• 제목/요약/키워드: acid-resistance

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Lactobacillus casei YIT 9018의 Shuttle Vector 개발을 위한 분자유전학적 연구

  • 유민;남진식;권오식;백영진
    • 한국미생물·생명공학회지
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    • 제25권5호
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    • pp.464-467
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    • 1997
  • A shuttle vector, pSHvec, was constructed for Lactobacillus casei (L. casei) YIT 9018 and JM1O9 by recombinant DNA technology. This vector contained the $\beta$-lactamase II gene from Bacillus cereus as a selection marker and replication origins for both Gram(+) and Gram(-) strains. It could transform the wild type L. casei YIT 9018 as well as E. coli JM109 and transformed cells were selected based on antibiotics resistance. The ability of L. casei YIT 9018 for curd formation in 11% skim milk was maintained even after transformation with pSHvec. The vector was stable as long as antibiotics were added to the medium. These results could contribute to the study of lactic acid bacteria for the industrial purpose at a genetic level.

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Identification of a Prophage-encoded Abortive Infection System in Levilactobacillus brevis

  • Feyereisen, Marine;Mahony, Jennifer;O'Sullivan, Tadhg;Boer, Viktor;van Sinderen, Douwe
    • 한국미생물·생명공학회지
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    • 제48권3호
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    • pp.322-327
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    • 2020
  • Abortive infection systems (Abi) are phage resistance systems that can be prophage-encoded. Here, two genes encoding an Abi system were identified on a prophage sequence contained by the chromosome of the Levilactobacillus brevis strain UCCLBBS124. This Abi system is similar to the two-component AbiL system encoded by Lactococcus lactis biovar. diacetylactis LD10-1. The UCCLBBS124 prophage-derived Abi system (designated here as AbiL124) was shown to exhibit specific activity against phages infecting L. brevis and L. lactis strains. Expression of the AbiL124 system was shown to cause reduction in the efficiency of plaquing and cell lysis delay for phages of both species.

The Growth Characteristics of ${\beta}\;-FeSi_2$ as IR-sensor Device for Detecting Pollution Material : The Usage of the Ferrocene-Plasma

  • Kim, Kyung-Soo;Jung, II-Hyun
    • 환경위생공학
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    • 제15권2호
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    • pp.102-111
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    • 2000
  • As IR-sensor for detecting pollution material, the iron silicide has a fit band gap, high physicochemical stability at high temperature and good acid resistance. The growing film was formed with the Fe-Si bond and the organic compound because plasma resolved the injected precursors into various active species. In the Raman scattering spectrum, the Fe-Si vibration mode showed at 250 {TEX}$cm^{-1}${/TEX}. The FT-IR peak indicated that the various organic compounds were deposited on the films. The iron silicide was epitaxially grown to β-phase by the high energy of plasma. The lattice structure of films had [220]/[202] and [115]. The thickness of the films increased with the flow rate of silane. But rf-power increased with decreasing the thickness. The optical gap energy and the band gap were shown about 3.8 eV and 1.182∼1.194 eV. The band gap linearly increased and the formula was below: {TEX}$E_g^{dir}${/TEX}= 8.611×{TEX}$10^{-3}N_{D}${/TEX}+1.1775

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단층/다층 LB 분자막의 내마멸 성능 평가 (Evaluation of Antiwear Performance of Mono/multilayer LB Molecular Films)

  • 안효석;김두인
    • Tribology and Lubricants
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    • 제19권6호
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    • pp.329-334
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    • 2003
  • The antiwear properties of amphiphilic monolayers and composite multilayer on silicon surface were investigated using micro­tribometer and Auger Electron Spectroscopy. Langmuir­Blodgett (LB) monolayers from behenic acid, 2,4­heneicosanedione(HD) and its lopper complex((HD)$_2$Cu) were fabricated on silicon surface and the composite multilayer of 5­bilayer of (HD)$_2$Cu was fabricated on the surface of octadecyltrichlorosilane (OTS) self­assembled monolayer (SAM). We observed that LB monolayers and the composite multilayer exhibited a steady and excellent friction response when compared with the OTS SAM. These LB mono and multilayer also showed much higher wear­resistance than the OTS SAM.

콘크리트 보강용 FRP 리바의 개발 및 내구 특성 (Development and Durability Characteristics of FRP Reinforcing Bar for Concrete Structures)

  • 원종필;박찬기;윤종환;황금식;조용진
    • 한국농공학회:학술대회논문집
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    • 한국농공학회 2003년도 학술발표논문집
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    • pp.371-374
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    • 2003
  • The corrosion of steel reinforcing bar(re-bar) has been the major cause of the reinforced concrete deterioration. FRP(Fiber-reinforced polymer) reinforcing bar has emerged as one of the most promising and affordable solutions to the corrosion problems of steel reinforcement in structural concrete. In this study, long-term durability performance of FRP re-bar were evaluated. The mechanical and durability properties of two type of CFRP- and GFRP re-bar were investigated; the FRP re-bars were subjected to alkaline solution, acid solution, salt solution and deionized water. The mechanical and durability properties were investigated by performing tensile and short beam tests. Experimental results confirmed the desirable resistance of FRP re-bar to aggressive chemical environment.

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우리나라 수도근권에서 분리된 Azospirillum spp.의 특성 (Characterization of Azospirillum spp. Isolated from Korean Paddy Roots)

  • 조무제;강규영;강성모;윤한대
    • 미생물학회지
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    • 제25권2호
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    • pp.129-136
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    • 1987
  • 영남작물시험장에서 질소비료 공급없이 재배합 40종의 수도품종으로부터 출수기전의 수도근권의 질소고정력을 in situacetylene 환원력 측정방법으로 시험한 결과 Azospirillum 균의 무질소 malate 배지에서 얻은 균주와 상관관계를 보였다. 이 중 질소고정력이 높은 6종의 Azospirillum을 분리 동정한 결고 5종은 A. lipoferum, 1종은 A. brasilense임을 확인한 후 이들 6종의 Azospirillum들의 당이용성, biotin 요구성, 항생제저항성, auxin 생산성, plasmid profile 및 균체단백질 pattern을 비교 분석하였다.

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The Effect of Solution Treatment on Intergranular Corrosion Resistance of a New Type Ultra Low Carbon Stainless Steel

  • Julin, Wang;Nannan, Ni;Qingling, Yan;Lingli, Liu
    • Corrosion Science and Technology
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    • 제6권3호
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    • pp.140-146
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    • 2007
  • In the paper, with corrosion velocity measurement and metallographic observation on specimens after sulfuric acid/ferric sulfate boiling experiment, intergranular corrosion tendency of the new type ultra low carbon stainless steel developed by ourselves which experienced solution treatment at different temperatures was evaluated. A VHX 500 super depth field tridimensional microscope was used to observe corrosion patterns on the sample surfaces. The depth and width of grain boundary corrosion groove were measured by the tridimensional microscope, which indicated that the corrosion degrees of the samples which received solution treatment at different temperatures are quite different. Transgranular corrosion at different degree occurred along with forged glide lines. After comparison it was proved that the stainless steel treated at $1100^{\circ}C$ performs very well against intergranular corrosion.

Synthesis, Characterization and Curing Studies of Thermosetting Epoxy Resin with Amines

  • Lakshmi, B.;Shivananda, K.N.;Mahendra, K.N.
    • Bulletin of the Korean Chemical Society
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    • 제31권8호
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    • pp.2272-2278
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    • 2010
  • A new hybrid thermosetting maleimido epoxy compound 4-(N-maleimidophenyl) glycidylether (N-MPGE) is prepared by reacting N-(4-hydroxyphenyl) maleimide (HPM) with Epichlorohydrin by using benzyltrimethylammonium chloride as a catalyst. The resulting compound possesses both the oxirane ring and maleimide group. The curing reaction of these maleimidophenyl glycidylether epoxy compound (N-MPGE) with amines as curing agents such as ethylendiamine (EDA), diethylentriamine (DETA) and triethylenetetramine (TETA), aminoethylpiperazine (AEP) and isophoronediamine, IPDA), are studied. Incorporation of maleimide groups in the epichlorohydrin provides cyclic imide structure and high cross-linking density to the cured resins. The cured samples exhibited good thermal stability, excellent chemical (acid/alkali/solvent) and water absorption resistance. Morphological studies by the SEM technique further confirmed the phase homogeneity net work of the cured systems.

항균제를 도포한 하수시설 콘크리트의 생화학적 부식특성 평가를 위한 폭로시험 (An Exposure Experiment for the Evaluation of Corrosion Properties of Sewage Concrete Covered with Antibiotics)

  • 노경민;이의배;김영덕;이병기;김규용;김무한
    • 한국콘크리트학회:학술대회논문집
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    • 한국콘크리트학회 2006년도 춘계 학술발표회 논문집(II)
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    • pp.73-76
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    • 2006
  • Sewage facilities mainly consisted of concrete structures are being deteriorated seriously by biodeterioration originated from sulfur-oxidizing bacteria. To prevent biochemical corrosion of the sewage concrete, antibiotics which prevent growth of sulfur-oxidizing bacteria were developed. Recently, to evaluate biochemical corrosion properties of concrete, antimicrobial performance and resistance to sulfuric acid were investigated respectively. But, in this study, to evaluate it complexly, concrete specimen covered with antibiotics were exposed in the actual sewage environment and were investigated about corrosion properties after three months. As a result, weight change ratio, nature potential and sulfuric ratio of concrete covered with antibiotics were less than plain concrete.

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플라이애쉬 및 고로슬래그를 치환한 라텍스 개질 콘크리트의 중성화 특성 (The Carbonation Property of Latex Modified Concretes with Fly ash and Blast Furnace Slag)

  • 정원경;홍창우;김경진;심도식;윤경구
    • 한국콘크리트학회:학술대회논문집
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    • 한국콘크리트학회 2006년도 춘계 학술발표회 논문집(II)
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    • pp.569-572
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    • 2006
  • This study was performed to investigate the characteristics of carbonation for latex modified concrete with fly ash and blast furnace slag. The experimental variables consisted of ground granulated blast-furnace slag contents (0%, 30%, 50%), fly ash contents (0, 30%) latex contents (0, 5, 10, 15%). Two different methods of carbonation test such as $CO_2$ gas and 5% sulphuric acid digestion resistance test were varied in this study.

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