• Preventive Nutrition and Food Science
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• 제22권2호
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• pp.67-80
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• 2017

Cholesterol is a vital component of the human body. It stabilizes cell membranes and is the precursor of bile acids, vitamin D and steroid hormones. However, cholesterol accumulation in the bloodstream (hypercholesterolemia) can cause atherosclerotic plaques within artery walls, leading to heart attacks and strokes. The efficiency of cholesterol absorption in the small intestine is of great interest because human and animal studies have linked cholesterol absorption with plasma concentration of total and low density lipoprotein cholesterol. Cholesterol absorption is highly regulated and influenced by particular compounds in the food supply. Therefore, it is desirable to learn more about natural food components that inhibit cholesterol absorption so that food ingredients and dietary supplements can be developed for consumers who wish to manage their plasma cholesterol levels by non-pharmacological means. Food components thus far identified as inhibitors of cholesterol absorption include phytosterols, soluble fibers, phospholipids, and stearic acid.

• Nutrition Research and Practice
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• 제3권1호
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• pp.9-14
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• 2009

Aloe products are one of the top selling health-functional foods in Korea, however the adequate level of intake to achieve desirable effects are not well understood. The objective of this study was to determine the intestinal uptake and metabolism of physiologically active aloe components using in vitro intestinal absorption model. The Caco-2 cell monolayer and the everted gut sac were incubated with $5-50{\mu}M$ of aloin, aloe-emodin, and aloesin. The basolateral appearance of test compounds and their glucuronosyl or sulfated forms were quantified using HPLC. The % absorption of aloin, aloe-emodin, and aloesin was ranged from 5.51% to 6.60%, 6.60% to 11.32%, and 7.61% to 13.64%, respectively. Up to 18.15%, 18.18%, and 38.86% of aloin, aloe-emodin, and aloesin, respectively, was absorbed as glucuronidated or sulfated form. These results suggest that a significant amount is transformed during absorption. The absorption rate of test compounds except aloesin was similar in two models; more aloesin was absorbed in the everted gut sac than in the Caco-2 monolayer. These results provide information to establish adequate intake level of aloe supplements to maintain effective plasma level.

• 한국식품영양과학회지
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• 제23권3호
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• pp.396-401
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• 1994

The effect of vitamin B12 status and pectin feeding on vitamin B12 absorption in rats were studied. Rats in low, medium, and high vitamin B12 status were fed either fiber-free or 10% pectin diet and absorption of a single oral dose of vitamin B12 status of rats was verified by urinary methylmalonic acid and liver vitamin B12 measurements. Absorption of vitamin B12 tended to increase as the rats became deficient in vitamin B12 , although the difference was not significant . Pectin inhibited absorption of vitamin B12 regardless of the vitamin B12 status of the rats. The results demonstrated that inhibition of vitamin B12 absorption by pectin would be a possible mechanism for the impairment of vitamin B12 status due to chronic pectin feeding.

• 한국식품영양학회지
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• 제15권1호
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• pp.7-11
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• 2002

학령전 아동의 zinc absorption, excretion, balance에 대하여 연구한 결과는 다음과 같다. Zinc absorption은 남자아동의 경우 29.7%, 여자아동의 경우 23.3%, 평균 25.8%로 나타났다. 대상자기 zinc absorption은 zinc intake와는 상관성이 나타나지 않았으며, zinc balance와 유의적 상관(p<0.01)을 보였다. Zinc balance는 남자아동의 경우 평균 3.25mg/day , 여자아동의 경우 1.78mg/day , 평균 2.34mg/day 로 1명의 대상자를 제외하고 모두 positive balance를 보였다. Fecal zinc loss는 평균 6.31mg/day, urinary ziilc loss는 평균 0.16mg/day 였다. zinc intake는 focal zinc loss및 zinc balance에 유의적 영향을 미치는 것으로 나타났으며(p<0.01), urinary zinc는 intake의 영향을 받지 않았다. 우리나라 학령전 아동의 zinc absorption 및 balance 는 양호한 것으로 나타났다. 그러나 이 방면의 연구 자료가 많지 않아, 성장기 아동을 대상으로 한 zinc nutrition에 대한 구체적이고 지속적인 연구가 필요하다.

• Journal of Nutrition and Health
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• 제23권6호
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• pp.427-433
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• 1990

식이내 상이한 수준의 펙틴이 비타민 B$_{12}$의 흡수에 미치는 영향을 조사하였다. 무섬유식이를 섭취해온 흰쥐를 비타민 B$_{12}$흡수 정도를 측정하려는 전날, 무섬유, 2% 펙틴, 5% 펙틴, 그리고 10% 펙틴 식이군으로 나누었다. 다음날, 동물이 각기 배정된 식이를 섭취하는 동안, 57-Co-비타민 B$_{12}$를 구강으로 섭취시켜 그 흡수 정도를 측정하였다. 무섬유 식이와 비교하여 볼 때, 5%와 10% 펙틴 식이는 비타민 B$_{12}$의 흡수를 유의적으로 감소시켰다. 펙틴의 섭취량은 비타민 B$_{12}$의 흡수와 역상관 관계를 나타내었다.

• 한국식품영양과학회:학술대회논문집
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• 한국식품영양과학회 2001년도 International Symposium on Food,Nutrition and Health for 21st Century
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• pp.74-87
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• 2001

Evidence shows that the serum level of cholesterol (CH) is decreased with increasing green tea (GT) consumption. This presentation summarizes our recent findings on the effect of GT extract on intestinal absorption of [$^{14}C$-labeled CH and phosphatidylcholine (PC). Ovariectomized (OX) adult rats were infused intraduodenally with lipid emulsions containing radiolabeled lipids [$^{14}C$-CH or $^{14}C$-phosphatidylcholine (PC)] in the presence of GT extract or catechins to determine the rates and amounts of CH absorption and the intestinal hydrolysis and lymphatic output of PC. During lipid infusion, lymph was collected hourly for 8 h. The lymphatic absorption of $^14C$-CH was drastically lowered by infusion of GT extract at two dosage levels (GTl =5.4 mg catechins/h and GT2 = 15.1mg catechins/h). The cumulative lymphatic absorptions of $^{14}C$-CH in rats infused with GT1 and GT2 were 20.7$\pm$4.3 and $4.8{\pm}4.1{\%}$ dose, respectively, whereas the absorption of $^{14}C$-CH in rats infused with no GT extract (GT0) was $36.3{\pm}1.1{\%}$ dose. GT extracts also significantly lowered the absorption of-tocopherol (TP) in a dose dependent manner ($29.6{\pm}4.9{\%}$ dose in GT0, $20.8{\pm}5.8{\%}$ dose in GTl, and $7.9{\pm}5.4{\%}$ dose in GT2 groups). Both (+)-catechin and EGCG significantly lowered the lymphatic outputs of $^{14}C$-radioactivity after intraduodenal $^{14}C$-PC infusion. A significantly higher amount of $^{14}C$-PC remained unhydrolyzed in the intestinal lumen of the EGCG rats ($22.8{\%}$) compared with the (+)-catechin ($15.8\%$) and control groups ($11.9\%$). GT extracts, (+)-catechin, and EGCG significantly reduced the absorption of TP. The inhibitory effect of GT extract and catechins on lipid absorption may be mediated in part through the inhibition of pancreatic PLAz. The findings provide the first direct evidence that green tea and catechins have a profound inhibitory effect on the intestinal absorption of CH in OX rats. Results suggest that green tea and catechins may be used as a dietary or pharmacological means of lowering cholesterol absorption.

• 한국식품영양과학회:학술대회논문집
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• 한국식품영양과학회 2004년도 Annual Meeting and International Symposium
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• pp.47-52
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• 2004

This study was conducted to determine the effects of egg phospholipids [(Phosphatidylcholine (PC) and sphingomyelin (SM)] on intestinal absorption of cholesterol and other lipids. Each rat with Iymph cannula was infused via a duodenal catheter at 3.0 mL/h for 8 h with a lipid emulsion containing triolein, cholesterol and PC in 24 mL PBS. The PC in the lipid emulsion was egg PC (EPC), hydrogenated egg PC (HPC), or soy PC (SPC). The EPC in the lipid emulsion markedly lowered the Iymphatic absorption of cholesterol, compared with SPC and a lipid emulsion containing no PC. The HPC further lowered the absorption of cholesterol. The phospholipid output was not affected by the source of PC infused. The total Iymphatic output of oleic acid (18: 1), the major fatty acid infused in the form of triolein, did not differ among the NPC, SPC and EPC groups, but was significantly lower in the HPC group. The findings provide the first evidence that EPC markedly lowers the Iymphatic absorption of cholesterol under in vivo conditions. The inhibitory effect of EPC appears to be due to the higher degree of saturation of its acyl groups relative to SPC, suggesting that the intestinal absorption of egg cholesterol may be reduced by the presence of PC in e99 yolk. Experiment 2 was designed to determine whether egg SM, structurally similar to PC, also inhibits the Iymphatic absorption of cholesterol. Egg SM lowered the Lymphatic absorption of cholesterol in a dose dependent manner. Likewise, SM lowered the Iymphatic absorption of oleic acid, whereas it had no effect on retinol absorption. SM at a high dose lowered the Iymphatic outputs of both PC and SM, whereas there was no such effect at a lower dose. These results also indicate that luminal egg SM has an inhibitory effect on the intestinal absorption of cholesterol and other lipids of relatively high hydrophobicity.

• Nutrition Research and Practice
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• 제2권1호
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• pp.3-7
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• 2008

This study was carried out to investigate the efficacy of sn-2 palmitic acid-fortified vegetable oil (Sn2PA) on calcium absorption and to confirm the synergistic effects of fructooligosaccharide on calcium absorption. Male SD rats were fed 6 kinds of casein based diets containing vegetable oil (control), sn-2 palmitic acid-fortified vegetable oil (Sn2PA) and Sn2PA with fructooligosaccharide(Sn2PAFO) in two levels of calcium (normal 0.5% and high 1.0%) for 3 weeks. Total lipids, cholesterol, triglyceride and calcium in blood were measured. Feces were collected using cages for 4 days. Serum concentrations of total lipids and calcium were not significantly different among groups. However, serum triglyceride was significantly decreased by fructooligosaccharide supplementation regardless of dietary calcium level. The lipid absorption was not significantly different among experimental groups. Calcium absorption was significantly higher in Sn2PAFO group than other groups. Calcium solubility of intestine was increased by sn-2 palmitic acid supplementation. These results suggest that sn-2 palmitic acid and fructooligosaccharide supplementation could be beneficial for baby foods including infant formula, with regard to increasing absorption of calcium by more soluble calcium in the small intestinal content.

• International Journal of Human Ecology
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• 제4권1호
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• pp.35-44
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• 2003

This study investigated the effects of dietary phyate reduction on the apparent absorption and biochemical parameters of iron status in young Korean women. Fourteen healthy, young women consumed low and high phytate diets for ten days of each experimental period. Duplicate diet samples, a fasting blood sample on day 9, and complete fecal samples for five consecutive days starting from day 5 of each diet period were collected. The iron content of diet and fecal samples were analyzed to calculate apparent absorption. Serum samples were analyzed for iron, ferritin, transferrin receptor and TIBC; transferrin saturation was also calculated. The apparent absorption of iron tended to increase in the low phytate period (32.51%) compared to the high phytate period (17.91%), but the difference was not significant (p=0.06). Serum ferritin decreased and serum transferrin receptor increased significantly during the low phytate diet although the mean values were within the normal range. Serum iron and transferrin saturation did not change significantly. In conclusion, the results indicated that reducing dietary phytate for ten days negatively affected iron nutritional parameters, but it moderately and positively affected apparent iron absorption in young Korean women. Further research on the long-term effects of a low phytate diet with an adequate iron content for vows Korean women is necessary.

• Nutritional Sciences
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• 제7권3호
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• pp.151-157
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• 2004

The aim of this study was to investigate the effects of chicory fructan fiber supplementation on bone mineral density, apparent absorption of minerals and serum parameters related to bone turnover in postmenopausal women. Twenty-six healthy Korean postmenopausal women participated in the study. 1be participants were randomly divided into two groups in a double-blind parallel design and took one of the supplements for 3 months; either a placebo of 8g maltodextrins/sucrose mixture (control group) or 8g chicory fructan fiber (fructan group). During the 3-month experimental period no differences were found in bone mineral density (BMD) between the two groups. Apparent calcium absorption significantly increased by 42% in the fructan group, while that of the control group decreased by 29% as compared to the values at baseline. Urinary calcium excretion was not significantly different between the group;;. After 3 months, the level of serum alkaline phosphatase (ALP) was significantly lower in the fructan group than in the control group and deoxypyridinolin showed a trend toward a slight reduction. In conclusion, intake of chicory fructan fiber with a regular increases apparent calcium absorption in postmenopausal women.