• Title/Summary/Keyword: acidic condition

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Purification and Characteristics of Protease Produced by Syncephalastrum racemosum PDA 132-2 from Korean Traditional Meju (메주 유래의 Syncephalastrum racemosum PDA 132-2가 생산하는 Protease의 정제 및 특성)

  • 유진영;임성일
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.28 no.5
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    • pp.1010-1016
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    • 1999
  • Protease related mold was isolated and selected as a starter culture for commercial production of meju. Isolated microorganism was identified as Syncephalastrum racemosum PDA 132 2. To obtain basic data about protease for production of soybean peptides and application of the strain in meju fermentation, we extrated and purified protease and charateristics of the enzyme were investigated. The optimum condition for the production of enzyme was pH 4.0, 30oC, 5 days. The protease was purified 19.7 folds by gel filtration and ion exchange chromatography and specific activity was 12.4unit/mg. The purified enzyme was 34kDa in size, thiol protease(100% inhibited by PCMB), and was acidic protease(stable between pH 2.0~5.0). Vmax of the enzyme was 2.14 g/min which was lower(1/50) than that of by Asp. wentti and B. subtilis.

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Dyeing Properties of Morus alba L. Fruit Powder on the Silk Fabric (오디 분말을 이용한 견직물의 염색성)

  • Bai, Sang-Kyoung
    • Fashion & Textile Research Journal
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    • v.10 no.5
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    • pp.779-783
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    • 2008
  • The main colorant of Morus alba L. fruit is anthocyanin which is a kind of flavonoid group. In this article, the dyeing properties of colorant extracted from Morus alba L. fruit were investigated on some silk fabrics. The results were as follows: The maximum absorbance of Morus alba L. fruit showed at 284 nm, and 516 nm by UV-VIS spectrophotometer. The dye adsorption increased according as dye concentration. The optimal dyeing temperature was $60^{\circ}C$ and the dye equilibrium was reached 40 minutes after dyeing. The highest K/S value of the colorant obtained at bath ratio 40:1. The effect of pH of dye solution showed higher K/S value at acidic than alkaline condition. Almost Mordants except pre-Fe and Sn did not significantly increased dye adsorption. The pre mordanted silk fabrics showed RP, P, and post mordanted ones showed PB, GY. The color fastnesses to light, washing, and perspiration were modified at pre-Sn mordanted samples.

Effect of Cell Source and pH of Culture Medium on the Production of Canthin-6-one Alkaloids from the Cell Cultures of Tongkat Ali (Eurycoma longifolia Jack)

  • Mahmud, Luthfi-Aziz;Chan;Boey
    • Journal of Plant Biotechnology
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    • v.6 no.2
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    • pp.125-130
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    • 2004
  • Callus and cell suspension cultures of Eurycoma longifolia Jack could be an alternative supply of 9-hydroxycanthin-6-one and 9-methoxycanthin-6-one. The callus tissues were initiated from leaves of different trees. The friable calli were used for the preparation of the cell suspension cultures of E. longifolia. The leaf explant of tree Eu-9 produced the most callus and also induced high cell biomass in the cell suspension culture, but it produced low quantity of 9-methoxycanthin- 6-one and 9-hydroxycanthin-6-one. The leaf explant from tree Eu-8 produced low quantity of callus and cell biomass, but produced the highest quantity of 9-methoxycanthin- 6-one and 9-hydroxycanthin-6-one. Optimum production of cell biomass was obtained on cell culture medium with pH 5.75 prior to autoclaving, but high alkaloids content could be induced in culture medium in acidic condition with pH 4.75 and 5.25 prior to autoclaving.

Electrorheological characteristics of poly(o-ethoxy)aniline nanocomposite

  • Sung Jun Hee;Choi Hyoung Jin
    • Korea-Australia Rheology Journal
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    • v.16 no.4
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    • pp.193-199
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    • 2004
  • Poly(o-ethoxy)aniline (PEOA)/organoclay nanocomposite was prepared via a solvent intercalation using chloroform as a cosolvent with organically modified montmorillonite (OMMT) clay. The PEOA initially synthesized from a chemical oxidation polymerization in an acidic condition at pH = 1 was intercalated into interlayers of the clay with $25\;wt{\%}$ clay content. Electrical conductivity of the PEOA/OMMT nano­composite was found to be controlled via the intercalating process. The synthesized PEOA/OMMT nano­composite was characterized via an XRD and a TGA, and then adopted as an electrorheological (ER) material. The PEOA/OMMT synthesized with controllable electrical conductivity without a dedoping pro­cess was found to show typical ER characteristics possessing a yield stress from both steady state and dynamic measurements under an applied electric field.

The One Bath One Step Dyeing of Cationized Nylon/Viscose Rayon Mixture Fabrics With Acid Dyes and Reactive Dyes (산성염료와 반응성염료에 의한 카티온화 나일론/비스코스레이온 교직물의 1욕1단 염색)

  • Sung, Woo-Kyung
    • Fashion & Textile Research Journal
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    • v.7 no.3
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    • pp.339-345
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    • 2005
  • It is difficult to dye nylon/viscose rayon mixture fabrics by one-bath one-step dyeing method, because acid dyes and reactive dyes require acidic dyebath for adsorption and alkaline dyebath for fixation respectively. In order to overcome the disadvantage of the conventional two bath two step dyeing method of nylon/viscose rayon mixture fabric, it was pretreated with cationizing agent containing chlorohydrine group in aqueous solution of sodium hydroxide. The pretreated nylon/viscose rayon mixture fabrics produced cationized fabrics that could be dyed with acid dyes and reactive dyes under neutral condition. This study was carried out to investigate dyeing possibilities, surface reflectance spectra and color characteristics of cationized nylon/viscose rayon mixture fabrics with acid dyes and reactive dyes in a non-electrolytic and neutral dyebath by one bath one step dyeing method.

The Study on the Mordanting and Dyeing Properties of Sophora Japonica L. (괴화의 염색성에 관한 연구)

  • 주영주;소황옥
    • Journal of the Korean Society of Costume
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    • v.52 no.3
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    • pp.19-27
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    • 2002
  • This paper surveys the mordanting and dyeing properties of Sophora japonica L. Appropriate extraction, dyeing and mordanting condition of Sophora japonica L. were determined, and the effect of mordanting method on dye uptake and color fastness of dyed fabric was investigated. The maximum absorbance of Sophora japonica L. solution was 367.6mn, rutin solution was 365.6mn. The color of Sophora japonica L. solution was affected at pH 2 and pH 8~9. The optimum temperature to extract Sophora japonica L. was during 1 hour in 8$0^{\circ}C$. The effective dyeing temperature and time of silk were 10$0^{\circ}C$, 60min. K/S value of dyeing fabrics was increased by pre-mordanting treatment, especially Fe, Sn, Cr. In the case of Sophora japonica L. light fastness was increased by Fe mordanting. Perspiration fastness was better in acidic solution than that in alkaline solution. Fastness to rubbing and dry-cleaning were good in general.

Natural dyeing with walnut hull(Ⅰ) - Dyeing properties of wool fabric - (호도외피를 이용한 천연염색(Ⅰ) - 양모의 염색성 -)

  • Sin, Yun Suk;Mun, Seong In
    • Textile Coloration and Finishing
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    • v.14 no.2
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    • pp.102-102
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    • 2002
  • The purpose of this study is to investigate dyeing properties of wool fabric with walnut colorants. Walnut colorants were extracted with water from walnut hull and characterized by FT-IR analysis. Effects of dyeing conditions and mordanting on dyeing properties and colorfastness were studied. Results obtained were as follows, 1. Higher K/S value was obtained at higher temperature and longer dyeing time, the highest K/S value was shown at 100℃, 60min. 2. Dye uptake was increased with the increase in colorants concentration up to 2%. Higher K/S value was obtained at acidic condition. 3. Mordanting was not effective for improving dye uptake and colorfastness and for changing color of the dyed fabric.

Natural dyeing with walnut hull( I ) - Dyeing properties of wool fabric - (호도외피를 이용한 천연염색( I ) - 양모의 염색성 -)

  • 신윤숙;문성인
    • Textile Coloration and Finishing
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    • v.14 no.2
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    • pp.26-32
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    • 2002
  • The purpose of this study is to investigate dyeing properties of wool fabric with walnut colorants. Walnut colorants were extracted with water from walnut hull and characterized by FT-IR analysis. Effects of dyeing conditions and mordanting on dyeing properties and colorfastness were studied. Results obtained were as follows, 1. Higher K/S value was obtained at higher temperature and longer dyeing time, the highest K/S value was shown at $100^\circ{C}$, 60min. 2. Dye uptake was increased with the increase in colorants concentration up to 2%. Higher K/S value was obtained at acidic condition. 3. Mordanting was not effective for improving dye uptake and colorfastness and for changing color of the dyed fabric.

Streptozotocin의 췌장독성을 유발하는 활성 본체

  • 정진호;김부영;김미정;이주영
    • Proceedings of the Korean Society of Applied Pharmacology
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    • 1993.04a
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    • pp.175-175
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    • 1993
  • Streptozotocin, which is a naturally occurring nitrosoamide used extensively to produce diabetes mellitus in experimental animals, has been known to be chemically stable only under acidic condition (pH 4). The present study was conducted to determine whether its degradation products formed under various conditions can induce hyperglycemia in female rats. Streptozotocin in phosphate buffer saline (pH 7.4) resulted in spontaneous degradation rapidly. Furthermore, streptozotocin incubated with plasma isolated from rats was degraded even faster than those in neutral buffer. When streptozotocin was administered iv to rats, significant elevations in blood glucose level were observed within 24 hours. However, rats treated with equimolar concentration of streptozotocin degradation products under the phosphate buffer saline as well as the plasma did not lead to hyperglycemia. These results suggest that, when streptozotocin administered undergo spontaneous breakdown in vivo, its degradation Products do not induce the hyperglycemia in rats.

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The Inhibitory effect of omeprazole-cholestyramine resin in gastric secretion of rat (Omeprazole-cholestyramine resin 제제의 위산분비에 대한 억제효과)

  • 이영욱;김일웅;정지훈;라현오;최경범;이남인;손의동;허인회
    • Biomolecules & Therapeutics
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    • v.8 no.4
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    • pp.318-324
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    • 2000
  • We have examined inhibitory erects on gasritis using omeprazole-cholestyramine resinate, which has been developed to increase the stability of omeprazole, the well-known proton pump inhibitor, in an acidic condition. To test the pharmacological action of this, we investigated the effect of omeprazole-cholestyramine resinate on indomethacin-induced gastritis in rats. Omeprazole was used as a reference drug. Orally administered omeprazole-cholestyramine resinate inhibited the indomethacin-induced gastritis in a dose-dependent manner. The inhibitory effect of omeprazole-cholestyramine resinate on the gastritis was similar to that of reference drug. In addition, rectal adminstration of the omeprazole-cholestyramine resinate inhibited the indomethacin-induced gastritis in a dose-dependent manner. The inhibitory effect of omeprazole-cholestyramine resinate was equipotent to reference drug. The basal gastric acid secretion was decreased when it was administered either orally or rectally. This inhibition of omfprazole-cholestyramine resinate was similar to that of omeprazole. These data suggest that omeprazole-cholestyramine resinate inhibit the gastritis in rats, and are comparable to omeprazole available in market.

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