• Asian-Australasian Journal of Animal Sciences
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• 제13권4호
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• pp.470-473
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• 2000

The experiment was carried out to evaluate the effect of varying levels of flaked com abomasally infused on energy metabolism and nitrogen metabolism in fattening steers. The starch levels of flaked corn of abomasally infused were 0, 300, 600 and 900 g/d. Four mature fattening steers fitted with permanent abomasum cannulas were allocated to a $4{\times}4$ Latin square design were fed at 1.2 maintenance requirement a basal diet of Chinese wildrye (Aneurolepidium Chinense). Compared with 0 g/d (control group), digestible nitrogen, retention nitrogen (RN, g/d) and the efficiency of digestible nitrogen converted into retention nitrogen (RN/DN, %) of 300, 600 and 900 g/d groups were higher (p<0.05). The post-ruminal starch digestion of flaked corn were 71.36, 80.27 and 64.71 % when the amounts abomasally infused were 300, 600 and 900 g/d, respectively. When the amount of starch abomasally infused was more than 600 g/d, the post-ruminal digestion of starch decreased. 300, 600 and 900 g/d starch infusion groups showed higher metabolizable energy intake (ME) and net energy gains (NEg, MJ/d) than the control group, and the efficiencies of metabolizable energy converted into body weight (Kf, %) of these groups were higher than the control group by 38.31, 73.18 and 67.06% (p<0.05). Kf (Y, %) had a positive curved relation to starch of flaked com abomasally infused (X, g/d), $Y=36.1605X^{0.0760}$ (n=16, r=0.9308).

• 한국임상수의학회지
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• 제11권1호
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• pp.437-470
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• 1994

These studies were performed to establish more effective surgical method for correcting congenital atresia coli in calves. Distal colon fistulation, proximal colon fistulation and colon anastomosis which could be applied for surgical repair of intestinal obstruction were carried out in goatlings experimentally and in calves with atresia coli clinically. After treatment of the animals clinical signs, blood component values, body weight gain, survival and necropsy findings were observed. In goatlings, clinical signs after colon fistulation were diarrhea, anorexia and vitality loss, and those of proximal colon fistulation group were severer than those of distal colon fistulation group. Surviviability after operation was 9~16 weeks in distal colon fistulation group, 2~8 weeks in proximal colon fistulation group, and 2-3 days in colocolic anastomosis group, respectively. There were no alterations in blood component values among experimental groups. Weight gain rates were 54.6% in distal colon fistulation group and 42.9% in proximal colon fistulation group compared with those of control. Necropsy findings observed in experimental groups were distension of intestine and excessive fluid in abomasum and intestine. Two calves with atresia coli died 1 day and 6 days after operation but one with colon fistulation survived more than two months. Preoperative clinical signs in calves with atresia coli were abdominal distension, progressive anorexia, no defecation and postoperative clinical signs wert diarrhea and periodic abdominal distension. After operation there were no alterations in blood component values between the calf with atresia coli and control calf. Weight gain rate of calf with atresia coli was similar to that of control but revealed the tendency to decrease from the 2nd month after operation. Necropsy findings observed in two calves with atresia coli were intestinal distension. intraluminal excessive fluid, blind atretic sac and absence of intrarectal content. It was concluded that proximal or distal colon fistulation could be available for surgical correction of congenital atresla coli and prognosis of distal colon fistulation was better than of proximal colon fistulation, but that extensive colocolic anastomosis could not be compatible with life in ruminants.

• 한국임상수의학회지
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• 제22권4호
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• pp.408-411
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• 2005

Schistosomus reflexus is the most common monstrosity found in cases of bovine dystocia and it occurs mostly in cattle. Caesarian section performed and retracted abnormal foetus from dystocia in Holstein cow. It was the first observed schistosomus reflexus Holstein calf in Korea. The body and chest walls were bent laterally and dorsally leaving the thoracic and abdominal viscera exposed. Clefts of the sternum from part of the defect in which there is, simultaneously, lordosis, dorsal reflection of the ribs and complete eventration of viscera, severe ventral curvature of the spinal column, non-union of the pelvic symphysis and dorsal reflection of the pelvic bones with the occiput of the head lying near the sacrum. The limbs are usually ankylosed. The liver was abnormal in shape and cystic in nature. The lung was smaller and thymus were bigger than those of the normal calf, The ruminant stomach was normal, however, abomasum distended with amnionic fluid. The other organ including heart, spleen and internal organ have normal shape.

• 한국임상수의학회지
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• 제5권1호
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• pp.43-52
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• 1988

These studies were taken experimentally to clarify the clinical and histopathological findings of listeriosis in Korean native goat(KNC). Four KNGs of 4 to 5 months of age were inoculated orally or in-travenously (IV) with Listeria monocytogenes isolated from a field case of KNG. 1. On the clinical findings, depression, anorexia and fever were observed in all inoculated goats, and nasal discharge, keratoconjunctivitis and diarrhea in 3 of 4 goats. Highest rectal temperature after in-oculation was 2.5$^{\circ}C$ higher in IV inoculated goats and 1.9$^{\circ}C$ higher in orally inoculated than normal rectal temperature observed before inoculation. Durations of clinical course after inoculation in IV and orally inoculated goats were 5 days and 8 days, respectively. 2. On the gross lesions, swelling of the lymph nodes, hemorrhage and .inflammation of the small intestine and rigor mortis were observed in 4 of 4 goats, and keratoconjunctivitis, hemorrhage and inflammation of the large intestine, swelling of the spleen, pneumonia and hydropericardium in 3 of 4 goats. Congestion of the visceral organs and ecchymosis of the sin in a fetus were observed. Keratoconjunctivitis, hemorrhage of the abomasum, swelling of the lymph node, hemorrhage and inflammation of the small intestine, swelling of the spleen, necrosis of the liver and pneumonia were observed as severe lesions. These lesions were more severe in IV inoculated goats than those in orally inoculated goats. 3. On histopathological findings, focal necrosis found throughout the livers occurred mainly on peripheral areas of hepatic lobules. These necrotic foci consisted of neutrophils, lymphocytes, macrophages, short rod bacteria and necretic hepatic cells. Suppurative pneumonia of the lung, hyperemia, congestion, epithelial necrosis and sloughing of the small and large intestine, swelling in periventricular regions of the central nervous system, swelling of the kidney, spleen and lymph node were observed as listerial lesions.

• 대한수의학회지
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• 제34권4호
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• pp.695-703
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• 1994

The development of reticulum in fetuses between 60, 90, 120 days of gestation and neonates of Korean native goats was investigated by light, scanning electron microscopy. The results were summarized as follows; 1. In the 60-day-old fetuses, the stomach was developed and differentiated into four compartments of rumen, reticulum, omasum, and abomasum. The reticular epithelial layers were differentiated into two zones; a small dark basal and a large light luminar zones. The wall of reticulum resembled that of the rumen except that the mucosa was in the cranio-dorsal region of the reticulum. 2. In the 90-day-old fetuses, the light luminar zone of the reticulum was about 10-16 times thicker than the dark zone. The outlines of the reticular ribs were visible. 3. In the 120-day-old fetuses, the wall of the reticlum had also increased in thickness. The reticular mucosa exhibited an irregular luminar surface and the invaginations had differentiated into large regularly arranged ones separated by 3-5 and small irregularly arranged ones. 4. In the neonate, the luminar surface of the reticular mucosa demonstrated clear furrows, at which the superficial cells of the light zone had undergone degenerative changes. 5. Scanning electron microscopic studies; In the 60-day-old fetuse, numerous microvilli were observed on the superficial epithelial layer of shape or dome like at 120 days. In the neonate, the reticular papillae liked the little finger.

• 대한수의학회지
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• 제34권4호
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• pp.687-694
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• 1994

The development of rumen in fetuses between 60, 90, 120 days of gestation and neonates of Korean native goats was investigated by light, scanning electron microscopy. The results were summarized as follows; 1. In the 60-day-old fetuses, the stomach was developed and differentiated into four compartments of rumen, reticulum, omasum, and abomasum. The ruminal epithelial layers were differentiated into two zones; a small dark basal and a large light luminar zones. 2. In the 90-day-old fetuses, the light luminar zone of the rumen was 6-12 times thicker than the dark zone. 3. In the 120-day-old fetuses, the wall of the rumen had increased in thickness, and its the mucosa was still smooth. Several undulations of various depths involving the basal zone, basement membrane and lamina propria were observed at irregular within the light zone. 4. In the neonate, the wall of the rumen had increased in thickness and the luminar surface had become wavy due to the appearance of several shallow furrows in the luminar surface of the epithelium especially between the tips of the relatively more developed undulations. 5. Scanning electron microscopic studies: In the 90-day-old fetuses, numerous microvilli and microridges were observed on the superficial epithelial layer of the rumen. The ruminal papillae were already formed at 120 days of gestation. In the 120-day-old fetuses, the ruminal papillae formed short dome-shape. In the neonate, the ruminal papillae formed pebble-shape or short finger like.

• Asian-Australasian Journal of Animal Sciences
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• 제29권9호
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• pp.1363-1370
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• 2016

Rennet, a complex of enzymes found in the stomachs of ruminants, is an important component for cheese production. In our study, we described that yak chymosin gene recombinant Pichia pastoris strain could serve as a novel source for rennet production. Yaks total RNA was extracted from the abomasum of an unweaned yak. The yak preprochymosin, prochymosin, and chymosin genes from total RNA were isolated using gene specific primers based on cattle chymosin gene sequence respectively and analyzed their expression pattern byreal time-polymerase chain reaction. The result showed that the chymosin gene expression level of the sucking yaks was 11.45 times higher than one of adult yaks and yak chymosin belongs to Bovidae family in phylogenetic analysis. To express each, the preprochymosin, prochymosin, and chymosin genes were ligated into the expression vector $pPICZ{\alpha}A$, respectively, and were expressed in Pichia pastoris X33. The results showed that all the recombinant clones of P. pastoris containing the preprochymosin, prochymosin or chymosin genes could produce the active form of recombinant chymosin into the culture supernatant. Heterologous expressed prochymosin (14.55 Soxhlet unit/mL) had the highest enzyme activity of the three expressed chymosin enzymes. Therefore, we suggest that the yak chymosin gene recombinant Pichia pastoris strain could provide an alternative source of rennet production.

• 한국임상수의학회지
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• 제1권1호
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• pp.11-23
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• 1984

Epizootiolosical factors of 166 cows with abomasal displacement were studied and in 21 out of 166 cows, the functions of liver and kidney were also tested. 1. Incidence of abomasal displacement was perdominant in small sized dairy herd less than 15 cows, using area of paddock below 165㎡. 2. Out of 166 cows with abomasal displacement, 116 cows (70%) were found left sided displacement and 50 cows (30%) were right. 3. Generally, one to two weeks were taken before treatment of veterinarian following onset of sign of the diseases. Therefore many cases were found to be severe in their illness. 4, Incidence rate of abomasal displacement was predominant in summer season (June, July and Au-gust). However, occurence of the disease was continuous throughout the year. About 80 percent of abomasal displacement was distributed from first to third purturition. 5. High milk production and feeding with high concentrates and low roughage showed a tendendy to occurs the disease. 6. Approximately 75% of abomasal displacement was distributed within 1 month pre and post partum. 7. Cows with abomasal displacement consumed little concetrates and 75% of cows with abomasal displacement passed abnormal fecal material. 8. Sixty six out of 166 cows with abomsal displacement were coincident with diseases such as retained placenta, metritis, traumatic reticulo-peritonitis and mastitis. 9. In many cass of abomasal displacement, abomasum was extended with gas. 10. Activities of AST ana LDH showed the trends to reduce after surgical intervention comparing with pre-surgery. Bilirubin concentration markedly decreased after surgical treatment comparing with pre-surgery. 11. The concentration of BUN and creatinine moderatly decreased after surgery compared with pre-surgery.

• 한국동물위생학회지
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• 제30권2호
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• pp.219-231
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• 2007

The effects of intraduodenal administration of Clostridium perfringens type D whole culture in goats were evaluated to develop a reliable experimental model of enterotoxemia in this species and the eventual evaluation of treatment with different drug preparations was also carried out. A total of 28 conventionally reared healthy unvaccinated black bangle goat kids of 6-12 months of age were dosed intraduodenally with whole cultures of C peliringens type D. Four kids were used as controls and received sterile, nontoxic culture medium intraduodenally. All animals received starch solution into the abomasum. The clinical signs developed within 12 hours of post inoculation that were similar to those observed in naturally occurring cases. Among the clinical signs, diarrhea was most common (96.43%) followed by dyspnea (53.57%) and central nervous system (CNS) signs (25.0%). The most striking postmortem findings consisted of necrotizing pseudomembranous colitis (100.0%), lung edema (69.23%) and fluid filled intestines (61.53%). The protocol thus provided a reasonable model of naturally occurring enterotoxemia in goats, producing a range of clinical signs and postmortem changes similar to those observed in the natural disease. Beside this, treatment trial with different drug preparations showed penicillin combined with antitoxin was most effective (100.0%), followed by combination of oxytetracyclin with antitoxin, and combined preparation of antitoxin and sulfur drugs both showed 75% recovery rate. On the other hand, treatment with antitoxin, penicillin and oxytetracycline singly could protect goat enterotoxaemia only 25.0%, 50.0% and 50.0%, respectively. Thus in the present study, it eas observed that antisera in combination of antibiotics gave better recovery rate than the antitoxin or antibiotics alone.

• 대한수의학회지
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• 제35권1호
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• pp.1-9
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• 1995

The development of omasum in fetuses between 60, 90, 120 days of gestation and neonates of Korean native goats was investigated by fight; scanning electron microscopy. The results were summarized as follows : 1. In the 60-day-old fetuses, the stomach was developed and differentiated into four compartments of rumen, reticulum, omasum, and abomasum. The mucosa of the omasum was thrown into folds which appeared to be three or four different orders of large, medium, small, and smallest laminae. 2. In the 90-day-old fetuses, the omasal laminae had increased greatly in length and cores of omasal laminae were invaginated laterally. 3. In the 120-day-old fetuses, the wall of the omasal laminae had increased in length and the lamina epithelialisis had slightly increased in thickness. The lateral invaginations at the dark zone had increased in length and depth. The tunica muscularis increased only slightly in thickness particularly within its inner circular layer. 4. In the neonate, the muscularis and the omasal laminae had increased in thickness. The lateral invaginations of the dark zone were observed in the first, second and third order of omasal laminae. 5. Scanning electron microscopic studies : In the 90-day-old fetuses, numerous microvilli and microridges were observed on the luminar surface of the omasal mucosa. The omasal papillae were already formed at 120 days of gestation. In the I20-day-old fetuses omasal papillae liked nipple shape. In the neonate, the omasal papillae liked the thumb.