• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2000년도 춘계학술발표대회
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• pp.227-230
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• 2000

Batch cultivations of Anaerobiospirillum succiniciproducens have been systematically studied for the economical production of succinic acid from wood hydrolysate with corn steep liquor(CSL) as a nitrogen source. CSL was found to be an alternative complex nitrogen source for A. succiniciproducens when glucose and wood hydrolysate were used as carbon sources. Compared with polypeptone and/or yeast extract, CSL had similar effects on fermentation performance such as succinic acid yield and a ratio of succinic acid to acetic acid in the fermentation of wood hydrolysate as well as glucose. This means that succinic acid can be produced more economically from wood hydrolysate and CSL than relatively expensive carbon and nitrogen sources. Besides its low cost, the alternative medium served as a green technology for succinic acid production because it gives a net-zero effect on global warming.

• Journal of Ginseng Research
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• 제13권1호
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• pp.1-4
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• 1989

Linoleic acid and ferulic acid were isolated from alkaline hydrolysate of a crude ginseng saponin fraction of Panax ginseng C.A. Meyer roots.

• 한국식품영양학회지
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• 제7권2호
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• pp.151-158
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• 1994

This study was carried out to clear up the cause of low-acid producing phenomenon occurred In non-fat dry milk during liquid-yoghurt fermentation by Lactobacillus case, and to present its improving methods. All samples of non-fat dry milks which were low in TCA-soluble peptides showed low-acid production, but those high in TCA-soluble peptides showed high-acid production. The addition of trypsin-hydrolysate of Na-caseinate to non-fat dry milk showed some improving effect on acid production but that of papain-hydrolysate did not show any improving effect and that of bacterical neutral protease-hydrolysate showed some inhibitory effect. The improving effects on growth and acid production of lactic acid bacteria were more prominent when the trypsin-hydrolysate of Na-caseinate was added. to such fermenting system in which the levels of TCA-soluble peptides and the proteolytic ability of starter bacteria were abnormally low. The liquid-yoghurt made with non-hydrolysed Na-caseinate and defective non-fat dry milk showed precipitate occurrence but that with trypsin-hydrolysate of Na-caseinate and defective non-fat dry milk did not make any precipitate during storage as with normal non-fat dry milk.

• 한국해양바이오학회지
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• 제14권1호
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• pp.9-24
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• 2022

Low-molecular weight peptides derived from fish collagen exhibit several bioactivities, including antioxidant, antiwrinkle, antimicrobial, antidiabetic, and antihypertension effects. These peptides are also involved in triglyceride suppression and memory improvement. This study aimed to investigate the optimal processing condition for preparing low-molecular weight peptides from flounder skin, and the properties of the hydrolysate. The optimal processing conditions for peptic hydrolysis were as follows: a ratio of pepsin to dried skin powder of 2% (w/w), pH of 2.0, and a temperature of 50℃. Peptic hydrolysate contains several low-molecular weight peptides below 300 Da. Gly-Pro-Hyp(GPHyp) peptide, a process control index, was detected only in peptic hydrolysate on matrix-assisted laser desorption/ionization-time-of-flight(MALDI-TOF) spectrum. 2,2'-azinobis-(3-3-ethylbenzothiazolline-6- sulfonic acid(ABTS) radical scavenging activity of the peptic hydrolysate was comparable to that of 1 mM ascorbic acid, which was used as a positive control at pH 5.5, whereas collagenase inhibition was five times higher with the peptic hydrolysate than with 1 mM ascorbic acid at pH 7.5. However, the tyrosinase inhibition ability of the peptic hydrolysate was lower than that of arbutin, which was used as a positive control. The antibacterial effect of the peptic hydrolysate against Propionibacterium acne was not observed. These results suggest that the peptic hydrolysate derived from a flounder skin is a promising antiwrinkle agent that can be used in various food and cosmetic products to prevent wrinkles caused by ultraviolet radiations.

• Korean Chemical Engineering Research
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• 제52권6호
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• pp.789-795
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• 2014

초산은 묽은 산에 의해 처리되어 얻어진 헤미셀룰로오스 가수분해액 중 가장 양이 많을 뿐만 아니라 중대한 에탄올 발효 저해물질이다. 그 가수분해액으로부터 초산을 선택적으로 제거하는데 최적인 분리시스템을 찾기 위하여 모사 헤미셀룰로오스 가수분해액으로 자일로스, 초산과 황산의 혼합물이 선택되었다. 이러한 목적을 이루기 위해 에멀젼형 액막법이 모사 헤미셀룰로오스 가수분해액으로부터 초산을 제거하는데 적용되었다. W/O 에멀젼의 주요 구성 성분들, 즉 아민계 추출제 종류, 계면활성제 조성, 첨가제 종류와 회수제의 종류 및 농도가 모사 헤미셀룰로오스 가수분해액에 있는 초산, 자일로스와 황산의 추출에 미치는 영향이 조사되었다. 특별한 실험 조건에서, 초산의 추출율은 95% 이상이었고, 자일로스의 손실은 미미하였는데, 이것은 현 에멀젼형 액막법이 경제성이 높은 공정이 될 수 있다는 것을 의미한다.

• Mycobiology
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• 제37권2호
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• pp.133-140
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• 2009

To optimally convert corn hull, a byproduct from corn processing, into bioethanol using Pachysolen tannophlius, we investigated the optimal conditions for hydrolysis and removal of toxic substances in the hydrolysate via activated carbon treatment as well as the effects of this detoxification process on the kinetic parameters of bioethanol production. Maximum monosaccharide concentrations were obtained in hydrolysates in which 20 g of corn hull was hydrolyzed in 4% (v/v) $H_2SO_4$. Activated carbon treatment removed 92.3% of phenolic compounds from the hydrolysate. When untreated hydrolysate was used, the monosaccharides were not completely consumed, even at 480 h of culture. When activated carbon.treated hydrolysate was used, the monosaccharides were mostly consumed at 192 h of culture. In particular, when activated carbon-treated hydrolysate was used, bioethanol productivity (P) and specific bioethanol production rate ($Q_p$) were 2.4 times and 3.4 times greater, respectively, compared to untreated hydrolysate. This was due to sustained bioethanol production during the period of xylose/arabinose utilization, which occurred only when activated carbon-treated hydrolysate was used.

• 한국식품영양과학회지
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• 제22권4호
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• pp.470-475
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• 1993

대구피 젤라틴을 효소적으로 가수분해하여 얻어진 가수분해물의 분자량 분포 및 아미노산 조성을 살펴보고 이 가수분해물을 이용해 조미간장을 제조하였고 그 품질을 시판 간장과 관능적으로 비교, 검토하였다. 가수분해물의 분자량은 5,800Da영역이 주종이었으며 분자량 1,100Da, 1,500Da및 2,700Da정도의 펩티드성 분자도 존재하였다. 아미노산은 단맛을 내는 아미노산(glycine, proline, serine, alanine 및 hydroxypro-line)과 감칠맛, 신맛을 내는 아미노산(glutamic acid, aspartic acid)이 전체의 65.9%를 차지하고 있었다. 반면 쓴맛을 내는 아미노산(arginine, tyrosine, phenylala-nine, valine, leucine, methionine 및 histidine)은 전체의 26.65%에 불과하였다. 가수분해물 10.0g,식염 10.0g, 설탕 3.0g, MSG 0.5g, 카라멜분말 0.1g, 양조식초 3.0$m\ell$, 마늘분말 0.05g, 검은 후추분말 0.1g 및 감초분말 0.2g을 물 100.0$m\ell$에 용해하여 열처리한 다음 여과하여 얻어진 원액과 시판 양조간장을 8 : 2(v:v)의 비율로 혼합하여 제조한 조미간장은 시판 3종류의 화학간장과 비교해 관능적으로 손색이 없었다.

• Journal of Microbiology and Biotechnology
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• 제23권12호
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• pp.1779-1784
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• 2013

Although enzyme-hydrolyzed silk fibroin has been reported to enhance cognitive function before, it has been still unknown which peptides can improve memory. Here we report that amino acid sequences of three novel peptides were identified from fibroin hydrolysate. Fibroin hydrolysate was obtained by hydrolysis with protease after partial hydrolysis with 5M $CaCl_2$. Synthesized peptides derived from these sequences improved scopolamine-induced memory impairments in mice. We confirmed this hydrolysate had effects that improved learning and memory abilities by performing the Rey-Kim test. From this hydrolysate of silk fibroin, amino acid sequences of eight peptides were identified by LC-MS/MS. Three peptides (GAGAGTGSSGFGPY, GAGAGSGAGSGAGAGSGAGAGY, and SGAGSGAGAGSGAGAGSGA) were synthesized to investigate whether they could improve memory. Passive avoidance test and Morris water maze test were performed, and all peptides showed memory-enhancing abilities on scopolamine-induced memory impairments in mice. In this study, we identified three novel peptides that could improve memory, and that silk fibroin hydrolysate was a mixture of various active peptides that could enhance memory.

• KSBB Journal
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• 제15권6호
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• pp.635-643
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• 2000

To compare the antioxidative activities of fish skin and bovine skin gelatin hydrolysate, gelatin hydrolysates from Alaska pollack and bovine skin were prepared by various enzymatic hydrolysis methods (1st step, Alcalase; 2nd step, pronase E; 3rd step, collagenase) using a continuous three-step membrane reactor. The molecular weight distributions of the 1st, 2nd and 3rd step hydrolysates were 7∼10 kDa, 2∼5 kDa and 0.7∼0.9 kDa, respectively. The antioxidative activity of fish skin gelatin hydrolysate was stronger than that of bovine skin gelatin hydrolysate, and in particular, both of 2nd step hydrolysates showed more antioxidative activity than hydrolysates of any other step. The optimum antioxidative activity concentration of the 2nd step hydeolysates of fish and boving skin were 1% (w/w) in a linoleic acid water-alcohol emulsion. In cultured cells exposed to t-butyl hydroperoxide (t-BHP), the 2nd step hydrolysate of fish skin gelatin delayed cell death most. These results suggest that the antioxidative activity of fish skin gelatin hydrolysate is higher than that of bovine skin gelatin hydrolysate because of their different amino acid contents.

• 현장농수산연구지
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• 제14권1호
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• pp.93-105
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• 2012

서해안 멸치를 이용한 저염 천연조미료를 생산하고자 시판 효소 3종을 사용하여 효소가수분해물을 제조하고 이의 품질특성을 파악하였다. 멸치 효소가수분해물의 주요 유리아미노산은 글루타민산과 라이신이었으며, 전체 유리아미노산의 함량은 글루타민산(12.6%), 리신(8.56%), 발린(7.06%), 아스파탐(5.73%)순으로 나타났다. 휘발성 정미성분은 전체 30개의 성분이 검출되었으며, aldehyde, ketone, cyclic compound 등으로 나타났다. 가수분해물 제조과정 중 히스타민 함량의 변화는 17.1-18.1 mg/100g으로 변패에 대해 안전한 수준이었다.