This experiment was conducted to develop fertilizer which promotes plant growth as well as suppressing pathogenic fungi. The fertilizer was made from the mixture of Ju-Back (Korean rice wine cake) and indigenous rhizosphere-bacterium. The main ingredients of Ju-Back were investigated as 6.04% total nitrogen, 42.59% total carbohydrate, 1.01% available phosphate, 73.42% organic matter, 7.72% potassium oxide, 1.35% calcium oxide, 0.53% magnesium oxide. The enzyme activities of Ju-Back were estimated to be 980 units/g for ${\alpha}-amylase$, 300 units/g for glucoamylase, and 1800 units/g for acid pretense. Indigenous rhizosphere bacteria which produced antifungal agent were isolated from soil, and was selected KMU-13 strain which can antagonize against various plant pathogenic fungi (Botrytis cinerea KACC 40573, Sclerotinia sclerotiorum KACC 41065, Fusairum oxysporum KACC 40052, Pythium aphanidermatum KACC 40156, Phytophthora capsici KACC 40476 and Glomerella cingulata KACC 40299). KMU-13 strain was identified as Bacillus subtilis KMU-13 by biochemical and 16s rDNA analysis. The organic fertilizer was made as prototype which was composed 20% Ju-Back, 70% carrier, 9.7% microorganism cultivated solution, 0.3% trace-element. We also investigated an application of fertilizer using Ju-Back for cultivating lettuce (Lactuca sativar) which were grown in three soil conditions that had chemical fertilizer, barnyard manure, lime power, urea, potassium chloride and superphosphate as a control, the whole quantity (80 kg/10a) of posted fertilizer with the control and the half quantity (40 kg/10a) with the control. The growth characteristics were examined and analysed with several weeks interval from 3 weeks to 8 weeks on head length (cm), head width (cm/head), number of leaf and fresh weight (g/plant). The results are summarized as follows. The head width and fresh weight of lettuce were the highest at posted fertilizer 1 (whole quantity) was applied chemical, organic matter (Ju-Back) and carrier. The head length was the highest at posted fertilizer 2 (whole quantity) was applied Ju-Back only.
Jo, Min-Ki;Chae, Gi-Tak;Koh, Dong-Chan;Yu, Yong-Jae;Choi, Byoung-Young
Journal of Soil and Groundwater Environment
/
v.14
no.3
/
pp.1-13
/
2009
Alkalinity and total carbon contents were measured by acid neutralizing titration (ANT), back titration (BT), gravitational weighing (GW), non-dispersive infrared-total carbon (NDIR-TC) methods for assessing precision and accuracy of alkalinity and total carbon concentration in $CO_2$-rich water. Artificial $CO_2$-rich water(ACW: pH 6.3, alkalinity 68.8 meq/L, $HCO_3^-$ 2,235 mg/L) was used for comparing the measurements. When alkalinity measured in 0 hr, percent errors of all measurement were 0~12% and coefficient of variation were less than 4%. As the result of post-hoc analysis after repeated measure analysis of variance (RM-AMOVA), the differences between the pair of methods were not significant (within confidence level of 95%), which indicates that the alkalinity measured by any method could be accurate and precise when it measured just in time of sampling. In addition, alkalinity measured by ANT and NDIR-TC were not change after 24 and 48 hours open to atmosphere, which can be explained by conservative nature of alkalinity although $CO_2$ degas from ACW. On the other hand, alkalinity measured by BT and GW increased after 24 and 48 hours open to atmosphere, which was caused by relatively high concentration of measured total carbon and increasing pH. The comparison between geochemical modeling of $CO_2$ degassing and observed data showed that pH of observed ACW was higher than calculated pH. This can be happen when degassed $CO_2$ does not come out from the solution and/or exist in solution as $CO_{2(g)}$ bubble. In that case, $CO_{2(g)}$ bubble doesn't affect the pH and alkalinity. Thus alkalinity measured by ANT and NDIR-TC could not detect the $CO_2$ bubble although measured alkalinity was similar to the calculated alkalinity. Moreover, total carbon measured by ANT and NDIR-TC could be underestimated. Consequently, it is necessary to compare the alkalinity and total carbon data from various kind of methods and interpret very carefully. This study provide technical information of measurement of dissolve $CO_2$ from $CO_2$-rich water which could be natural analogue of geologic sequestration of $CO_2$.
The issue of acid precipitation and related environmental problems in East Asia has been emerging. To evaluate the acidity and chemical characteristics of rainwater in Korea, its chemical properties during cultivation season from April to October were investigated at Taean in 2007. Also, to estimate the contribution of ions on its acidity, ion composition characteristics and neutralization effects by cation ions were determined. The ion balance between cations and anions values showed high correlation. The mean values of pH and EC were 4.9 and $32.9{\mu}S\;cm^{-1}$, respectively. The EC of rainwater showed seasonal characteristic, which was $91.4{\mu}S\;cm^{-1}$ with relatively low rainfall compared with other monitoring periods. $Na^+$ was the main cation followed by $NH_4{^+}$ > $Ca^{2+}$ > $H^{+}$ > $Mg^{2+}$ > $K^+$. Among these ions, $Na^{+}$ and $NH_4{^+}$ covered over 70% of total cations. In the case of anion, the order was $SO_4{^{2-}}$ > $NO_3{^-}$ > $Cl^{-}$. The mean value of sulfate, which is main anion component in the samples was $152.1{\mu}eq\;L^{-1}$. Also, 90% of soluble sulfate in rainwater was $nss-SO_4{^{2-}}$(non-sea salt sulfate). With fractional acidity and theoretical acidity of rainwater samples, $NH_4{^+}$ and $Ca^{2+}$ contributed greatly in neutralizing the rain acidity.
The rear edge population is considered to have low genetic diversity and high risk of extinction according to a highly isolated distribution. However, the rear edge population is observed to have persisted for an extended period despite the low genetic diversity. As such, it is necessary to understand the ecological process involved in the persistence of the population. Viola mirabilis L. in Korea is considered the rear edge population from the perspective of the worldwide distribution. We surveyed the distribution range of V. mirabilis, which shows the isolated distribution in the central area of Korea, to find out the factors of its persistence. Next, we investigated and accessed the vegetational pattern of habitats, soil environment, phenology, self-compatibility, population structure, and extinction risk factors observed in the distribution area. V. mirabilis was distributed in the understory of the deciduous forest, planted forest of the deciduous conifer and deciduous broad-leaved trees, shrubland, and grassland in the limestone area. We also observed the re-establishment of seedlings in the population, and most of them showed a stable population structure. For chasmogamous flowers, the visit by pollinators has a significantly positive relationship with the production of fruits. However, we found that the production of the cleistogamous flowers was more numerous in all studied populations and that only the cleistogamous flowers were produced despite a more substantial plant size in some populations. The plant size was more related to the production of the cleistogamous flowers than that of the chasmogamous flowers. Accordingly, the cleistogamous flowers significantly contributed to seedling recruitment in the population. We found that the production of the chasmogamous flowers and the cleistogamous flowers did not have a correlation with the factors of the soil analysis except for phosphoric acid. V. mirabilis showed the self-incompatibility characteristics most likely due to the production capability of the cleistogamous flowers. Potential extinction risk factors observed in the distribution area was included the development of limestone mine, the expansion of agricultural fields, and the construction of houses. Although V. mirabilis showed an isolated distribution in the limestone area in the Korean peninsula, it showed a diverse distribution in a wide habitat environment ranging from the grassland to the understory of the trees with relatively low canopy closure rate. Moreover, we concluded that the persistence of the population was possible if we can maintain the current state of multiple populations and stable population structure.
Journal of the Korean Institute of Landscape Architecture
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v.50
no.2
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pp.41-63
/
2022
The purpose of this study is to secure objective and precise data through ecosystem monitoring, to reveal ecological characteristics through comparison and analysis with past survey data, and to accumulate basic data for diagnosing the current situation and predicting changes in the ecosystem. The target site is the 'Quercus mongolica forest on the Buksa-myeon of Namsan', which was designated as an Ecological Landscape Conservation Area (ELCA) of Seoul in July 2006. The research contents are analysis of soil environment change (1986~2016), change of actual vegetation (1978~2016), and change of plant community structure (1994~2016). A total of 8 fixed surveys (400~1,200m2) were established in 1994 and 2000. Analysis items are importance value, species and population, and Shannon's species diversity. The soil environment of Namsan is acidic (pH 4.40 in 2016), which is expected to have a negative impact on tree growth and vegetation structure due to its low capacity for exchangeable cations. Quercus mongolica forest in Namsan is mainly distributed on the northern slopes. The actual vegetation area changed from 49.4% in 1978 → 80.7% in 1986 → 82.4% in 2000 → 88.3% in 2005 → 88.3% in 2009 → 70.3% in 2016. In 2016, the forest decreased by 18% compared to 2009. While there was increased growth of Quercus mongolica in the tree layer from 2009 to 2016, the overall decline in vegetation area was due to logging and fumigation management following the spread of oak wilt in 2012. As for the changes in the plant community structure, Quercus mongolica of the tree layer was damaged by oak wilt, and the potential vegetation that can form the next generation was ambiguous. In the subtree layer, the force of urbanization tree species such as Styrax japonicus, Sorbus alnifolia, and Acer palmatum. was maintained or increased. In the shrub layer, the number of trees and species increased significantly due to the open tree crown, and accordingly, the species diversity of Shannon for woody plants also increased. In Quercus mongolica forest of Namsan, various ecological changes are occurring due to the effects of urban environments such as air pollution and acid rain, the limitation of Quercus mongolica pure forest due to oak wilt, and the introduction of exotic species, thus, it is necessary to establish a management plan through continuous monitoring.
This study was conducted to examine the physiology of pine mushroom mycelia cultured with various media for artificial culture of pine mushroom. The results obtained were as follows: 1) Among the various media, the medium composed of honey, boiled pine mushroom and soil extract fluid, fibrous root extract fluid, dry yeast, $KH_2PO_4$ inositol, folic acid, and biotin was the best for the growth of pine mushroom mycelium. 2) The optimum temperature for germinating pine mushroom spore and for culturing pine mushroom mycelium, was $24^{\circ}C$ and the optimum pH was 4.5. 3) There was no significant difference in growth between the mycelium separated from the tissue of pine mushroom sporophore and that separated from the spore. 4) No noticeable effect was found on the growth if such salts as $ZnSO_4$, $MnSO_4$, $MgSO_4$, $CaCl_2$ and ferric citrate were added to the Hamada's medium. 5) The addition of fibrous root extract promoted the growth of pine mushroom mycelium. 6) As a carbon source of artificial media, honey was more effective than glucose. 7) The culture infiltration of Mortierlla growing often in Fairy Ring was good for the growth of mycelium compared with the control. 8) The addition of fibrous root extract, inositol, biotin, and folic acid to artificial culture media was greatly effective in growth. When the temperature was lowered $19^{\circ}C$ after mycelium has appeared, the formation of primordium was observed.
The field experiment was performed to investigate the effects of various kinds of phosphorus fertilizers such as double superphosphate, fused magnesium phosphate and Simagcarin (both the Kyun-gi Chemical Co, products) on the physiological roles in development of root system, growth and yield compositions of rice plant. Radioactive phosphoric acid $(H_3\;^{32}PO_4)$ was applied to measure the root activity. 1. The number of total tillers was significantly increased in double superphosphate plots, but the rate of fruitful tillers was more numerous in the fused magnesium phosphate and the Simagcarin plots than that of the other plots. 2. The grain yield was much more obtained in the fused magnesium phosphate and Simagcarin plots (no significant difference were found between both of plots) than the double superphosphate and control plots. It seemed due to the increasing of seedbearing rate and number of fruitful tillers. 3. In double superphosphate plots, root system was mostly developed near topsoil areas, but fused magnesium phosphate and the Simagcarin plots, root system was uniformly distributed from topsoil to subsoil areas. 4. As the results of those experiments, fused magnesium phosphate and Simagcarin was demonstrated to be soil amendmentical materials rather than the phosphorus fertilizers, especially in low productive paddy soils which lack the special mineral nutritions.
We investigated the effect of silicate coating of rice seeds on bakanae disease incidence and the quality of seedlings raised in seedling boxes and transplanted into pots. The silicate-coated rice seed (SCS) was prepared as follows. Naturally infested rice seeds not previously subjected to any fungicidal treatment were dressed with a mixture of 25% silicic acid at pH 11 and 300-mesh zeolite powder at a ratio of 50 g dry seed - 9 mL silicic acid - 25 g zeolite powder. The following nursery conditions were provided : Early sowing, dense seeding in a glass house with mulching overnight and no artificial heating, which were the ideal conditions for determining the effect on the seed. The nursery plants were evaluated for Gibberella. fujikuroi infection or to determine the recovery to normal growth of infected nursery plants in the Wagner pot. Seedlings emerged 2-3 days earlier for the SCS than they did for the non-SCS control, while damping-off and bakanae disease incidence were remarkably reduced. Specifically, bakanae disease incidence in the SCS was limited to only 7.8% for 80 days after sowing, as compared to 91.6% of the non-SCS control. For the 45-days-old SCS nursery seedlings, the fresh weight was increased by 11% and was two times heavier, with only mild damage compared to that observed for non-SCS. Even after transplanting, SCS treatment contributed to a lower incidence of further infections and possibly to recovery of the seedlings to normal growth as compared to that observed in symptomatic plants in the pot. The active pathogenic macro-conidia and micro-conidia were considerably lower in the soil, root, and seedling sheath base of the SCS. In particular, the underdeveloped macro-conidia with straight oblong shape without intact septum were isolated in the SCS ; this phenotype is likely to be at a comparative etiological disadvantage when compared to that of typical active macro-conidia, which are slightly sickle-shaped with 3-7 intact septa. A active intact conidia with high inoculum potential were rarely observed in the tissue of the seedlings treated only in the SCS. We propose that promising result was likely achieved via inhibition of the development of intact pathogenic conidia, in concert with the aerobic, acidic conditions induced by the physiochemical characteristics associated with the air porosity of zeolite, alkalinity of silicate and the seed husk as a carbon source. In addition, the resistance of the healthy plants to pathogenic conidia was also important factor.
Journal of Korean Society for Atmospheric Environment
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v.10
no.E
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pp.371-386
/
1994
Ecological examinations of the radial growth Patterns of pine trees(Pinus densiflora Sieb. et Zucc) growing on Mt. Namsan in central Seoul were made to test a Proposition that the pine trees decline due to the influence of air pollution and acid rain, which was proposed by some researchers in Korea, and the potential effects of current level of air pollutants to the growth of the Pine trees in central Seoul have been speculated. Tree-rings of 40 trees sampled at 3 sites of Mt. Namsan were prepared and examined using a Computer-aided Tree-Ring Measuring System at Kookmin University, Korea. Air Pollutant data collected by the Ministry of Environment( MOE ) and the Forestry Research Institute(FRI) were used to infer the general conditions of the environment. Correlation analysis was applied to the data set of tree growth and the other environmental factors. General information derived from the close examination of the tree-rings and the data on air pollution, drought and the other biological conditions suggested that the growth of the pine trees was severely affected by the occurrence of drought(climatic variation), the prevalence of the pine leaf gall midges(insects), and the suppression by the black locust trees(Robinia pseudo-acacia L.) (competition among trees). While the current condition of air pollution in Seoul cannot be categorized as good, the concentrations of air pollutants are not so high as to cause acute damages to the trees. In addition, while the data of rain acidity showed episodic low PHs of under 4.0, the average of them is far less acidic than those which were observed in either northeastern United States or central Europe, where the decline of trees were not solely attributed to any of the air pollutants. Considering the sequential facts that one of the most important environmental factors that affect the growth of trees is weather condition of the forest that the proposition of the decline of the pine trees was made without careful examination of the growth patterns and past growth history of them as well as the complex influences of many other factors including the weather conditions to the growth of trees, and that no objective explanation has been made on the causal relationships between the current condition of air pollution and the growth of the trees, such a proposition should be evaluated as invalid for the explanation of tree growth on Mt. Namsan in central Seoul, Korea. The author evaluates the factors of air pollution (including acid rain) as the predisposing factors, which may have the Potentials to chronically affect the tree growth at the forest ecosystem on Mt. Namsan for a long period of time. Ecosystem ecological studies should be further carried out to carefully explain both the functional and the structural aspects of the ecosystem processes, which include the biogeochemistry and the long-term changes of soil conditions as well as the growth of the other tree species on the mountain.
This study was carried out in order to develop a biological control of anthracnose of red pepper caused by fungal pathogens. In particular, this study focuses on the Colletotrichum species, which includes important fungal pathogens causing a great deal of damage to red pepper. Antagonistic bacteria were isolated from the soil of pepper fields, which were then tested for biocontrol activity against the Colletotrichum gloeosporioides anthracnose pathogen of pepper. Based on the 16S rRNA sequence analysis, the isolated bacterial strain CS-52 was identical to Bacillus sp. The culture broth of Bacillus sp. CS-52 had antifungal activity toward the hyphae and spores of C. gloeosporioides. Moreover, the substances with antifungal activity were optimized when Bacillus sp. CS-52 was grown aerobically in a medium composed of 0.5% glucose, 0.7% $K_2HPO_4$, 0.2% $KH_2PO_4$, 0.3% $NH_4NO_3$, 0.01% $MnSO_4{\cdot}7H_2O$, and 0.15% yeast extract at $30^{\circ}C$. The inhibition of spore formation resulting from cellulase, siderophores, and indole-3-acetic acid (IAA), were produced at 24 h, 48 h, and 72 h, respectively. Bacillus sp. CS-52 also exhibited its potent fungicidal activity against anthracnose in an in vivo test, at a level of 70% when compared to chemical fungicides. These results identified substances with antifungal activity produced by Bacillus sp. CS-52 for the biological control of major plant pathogens in red pepper. Further studies will investigate the synergistic effect promoting better growth and antifungal activity by the formulation of substances with antifungal activity.
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