• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2000년도 춘계학술발표대회
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• pp.231-234
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• 2000

본 연구에서는 담자균류의 일종인 Agaricus blazei를 이용한 polysaccharide의 생성을 증가시키고자 glutamic acid의 첨가시기의 영향을 비교${\cdot}$검토하였다. 그결과 2 g/L의 glutamic acid를 배양 4일째인 대수증식기에 첨가하여 polysaccharide 생성을 유도한 경우 배양 7일째에 12.9 g/L의 균체량과 9.1 g/L의 polysaccharide를 생성하였다. 그러나 이런한결과는 배양 초기에 glutamic acid를 첨가했을경우와 비교하여 볼 때 균체량은 다소 증가 했으나 polysaccharide 생성량에 있어서는 거의 유사한 결과를 나타내는 것이다. 생물반응기를 이용한 회분배양에 있어서도 2 g/L의 glutamic acid를 대수증식기인 배양 4일째에 첨가한 경우 균체량은 13.5 g/L, polysaccharide 생성량은 9.9 g/L를 생산하여 배양결과 최대 생산량을 나타내었다.

• Food Science and Biotechnology
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• 제18권3호
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• pp.749-754
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• 2009

The polysaccharide (BB-pol) extracted from Bifidobacterium bifidum BGN4 showed growth inhibitory effects on several colon cancer cell lines such as HT-29 and HCT-116. To increase the yield of polysaccharide, B. bifidum BGN4 was cultured in various culture media with different compositions. When B. bifidum BGN4 was cultured in modified MRS broth containing phytic acid, the cells showed increased branch formation and enlarged morphology. The content of total carbohydrate and the ability of adhesion to intestinal epithelial cells were also increased by phytic acid. The polysaccharide obtained from the cells grown in the presence of phytic acid inhibited the proliferation of cancer cell lines such as HT-29 and MCF-7 cells but not normal colon cell line, FHC. Taken together, Bifidobacterium grown in the presence of phytic acid may confer enhanced beneficial function for the host.

• 분석과학
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• 제30권4호
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• pp.205-212
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• 2017

Rice endosperm, the portion that remains after milling, is the part of the rice seed that is primarily consumed as a source of nutrients. There have been many studies on polysaccharides, such as hemicellulose, cellulose, and pectins, derived from the cell walls of various plant groups. It has been reported that the acidic polysaccharide fractions, which contain water-soluble pectins that have been shown to have pharmacological effects in vivo and in vitro, have common chemical structures that include galacturonic acid polymers, rhamnose, arabinose, and galactose. However, few studies have been conducted on the acidic polysaccharides contained in the endosperm of rice. In this study, we quantitatively compared the differences in the acidic polysaccharide contents from samples from two of the main varieties of rice consumed as staple foods, japonica and indica, using a colorimetric method. Rice samples were collected from 39 different regions in Korea, China, Thailand and Vietnam. Acidic polysaccharide fractions were obtained by precipitation of the alcohol-insoluble residue (AIR) and enzyme treatment of each sample. The total amount of carbohydrates and uronic acid in each acidic polysaccharide fraction were measured using the phenol-sulfuric acid method and the carbazole-sulfuric acid method, respectively. The differences in the total polysaccharide contents in the acidic polysaccharide fractions were not statistically significant (p = 0.07), but the uronic acid contents were significantly different between the two groups (p = 0.04).

• 생약학회지
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• 제42권1호
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• pp.82-88
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• 2011

This study was carried out to investigate the optimum conditions for extraction of soluble acidic polysaccharides from ginseng marc. Method of carbazole-sulfuric acid was applied to determine the amount of acidic polysaccharides in ginseng marc. The amounts of soluble acidic polysaccharides in water extract of ginseng marc were increased with increasing extraction temperature. The contents of acidic polysaccharides were not significantly different despite the extraction time increasing from 0.5 hours to 6 hours. To estimate the rehydration rate of the freeze dried polysaccharide, the extracted acidic polysaccharide fraction powder was determined the amount of soluble acidic polysaccharides by carbazole-sulfuric acid method again. The rehydration rate of acidic polysaccharides from water-extract of red ginseng marc at room temperature was 100%. On the other hand, the rehydration rate of acidic polysaccharide of red ginseng marc at boiling temperature was about 50%. The rehydration rate of acidic polysaccharides from water-extract of white ginseng marc at room temperature was 50%. The rehydration rate of acidic polysaccharide of red ginseng marc at boiling temperature was about 40%. The rate of soluble acidic polysaccharide of Red Ginseng is higher than that of White Ginseng. We can find out the maximum extraction method of soluble acidic polysaccharide from ginseng marc.

• Journal of Ginseng Research
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• 제17권2호
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• pp.139-144
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• 1993

The method for colorimetric determination of acidic polysaccharide from Panax ginseng was investigated. It is possible to apply the method of carbazole-sulfuric acid to determination of pectin, and also to measure the amount of pectin in the mixture of various high molemlu compounds such as starch. cellulose and gum, etc. When the method of carbazole-sulfuric acid was applied to determine the amount of acidic polysaccharide, optical density at 525 nm increased linearly with an increase in the concentration of pure acidic polysaccharide. Effective extraction temperature with water for the determination of the amount of ginseng acidic polysaccharide (GAP) was $80{\circ}C$. In order to separate or concentrate GAP it was appropriate to precipitate the extract only once with 80% ethyl alcohol. GAP was very stable at $100{\circ}C$ for 4 hrs in aqueous solution and between pH values of 5.0~ 12.0.

• 한국식품과학회지
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• 제23권3호
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• pp.291-295
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• 1991

초산균의 변이주 중 다당류 생산량이 우수한 균수를 선별한 결과 Acetobacter pasteurianus IFO 13751-5가 친주보다 약 3배의 다당류 생산량을 나타내었다. 돌연변이주인 Acetobacter pasteurianus IFO 13751-5의 최적 배양조건을 검토한 결과 탄소원은 sucrose로써 5% 첨가시 45.95 mg/ml로 가장 높게 나타났다. 다당류를 대량생산할 목적으로 jar fermentor를 이용하여 생산된 다당류는 72시간에서 55.10 mg/ml의 최대 생산량을 나타내었다. 이 배양액을 분리 정제하여 진공 동결건조한 다당류는 18.93 g/l을 얻었다.

• Journal of Microbiology and Biotechnology
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• 제14권6호
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• pp.1324-1326
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• 2004

In previous studies, we isolated an isogenic LPS mutant of Bradyrhizobium japonicum 61A101C, which was completely devoid of O-polysaccharide and had altered cell surface characteristics. Subsequently, the mutated gene was identified, cloned, and used to complement the LPS mutant strain JS314 to restore the phenotype. Since it has been reported that in Escherichia coli LPS O-polysaccharide is involved in resistance to an organic acid such as acetic acid under low pH (Barna et al., Molecular Microbiology 43: 629-640, 2002), we compared the organic acid resistance of the three B. japonicum strains; wild-type 61A101C, the LPS mutant JS314, and the complemented strain to determine whether the role of O-polysaccharide in the resistance to organic acid could be generalized. Growth of all three strains was inhibited by the presence of 3 mM acetic acid under acidic condition (pH 5.5). To our surprise, however, in the presence of 2 mM acetic acid, wild-type and the complemented strains did not grow while the $LPS^-$ mutant showed a significant growth. Therefore, unlike in E. coli, the lack of O­polysaccharide of LPS appears to render B. japonicum more resistant to organic acid.

• 통합자연과학논문집
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• 제8권2호
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• pp.111-116
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• 2015

Liquid chromatographic enantiomer separation of ${\alpha}$-amino acid esters as nitrobenzoxadiazole (NBD) derivatives was performed using several chiral stationary phases (CSPs) based on polysaccharide derivatives under fluorescence detection. For enantiomer separation by normal HPLC, the non-aqueous derivatization method of ${\alpha}$-amino acid esters for NBD analytes was introduced. Among the six CSPs used in this study, the performance of Chiralpak IA was superior for enantiomer resolution of NBD derivatives of several ${\alpha}$-amino acid methyl esters. Also the convenient analytical method using polysaccharide-derived CSPs developed in this study was applied to determine the optical purity of ${\alpha}$-amino acids esters. It was investigated that the enantiomeric impurity levels of 0.02-1.73% were found after determination of enantiomeric purities of several commercially available L-amino acid methyl esters. It is expected to be quite useful for enantiomer separation of other ${\alpha}$-amino acid esters as NBD derivatives by normal HPLC.

• KSBB Journal
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• 제16권4호
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• pp.370-375
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• 2001

생물고분자 생산 균주를 분리하여 동정한 결과, 분리균주는 Gram 음성의 간균으로 내생포자를 형성하지 않았으며, nitrate 환원과 catalase 반응에서 양성이고, oxidase 반응에서 음성인 통성 혐기성의 한 균주로 분류학상의 위치를 검토한 결과, Enterobacter sp.로 판단하였다 이 균주가 생성하는 생물고분자는 정색반응, CPC 침전, IR 분석 등에 의해 우론산을 함유한 산성의 다당인 것으로 추정되었다. TLC 및 GC에 의해 조사한 결과, mannose, galactose 및 galacturonic acid로 구성된 hetero 다당이었으며, 분자량은 약 3.7$\times$$10^{5}$이었다. 이다당의 수용액(0.5-2.0%, w/v)은 비뉴우톤 유체로 항복응력을 갖는 의가소성 유체로서 거동하였으며, 1.5% 농도 이상의 낮은 농도에서 겔 형성능을 나타내었다.다.

• Preventive Nutrition and Food Science
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• 제6권3호
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• pp.137-141
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• 2001

Water-soluble crude polysaccharide (TM-1) prepared from the leaves of Teucrium viscidum var. miquelianum was fractionated into three polysaccharide fractions, TM-2, TM-3 and TM-4 by the addition of cetyltrimethylammonium bromide. The major polysaccharide fraction, TM-2, consisted of glucose, galactose, rhamnose, mannose, glucuronic acid and galacturonic acid, and all fractions contained galactose and glutose as the major neutral sugars. TM-4 showed the highest anti-complementary activity. When TM-4 was futher fractionated by anion exchange chromatography, TM4- II a and TM4-II b showed the most Potent anti-complementary activity. TM4- II a was composed mainly of galactose, arabinose and glucose in the molar ratios of 2.13 : 0.94 : 1.00 respectively, and contained a small amount of galacturonic acid and glucuronic acid.