• 농업과학연구
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• 제44권1호
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• pp.67-76
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• 2017

Fermented Rhus verniciflua (FRV) as feed additives act as excellent anti-diarrheal drugs as they increase the intestinal absorption rate therefore being indirectly associated with enhancing growth performance and increasing digestibility in livestock. A total of 80 weaned pigs with an average initial body weight (BW) of $6.82{\pm}1.11kg$ were used to evaluate a diet supplemented with FRV meal in a 6 week feeding trial with two dietary treatments [CON - basal diet; TRT - CON + 0.2% FRV] on growth performance, apparent total tract digestibility (ATTD), blood profiles, fecal microflora, and fecal score. Our results showed that the dietary supplementation of FRV improved (p < 0.05) average daily gain and gain : feed (G : F) ratio during days 15 - 42 and the overall experiment period and also increased the ATTD of dry matter (p < 0.05) at days 14 and 42. On the contrary, there was no effect (p > 0.05) on average daily feed intake, ATTD of nitrogen and energy, and blood profiles during the entire experiment. Moreover, dietary inclusion of FRV significantly increased fecal Lactobacillus (p < 0.05) counts and reduced the diarrhea during days 22 - 42. Thus, the results suggest that FRV can be used as a potential additive to improve growth performance and dry matter and to reduce diarrhea while having beneficial effects on fecal microflora in weanling pigs.

• Structural Engineering and Mechanics
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• 제77권3호
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• pp.329-342
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• 2021

In this paper, the effect of different steel bar configurations on the quasi-static punching and impact response of concrete slabs was studied. A total of forty RC square slab specimens were cast in two groups of concrete strengths of 40 and 63 MPa. In each group of twenty specimens, ten specimens were reinforced at the back face (singly reinforced), and the remaining specimens were reinforced on both faces of the slab (doubly reinforced). Two rebar spacing of 25 and 100 mm, with constant reinforcement ratio and effective depth, were used in both singly and doubly reinforced slab specimens. The specimens were tested against the normal impact of cylindrical projectiles of hemispherical nose shape. Slabs were also quasi-statically tested in punching using the same projectile, which was employed for the impact testing. The experimental response illustrates that 25 mm spaced rebars are effective in (i) decreasing the local damage and overall penetration depth, (ii) increasing the absorption of impact energy, and (iii) enhancing the ballistic limit of RC slabs. The ballistic limit was predicted using the quasi-static punching test results of slab specimens showing a strong correlation between the dynamic perforation energy and the energy required for quasi-static perforation of slabs.

• Journal of Ginseng Research
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• 제46권2호
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• pp.248-254
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• 2022

Background: Zinc homeostasis is essential for human health and is regulated by several zinc transporters including ZIP and ZnT. ZIP4 is expressed in the small intestine and is important for zinc absorption from the diet. We investigated in the present study the effects of Panax ginseng (P. ginseng) extract on modulating Zip4 expression and cellular zinc levels in mouse Hepa cells. Methods: Hepa cells were transfected with a luciferase reporter plasmid that contains metal-responsive elements, incubated with P. ginseng extract, and luciferase activity was measured. Using ⁶⁵ZnCl₂, zinc uptake in P. ginseng-treated cells was measured. The expression of Zip4 mRNA and protein in Hepa cells was also investigated. Finally, using a luciferase reporter assay system, the effects of several ginsenosides were monitored. Results: The luciferase activity in cells incubated with P. ginseng extract was significantly higher than that of control cells cultured in normal medium. Hepa cells treated with P. ginseng extract exhibited higher zinc uptake. P. ginseng extract induced Zip4 mRNA expression, which resulted in an enhancement of Zip4 protein expression. Furthermore, some ginsenosides, such as ginsenoside Rc and Re, enhanced luciferase activity driven by intracellular zinc levels. Conclusion: P. ginseng extract induced Zip4 expression at the mRNA and protein level and resulted in higher zinc uptake in Hepa cells. Some ginsenosides facilitated zinc influx. On the basis of these results, we suggest a novel effect of P. ginseng on Zip4-mediated zinc influx, which may provide a new strategy for preventing zinc deficiency.

• Fisheries and Aquatic Sciences
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• 제26권6호
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• pp.406-422
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• 2023

Sarcopenia is an age-related, progressive skeletal muscle disorder involving the loss of muscle mass and strength. Previous studies have shown that γ-aminobutyric acid (GABA) from fermented oysters aids in regulatory T cells (Tregs) cell expansion and function by enhancing autophagy, and concomitantly mediate muscle regeneration by modulating muscle inflammation and satellite cell function. The fermentation process of oysters not only increases the GABA content but also enhances the content of branched amino acids and free amino acids that aid the level of protein absorption and muscle strength, mass, and repair. In this study, the effect of GABA-enriched fermented sarco oyster extract (FSO) on reduced muscle mass and functions via Treg modulation and enhanced autophagy in aged mice was investigated. Results showed that FSO enhanced the expression of autophagy markers (autophagy-related gene 5 [ATG5] and GABA receptor-associated protein [GABARAP]), forkhead box protein 3 (FoxP3) expression, and levels of anti-inflammatory cytokines (interleukin [IL]-10 and transforming growth factor [TGF]-β) secreted by Tregs while reducing pro-inflammatory cytokine levels (IL-17A and interferon [IFN]-γ). Furthermore, FSO increased the expression of IL-33 and its receptor IL-1 receptor-like 1 (ST2); well-known signaling pathways that increase amphiregulin (Areg) secretion and expression of myogenesis markers (myogenic factor 5, myoblast determination protein 1, and myogenin). Muscle mass and function were also enhanced via FSO. Overall, the current study suggests that FSO increased autophagy, which enhanced Treg accumulation and function, decreased muscle inflammation, and increased satellite cell function for muscle regeneration and therefore could decrease the loss of muscle mass and function with aging.

• 대한화학회지
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• 제48권1호
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• pp.46-52
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• 2004

층상구조를 갖는 망간산화물에서 결정성과 구조적 안정도 간의 관계에 대해 조사하였다. 좋은 결정성을 갖는 망간산화물은 고상합성법-이온교환법을 이용하여 합성하였으며, 나노결정 망간산화물은 실온에서의 Chimie-Douce 반응을 통해 얻어졌다. 마이크로 라만 분광과 X선 흡수분광 결과는 결정성에 상관없이 이들 화합물에 존재하는 망간이온이 공통적으로 층상구조의 팔면체 자리에 안정화되어 있음을 보여준다. 미분전하용량 분석 결과는 나노결정 화합물의 층상구조가 전기화학적 충방전 과정 동안 안정하다는 사실을 보여주며, 이와는 대조적으로 좋은 결정성을 갖는 층상구조 화합물의 경우 현저한 구조변화를 겪는다는 사실을 보여준다. 마이크로 라만 분광 결과는 이러한 구조전이가 층상 구조로부터 스핀넬 타입 구조로의 변화에 해당함을 보여준다. 위 실험 결과로부터 나노결정성이 층상구조의 안정도를 향상시킨다는 결론을 얻을 수 있었다.

• 콘크리트학회지
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• 제11권2호
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• pp.209-220
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• 1999

터널의 숏크리트 라이닝이나 포장 콘크리트 보강용으로 요접철망(wire mesh)을 대신해서 강섬유가 사용되고 있다. 본 연구에서는 강섬유 보강으로 인한 인성평가 대상 구조물을 slab panel 구조($60{\times}60{\times}10$cm)로 하고, 강섬유 혼입률은 콘크리트 용적의 0.5% ~ 2%로 다양화하였다. 이 때 사용한 강섬유는 Dramix ZC type으로 직경은 0.8mm, 길이는 60mm이다. 강섬유 효과의 상대평가를 위한 용접철망(wire mesh)보강은 상면, 하면, 상하면 보강으로 하였다. 이들 실험 결과를 각국의 인성 평가 방법으로 비교 검토한 결과 슬래브(slab) 시험체 적용을 위한 EFNARC의 방법은 25mm의 처짐까지 측정하는 것이 너무 큰 것으로 평가되었고, 보의 휨인성 평가법을 적용하여 검토한 결과에서는 Johnston(II)방법에 의한 $I_{5.5}$ 가장 적절하였으며, JCI-SF4방법에서 지간의 1/150까지 측정하는 것은 너무 작았다. 또한 강섬유로 용접철망(wire mesh)을 효과적으로 대치할 수 있음을 알 수 있었고, 인성효과에 유용한 강섬유 혼입량은 0.5% ~ 1%범위에 있는 것으로 나타났다.

• BMB Reports
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• 제51권9호
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• pp.474-479
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• 2018

Cisplatin is one of the most effective chemotherapeutic drugs used in the treatment of HCC, but many patients will ultimately relapse with cisplatin-resistant disease. Used in combination with cisplatin, resveratrol has synergistic effect of increasing chemosensitivity of cisplatin in various cancer cells. However, the mechanisms of resveratrol enhancing cisplatin-induced toxicity have not been well characterized. Our study showed that resveratrol enhances cisplatin toxicity in human hepatoma cells via an apoptosis-dependent mechanism. Further studies reveal that resveratrol decreases the absorption of glutamine and glutathione content by reducing the expression of glutamine transporter ASCT2. Flow cytometric analyses demonstrate that resveratrol and cisplatin combined treatment leads to a significant increase in ROS production compared to resveratrol or cisplatin treated hepatoma cells alone. Phosphorylated H2AX (${\gamma}H2AX$) foci assay demonstrate that both resveratrol and cisplatin treatment result in a significant increase of ${\gamma}H2AX$ foci in hepatoma cells, and the resveratrol and cisplatin combined treatment results in much more ${\gamma}H2AX$ foci formation than either resveratrol or cisplatin treatment alone. Furthermore, our studies show that over-expression of ASCT2 can attenuate cisplatin-induced ROS production, ${\gamma}H2AX$ foci formation and apoptosis in human hepatoma cells. Collectively, our studies suggest resveratrol may sensitize human hepatoma cells to cisplatin chemotherapy via gluta${\gamma}H2AX$mine metabolism inhibition.

• Journal of Forest and Environmental Science
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• 제36권3호
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• pp.225-232
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• 2020

Chemical modification of wood is an effective method to enhance the biological durability of wood with no toxic effect on the environment. In this study, wood of Triplochiton scleroxylon was modified using acetylation techniques. A total of one hundred wood blocks, (each 20×20×60 mm) obtained from a 22-year old T. scleroxylon tree were conditioned and acetylated at 120℃ in a bioreactor containing acetic anhydride for 60, 120, 180, 240 and 300 minutes. The percentage weight gain of acetylated wood was determined. The untreated (control) and treated blocks were exposed to Pleurotus ostreatus (white rot fungus) and Fibroporia vaillanti (brown rot fungus) after which moisture content (MC) and weight loss (WL) was monitored for 16 weeks. Data were analysed using descriptive and inferential statistics at p<0.05 level of significance. The percentage weight gain of acetylated wood samples increased with time from 10.4% (60 minutes) to 22.7% (300 minutes). MC of untreated blocks inoculated with Pleurotus ostreatus was significantly higher than those of Fibroporia vaillantii after 16 weeks exposure. There was no significant difference in the MC of the of the acetylated samples for the two fungi after 300 minutes reaction time. The WL of untreated blocks inoculated with Fibroporia vaillantii was higher than those of Pleurotus ostreatus, however, the two fungi showed no significant difference in the WL for the acetylated samples after 16 weeks exposure. Acetylation prevents moisture absorption and inhibition of fungi growth in acetylated wood compared to untreated wood, thereby enhancing the durability of Triplochiton scleroxylon.

• 한국연초학회지
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• 제2권1호
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• pp.37-52
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• 1980

신개간지 토양에 대한 인산시용 수준별 석회, 퇴비증시, 퇴비와 붕소의 혼합시용에 따른 비옥도 증진의 효과가 연초의 수량 및 품질에 미치는 영향을 2년간에 걸쳐 시험한 결과를요약하면 다음과 같다. 1. 개량제 처리의 효과는 1차년도에서 보다 2차년도에 담배 생육과 엽면적지수와 단위엽 면적중을 월등하게 증가시켰다. 3. 개량제의 시용으로 초년도의 pH, 치환성염기등이 숙전과 같은 수준으로 개선되었으며, 처리별 비옥도 증진효과는 퇴비증시구가 컸다. 3. 2차년도 시험결과에서 수량은 대조구에 비하여 15∼25% 증수되었고, 품질은 처리간에 큰 차 없으나, 개량제 시용으로 대조구에 비하여 3 - 7% 향상되었다. 수익 (10a당 수납대금)은 인산흡수계수에 대한 3 %조절량+퇴비증시구가 가장 높아 대조구보다 34%의 증가를 가져왔다.

• Journal of the Korean Wood Science and Technology
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• 제26권1호
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• pp.76-86
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• 1998

Effects of processing variables and MAPP (maleic anhydride polypropylene) coupling agent on the properties of composite were discussed for turbulent-air-mixed woodfiber-polypropylenefiber composites. In this research, density, composition ratio, and mat moisture content were established as processing variables, and emulsified MAPP prepared by direct pressure method was incorporated as the coupling agent. And the turbulent air mixer, which was improved in function through alteration of our previous fiber mixer, was used to mix wood fibers and polypropylene fibers. At the addition level of 1% MAPP, based on oven-dried wood fiber weight, woodfiber-polypropylenefiber composites generally showed enhanced the physical and mechanical properties. And composites with low to medium densities of 0.6 to 0.8g/$cm^3$ greatly increased in these property values than with high densities of 1.0g/$cm^3$ or more by adding 1 % MAPP. Thus, MAPP addition was thought to be an effective way of enhancing properties for nonwoven web composites. At the mat moisture contents of 5 to 20%, however, the physical and mechanical properties were not enhanced by adding 1% MAPP. In the composites containing 15% polypropylene fibers, the lowest thickness swelling and water absorption values were observed at the 1% MAPP level. The addition of more than 1% MAPP had the adverse effect on the physical and mechanical properties of composites.