• Proceedings of the Korean Radioactive Waste Society Conference
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• 2004.06a
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• pp.344-344
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• 2004

한국원자력연구소에서는 사용후핵연료의 체적을 감소시켜 저장 안전성 및 경제성을 확보키 위한 사용후핵연료 차세대관리 공정(ACP)을 개발하고 있다. 이 기술의 개발을 위해서는 사용후핵연료를 사용한 실증시험이 필수적이며, 이를 위한 ${\alpha}-{\gamma}$ type의 hot cell 시설 및 부속시설이 필요하다. 연구소는 별도의 실증시설에 요구되는 고 비용을 줄이기 위해 현재 연구소가 보유하고 있는 조사재시험시설(IMEF)의 지하에 위치한 예비 hot cell을 활용키로 하고 차세대관리 종합공정의 특성 및 용도에 맞는 시설의 수정/보완 업무를 수행해오고 있다.(중략)

• Proceedings of the KSAR Conference
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• 2004.06a
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• pp.229-229
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• 2004

The identification of embryo-specific genes would provide insights into early embryonic development. However, the current methods employed to identify the genes that are expressed at a specific developmental stage are labor intensive and suffer from high rates of false positives. Here we employed a new and accurate reverse transcription-polymerase chain reaction (RT-PCR) technology that involves annealing control primers (ACPs) to identify the genes that are specifically or prominently expressed in bovine early blastocysts and hatched blastocysts produced in vitro. (omitted)

• Proceedings of the Korean Operations and Management Science Society Conference
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• 2004.05a
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• pp.409-412
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• 2004

Today, meta-heuristic algorithms have been much attention by researcher because they have the power of solving combinational optimization problems efficiently. As the result, many variants of a meta-heuristic algorithm (e.g., simulated annealing) have been proposed for specific application domains. However, there are few efforts to classify them into a unified software framework, which is believed to provide the users with the reusability of the software, thereby significantly reducing the development time of algorithms. In this paper, we present an object-oriented framework to be used as a general tool for efficiently developing variants of simulated annealing algorithm. The interface classes in the framework achieve the modulization of the algorithm, and the users are allowed to specialize some of the classes appropriate for solving their problems. The core of the framework is Algorithm Configuration Pattern (ACP) which facilitates creating user-specific variants flexibly. Finally, we summarize our experiences and discuss future research topics.

• Toxicological Research
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• v.25 no.3
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• pp.119-124
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• 2009

Genes related to Mycoplasma hyopneumoniae-induced inflammation were identified using the genefishing technology, an improved method for identifying differentially expressed genes (DEGs) using an annealing control primer (ACP) system in RAW264.7 cells. After treatment with M. hyopneumoniae, 16 DEGs were expressed in RAW264.7 cells using a pre-screening system. Among these 16 DEGs, 11 DEGs (DEGs 1, 4, 5-10, 12-15) were selected and sequenced directly, revealing that DEG12 (Grap), DEG14 (Gadd45), and DEG15 (secreted phosphoprotein 1) were related to inflammatory cytokines. This is the first report that intact M. hyopneumoniae induces the expression of Grap, Gadd 45${\beta}$, and secreted phosphoprotein 1 in RAW264.7 cells. Subsequently, these genes may be targets for screening novel inhibitors of the mycoplasmal inflammatory response.

• Journal of Microbiology and Biotechnology
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• v.19 no.5
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• pp.530-536
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• 2009

Cobalt chloride ($CoCl_2$) treatment of cells in vitro has been shown to induce cellular changes that are similar to those seen following hypoxia. To identify genes that are differentially expressed in response to treatment with $CoCl_2$, we compared the mRNA expression profiles of PC-3 cells that were treated with $CoCl_2$ with those of untreated PC-3 cells, using specific arbitrary primers and two anchored oligo(dT) primers provided in the ACP-based GeneFishing kits. The results of this study demonstrated that the puromycin-sensitive aminopeptidase (PSA) gene was down regulated in PC-3 cells that were treated with $CoCl_2$. This downregulation of PSA expression, in turn, suppressed the proliferation, migration, and invasion of PC-3 cells, as well as the secretion and expression of matrix metalloproteinase-9 (MMP-9).

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.12 no.5
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• pp.802-809
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• 2001

A linear power amplifier for the VHF frequency-hopping(FH) transmitter using an adaptive predistortion linearizer is designed. An analog polynomial linearizer as predistorter is employed. The recursive least square(RLS) algorithm is employed in the optimization process to minimize the errors between the predistorter and postdistorter output signals. Experimental results show that the adjacent channel power of the designed power amplifier is reduced by of 10 dB.

• Restorative Dentistry and Endodontics
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• v.37 no.4
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• pp.188-193
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• 2012

Mineral trioxide aggregate (MTA) was developed in early 1990s and has been successfully used for root perforation repair, root end filling, and one-visit apexification. MTA is composed mainly of tricalcium silicate and dicalcium silicate. When MTA is hydrated, calcium silicate hydrate (CSH) and calcium hydroxide is formed. Formed calcium hydroxide interacts with the phosphate ion in body fluid and form amorphous calcium phosphate (ACP) which finally transforms into calcium deficient hydroxyapatite (CDHA). These mineral precipitate were reported to form the MTA-dentin interfacial layer which enhances the sealing ability of MTA. Clinically, the use of zinc oxide euginol (ZOE) based materials may retard the setting of MTA. Also, the use of acids or contact with excessive blood should be avoided before complete set of MTA, because these conditions could adversely affect the hydration reaction of MTA. Further studies on the chemical nature of MTA hydration reaction are needed.

• Proceedings of the Korean Information Science Society Conference
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• 2000.04a
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• pp.451-453
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• 2000

메시지 순서도(Message Sequence Chart,MSC)는 ITU-T에서 국제적인 표준으로 제안되어 주로 전기 통신 교환 시스템과 같은 실시간 시스템을 위한 통신 행위에 대한 개괄적인 표현 방법으로서 널리 사용되어지고 있으며 요구 명세, 인터페이스 명세, 시뮬레이션 및 검정을 위해 사용되어지고 있다. MSC의 장점이라면 표현된 시스템의 행위를 직관적으로 이해할 수 있게 해주는 그래픽 표현을 제공하는 것이다. 의미론 입장에서 보면 MSC는 폴세스 대수 ACP의 변형인 PA$\varepsilon$에 의해 의미를 부여받고 있긴 하지만 이해하기가 난해한 것이 사실이다. 본 논문에서는 MSC의 동작적 의미를 분석하며 ACSR로 변환하여 그 의미를 보다 쉽게 파악하는 방법론에 대해 다룬다.

• The Korean Journal of Malacology
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• v.12 no.1
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• pp.19-31
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• 1996

국내 11개 지역(경기도 옹진군 덕적도, 경기도 의정부시 소요산, 강원도 춘천시, 충청남도 태안군 안홍, 경상북도 울릉군 울릉도, 전라북도 부안군 변산, 전라남도 신안군 홍도, 흑산도 비금도, 진도, 제주도)에서 채집된 달팽이 (A. despecta sieboleiana)를 대상으로 외부형태 분석과 동위효소를 검출하여 각 지역 집단간의 형태적, 유전적 유연관계와 변이 정도를 연구한 결과는 다음과 같다. 외부형태에 의한 집단간 유연관계는 안홍과 덕적도집단(average taxonomic distance, D=0.358)이 형태적으로 가장 유사한 집단으로 나타났다. Polyacrylamide gel을 사용하여 11가지의 동위효소를 검출한 결과 AKP, ACP, AO, EST, GPD, HBDH, LDH, SDH, XDH의 동위효소에서 유전적 다형현상이 나타났다. 전 집단의 평균 다형형의 빈도는 59.19%이며 이형접합자 빈도는 0.263이고 각 집단의 유전적 차이치는 춘천과 울릉도 집단이 0.066(genetic distance)으로 가장 높은 유사성을 보였다. 지리적 분포에 따른 외부형태 변이와 유전적 변이와의 관계는 유의성이 없었다.

• Korean Journal of Environmental Biology
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• v.27 no.1
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• pp.95-99
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• 2009

Transcriptomic changes in the brain of Limanda yokohamae were investigated to understand the environmental condition of Masan Bay, Korea. Differentially expressed genes (DEGs) in the brain of the flat fish from Masan Bay were identified by comparing those from the reference site Gangneung using annealing control primers-based polymerase chain reaction. The results demonstrated that two different kinds of the cytoplasmic ribosomal proteins, 40 s ribosomal protein S27a and ribosomal protein L6, were identified by the BLAST searching followed by sequence analysis. These findings suggest that environmental status of Masan Bay could hinder protein synthesis that is required for maintaining brain functions and thus cause the dysfunction of fish physiology.