• Journal of Microbiology and Biotechnology
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• 제27권11호
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• pp.1932-1941
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• 2017

DFC-2, a methyl 5-[2-(dimethylamino)ethoxy]-7,12-dioxo-7,12-dihydrodinaphtho[1,2-b:2',3'-d]furan-6-carboxylate, is reported to have antitubercular effects against Mycobacterium tuberculosis. At concentrations ranging from 0.19 to $0.39{\mu}g/ml$, DFC-2 inhibited both drugusceptible and -resistant strains of M. tuberculosis. Microarray analyses were employed to gain insights into the molecular mechanisms of DFC-2's action in M. tuberculosis. The most affected functional gene category was "lipid biosynthesis," which is involved in mycolic acid synthesis. The decrease in transcription of genes related to mycolic acid synthesis was confirmed by RT-PCR. Furthermore, we found that DFC-2 triggered a reduction in mycolic acid levels, showing a similar pattern to that of mycolic acid synthesis inhibitor isoniazid. These results may explain how this compound kills mycobacteria efficiently by inhibiting mycolic acid synthesis.

• 한국의류학회지
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• 제34권5호
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• pp.819-830
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• 2010

This study proposes medical gowns for interns and residents that address the functions of utility and activity. Based on the questionnaires, the prototype focused on the pockets for practical use and movement adaptability. Functional pockets were made by increasing pocket sizes and separating pockets with sections according to use. An inside pocket and a loop for an identification tag was placed on the chest pocket and the side seam pocket were oblique lined. To resolve horizontal stress on the back of the gown,6 cm action pleats were added at both ends of the bladebone. The elbow area was tucked up to improve the workplace activities. To reduce hamper on the gown hemline, slits were added along the gown side seam. The front button was changed into an inside button closure. The cuffs, belt, and back waist belt were removed due to occupational cases of inconvenience. The length of the sleeves was adjusted with belts to improve the movement of the arms. In accordance with design preference, the gown was designed to be knee-length with wrist length sleeves. Blue colored (2.5PB 3/12: by Munsell) material was inserted to reduce the contamination on the neck, cuffs, front closure, and hemline area. The planning cloth of the experimental gown was designed to improve activity function, cleaning, and comfort from light materials. The field test (with 30 doctors at university hospitals and the objective assessment with 9 subject groups)were done by the valuation between the existing gown and the prototype. The results showed that the prototype had to be modified by decreasing the pocket size, expanding the usage of the penholder, and simplify front pockets in order to maintain unity. The loop for the identification tag, inside pocket, and the blue colored material had to be removed. The front closure of the gown had to return to its original state.

• 대한한방내과학회지
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• 제29권1호
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• pp.243-257
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• 2008

Myostatin, a negative regulator of myogenesis, is shown to function by controlling the proliferation of myoblasts. In this study we show that myostatin is an inhibitor of myoblast differentiation and that this inhibition is mediated through Smad 3. To determine MyoD expression by Dodamtang treatment, we compared the expression pattern of $C_{2}C_{12}$ mouse myoblasts that constitutively express myostatin with control cells. In vitro, increasing concentrations of Dodamtang reversibly prevented the myogenic blockage of myoblasts by myostatin expression. ELISA assay, Western and confocal analysis indicated that treatment of Dodamtang to the low serum culture media increased the levels of MyoD leading to the inhibition of myogenic differentiation by myostatin. The stable transfection of $C_{2}C_{12}$ myoblasts with myostatin expressing constructs did rescue MyoD-induced myogenic differentiation. Consistent with this, the treatment of Dodamtang rescued the expression of a MyoD in $C_{2}C_{12}$ myoblasts treated with myostatin. Taken together, these results suggest that induction of MyoD by Dodamtang inhibits myostatin activity and expression via SMAD3 resulting in the rescue of the myoblasts to differentiate into myotubes. Thus we propose that myostatin action by Dodamtang plays a critical role in myogenic differentiation and that the muscular hyperplasia and hypertrophy seen in animals that blockage of functional myostatin is because of deregulated proliferation and differentiation of myoblasts.

• 패션비즈니스
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• 제6권5호
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• pp.27-40
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• 2002

The purpose of this thesis is presenting desirable design of uniform which could reflect high school girls' demands with the researches on their consciousness of fashion and actual state of uniforms. Methods to accomplish this research include first, examinationson characteristics of high school girls and their consciousness of fashion with relevant articles, second, interviews with designers from major domestic uniform brands - ELITE, IVY, SMART - and third, analysis on related internet sites. Practical researches are followed by design analysis and proposal of improvement on high school girls' uniform, accompanied by collection of photographs from field studies, major uniform brands' shops and uniform related internet sites. Korean high school girls in the digital era are able to express themselves in each different fashion with own individuality, preference or intention, as they are particularly interested in clothes or their appearances. Their consciousness of fashion became visible in a shape of negative attitude and dissatisfaction with uniforms due to its out-of-date design, lack of features and inconvenience in action. Besides more than half of high school girls already experienced reforming it for better looks. As a result of current analysis on references of uniform designs, various levels of refinement were noticed even in uniform of similar details, design, fabric textile and color due to differences on size & length of jacket collar, dart, cutting line, size of details, decorative line, easiness in fit, length of clothes, and etc. To provide students of more refined and desired designs which reflect their demands, current uniforms are to begin acquiring delicate changes with present vogue and feature such as size of details, silhouette, cutting line, decorative line and etc. In addition newly designed uniforms are to present desirable design with image of refinement and neat as they actively comprehend students' needs within its symbolic, functional, economic and aesthetic characteristics by nature.

• 공업화학
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• 제25권6호
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• pp.607-612
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• 2014

이산화지르코늄 표면에 흡착되는 금 입자의 분포 또는 그 반대 경우의 분포에 영향을 끼칠 수도 있는 정전기적 상호작용과 금 입자를 코팅한 mercaptoundecanoic acid층의 표면물성을 규명하였다. 이를 위하여, 원자힘현미경(AFM)으로 mercaptoundecanoic acid층 표면과 이산화지르코늄표면 사이의 표면힘을 염 농도와 pH 값에 따라 측정하였다. 측정된 힘은 Derjaguin-Landau-Verwey-overbeek (DLVO) 이론에 의해 표면의 정량적인 전하밀도와 포텐셜 값들로 전환되었다. 이 값들이 염 농도와 pH에 따라 달라지는 특성을 질량보존의 법칙으로 기술하였으며, 산출된 표면 특성의 염 농도 의존성은 이론적으로 예측했던 결과와 일치하는 것으로 확인되었다. Mercaptoundecanoic acid층의 표면이 이산화지르코늄 표면보다 높은 전하밀도와 포텐셜을 갖는 것이 발견되었는데, 이는 mercaptoundecanoic acid층의 이온화 기능기에 기인한 것으로 생각된다.

• The Korean Journal of Physiology and Pharmacology
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• 제7권1호
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• pp.15-23
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• 2003

Cellular redox state is known to be perturbed during ischemia and that $Ca^{2+}$ and $K^2$ channels have been shown to have functional thiol groups. In this study, the properties of thiol redox modulation of the ATP-sensitive $K^2$ ($K_{ATP}$) channel were examined in rabbit ventricular myocytes. Rabbit ventricular myocytes were isolated using a Langendorff column for coronary perfusion and collagenase. Single-channel currents were measured in excised membrane patch configuration of patch-clamp technique. The thiol oxidizing agent 5,5'-dithio-bis-(2-nitro-benzoic acid) (DTNB) inhibited the channel activity, and the inhibitory effect of DTNB was reversed by dithiothreitol (disulfide reducing agent; DTT). DTT itself did not have any effect on the channel activity. However, in the patches excised from the metabolically compromised cells, DTT increased the channel activity. DTT had no effect on the inhibitory action by ATP, showing that thiol oxidation was not involved in the blocking mechanism of ATP. There were no statistical difference in the single channel conductance for the oxidized and reduced states of the channel. Analysis of the open and closed time distributions showed that DTNB had no effect on open and closed time distributions shorter than 4 ms. On the other hand, DTNB decreased the life time of bursts and increased the interburst interval. N-ethylmaleimide (NEM), a substance that reacts with thiol groups of cystein residues in proteins, induced irreversible closure of the channel. The thiol oxidizing agents (DTNB, NEM) inhibited of the $K_{ATP}$ channel only, when added to the cytoplasmic side. The results suggested that metabolism-induced changes in the thiol redox can also modulate $K_{ATP}$ channel activity and that a modulatory site of thiol redox may be located on the cytoplasmic side of the $K_{ATP}$ channel in rabbit ventricular myocytes.

• 한국생물물리학회:학술대회논문집
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• 한국생물물리학회 2003년도 정기총회 및 학술발표회
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• pp.42-42
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• 2003

Large-conductance $Ca^{2+}$-activated potassium channels ($BK_{Ca}$ are a widely distributed and play key roles in various cell functions. In nerve cells, $BK_{Ca}$ channels shorten the duration of action potentials and block $Ca^{2+}$ entry thereby repolarizing excitable cells after excitation. $BK_{Ca}$ channel opening has been postulated to confer neuroprotection during stroke, and has attracted attention as a means for therapeutic intervention in asthma, hypertension, convulsions, and traumatic brain injury. Several natural and synthetic compounds including a steroid hormone, $\beta$-estradiol, have been identified as the activators of $BK_{Ca}$ channels. Based on the structural features of the previously reported activators of $BK_{Ca}$ channels, we designed several lead compounds, synthesized chemically, and tested their functional activity on cloned $BK_{Ca}$ channels. The $\alpha$ subunit of rat $BK_{Ca}$ channel was expressed alone or with different $\beta$ subunits in Xenopus oocytes and the effects of the compounds were tested electrophysiological means. One of the lead compounds affected the activity of the $\alpha$ subunit of $BK_{Ca}$ channel in a $\beta$ subunit-specific manner. While the activity of B $K_{ca}$ channel $\alpha$ subunit was Potentiated, the channel composed of $\alpha$ and $\beta$1 subunits were inhibited by this compound. We are currently investigating the mechanism of the $\beta$ subunit-dependent effects and planning to localize the receptor site of the lead compound.f the lead compound.

• 동아시아식생활학회지
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• 제14권5호
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• pp.425-437
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• 2004

Medicinal plants are of great importance in providing healthcare to a large portion of the population in Korea. A number of plants are described in Dong-Ui-Bo-Gam for use in the treatment of allergic disorders, namely psoriasis, eczema, bronchial asthma, etc. In this study, we evaluated the effect of AllerQ, which is multi-complexes of various plants extracts such like Mori folium, Scutellaria baicallensis, Glycyrrhiza uralnsis, Mentha sacharinensis and Poncirus trifoliata on compound 48/80 induced anaphylactic shock, ovalbumin induced asthma in vivo and anti-IgE antibody induced hypersensitivity in vitro. We found antianaphylactic or antiallergic properties of AllerQ when given orally. AllerQ for prophylactic treatment for anaphylactic shocks have produced good results. AllerQ may modulate various aspects of immune function and allergic inflammation. In the present study, we analyse the effects of AllerQ on mast cell degranulation, mortality, cAMP/cGMP, O₂, H₂O₂ level, cyokine production and on the elicitation of IgE-mediated mast cell-dependent allergic inflammation in vivo and in vitro. We have established that AllerQ inhibited histamine release, cAMP/cGMP, O₂, H₂O₂ level, IL-4, tumor necrosis factor-alpha(TNF-α) and IL-6 production without having any significant physical change. These effects have been observed in mast cell(in vitro) and serum(in vivo) derived from three different origins that were activated by either immunological or non-immunological stimuli. These results suggest that the antianaphylactic and antiasthma tic action of AllerQ may be associated with an increase in the intracellular inhibition of the cAMP phosphodiesterase. Furthermore, AllerQ identified as potent inhibitors on O₂, H₂O₂ and cytokine activity. these data suggest that AllerQ may have an inhibitory role in mast cell-mediated allergic inflammation, and thus might be considered as an useful functional food.

• Korean Chemical Engineering Research
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• 제50권2호
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• pp.379-384
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• 2012

이산화티탄 표면에 흡착되는 금 입자의 분포 또는 그 반대 경우의 분포에 영향을 끼칠 수도 있는 정전기적 상호작용과 금 입자를 코팅한 Glutathione 층의 표면물성을 규명하였다. 이를 위하여, 원자힘현미경(AFM)으로 Glutathione 층표면과 이산화티탄표면 사이의 표면힘을 염 농도와 pH 값에 따라 측정하였다. 측정된 힘은 Derjaguin-Landau-Verwey-Overbeek(DLVO) 이론으로 해석되어 표면의 정전기적인 특성들이 정량적으로 산출되었다. 이 특성들이 염 농도와 pH에 대하여 나타내는 의존성을 질량보존의 법칙으로 기술하였다. pH 8과 11에서 실험으로 산출된 표면 특성의 염 농도 의존성은 이론적으로 예측했던 결과와 일치하는 것으로 관찰되었다. pH 8과 11에서 Glutathione 층의 표면이 이산화티탄 표면보다 높은 정전기적 특성을 갖는 것이 발견되었는데, 이는 Glutathione 층의 이온화-기능-그룹에 기인한 것으로 생각된다.

• Korean Chemical Engineering Research
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• 제56권5호
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• pp.625-630
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• 2018

지르코니아와 금 표면 위에 형성된 메르캡토언데실인산층 사이에서 정전기적 상호작용이 규명되었다. 이를 위하여, 원자힘현미경(AFM)이 표면들 사이에서 pH값과 염 농도에 따라 작용하는 힘의 측정에 이용되었다. 측정된 힘은 Derjaguin-Landau-Verwey-Overbeek (DLVO) 이론으로 해석되어 각 조건에 대해서 표면의 정전기적인 특성들이 정량적으로 산출되었다. 이 표면 특성들의 염 농도와 pH에 대한 의존성이 질량보존의 법칙으로 예측된 결과와 일치하였다. pH 의존성은 표면 위의 이온화 기능기들로 설명될 수 있다. pH 4와 8에서 메르캡토언데실인산층이 지르코니아보다 더 많은 표면전하밀도와 전위차를 가지는 것은 그 층의 이온화 기능기들에 기인한 것으로 생각된다.