• Title/Summary/Keyword: accessibility evaluation

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Bactericidal Effect of Cecropin A Fused Endolysin on Drug-Resistant Gram-Negative Pathogens

  • Lim, Jeonghyun;Hong, Juyeon;Jung, Yongwon;Ha, Jaewon;Kim, Hwan;Myung, Heejoon;Song, Miryoung
    • Journal of Microbiology and Biotechnology
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    • v.32 no.6
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    • pp.816-823
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    • 2022
  • The rapid spread of superbugs leads to the escalation of infectious diseases, which threatens public health. Endolysins derived from bacteriophages are spotlighted as promising alternative antibiotics against multi-drug resistant bacteria. In this study, we isolated and characterized the novel Salmonella typhimurium phage PBST08. Bioinformatics analysis of the PBST08 genome revealed putative endolysin ST01 with a lysozyme-like domain. Since the lytic activity of the purified ST01 was minor, probably owing to the outer membrane, which blocks accessibility to peptidoglycan, antimicrobial peptide cecropin A (CecA) was fused to the N-terminus of ST01 to disrupt the outer membrane. The resulting CecA::ST01 has been shown to have increased bactericidal activity against gram-negative pathogens including Pseudomonas aeruginosa, Klebsiella pneumoniae, Acinetobacter baumannii, Escherichia coli, and Enterobacter cloacae and the most affected target was A. baumannii. In the presence of 0.25 µM CecA::ST01, A. baumannii ATCC 17978 strain was completely killed and CCARM 12026 strain was wiped out by 0.5 µM CecA::ST01, which is a clinical isolate of A. baumannii and resistant to multiple drugs including carbapenem. Moreover, the larvae of Galleria mellonella could be rescued up to 58% or 49% by the administration of CecA::ST01 upon infection by A. baumannii 17978 or CCARM 12026 strain. Finally, the antibacterial activity of CecA::ST01 was verified using 31 strains of five gram-negative pathogens by evaluation of minimal inhibitory concentration. Thus, the results indicate that a fusion of antimicrobial peptide to endolysin can enhance antibacterial activity and the spectrum of endolysin where multi-drug resistant gram-negative pathogens can be efficiently controlled.

Design and Implementation of Biometrics Security System Using photoplethysmogram (광용적맥파를 이용한 생체인식 보안시스템의 설계 및 구현)

  • Kim, Hyen-Ki
    • Journal of Korea Society of Industrial Information Systems
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    • v.15 no.4
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    • pp.53-60
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    • 2010
  • Biometrics are methods of recognizing a person based on the physiological or behavioral characteristics of his of her body. They are highly secure with little risk of loss or falsification by others. This paper has designed and implemented a security system of biometrics by precisely measuring heartbeat signals at two fingertips and using a photoplethysmogram, which is applicable to biometrics. A performance evaluation has led to the following result. The security system of biometrics for personal authentication which has been designed and implemented by this study has achieved a recognition rate of 90.5%. The security system of biometrics suggested here has merits of time saving and easy accessibility. The system is touch-based and collects the necessary biometrics information by simply touching the machine with fingers, so anyone can utilize the system without any difficulty.

Evaluation of Network Reshuffling Alternatives Based on Key Factors Affecting the Mode Share of Seoul Metro (서울시 도시철도 이용에 영향을 미치는 요소를 반영한 노선 조정 효과 분석)

  • Jo, Dohyoung;Sohn, Keemin;Kim, Daehyun;Kim, Ikki
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.26 no.6D
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    • pp.935-943
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    • 2006
  • Key factors affecting the mode share of Soul metro are investigated. The log-regression model, which can describe the elasticity of the factors with ease, is established rather than the conventional mode choice model is used. The log-regression model requires lower level of data availability for calibration and identifies the impact of the factors on mode share straightforwardly. As a result, it is found that the main reasons why the current mode share of railway is low are due to several problems such as winding lines, inconvenient transfers and unnecessary bypasses. The calibrated model is adopted to evaluate the network reshuffling alternatives. The network reshuffling is to rearrange the existing inefficient railway lines that have frequent transfers and many winding segments. The proposed network reshuffling, which includes straightening winding lines and changing grade separated transfers into cross-platform transfers, turned out to be a good measure to tackle the problems.

Quantifying the 2022 Extreme Drought Using Global Grid-Based Satellite Rainfall Products (전지구 강수관측위성 기반 격자형 강우자료를 활용한 2022년 국내 가뭄 분석)

  • Mun, Young-Sik;Nam, Won-Ho;Jeon, Min-Gi;Lee, Kwang-Ya;Do, Jong-Won;Isaya Kisekka
    • Journal of The Korean Society of Agricultural Engineers
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    • v.66 no.4
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    • pp.41-50
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    • 2024
  • Precipitation is an important component of the hydrological cycle and a key input parameter for many applications in hydrology, climatology, meteorology, and weather forecasting research. Grid-based satellite rainfall products with wide spatial coverage and easy accessibility are well recognized as a supplement to ground-based observations for various hydrological applications. The error properties of satellite rainfall products vary as a function of rainfall intensity, climate region, altitude, and land surface conditions. Therefore, this study aims to evaluate the commonly used new global grid-based satellite rainfall product, Climate Hazards Group InfraRed Precipitation with Station data (CHIRPS), using data collected at different spatial and temporal scales. Additionally, in this study, grid-based CHIRPS satellite precipitation data were used to evaluate the 2022 extreme drought. CHIRPS provides high-resolution precipitation data at 5 km and offers reliable global data through the correction of ground-based observations. A frequency analysis was performed to determine the precipitation deficit in 2022. As a result of comparing droughts in 2015, 2017, and 2022, it was found that May 2022 had a drought frequency of more than 500 years. The 1-month SPI in May 2022 indicated a severe drought with an average value of -1.8, while the 3-month SPI showed a moderate drought with an average value of 0.6. The extreme drought experienced in South Korea in 2022 was evident in the 1-month SPI. Both CHIRPS precipitation data and observations from weather stations depicted similar trends. Based on these results, it is concluded that CHIRPS can be used as fundamental data for drought evaluation and monitoring in unmeasured areas of precipitation.

Current Status and Direction of Generative Large Language Model Applications in Medicine - Focusing on East Asian Medicine - (생성형 거대언어모델의 의학 적용 현황과 방향 - 동아시아 의학을 중심으로 -)

  • Bongsu Kang;SangYeon Lee;Hyojin Bae;Chang-Eop Kim
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.38 no.2
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    • pp.49-58
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    • 2024
  • The rapid advancement of generative large language models has revolutionized various real-life domains, emphasizing the importance of exploring their applications in healthcare. This study aims to examine how generative large language models are implemented in the medical domain, with the specific objective of searching for the possibility and potential of integration between generative large language models and East Asian medicine. Through a comprehensive current state analysis, we identified limitations in the deployment of generative large language models within East Asian medicine and proposed directions for future research. Our findings highlight the essential need for accumulating and generating structured data to improve the capabilities of generative large language models in East Asian medicine. Additionally, we tackle the issue of hallucination and the necessity for a robust model evaluation framework. Despite these challenges, the application of generative large language models in East Asian medicine has demonstrated promising results. Techniques such as model augmentation, multimodal structures, and knowledge distillation have the potential to significantly enhance accuracy, efficiency, and accessibility. In conclusion, we expect generative large language models to play a pivotal role in facilitating precise diagnostics, personalized treatment in clinical fields, and fostering innovation in education and research within East Asian medicine.

Enhancing anti-calcification efficacy in veterinary cardiovascular surgeries: evaluating short-term ethanol's role in glutaraldehyde fixed pericardial tissues in rats

  • Kyung-Min Kim;Won-Jong Lee;Woo-Jin Kim;Chang-Hwan Moon;Jaemin Jeong;Hae-Beom Lee;Seong-Mok Jeong;Dae-Hyun Kim
    • Korean Journal of Veterinary Research
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    • v.64 no.2
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    • pp.16.1-16.9
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    • 2024
  • Autologous pericardial tissues are utilized in veterinary cardiovascular surgeries due to their accessibility and effectiveness. To enhance handling and biomechanical properties, glutaraldehyde (GA) fixation is applied. However, GA fixation can induce calcification, leading to tissue failure. This study aimed to establish an optimal rapid anti-calcification protocol by integrating ethanol treatment with the proven effective GA concentration and fixation time, facilitating application from collection to utilization. Pericardia were fixed with 0.625% GA for 20 min and subjected to ethanol treatment for 0 (group A, control), 20 (group B), and 30 minutes (group C). The treated tissues underwent mechanical test and were implanted subcutaneously in 3-week-old male rats for 7 weeks before extraction, followed by calcium analysis and histological examination via hematoxylin and eosin staining. No significant differences in mechanical properties were observed among the groups. The ethanol-treated groups (groups B and C; p < 0.05) exhibited significantly lower calcium levels than control (group A). Microscopy confirmed collagen and elastic fibers preservation, without significant immune cell variance. However, higher fibrocyte presence was noted in the ethanol-treated groups. This study presents a rapid anti-calcification protocol combining ethanol treatment with optimal GA fixation, suitable for direct surgical use of autologous tissues. Further research is necessary for long-term efficacy evaluation.

An Assessment on the Urban Riverfront in Shincheon, Daegu - Focused on a Universal Design Concept - (대구광역시 신천의 친수공간 평가 연구 - 유니버설디자인 개념을 중심으로 -)

  • Choi, Dong-Sik;Moon, Ji-Won;Kim, Shang-Hee
    • Journal of the Korean Institute of Landscape Architecture
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    • v.40 no.2
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    • pp.1-14
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    • 2012
  • The purpose of this study is to derive a desirable riverfront construction plan to me for the activities of citizens through the evaluation and analysis of the urban riverfront space from the perspective of universal design. Therefore, previous studies were examined in order to induce evaluation tools that bhve been grafted from the universal design concept; in addition, a field survey was conducted in Shincheon, Daegu, which was selected as the study target, in order to induce problems and improvement directions from the perspective of universal design. The major results can be summarized as follows. (1) In the 'fairness' aspect, all the items such as installation of integrated functional signage, showing pictures, symbols, foreign language signs, and restroom entrances signage were determined to be 'All Unsuitable' for all sections; and therefore, it is necessary to improve the fairness of usage for everybody. (2) In the 'Functionality(Usability)' aspect, all items such as installation of bicycle paths beside access roads, installation of integrated functional signs, and night light signs were determined to be 'All Unsuitable' for all sections; therefore, it is necessary to improve the functionalities of these facilities. (3) In the 'Convenience' aspect, all items such as the installation of bicycle parking areas, continuous rest facilities, and back and ann support(handles) at resting facilities were determined to be 'All Unsuitable' for many sections; therefore, it is necessary to improve these facilities for the convenience of usage. (4) In the 'Information(Recognizability)' aspect, all items such as showing pictures, symbols, foreign languages and installation of night light signs, and restroom entrances signage were determined to be 'All Unsuitable' for all sections; therefore, it is necessary to improve the recognizability to minimize misunderstandings and confusion. (5) In the 'Safety' aspect, all items such as the installation of safe pedestrian paths in parking areas, using anti-slip and shock absorption materials on restroom floors, and the continuous installation of pedestrian paths that are separate from bicycle paths were determined to be 'All Unsuitable' for all sections; therefore, it is necessary to improve the safety to prevent accidents. (6) In the 'Amenity' aspect, access roads, parking areas, hygiene facilities, convenience facilities, and waterside facilities for many sections were determined to be 'All Unsuitable'; therefore, it is necessary to conduct more concentrated hygiene management. (7) In the 'Accessibility(Mobility)' aspect, all items such as the installation of safe pedestrian paths in parking areas, and continuous pedestrian paths that are separate from bicycle paths were determined to be 'All Unsuitable' for all sections; therefore, it is necessary to improve the accessibility to provide safety and convenience. (8) In the 'Durability' aspect, access roads, parking areas, rest facilities, convenience facilities, fitness facilities, and waterside facilities were determined to be 'All Unsuitable' for many sections, therefore, it is necessary to improve sunken or damaged areas by inspecting facilities by section.

A Study on the Digital Drawing of Archaeological Relics Using Open-Source Software (오픈소스 소프트웨어를 활용한 고고 유물의 디지털 실측 연구)

  • LEE Hosun;AHN Hyoungki
    • Korean Journal of Heritage: History & Science
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    • v.57 no.1
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    • pp.82-108
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    • 2024
  • With the transition of archaeological recording method's transition from analog to digital, the 3D scanning technology has been actively adopted within the field. Research on the digital archaeological digital data gathered from 3D scanning and photogrammetry is continuously being conducted. However, due to cost and manpower issues, most buried cultural heritage organizations are hesitating to adopt such digital technology. This paper aims to present a digital recording method of relics utilizing open-source software and photogrammetry technology, which is believed to be the most efficient method among 3D scanning methods. The digital recording process of relics consists of three stages: acquiring a 3D model, creating a joining map with the edited 3D model, and creating an digital drawing. In order to enhance the accessibility, this method only utilizes open-source software throughout the entire process. The results of this study confirms that in terms of quantitative evaluation, the deviation of numerical measurement between the actual artifact and the 3D model was minimal. In addition, the results of quantitative quality analysis from the open-source software and the commercial software showed high similarity. However, the data processing time was overwhelmingly fast for commercial software, which is believed to be a result of high computational speed from the improved algorithm. In qualitative evaluation, some differences in mesh and texture quality occurred. In the 3D model generated by opensource software, following problems occurred: noise on the mesh surface, harsh surface of the mesh, and difficulty in confirming the production marks of relics and the expression of patterns. However, some of the open source software did generate the quality comparable to that of commercial software in quantitative and qualitative evaluations. Open-source software for editing 3D models was able to not only post-process, match, and merge the 3D model, but also scale adjustment, join surface production, and render image necessary for the actual measurement of relics. The final completed drawing was tracked by the CAD program, which is also an open-source software. In archaeological research, photogrammetry is very applicable to various processes, including excavation, writing reports, and research on numerical data from 3D models. With the breakthrough development of computer vision, the types of open-source software have been diversified and the performance has significantly improved. With the high accessibility to such digital technology, the acquisition of 3D model data in archaeology will be used as basic data for preservation and active research of cultural heritage.

An Analysis of the Locational Selection Factors of the Small- and Medium-sized Hospitals Using the AHP : Centered on the Spine and Joint Hospitals (AHP를 이용한 중·소 병원 입지선택요인 분석 : 척추·관절 병원중심으로)

  • Kim, Duck Ki;Shim, Gyo-Eon
    • The Journal of the Korea Contents Association
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    • v.18 no.5
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    • pp.191-214
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    • 2018
  • This research empirically analyzed the selection factors and the locational selection factors of the medical service facilities according to the gradual increase of the importance of the selection factors and the locational selection factors regarding the establishments of the small- and medium-sized hospitals according to the rapid changes of the socio-economic conditions. By analyzing the priority order according to the levels of the importance of each evaluation item factor through a research related to the selection factors and the locational selection factors of the small- and medium-sized hospitals and by drawing what the important factors that have the influences on the competitiveness of the pre-existent small- and medium-sized hospitals are through the classification of the real estate locational factors and the non-locational factors, the purpose lies in utilizing them as the basic data and materials for the opening strategies of the small- and medium-sized hospitals considering the special, locational characteristics according to the important factors of the selection factors of the small- and medium-sized hospitals, regarding the medical suppliers that have been preparing, for opening the new, small- and medium-sized hospitals. Based on the results of the preceding researches and the researches on the case examples, 28 evaluation factors were arrived at in terms of the level of the medical treatment, the medical services, the accessibilities of the hospitals, the conveniences of the hospitals, and the physical environment. And, regarding the 28 detailed evaluation factors that had been collected, through the interviews with the related experts, the 5 factors of the medical level, the medical service, the expertise of the hospital, the convenience of the hospital, and the physical environment were selected as the upper class evaluation factors. And, according to each upper class, a total of 28 low-part evaluation factors were selected. Regarding the optimal evaluation factors that were selected, the optimal locational factors were selected by carrying out an AHP questionnaire survey investigation with 200 medical experts as the subjects. Regarding the AHP analysis results, similarly with the case examples of the precedent researches, the levels of the importance appeared in the order of the medical level, the medical services, the accessibility of the hospital, the physical environment, and the convenience. And the factors that were related to the facilities of a hospital appeared low. The results of this research can be applied in providing the basis for the decision-makings regarding the selections of the locations of the small- and medium-sized hospitals in the future.

A basic research for evaluation of a Home Care Nursing Delivery System (가정간호 서비스 질 평가를 위한 도구개발연구)

  • Kim, Mo-Im;Cho, Won-Jung;Kim, Eui-Sook;Kim, Sung-Kyu;Chang, Soon-Bok;Ryu, Ho-Sihn
    • Journal of Home Health Care Nursing
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    • v.6
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    • pp.33-45
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    • 1999
  • The purpose of this study was to develop a basic framework and criteria for evaluation of quality care provided to patients with the attributes of disease in the home care nursing field, and to provide measurement tools for home health care in the future. The study design was a developmental study for evaluation of hospital-based HCN(home care nursing) in Korea. The study process was as follows: a home care nursing study team of College of Nursing. Yonsei University reviewed the nursing records of 47 patients who were enrolled at Yonsei University Medical Center Home Care Center in March, 1995. Twenty-five patients were insured at that time, were selected from 47 patients receiving home care service for study feasibility with six disease groups; Caesarean Section (C/S), simple nephrectomy, Liver cirrhosis(LC), chronic obstructive pulmonary disease(COPD), Lung cancer or cerebrovascular accident(CVA). In this study, the following items were selected : First step : Preliminary study 1. Criteria and items were selected on the basis of related literature on each disease area. 2. Items were identified by home care nurses. 3. A physician in charge reviewed the criteria and content of selected items. 4. Items were revised through preliminary study offered to both HCN patients and discharged patients from the home care center. Second step : Pretest 1. To verify the content of the items, a pretest was conducted with 18 patients of which there were three patients in each of the six selected disease groups. Third step : Test of reliability and validity of tools 1. Using the collected data from 25 patients with either cis, Simple nephrectomy, LC, COPD, Lung cancer, or CVA. the final items were revised through a panel discussion among experts in medical care who were researchers, doctors, or nurses. 2. Reliability and validity of the completed tool were verified with both inpatients and HCN patients in each of field for researches. The study results are as follows: 1. Standard for discharge with HCN referral The referral standard for home care, which included criteria for discharge with HCN referral and criteria leaving the hospital were established. These were developed through content analysis from the results of an open-ended questionnaire to related doctors concerning characteristic for discharge with HCN referral for each of the disease groups. The final criteria was decided by discussion among the researchers. 2. Instrument for measurement of health statusPatient health status was measured pre and post home care by direct observation and interview with an open-ended questionnaire which consisted of 61 items based on Gorden's nursing diagnosis classification. These included seven items on health knowledge and health management, eight items on nutrition and metabolism, three items on elimination, five items on activity and exercise, seven items on perception and cognition, three items on sleep and rest, three items on self-perception, three items on role and interpersonal relations, five items on sexuality and reproduction, five items on coping and stress, four items on value and religion, three items on family. and three items on facilities and environment. 3. Instrument for measurement of self-care The instrument for self-care measurement was classified with scales according to the attributes of the disease. Each scale measured understanding level and practice level by a Yes or No scale. Understanding level was measured by interview but practice level was measured by both observation and interview. Items for self-care measurement included 14 for patients with a CVA, five for women who had a cis, ten for patients with lung cancer, 12 for patients with COPD, five for patients with a simple nephrectomy, and 11 for patients with LC. 4. Record for follow-up management This included (1) OPD visit sheet, (2) ER visit form, (3) complications problem form, (4) readmission sheet. and (5) visit note for others medical centers which included visit date, reason for visit, patient name, caregivers, sex, age, time and cost required for visit, and traffic expenses, that is, there were open-end items that investigated OPD visits, emergency room visits, the problem and solution of complications, readmissions and visits to other medical institution to measure health problems and expenditures during the follow up period. 5. Instrument to measure patients satisfaction The satisfaction measurement instrument by Reisseer(1975) was referred to for the development of a tool to measure patient home care satisfaction. The instrument was an open-ended questionnaire which consisted of 11 domains; treatment, nursing care, information, time consumption, accessibility, rapidity, treatment skill, service relevance, attitude, satisfaction factors, dissatisfaction factors, overall satisfaction about nursing care, and others. In conclusion, Five evaluation instruments were developed for home care nursing. These were (1)standard for discharge with HCN referral. (2)instrument for measurement of health status, (3)instrument for measurement of self-care. (4)record for follow-up management, and (5)instrument to measure patient satisfaction. Also, the five instruments can be used to evaluate the effectiveness of the service to assure quality. Further research is needed to increase the reliability and validity of instrument through a community-based HCN evaluation.

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