to its original value after Ginseng was washed out. 4) The resting membrane potential was more sensitive to the Ginseng alcohol extract than was the action potential. These results strongly suggest that Ginseng alcohol extract increases both the $Na^+$ and $K^+$ permeability in the skeletal muscle cell membrane.

• The Korean Journal of Physiology
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• 제23권2호
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• pp.339-350
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• 1989

Electrophysiological effects of ammonia was studied in the isolated superfused sinus node and atrial muscle cells of the rabbit heart. No significant changes were observed in the overshoot potential (05), maximum diastolic potential (MDP), and action potential amplitude (APA) of the sinus node cells following superfusion with 3.0 mM ammonia, fifty times upper limit of the normal human plasma level. However the action potential duration (APD) of sinus node cells were significantly prolonged after superfusion with 0.6 mM ammonia for 20 min or with 1.2 and 3.0 mM ammonia for 5 minutes. Ammonia in all the concentrations tested decreased the rate of spontaneous firing (RSF) from the sinus node cells. After superfusion of sinus node cells with 0.3 mM ammonia for 20 min, the RSF significantly decreased from 20 min to 25 min after onset of superfusion while a significant decrement in the RSF was observed from 7 min to 30 min following superfusion with 3.0 mM ammonia for S min. On the other hand, the effects of ammonia on the action potential of the rabbit atrial muscle cell were much similar to those on pacemaker cells except that the atrial cell was generally less sensitive to ammonia. The results suggest that ammonia may cause changes in the action potential of the rabbit cardiac cells by the direct action, and that the cardiac effects of ammonia are generally opposite to those of glycine.

• 약학회지
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• 제32권6호
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• pp.402-414
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• 1988

In order to clarify the receptor types and mechanisms underlying the positive inotropic effect of dopamine on the mammalian ventricular myocardium, the action potential, its first derivatives and isometric contraction of the rabbit papillary muscle were recorded using a force transducer and glass capillary microelectrodes filled with 3M KCl. The results were as follows; (1) In normal Tyrode solution, the contractile force was increased and duration of action potential was shortened with increments of dopamine concentration ($10^{-6}-10^{-4}M$). (2) The dose-response curve was markedly shifted to the right by pretreatment with reserpine (5mg/kg i.p., 24hrs prior to the experiment). (3) In 19mM $K^+-Tyrode$ solution, the duration of action potential, maximum rate of rise (V_{max}) of action potential and overshoot were significantly increased with increments of dopamine concentration ($10^{-6}-10^{-4}M$). (4) The inotropic effect of dopamine on the rabbit papillary muscle pretreated with reserpine was antagonized by atenolol ($10^{-6}M$), but not by phentolamine ($3{\times}10^{-6}M$). (5) In rabbit papillary muscle partially depolarized by 19mM $K^+-Tyrode$ solution, slow electrical response (calcium mediated action potential) as well as contraction were restored by dopamine ($10^{-4}M$); this restoration was blocked by calcium antagonists ($3{\times}10^{-5}M$ $LaCl_3{\cdot}6H_2O$, $3{\times}10^{-6}M$ diltiazem) or ${\beta}-adrenoceptor$ antagonist ($3{\times}10^{-6}M$ atenolol), but not affected by ${\alpha}-adrenoceptor$ antagonist ($10^{-5}M$ phentolamine, $3{\times}10^{-6}M$ yohimbine) or vascular dopaminergic receptor antagonist ($10^{-5}M$ haloperidol). The above results may be interpreted as that the positive inotropic effect of dopamine through both direct and indirect action are caused by increase in slow inward current ($Ca^{2+}$ influx into themyocardial cell), and the direct action is mainly due to the stimulation of ${\beta}-adrenoceptors$ in the rabbit papillary muscle.

• The Journal of Korean Physical Therapy
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• 제15권4호
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• pp.455-465
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• 2003

The purpose of this study was to describe the alteration of muscle action potential of spastic upper extremity in a patient with cerebral palsy by the water temperature. We used seven patients with cerebral palsy. Participants classified according to each group in $29^{\circ}C,\;35^{\circ}C$ by the water temperature. All subjects participated 2 session, which at least 1 week between session. The test was measured continuously pre test, post-test by thermometer and surface EMG. The following results were obtained; 1. In changes of surface temperature, wrist flexor and extensor were significantly decreased in $29^{\circ}C$ (p<0.001) group but were significantly increased $35^{\circ}C$ group(p<0.01). 2. In changes of surface temperature, wrist flexor and extensor were significantly differenced between $29^{\circ}C$ and $35^{\circ}C$ group(p<0.001). 3. In changes of muscle action potential, wrist extension antagonist were significantly increased in $29^{\circ}C$ group(p<0.05). 4. In changes of muscle action potential, wrist flexion agonist were more significantly increased in $29^{\circ}C$ group(p<0.01). 5. In changes of muscle action potential, wrist extension antagonist were significantly differenced between $29^{\circ}C$ and $35^{\circ}C$ group(p<0.05). These results lead us to the conclusion that changes of muscle action potential of spastic upper extremity in a patient with cerebral palsy were influenced by the water temperature. Therefore, a further direction of this study will be to provide more evidence that a moderate water temperature have an effect on muscle tone in a patient with cerebral palsy.