• 제목/요약/키워드: accumulation rate

검색결과 1,064건 처리시간 0.04초

Enhancement of eurycomanone biosynthesis in cell culture of longjack (Eurycoma longifolia) by elicitor treatment

  • Nhan, Nguyen Huu;Loc, Nguyen Hoang
    • Journal of Plant Biotechnology
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    • 제45권4호
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    • pp.340-346
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    • 2018
  • In this study, the effect of elicitors such as yeast extract (YE), methyl jasmonate (MeJA) and salicylic acid (SA) on the accumulation of eurycomanone in Eurycoma longifolia cell cultures were investigated. Suspension cells of E. longifolia was cultured in Murashige and Skoog (MS) medium supplemented with 30 g/L sucrose, 1.25 mg/L naphthaleneacetic acid (NAA) and 1 mg/L kinetin at a shaking speed of 120 rpm. Elicitors were added in the culture at different concentrations and times to stimulate eurycomanone accumulation in the Eurycoma longifolia cells. Eurycomanone content was determined by HPLC with a C18 column, flow rate of 0.8 mL/min, run time of 17.5 min, and a detector wavelength of 254 nm. The stationary phase was silica gel and the mobile phase was acetonitrile: $H_2O$. Non-elicited cells were used as the control. The study showed the effect of different elicitor concentrations, YE at 200 mg/L, MeJA at $20{\mu}M$ and SA at $20{\mu}M$ stimulated high production of eurycomanone. In which, treatment of $20{\mu}M$ MeJA after 4 days of culture resulted in the highest accumulation of this compound (17.36 mg/g dry weight), approximately 10-fold higher than that of untreated cells (1.70 mg/g dry weight).

왕겨연소기(燃燒機)의 성능(性能)에 영향(影響)을 마치는 설계(設計) 및 작동인자(作動因子)에 관(關)한 연구(硏究) (The Effects of Operational and Mechanical Factors on the Performance of Rice-Husk Furnace)

  • 박승제;노상하
    • Journal of Biosystems Engineering
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    • 제8권2호
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    • pp.39-48
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    • 1983
  • This study was performed to obtain the basic data which could be used for the modification of the manual center-burner-type rice-husk furnace into a small scale automatic type for the multi-purpose use in the farm. For this purpose, first, the utilization feasibility of the rice-husk furnace in the farm was analyzed briefly in aspects of available amount of rice-husk for the fuel, annual operation time and replaceble amount of residential heating energy with rice-husk in the farm. For the experiment a prototype furnace geared with an automatic feeding device was fabricated, and feed rate, mold size and chimney height were changed to investigate the combustion efficiency of rice-husk and thermal efficiency of the furnace. Also, optimum and limiting operational factors were observed in each treatments. The results obtained are summarized as follows. 1. If the rice-husk is intensively used for residential heating in the farm for winter season, on an average 51 percent of the total heating energy can be replaced with the rice-husk. Therefore, development of a small scale automatic rice-husk furnace was recognized to be feasible. 2. The operational condition depending on husk-feed rates was very important factor for successive steady burning operation of the given furnace. When the feed-rate was 1.5 kg/hr, the top of the burning zone should be kept at the position about 55 cm from the bottom of the combustion chamber with the periodic removal of ash (termed as steady state position), which was 18 cm above the mold waist. When the feed rates were 2.4 kg/hr and 3.0 kg/hr, the steady state position was at about 4 cm above the mold waist. 3. The mold size affected inflow rate of air into the furnace and consequently CO content in the exhaust gas. The relatively bigger mold gave positive effect on the air-inflow rate. 4. When the husk-feed rates were 1.5 kg/hr, 2.4 kg/hr, 3.0 kg/hr, the combustion efficiencies of the rice-husk were 98.5%, 97.4% and 95.0%, the thermal efficiencies of the furnace were 93.4%, 93.2% and 87.6%, and CO content in the exhaust gas were 1.21%, 1.03%, and 2.43%, respectively. The air-inflow rates were decreased with the increase of feed rates. When the amount of excess air was 30-40%, the CO content in the exhaust gas was at the minimum level. 5. When the chimney height was lowered from 260 cm to 96 cm, the air-inflow rate was slightly decreased, but the average temperature in the combustion chamber, CO content in the exhaust gas and combustion and thermal efficiencies were not changed significantly. 6. The incidental problems associated with the protytype furnace were accumulation of the ash inside the mold, accumulation of the cinder between the outer-drum of the furnace and the combustion chamber wall, and accumulation of the cinder in the chimney.

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산화막 CMP에서 발생하는 온도가 연마특성에 미치는 영향 (Effect of Temperature on Polishing Properties in Oxide CMP)

  • 김영진;박범영;김형재;정해도
    • 한국전기전자재료학회논문지
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    • 제21권2호
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    • pp.93-98
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    • 2008
  • We investigated the effect of process temperature on removal rate and non-uniformity based on single head kinematics in oxide CMP. Generally, it has been known that the temperature profile directly transfers to the non~uniformity of removal rate on the wafer, which has similar tendency with the sliding distance of wafer. Experimental results show that platen velocity is a dominant factor in removal rate as well as average temperature. However, the non-uniformity does not coincide between process temperature and removal rate, due to slurry accumulation and low deviation of temperature. Resultantly, the removal rate is strongly dependent on the rotational speed of platen, and its non -uniformity is controlled by the rotational speed of polishing head. It means lower WIWNU (With-in-wafer-non-uniformity) can be achieved in the region of higher head speed.

미세조류 Botryococcus braunii의 배양조건 최적화 및 지질축적 향상 (Optimal Culturing and Enhancement of Lipid Accumulation in a Microalga Botryococcus braunii)

  • 권성현;이은미;조대철
    • 한국환경과학회지
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    • 제21권7호
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    • pp.779-785
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    • 2012
  • Several tests and experimental work have been done for identifying the best growth conditions and accumulated amount of lipid moiety in B. braunii, a microalga(UTEX 572) in terms of media composition. The specific growth rate was found to be the highest at 0.15 g/L-day when the phosphorus concentration was doubled with the other ingredients at the normal level. Experiments for varied media compositions revealed that the accumulation of lipid was the highest at 48% (dry cell weight based) in the nitrogen deficient medium and its corresponding specific growth rate was comparative to that in the normal BG 11 medium. In the bubble column experiments, carbon dioxide containing air produced four times more cell mass than air only. Light and glucose addition also enhanced cell mass with maximum, 1.8 g/L and accordingly 42% of lipid composition, which turned out to be a better strategy for higher lipid-producing microalgal culture.

신개간지에서 구비 및 삼요소시용이 청예사료작물의 생육 및 건물축적에 미치는 영향 (effect of Cattle Compost and NPK Application on Growth and Dry Matter Accumulation of Selected Forage Crops on Neqly Reclaimed Uplands)

  • 한민수;박종선
    • 한국초지조사료학회지
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    • 제11권2호
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    • pp.108-115
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    • 1991
  • A field experiment was conducted to evaluate the effects of cattle compost application on the change of soil physical properties and their relationship to yield performance of selected main forage crops. Maize(CV. Suweon 19) and sorghum hybrids(CV. Pioneer 9'31) as a summer crops and winter rye were grown on newly reclaimed red yellow soils(Fine loamy, Typic Hapludults) under different application rate of cattle compost associated with chemical NPK fertilization, from Oct. 1986 to Sept. 1989. Experimental field was laid down as a split plots design with four replications. The results obtained are summarized as follows: 1. Cattle compost application reclaimed soil physical propeties, such as formation of granular structure and water holding capacity, and it result in a great increase of plant growth and the rate of dry matter accumulation. 2. While cattle compost treatment reduced the portion of soild phase of the three phase constituents of soils, it increased the portion of air phase and liquid phase comparatively. 3. Organic matter, N, P, K, and mineral content in soil were markedly increased in the plot treated with compost. 4. Cattle compost application increased fodder production both in maize-rye and sorghum hybrids-rye cultivation. Annual dry matter yield of maize-rye cropping was 2183(NI'K only), 2425(NPK+compvst 3000 kg) and 2800kg/lOa(NPK + compost 6000kg/10a).

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Optimization of Influencing Factors on Biomass Accumulation and 5-Aminolevulinic Acid (ALA) Yield in Rhodobacter sphaeroides Wastewater Treatment

  • Liu, Shuli;Li, Xiangkun;Zhang, Guangming;Zhang, Jie
    • Journal of Microbiology and Biotechnology
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    • 제25권11호
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    • pp.1920-1927
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    • 2015
  • This study aimed to optimize four factors affecting biomass accumulation and 5-aminolevulinic acid (ALA) yield together with pollutants removal in Rhodobacter sphaeroides wastewater treatment. Results showed that it was feasible to produce biomass and ALA in R. sphaeroides wastewater treatment. Microaerobic, 1,000-3,000 lux, and pH 7.0 were optimal conditions for the highest ALA yield of 4.5 ± 0.5 mg/g-biomass. Under these conditions, COD removal and biomass production rate were 93.3 ± 0.9% and 31.8 ± 0.5 mg/l/h, respectively. In addition, trace elements Fe2+, Mg2+, Ni2+, and Zn2+ further improved the ALA yield, COD removal, and biomass production rate. Specifically, the highest ALA yield (12.5 ± 0.6 mg/g-biomass) was achieved with Fe2+ addition.

주거환경개선사업지구의 필지개발 방안에 관한 연구 - 개별필지집적방식에 의한 노인복지공동주택사업의 적용가능성을 중심으로 - (The research of estate development scheme in residential environment improvement)

  • 정은정;이희진;양병옥;김주석;이연숙
    • 한국주거학회:학술대회논문집
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    • 한국주거학회 2008년 추계학술발표대회 논문집
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    • pp.235-240
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    • 2008
  • When rate of stated and public owned demesne in residential environment improvement is low under the limited resources of revenues, the new infrastructure for the maintenance of the existing estate has increased land reduction rate thus the place where lot of small estate find it difficult to accept as the feature of residential environment improvement. Although 'Diffusion model of residential environment improvement' which designed to overcome existing residential environment improvement, is middle of progress, there is not any suggestion made to solve problem caused by short of estate in order to maintain small estate and total Infrastructure. In feature of resident, we also notice ageing not only facilities and building. 'Elderly housing' can be presented as an alternative living style to solve housing and elderly matter which are caused by accumulation of individual estate and aging at once. Therefore, this research identifies resident's perception about elderly housing and recognizes task and potentialities that has to be solved in order to apply virtually to residential environment improvement.

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Distribution of Cd, Cu and Zn in a Sewage Sludge-treated Calcareous Soil

  • Lee, Sang-Mo;Cho, Chai-Moo;Yoo, Sun-Ho
    • Journal of Applied Biological Chemistry
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    • 제42권3호
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    • pp.134-139
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    • 1999
  • The distributions of Cd, Cu, and Zn concentration in soil treated with one (1988) or two (1988 and 1993) applications of sewage sludge at rates of 0, 25, 50, and $100Mg\;ha^{-1}$ (dry weight basis) were determined to assess the accumulation and mobility of the heavy metals. The heavy metals accumulated almost entirely in 0 to 15 cm soil depths. Small amounts of the metals moved out of the tillage zone (0-15 cm depth) into the subsoil, but even at the high rate of sewage sludge, little movement of heavy metals occurred below 100 cm depth. The water-extractable Cd, Cu, and Zn concentrations were very low regardless of the rate of sewage sludge application. Availability of metals as determined by DTPA extraction showed the percentage of DTPA-extractable/total concentration increased with sewage sludge application. In the 0-15 cm depth of sewage sludge treated soil, the percentage of DTPA-extractable/total concentration was higher than 46% for Cd, but the value was less than 27% and 17% for Cu and Zn, respectively. The Cd, Cu, and Zn added to this calcareous clay soil by sewage sludge application were not very mobile, and the amount of plant available form was very small.

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식물에 미치는 방사성 동위원소 S35의 영향에 대하여 (제3보) 발아호밀의 Amylase Activity 및 생장에 미치는 온도의 영향에 대하여 (Effects of absorbed radioactive sulfur (S35) in plant cell.(III) Effects of temperatures on amylase activity and growth of rye seedlings grown in solution of S35)

  • 홍순우
    • Journal of Plant Biology
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    • 제11권1호
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    • pp.1-6
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    • 1968
  • The effects of the different temperatures on the amylase activity and growth rate of the rye seedling grown in the solutions containing radioactive sulfur- 35 were studied. The amylase activity of the coleoptiles obtained from the seedlings grown in the solutions of S-35, at 14$^{\circ}C$, appeared to be strongly stimulated in comparison to the control, but the culture temperatures of 22$^{\circ}C$ and 3$0^{\circ}C$ showed the decrease in the amylase activity. The amylase activity of the grains treated with the low intensity of the ratioactive material didn't show clear changes, at any culture temperatures, but the amylase activity of the grains treated with the high intensity of S-35, 50$\mu$c, showed definite decline at the elevated culture temperature, 3$0^{\circ}C$. Similar effects was also found in the growth of the seedlings. However, we would consider the effects of the radioactive materials on the acticity of the amylase and the growth of the seedlings are resulted from the accumulation of the much amount of the radioactive materials, and this accumulation rate depends upon actually the elevation of the culture temperature.

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Chitosan과 Jasmonic acid 처리에 의한 인삼 부정근의 Ginsenosides의 생산성 증대 (Increase of Ginsenosides Production by the Treatment of Chitosan and Jasmonic Acid in the Adventitious Roots of Korean Ginseng (Panax ginseng C.A. Meyer))

  • 이범수;인준교;송원섭;양덕춘
    • 한국자원식물학회지
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    • 제17권1호
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    • pp.48-53
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    • 2004
  • 인삼 부정근의 생장과 ginsenosides 함량에 미치는 elicitor의 영향을 조사한 결과 chitosan처리구의 생장량은 10 mg/L에서 가장 양호하였으며, ginsenosides의 함량은 5 mg/L처리농도에서 14.48 mg/g $.$ DW로 대조구에 비해 약9%의 함량증대를 가져왔다. 세포내에서 이차대사계를 활성화시키는 중간 신호전달 물질(signal transducer)로 알려진 jasmonic acid를 인삼 부정근 배양에 처리한 결과 부정근의 생장량은 10 uM 처리구에서 ginsenosides함량이 가장 양호하였으나, 인삼 부정근의 생장은 다소 억제되었다.