• CELLMED
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• v.4 no.1
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• pp.5.1-5.8
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• 2014

Healthcare-associated infection represents a frequent cause of mortality that increases hospital costs. Due to increasing microbial resistance to antibiotics, it is necessary to search for alternative therapies. Consequently, novel alternatives for the control of resistant microorganisms have been studied. Among them, plant antimicrobial protein presents enormous potential, with flowers being a new source of antimicrobial molecules. In this work, the antimicrobial activity of protein-rich fractions from flower tissues from 18 different species was evaluated against several human pathogenic bacteria. The results showed that protein-rich fractions of 12 species were able to control bacterial development. Due its broad inhibition spectrum and high antibacterial activity, the protein-rich fraction of Hibiscus rosa-sinensis was subjected to DEAE-Sepharose chromatography, yielding a retained fraction and a non-retained fraction. The retained fraction inhibits 29.5% of Klebsiella pneumoniae growth, and the non-retained fraction showed 31.5% of growth inhibition against the same bacteria. The protein profile of the chromatography fractions was analyzed by using SDS-PAGE, revealing the presence of two major protein bands in the retained fraction, of 20 and 15 kDa. The results indicate that medicinal plants have the biotechnological potential to increase knowledge about antimicrobial protein structure and action mechanisms, assisting in the rational design of antimicrobial compounds for the development of new antibiotic drugs.

• Journal of Korean Society of Forest Science
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• v.81 no.4
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• pp.372-382
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• 1992

Recreation planning is essential activity to meet changing demands and to protect the resources. The recreation opportunity specturm(ROS) system is a principal part of a recreational management planning. In this study, the basic concepts and tenets of the ROS system described and reviewed the feasibility of applying to forest recreation planning to the Korean national forest. In Korea, the forest land as a major recreation place has used without the rational planning process. The control for the laissez-faire use on the forest area, the classification of recreational opportunity settings is the most important process and then it make a useful tool for providing proper recreational opportunity and site development guidance. Opportunity settings classification can help maintain diversity and enhance protection of forest resources. It can also improve the quality of recreational experiences and the management action guidances. GIS technology using the ARC/INFO could be useful in current attempts to identify analysis areas for predictive modeling of forest recreation site planning.

• The Korean Journal of Pesticide Science
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• v.8 no.2
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• pp.79-87
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• 2004

3-Chloro-2-[4-chloro-2-fluoro-5-(5-methyl-3-phenyl-4,5-dihydroisoxazol-5-ylmethoxy)-phenyl]-4,5,6,7-tetrahy dro-2H-indazole(EK-5439) demonstrated rice selectivity and herbicidal activity on annual weeds, such as Echinochloa oryzicola, Monochoria vaginalis, Lindernia pyxidaria, Rotala indica, Aneilema keisak, Cyperus difformis, and Ludwigia prostrata at doses of 16-63 g a.i./ha. However, the application window was limited from pre-emergence to 5 days after transplanting. The control efficacy of EK-5439 on barnyardgrass was 4 times higher than that of oxadiazon. EK-5439 was excellently safe to the 16 different transplanted rice cultivars treated 2 days after transplanting. These compounds have the mechanism of action on the chlorophyll biosynthesis like protoporphyrinogen IX oxidase inhibitors.

• Weed & Turfgrass Science
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• v.2 no.3
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• pp.221-229
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• 2013

This paper reviews current status of weed control practices in herbicide-resistant crops to examine weed management strategies in cope with cropping herbicide-resistant crops in the near future. Herbicide-resistant crops were rapidly adopted weed management technologies due to broad-spectrum weed control without crop injury. Transgenic glyphosate-resistant cultivars in soybean, corn, canola, and cotton were adopted to manage weeds at lower cost in a simplified weed management system. Dual stack crops with glyphosate and glufosinate resistance were developed to control glyphosate resistant weeds in corn, soybean and cotton. New multiple herbicide-resistant crops with resistance to glyphosate and glufosinate, acetolactate synthase (ALS) inhibitors, synthetic auxin herbicides, 4-hydroxyphenyl pyruvate dioxygenase (HPPD) inhibitors or acetyl Coenzyme A carboxylase (ACCase) inhibitors will expended the utility of existing herbicide technologies to manage the evolution of resistant weeds. However, herbicide resistant crops alone cannot solve weed problems and thus studies on diverse weed managements using an array of alternating herbicides of mode of action, mechanical, and cultural practices are needed for integrated weed management systems in the future.

• Microbiology and Biotechnology Letters
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• v.38 no.1
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• pp.64-69
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• 2010

It has been known that propolis possesses anti-infective, anti-inflammatory, and anti-oxidative properties. Although antifungal activity of Propolis has already been demonstrated, very few studies has been conducted for action mechanism and its spectrum on fungi. We found that ethanol extract of propolis (EEP) inhibited in vitro translation. Since we also observed the growth inhibition of pathogenic fungi and anti-oxidative properties preliminarily, we try to see where those properties come from. Therefore we extracted the EEP further with chloroform, ethyl acetate and butanol. When their fractions were examined for the growth inhibition of Candida albicans, Saccharomyces cerevisiae, Candida glabrata, Candida lusitaniae, Cryptococcos neoformans, chloroform fraction exhibited the highest anti-fungal as well as anti-oxidative properties. Similarly the chloroform fraction showed highest translation-inhibiting activities among the various Propolis fractions. These data indicate that those properties might come from similar compounds.

• The Korean Journal of Pesticide Science
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• v.6 no.3
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• pp.183-192
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• 2002

Herbicidal effect of KSC-35089, a newly synthesized N-arylphthalimide compound, was examined, and its interaction with quizalofop-P-ethyl when foliar applied as a tank-mixture was evaluated under a greenhouse condition. KSC-35089 showed stronger herbicidal activity on broadleaves than grasses, suggesting that it would be an appropriate partner for non-selective weed control with quizalofop-P-ethyl, a strong grasskilling ageant. In general, the interaction between KSC-35089 and quizalofop-P-ethyl appeared to be additive, which indicates each compound acts on whole plant level independently. This additive interaction was confirmed in a spectrum test using 19 grasses and 26 broadleaves. Under the greenhouse condition, all tested plants were controlled around 90% level through a foliar application with a tank-mixture of KSC-35089 at 64 g ai/ha and quizalofop-P-ethyl at 32 g ai/ha. Further study would be required on the action mechanism of this additive interaction.

• Korean Journal of Weed Science
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• v.7 no.2
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• pp.236-242
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• 1987

The study was conducted to interprete the fluctuation of weed vegetation in plant-sociological impacts as affected by the mixture use of oxyfluorfen and glyphosate with various dosages. Also, intended to know the real interaction between two herbicides in weeding efficacies. The better efficacy from the above mixture was recognized than from the oxyfluorfen + paraquat mixture on the perennial-sites. In lower rate mixture of oxyfluorfen, the dominance index was increased by the annual grass species (ie. Digitaria), and of glyphosate by the biennial Stellaria and perennial species (ie. Artemisia). Also, the positive maximum action of both oxyfluorfen and glyphosate in various mixture rates was categorized upto 0.55kg ai/ha for oxyfluorfen and 0.35kg ai/ha for glyphosate, respectively. However, the interaction between the above two herbicides recognized actually as negative. Consequently, the use of mixture compound of oxyfluorfen with glyphosate are expected rather to promote the control efficacy of specific weed species, to enlarge the weeding spectrum and to prolong the weeding periods than to reduce the application rate of both chemicals depending on any synergic interactions.

• Korean Journal of Medicinal Crop Science
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• v.28 no.4
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• pp.276-286
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• 2020

Background: Non-alcoholic fatty liver disease (NAFLD) is a chronic liver disease associated with multiple metabolic disorders. The medicinal plant Curcuma longa L. is widely distributed in Asia and has been used to treat a spectrum diseases in clinical practice. To date, there are inadequate reports of the effects of C. longa 50% EtOH extract (CE) on NAFLD. Therefore, in this study, we evaluate the CE on an NAFLD animal and elucidate the mechanism of action. Methods and Results: C57BL/6J mice fed a methionine-choline deficient diet (MCD) were treated with CE or milk thistle, and changes in inflammation and stetosis were assessed. Experimental animals were divided into six group (n = 10); Normal, MCD, MCD + CE 50 mg/kg/day (CE 50), MCD + CE 100 mg/kg/day (CE 100), MCD + CE 150 mg/kg/day (CE 150), and the Control, MCD + Milk thistle 150 mg/kg/day (MT 150). Body weight, liver weight, liver function, and histological changes were assessed in experimental animals. Quantitative real-time polymerase chain reaction and western blot analyses were performed on samples collected after 4 weeks of treatment. We observed that CE administration improved MCD-diet-induced lipid accumulation, and triglyceride (TG) and total cholesterol (TC) levels in serum. Treatment with CE also decreased hepatic lipogenesis through modulation of the sterol regulatory element binding protein-1 (SREBP-1), CCAAT-enhancer binding protein α (C/EBPα), fatty acid synthase (FAS), and peroxisome proliferator-activated receptor γ (PPARγ) expresion. In addition, the use of CE increased adenosine monophosphate-activated protein kinase (AMPK) phosphorylation and inhibited the up-regulation of toll-like receptor (TLR)-2 and TLR-4 signaling and the production of inflammatory mediators. Conclusions: In this report, we observed that CE regulated lipid accumulation in an MCD dietinduced NAFLD model by decreasing lipogenesis. These data suggeste that CE could effectively protect mice against MCD-induced NAFLD, by inhibiting the TLR-2 and TLR-4 signaling cascades.

• Korean Journal of Food Science and Technology
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• v.33 no.5
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• pp.611-618
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• 2001

The ethanol extract of Dryopteris crassirhizoma Nakai showed strong growth inhibition against 5 strains of Listeria monocytogenes at the concentrations of $100{\sim}500$ ppm and the minimum inhibitory concentration of n-hexane fraction was under 50 ppm. The D8-2-5 fraction isolated from n-hexane fraction of Dryopteris crassirhizoma Nakai showed a strong bactericidal activity on 5 strains of L. monocytogenes at 20 ppm level in tryptic soy broth medium. At the level, the viable count was reduced $4{\sim}6$ log cycle compared to initial cell number. Observation by the measurement of adenosine triphosphate (ATP) contents and transmission electron microscope showed that disruptions of the cell wall and elution of intracellular ATP are assumed to be due to the bactericidal activity. In addition, the n-hexane fraction of Dryopteris crassirhizoma Nakai showed the strong growth inhibitions at 50 ppm on Vibrio parahaemolyticus and Bacillus cereus, and at 25 ppm on Staphylococcus aureus.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.32 no.6
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• pp.531-552
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• 2020

In the previous study, both the wave characteristics at the tip of composite breakwater and on caisson were investigated by applying olaFlow numerical model of three-dimensional regular waves. In this paper, the same numerical model and layout/shape of composite breakwater as applied the previous study under the action of one directional irregular waves were used to analyze two and three-dimensional spatial change of wave force including the impulsive breaking wave pressure applied to trunk of breakwater, the effect of rear region, and the occurrence of diffracted waves at the tip of caisson located on the high crested rubble mound. In addition, the frequency spectrum, mean significant wave height, mean horizontal velocity, and mean turbulent kinetic energy through the numerical analysis were studied. In conclusion, the larger wave pressure occurs at the front wall of caisson around the still water level than the original design conditions when it generates the shock-crushing wave pressure in three-dimensional analysis condition. Which was not occurred by two-dimensional analysis. Furthermore, it was confirmed that the wave pressure distribution at the caisson changes along the length of breakwater when the same significant incident wave was applied to the caisson. Although there is difference in magnitude, but its variation shows the similar tendency with the case of previous study.