• 한국약용작물학회지
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• 제18권6호
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• pp.405-408
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• 2010

Some fatty acids(palmitic acid, stearic acid, oleic acid, linoleic acid and linolenic acid) in foxtail millet seeds were evaluated. In this study, 32 varieties of accession collected from different place of South Korea were used in order to evaluate their fatty acid composition and concentration. These samples were analyzed by GC with HP20 column. Significant variability in individual fatty acids was observed. The palmitic acid ranged from 10.9 to 13.5%, stearic acid from 2.5 to 8.3%, oleic acid from 10.0 to 15.8%, linoleic acid from 62.5 to 67.9% and linolenic acid ranged from 3.0 to 5.2%. This variability can be exploited for selection of plant and for studying the genetic variability in millet. These selections can also be utilized for genetic improvement of the crops.

• Preventive Nutrition and Food Science
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• 제9권4호
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• pp.324-329
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• 2004

In order to investigate the molecular mechanism of $\gamma$-aminobutyric acid (GABA) production in lactic acid bacteria, we cloned a glutamate decarboxylase (GAD) gene from Lactobacillus plantarum using polymerase chain reaction (PCR). One PCR product DNA was obtained and inserted into a TA cloning vector with a T7 promoter. The recombinant plasmid was used to transform E. coli. The insertion of the product was con­firmed by EcoRI digestion of the plasmid purified from the transformed E. coli. Nucleotide sequence analysis showed that the insert is a full-length Lactobacillus plantarum GAD and that the sequence is $100\%$ and $72\%$ identical to the regions of Lactobacillus plantarum GAD and Lactococcus lactis GAD sequences deposited in GenBank, accession nos: NP786643 and NP267446, respectively. The amino acid sequence deduced from the cloned Lactobacillus plantarum GAD gene showed $100\%$ and $68\%$ identities to the GAD sequences deduced from the genes of the NP786643 and NP267446, respectively. To express the GAD protein in E. coli, an expression vector with the GAD gene (pkk/GAD) was constructed and used to transform the UT481 E. coli strain and the expression was confirmed by analyzing the enzyme activity. The Lactobacillus plantarum GAD gene obtained may facilitate the study of the molecular mechanisms regulating GABA metabolism in lactic acid bacteria.

• 대한수의학회지
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• 제44권4호
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• pp.571-579
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• 2004

In order to obtain the genetic informations of the Korean isolates of porcine circovirus 2 (PCV2), nucleotide sequences of total genome of three isolates and open reading frame 2 (ORF2) of four isolates were determined and compared with those of other reference PCV2 isolates. Nucleotide sequences of 3 isolates showed over 99% homology with those of reference strain (GenBank accession no. AF027217). Point mutations were mainly determined on ORF2 regions but little on ORF1 regions. The patterns of pointmutated sites and nucleotide substitution on ORF2 regions were generally consistent between Korean isolates, and these mutated sites observed in Korean isolates were also relatively similar to those of foreign isolates. Phylogenetic analysis of nucleotide or amino acid sequences showed that there were minor branches consisting of three clusters; cluster of Korea, Canada and America, cluster of Spain and Taiwan, and the last cluster of French and China isolates. These results suggested that Korean PCV2s were probably originated from North America such as Canada or USA. The genetic informations obtained from this study could be useful for the research of diagnosis and pathogenecity of PCV2.

• 한국문헌정보학회지
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• 제31권2호
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• pp.79-93
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• 1997

동일 분류항목 내에 분류되는 저작들을 개별화하여 배열과 검색을 용이하게 하기 위해 사용되는 도서기호는 크게 입수순기호, 저자기호, 그리고 연대순기호로 나누어 볼 수 있다. 현대의 매우 빠른 학문 발전속도는 최신의 정보가 최상의 가치를 갖도록 만들었고, 이용자들의 이용행태 또한 최신의 자료에 집중되고 있다. 이러한 정보의 가치기준과 이용행태의 변화에 따라 도서관에서는 최신의 자료를 오래된 것과 구분하여 모아줄 필요가 발생하였다. 본고에서는 이러한 요구를 반영하여 고안된 「새 연대순 도서기호법」이 널리 채용되게 하고자 하는 목적으로 그 특징과 기호구성을 살펴보았고, 일부 자모순 도서기호를 적용토록 지시된 항목의 성격에 대해 규명하였다.

• 기록학연구
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• 제10호
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• pp.112-144
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• 2004

Oral history has value that steps over the class consciousness and party feeling that records have, and enables a general representation of a human experience. Beside, It must be managed urgently because a collection of oral history has unique and limited. However, domestic related institution do not keep management for release and use of oral history in their mind are only concentrating on accession. Lately, National History Compilation Committee passes through faithful planning procedures and is collecting an oral history collection project. A merit of this project is to have attempted an image photographing. This study is propose that to examines over the all steps to say oral history collection activity to information service and a preservation from collection planning of organization. However, this study is insufficient in the management method how it is connected if does in order to manage the oral history which is collected with a part of a record in a archival institution because this study was only for the purpose of management of oral history. This problem will be studied through a process of checking the real thing of oral history. This study proposed general methodology for oral history management, if institution had a plan of collection and management or had some collection result.

• 무역학회지
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• 제45권1호
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• pp.137-153
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• 2020

On February 14, 2019, the government of Korea formally decided to consider the feasibility of joining the Comprehensive and Progressive Trans-Pacific Partnership (CPTPP) and has since been conducting bilateral consultations with individual member countries. In terms of the impact estimation, the CPTPP is actually a Korea-Japan FTA, and the most sensitive issue in the FTA is the opening of the auto industry market to Japan. Despite these circumstances, previous studies have predicted that the auto industry will be a beneficiary industry when joining the CPTPP. However, the Korean auto industry is opposed to joining the CPTPP. In order to investigate the cause of this discrepancy, this paper examines the problems of previous studies in estimating the impact of joining the CPTPP and found that the preceding study did not consider the industrial characteristics of the auto sector, especially in the context of Japan-Korea trade, and was heavily dependent on the Armington elasticity (structure) in the demand function of the GTAP CGE model. As a result, the domestic auto sector could lower prices and increase exports when joining the CPTPP. This paper attempts to precisely re-estimate the impact of joining the CPTPP on the auto sector in a way that corrects these problems by changing the CGE model and reflecting the major characteristics of the industry, with policy implications for the negotiation of CPTPP accession.

• 미생물학회지
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• 제32권3호
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• pp.177-181
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• 1994

담자균류 균심류의 영지과에 속하는 잔나비걸상(Ganoderma applanatum)과 구멍장이 버섯과에 속하는 좀구멍버섯 (Schizopora paradoxa) 두 종의 5S rRNA 염기서열들을 (EMBL accession mumbers X73589 and X73890) directchemical method로 분석 결정하고 담자균류 균심류와 복균류의 기존에 밝혀진 9종 버섯의 염기서열과 비교하였다. 잔나비걸상과 좀구멍버섯의 5S rRNA는 각각 118개의 염기로 구성되어 있으며 B형 5S rRNA에 해당하였고 Huysmans 등이 제시한 2차구조의 모델에 들어 맞으며 Walker와 Doolittle이 제시한 제 5 염기서열군에 속하였다. 진화거리를 나타내는 Kimura의 염기치환상수 $K_{nuc}$값에 으하면 잔나비걸상과 가장 가까운 좋은 고약버석과의 Ceratobasidium cornigerum으로서 염기 3개의 차이를 보였으며 좀구멍버섯과 가장 가까운 좋은 구멍장이 보섯과의 줄버섯(Bjerkandera adusta)으로서 염기 두개의 차이를 보였다.11개 5S rRNA의 이차구조를 비교하였을때 염기의 치환은 loop 부분보다는 helix 부분에서 많이 일어났으며, 이는 helix 부분이 loop 부분보다는 진화적으로 덜 보존되어 있고 진화 분지를 형성하는데 보다 많은 영향을 주었음을 시사하였다. Kimura의 two parameter method로 계산된distance matrix를 사용하고 Felsenstein PHYLIP package의 Neighbor program에서 Neighborjpomomg option을 이용하여 계통수를 그렸을 때 균심류의 버섯들은 부분적으로 목별로 구분되었다. 균심류의 민주름버섯목에는 적어도 먹물버섯류(Coprinus radiatus)를 제외한 2개의 계통분지가 있고 주름버섯목에도 2개의 계통 분지가 있으며, 복균류에는 말불버섯(Lycoperdon pyriforme)이 독립된 계통분지를 형성하고 있음을 시사하였다.

• 한국비블리아학회지
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• 제2권1호
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• pp.102-131
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• 1974

The author's thesis is that the types used for the large-sized characters seen in the two metal type-printed books "Kyosik chubopop karyong"(交食推步法假令) and "Yok-hak kemong yohae"(易學啓蒙要解) both printed in 1458 belong to a new metal font hitherto unnamed. The former book was compiled by Yi Sun-ji(李純之) and Kim Sok-je(金石梯) in January of 1458 in accordance to King Sejo's order. A new font was created to be used for the large-sized characters of the book. Several. months after completion of the compilation, the book was printed with mixed use of the new font and the Kabin-ja(甲寅字) for medium- and small-sized characters. The latter book had been written by King Sejo before his accession to the throne. Ascending the throne the king had his scholar-subjects examine the writing to correct it where necessary. The examination was completed in July of 1458 and printing was immediately done with the two fonts the above-mentioned, new font for the large-sized letters and the Kabin-ja for the medium- and small-sized ones. The books were granted to the scholar-subjects and the students of the Sung Kyun Kwan Academy as a royal gift. The matrix seems to have been modeled after the calligraphy of King Sejo. Because the new font was created to print the large-sized letters of the two books in 1458, it may be proper to name it "Muin-ja" using the "kanji"(干支) of the year. The author is happy to identify and include another font in the list of Korean movable types as a result of the present study.

• Journal of Microbiology and Biotechnology
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• 제20권1호
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• pp.208-216
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• 2010

Fusarium verticillioides is an important pathogen of maize, being responsible for ear rots, stalk rots, and seedling blight worldwide. During the past decade, F. verticillioides has caused several severe epidemics of maize seedling blight in many areas of China, which lead to significant losses. In order to understand the molecular mechanisms regulating fungal development and pathogenicity in this pathogen, we isolated and characterized the gene fpk1 (GenBank Accession No. EF405959) encoding a homolog of the cAMP-dependent protein kinase catalytic subunit, which included a 1,854-bp DNA sequence from ATG to TAA, with a 1,680-bp coding region, and three introns (lengths: 66 bp, 54 bp, and 54 bp), and the predicated protein precursor had 559 aa. The mutant ${\Delta}fpk1$, which was disrupted of the fpkl gene, showed reduced vegetative growth, fewer and shorter aerial mycelia, strongly impaired conidiation, and reduced spore germination rate. After germinating, the fresh hypha was stubby and lacking of branch. When inoculated in susceptible maize varieties, the infection of the mutant ${\Delta}fpk1$ was delayed and the infection efficiency was reduced compared with that of the wild-type strain. AU this indicated that gene fpk1 participated in hyphal growth, conidiophore production, spore germination, and virulence in F. verticillioides.

• Asian-Australasian Journal of Animal Sciences
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• 제25권5호
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• pp.606-612
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• 2012

Insulin-like growth factor-binding protein-5 (IGFBP-5) is one of the six members of IGFBP family, important for cell growth, apoptosis and other IGF-stimulated signaling pathways. In order to explore the significance of IGFBP-5 in cells of the Inner Mongolian Cashmere goat (Capra hircus), IGFBP-5 gene complementary DNA (cDNA) was amplified by reverse transcription polymerase chain reaction (RT-PCR) from the animal's fetal fibroblasts and tissue-specific expression analysis was performed by semi-quantitative RT-PCR. The gene is 816 base pairs (bp) in length and includes the complete open reading frame, encoding 271 amino acids (GenBank accession number JF720883). The full cDNA nucleotide sequence has a 99% identity with sheep, 98% with cattle and 95% with human. The amino acids sequence shares identity with 99%, 99% and 99%, respectively. The bioinformatics analysis showed that IGFBP-5 has an insulin growth factor-binding protein homologues (IB) domain and a thyroglobulin type-1 (TY) domain, four protein kinase C phosphorylation sites, five casein kinase II phosphorylation sites, three prenyl group binding sites (CaaX box). The IGFBP-5 gene was expressed in all the tested tissues including testis, brain, liver, lung, mammary gland, spleen, and kidney, suggesting that IGFBP-5 plays an important role in goat cells.