• Title/Summary/Keyword: absorption spectra

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Action Spectra of Apoptosis Induction and Reproductive Cell Death in L5178Y cells in UV-B Region

  • Mizuho Aoki;Yoshiya Furusawa;Higashi, Sho-ichi;Masakatsu Watanabe
    • Journal of Photoscience
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    • v.9 no.2
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    • pp.454-456
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    • 2002
  • It is important to determine the action spectrum of UV-B radiation contained in the sunlight to estimate the risk of skin cancer. We have investigated action spectra for induction of apoptosis and reproductive cell death in L5178Y cells using the Okazaki Large Spectrograph at NIBB. L5178Y cells were exposed to light at different wavelengths in UV-B or UV-A region. Frequencies of apoptosis induction and reproductive cell death were determined by counting cells with chromatin condensation, and by the colony formation assay, respectively. The measured sensitivity spectra for the two end-points were in very good agreement. Sensitivity decreased steeply with increase of wavelength in UV-B region and remains nearly constant in UV-A region. The action spectra were also slightly steeper than that for the minimum erythematic dose (MED), but very similar to the light absorption spectrum of DNA in UV-B region. On the other hand, the spectra for both endpoints were similar to MED spectrum but not DNA spectrum in the UV-A region. Also different time-course and morphological difference of apoptosis were found between UV-B (long time, fragmentation) and UV-A (short time, shrinkage) region. These results suggest that DNA damage induced by UV-B light triggers apoptosis and reproductive cell death, but other damaged targets (membrane, protein and so on) trigger these effects in UV-A region.

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A Study on the Optical Properties by Material of Soft Contact Lens (소프트콘택트렌즈의 재료에 따른 광학적 특성에 관한 연구)

  • Son, Byeong-Ho;Jeong, Ju-Hyun
    • Journal of Korean Ophthalmic Optics Society
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    • v.14 no.2
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    • pp.27-30
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    • 2009
  • Purpose: Transmittance at UV-visible spectra range was measured to confirm the effect of wet/dry state of commercially available SCL on human eyes. Method: Commercially available five SCLs fabricated by different materials were selected to measure optical properties according to humidity. Results: A relatively high transmittance above about 80% in the visible spectra range of the SCLs were observed. In the UV spectra range, the wet samples showed higher transmittance than those dried except an unusual case. Conclusions: All the samples having a high optical properties in the visible spectra range exhibit a high transmittance in the UV region. Further research will be needed to develop new materials having a sharp absorption edge in the UV spectra range.

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The IGRINS Spectra of Late-Type Stars

  • Park, Sunkyung;Lee, Jeong-Eun;Kang, Wonseok;Lee, Sang-Gak;Chun, Moo-Young;Kim, Kang-Min;Yuk, In-Soo;Jeong, Ueejeong;Jaffe, Daniel T.
    • The Bulletin of The Korean Astronomical Society
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    • v.40 no.2
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    • pp.58.2-58.2
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    • 2015
  • We present a library of high spectral resolution (R ~ 40,000) and high signal-to-noise ratio (S/N ~ 200) near-infrared spectra of ~50 late-type stars. The spectra of late-type stars were obtained with Immersion GRating INfrared Spectrograph (IGRINS) covering the full H and K band. The stars are mainly from MK standard stars which have well-defined spectral types and luminosity classes and cover wide ranges of effective temperatures and surface gravities. The spectra are corrected for telluric absorption lines and absolutely flux calibrated using the Two Micron All Sky Survey (2MASS) photometry. In this work, we present the preliminary results of spectroscopic diagnostics for stellar physical parameters. Our ultimate goal is to provide a library of near-infrared spectra of standard stars, which covers all spectral types and luminosity classes, with a high spectral resolution and high signal-to-noise ratio.

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Characterization of Methanol-Water and Acetonitrile-Water Mixtures Using Iterative Target Transform Factor Analysis on Near Infrared Absorption Spectra (근적외선흡광스픽트럼에 대한 반복목표변환인자분석에 의한 메탄올-물 혼합액 및 아세토니트릴 -물 혼합액의 특성 확인)

  • 박영주;조정환
    • YAKHAK HOEJI
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    • v.48 no.1
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    • pp.6-12
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    • 2004
  • Near-infrared spectra of methanol-water mixtures and acetonitrile-water mixtures were acquired to find interactions between solvents widely used for reverse-phase liquid chromatography. Mixtures were prepared to give a series of increasing mole fractions of methanol or acetonitrile in water. Data matrices of acquired spectra were analyzed to determine the proper number of principal components of each mixture system using Malinowski's factor indicator function. Initial guess of score matrix and loading matrix were calculated by nonlinear iterative partial least squares (NIPALS) algorithm for faster computation. Iterative target transform factor analysis (ITTFA) was applied to convert the initial estimation of score matrix to true concentration profile and loading matrix to pure spectra of pure components of the mixtures. In case of methanol-water the number of principal components was found to be 4 and those initial guess of factors were converted to the pure spectra of water methanol and two kinds of complexes. In case of acetonitrile-water the number of pure components of the mixtures was found to be 3 and the pure spectrum of acetonitrile-water complex was found. The nonlinear characteristics of concentration profiles of complexes in the solvent mixtures may give a good criteria in understanding their elution characteristics in reverse-phase liquid chromatogrsphy.

Ab Initio Study of Vibrational Spectra of p-tert-Butylcalix[4]aryl Ester Complexed with Alkali Metal Cation (알칼리금속 양이온과 착물을 형성한 캘릭스[4]아릴에스터의 진동스펙트럼에 대한 순수양자역학적 연구)

  • Choe, Jong-In;Kim, Gwang-Ho
    • Journal of the Korean Chemical Society
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    • v.50 no.1
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    • pp.7-13
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    • 2006
  • infrared(IR) absorption spectra were calculated for the ethyl ester of p-tert-butylcalix[4]arene (1) in the cone conformer and its alkali-metal-ion complex. The vibrational spectra were obtained by restricted Hartree-Fock (RHF) calculations with the 6-31G basis set. The characteristic vibrational frequencies of various C-O and C=O stretching motions of the complexes show that the structure of 1+K+ complex is almost of C4v symmetry compared to 1+Na+ (C2v) analogue. The theoretical results for the host molecule 1 and complex (1+Na+) were compared with the experimental results, and the calculated vibrational frequencies agree well with the features of the experimental spectra.

DOUBLE STARS AS TRACERS OF TINY STRUCTURES IN THE INTERSTELLAR MEDIUM

  • MORABBI, SOMAYEH;MIRTORABI, MOHAMMAD TAGHI
    • Publications of The Korean Astronomical Society
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    • v.30 no.2
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    • pp.89-91
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    • 2015
  • Observed spectra of stars around the Sun have indicated that the Sun is located in a gas cavity, extending to 100pc. This gas cavity is called the "Local Bubble". The density of the interstellar medium (ISM) in the local bubble is about one tenth that of the average for the ISM in the Milky Way. Furthermore, some structures such as gas planes and strings in the local bubble are probably the result of supernovae. These, due to their low temperatures, can not be observed in the visible and infrared. The only way to do so is to measure the spectra of nearby stars so that the light of stars passing through the local bubble is absorbed by existing gas and the resulting spectral lines from absorption can be measured. In this study, we use binary stars to trace the local bubble structures through lines such as the Na I Doublet. First, we determined the observed spectral lines of stars by HARPS and FEROS echelle spectrographs. Then, we made synthetic spectra with the ATLAS9 code. Finally, the difference between the observational and synthetic spectra confirms the existence of the Na I Doublet in the local ISM.

Far ultraviolet observations of diffuse, monoenergetic, and broadband auroras

  • Lee, Jun-Chan;Min, Kyoung-Wook;Lee, Chi-Na
    • The Bulletin of The Korean Astronomical Society
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    • v.37 no.2
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    • pp.134.2-134.2
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    • 2012
  • Discrete auroras, with unique shapes embedded in diffuse auroras, are generally associated with precipitating electrons that originate from the plasma sheet and are accelerated on the way as they travel to polar regions along the field lines. Two acceleration mechanisms have been proposed: quasi-static electric fields and dispersive Alfven waves, which are believed to yield monoenergetic peaks and broadband features in the particle spectra, respectively. Hence, it should be interesting to see how the two different mechanisms, through their characteristic spectra of the accelerated electrons, produce distinct auroral images and spectra, especially in the far ultraviolet (FUV) wavelengths as the long and short Lyman-Birge-Hopfield (LBH) bands exist as well as the strong absorption band of molecular oxygen in the FUV band. In fact, we have previously shown, using the simultaneous observations of precipitating electrons and the corresponding FUV spectra, that the discrete auroras associated with inverted-V events have a stronger relative intensity of the long LBH to the short LBH compared to diffuse auroras, especially when the peak energy is above a few keV. In this paper, we would like to focus on the differences in the FUV images and spectra between the two discrete auroras of the monoenergetic and broadband cases, again based on the study using the dataset of simultaneous observations of particles and FUV spectral images.

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Crystal growth and optical absorption of $Mg_{0.16}Zn_{0.84}Te:Co $ single crystal ($Mg_{0.16}Zn_{0.84}Te:Co $단결정 성장과 광흡수 특성)

  • 정상조
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.7 no.4
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    • pp.548-554
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    • 1997
  • The single crystal of $Mg_{0.16}Zn_{0.84}$Te:Co(Co:0.01 mole%) was grown by vertical Bridgman method. The crystal structure of $Mg_{0.16}Zn$_{0.84}$Te:Co and optical absorption properties of this compound were studied. The grown single crystal has a cubic structure and a lattice constant a=6.1422 $\AA$ were determined by X-ray diffraction. As a result of the optical absorption spectra of $Mg_{0.16}Zn_{0.84}$Te:Co, the intracenter transitions due to $Co^{2+}$ ions were detected for $A-band:^4A_2(^4F){\to}^4T_2(^4F),\; B-band:^4A_2(^4F){\to}^4T_1(^4F), C- band:^4A_2(^4F){\to}^4T_1(^4P)$.The charge transfer transition near the absorption edge was observed in the wavelength range of 550 to 770 nm. According to the crystal field theory, the crystal field parameter(Dq) and the Racah parameter(B) were determined.

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Determination of Sesame oil Adulterated with other Vegetable oils by Spectrophotometric Method (자외선 흡수특성을 이용한 참기름의 이종기름 혼입판별에 관하여)

  • 이영근
    • Journal of Food Hygiene and Safety
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    • v.8 no.3
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    • pp.151-155
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    • 1993
  • Since there have been no method which can applicable to the screening of commercial sesame oil adulterated with other vegetable oils, the present investigation was carried out particularily focusing on the the pattern of IN absorption of sesame oil and other vegetable oils. For this, a variety of oil samples prepared by the conventional method from sesame seeds, perilla seeds, com, soybean, and rice bran were analyzed by IN spectrophotometer. IN spectra of sesame oil and oil of unheated sesame seeds showed absorption peaks at 215, 230 and 290 nm. While UV spectra of com oil, perilla oil and soybean oil all showed absorption peaks at 215, 230 and 280 nm, that of rice bran oill showed peaks at 215, 290 320 nm. When sesame oil was mixed with com oil, perilla oil or soybean oil, respectively, from which the absorbance of peaks at 290 nm were lower than pure sesame oil. The peak at 320 nm which was typical absorption peak of rice bran oil was still observed in the spectnun of mixture of sesame oil with rice bran oil.

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UV-cut Lens Manufacture using Optical Absorption Edge Control (광흡수단을 제어를 이용한 UV 차단렌즈 개발)

  • Kim, Yong Geun;Park, Dong Hwa;Sung, Jung Sub
    • Journal of Korean Ophthalmic Optics Society
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    • v.7 no.1
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    • pp.29-34
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    • 2002
  • Because do dilution (5%) in Venzotriazol distilled water and is kept UV interception departure woe which make to time each 1, 2, 5, 15, 20, 30 minutes by dip method, produced sample ore of UV interception lens. Spectrum of transmittance for each sample are measured in 320~450nm sacred grounds. The result optical absorption edge is each 403, 408, 414, 419nm regions, and absorption edge appeared in each band of 3.07, 3.04, 2.99, 2.96 eV. UV-cut lens departure solution make 2-Hydroxy-4-n-octoxyhenzophenones of 5%, 10% because solvent methanol and UV-cut lens made by Spray pyrolysis method. Transmission edge of each lens that do spray to same warelength showed sameinwavelength spectra. Being storehouse absorption edgethat increase 385, 398 and 417nm region of when do spray for 2, 4 and 6 minutes, band is accomplishing each 3.22, 3.11, 2.97 eV.

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