• 한국자원식물학회지
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• 제23권6호
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• pp.535-540
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• 2010

Phenylalanine ammonia-lyase (PAL), a key enzyme of the phenylpropanoid biosynthesis pathway, is activated by a number of developmental and environmental cues. The coding region of the NtPAL4 gene was 2,154 bp in length, and its deduced protein was composed of 717 amino acids. Sequence analysis of NtPAL4 cDNA from tobacco (Nicotiana tabacum L.) revealed high structural similarity to PAL genes of other plant species. The NtPAL4 gene exists as a single copy in the tobacco plant, and its transcripts were strongly expressed in flowers and leaves. NtPAL4 expression was significantly induced in response to NaCl, mannitol, and cold treatments, but it was not induced by abscisic acid (ABA). NtPAL4 expression decreased gradually after treatment with ABA and $H_2O_2$; however, NtPAL4 transcripts accumulated after treatment with methyl viologen (MV). Our results suggest that the NtPAL4 gene may function in response to abiotic stresses.

• BMB Reports
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• 제41권4호
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• pp.338-343
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• 2008

Dehydrins (DHNs) compose a family of intrinsically unstructured proteins that have high water solubility and accumulate during late seed development at low temperature or in water-deficit conditions. They are believed to play a protective role in freezing and drought-tolerance in plants. A full-length cDNA encoding DHN (designated as ClDhn) was isolated from an oriental medicinal plant Codonopsis lanceolata, which has been used widely in Asia for its anticancer and anti-inflammatory properties. The full-length cDNA of ClDhn was 813 bp and contained a 477 bp open reading frame (ORF) encoding a polypeptide of 159 amino acids. Deduced ClDhn protein had high similarities with other plant DHNs. RT-PCR analysis showed that different abiotic stresses such as salt, wounding, chilling and light, triggered a significant induction of ClDhn at different time points within 4-48 hrs post-treatment. This study revealed that ClDhn assisted C. lanceolata in becoming resistant to dehydration.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.165-165
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• 2017

Abiotic stress is one of the major serious limiting factors in rice (Oryza sativa) and caused rice production losses. It is important to precisely screen valuable genetic resources for improving stress tolerance and understanding tolerance mechanism to abiotic stresses. Because there are differences of experiment designs for screening of tolerant plant in several studies related to abiotic stress, this study has performed to provide the rapid and efficiency screening method for selection of tolerance rice to drought and salinity stresses. Two week-old rice seedlings that reached about three leaf stage were treated with drought and salinity stresses and examined tolerant levels with tolerant and susceptible control varieties, and transgenic plants. To determine the optimum concentration for the selection of drought and salinity condition, tolerant, susceptible and wild-type plants were grown under three soil moisture contents (5, 10 and 20% water contents) and three NaCl concentrations (100, 200 and 250 mM) for 10 days at seedling stage. 200 mM NaCl concentration and 5% moisture content soil were determined as the optimum conditions, respectively. The described methodologies in this study are simple and efficiency and might help the selection of drought and salinity tolerance plants at the 3,4-leaf-seedling stage.

• The Plant Pathology Journal
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• 제28권1호
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• pp.101-106
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• 2012

Trifloxystrobin is a strobilurin fungicide, which possesses broad spectrum control against fungal plant diseases. We demonstrated that pre-treating red pepper plants with trifloxystrobin resulted in increased plant growth and leaf chlorophyll content compared with those in control plants. Relative water content of the leaves and the survival rate of intact plants indicated that plants acquired systemic tolerance to drought stress following trifloxystrobin pre-treatment. The recovery rate by rehydration in the drought treated plant was better in those pre-treated with trifloxystrobin than that in water treated plants. Induced drought tolerance activity by trifloxystrobin was sustained for 25 days after initial application. The trifloxystrobin treated red pepper plants also had induced systemic tolerance to other abiotic stresses, such as frost, cold, and high temperature stresses. These findings suggest that applying the chemical fungicide trifloxystrobin induced systemic tolerance to certain abiotic stresses in red pepper plants.

• Journal of Plant Biotechnology
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• 제40권1호
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• pp.49-54
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• 2013

C3HC4-type RING zinc finger proteins essential in the regulation of plant processes, including responses to abiotic stresses. We previously isolated and examined the C3HC4-type RING zinc finger protein (BrRZFP1) from Brassica rapa under abiotic stresses. To elucidate the role of the BrRZFP1 transcription factor in gene regulation, we transformed tobacco plants with the BrRZFP1 gene. Plants were regenerated from 82 independently transformed callus lines of tobacco and analysed for transgene expression. Transgene integration and expression was confirmed by Southern and RT-PCR analyses, respectively. T2 plants displayed more tolerance to the bacterial pathogens Pectobacterium carotovorum and Ralstonia solanacearum, and the tolerance levels were correlated with BrRZFP1 expression levels. These results suggest that the transcription factor BrRZFP1 is an important determinant of stress response in plants and its overexpression in plants could increase biotic stress resistance.

• Journal of Microbiology and Biotechnology
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• 제28권7호
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• pp.1217-1224
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• 2018

Seaweeds produce antioxidants to counteract environmental stresses, and these antioxidant genes are regarded as important defense strategies for marine algae. In this study, the expression of Pyropia yezoensis (Bangiales, Rhodophyta) ascorbate peroxidase (PyAPX) and manganese-superoxide dismutase (PyMnSOD) was examined by qRT-PCR in P. yezoensis blades under abiotic stress conditions. Furthermore, the functional relevance of these genes was explored by overexpressing them in Chlamydomonas. A comparison of the different expression levels of PyAPX and PyMnSOD after exposure to each stress revealed that both genes were induced by high salt and UVB exposure, being increased approximately 3-fold after 12 h. The expression of the PyAPX and PyMnSOD genes also increased following exposure to $H_2O_2$. When these two genes were overexpressed in Chlamydomonas, the cells had a higher growth rate than control cells under conditions of hydrogen peroxide-induced oxidative stress, increased salinity, and UV exposure. These data suggest that Chlamydomonas is a suitable model for studying the function of stress genes, and that PyAPX and PyMnSOD genes are involved in the adaptation and defense against stresses that alter metabolism.

• Journal of Plant Biotechnology
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• 제38권2호
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• pp.151-161
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• 2011

Plant proteomics is the large-scale studies of proteins, particularly on their structures and functions, governed by the physiological metabolism of plant cells. With the development of techniques and strategies in proteomics, proteomics approach is moving forward in systems biology handling sophisticated components of major signaling and biochemical pathways in plants responding to their environment. In Korea, pioneers in plant proteomics are trying to catch up with global trends in plant proteomics; these researchers are not only improving existing techniques in protein extraction but also developing new techniques in proteomics context. In the hot field of abiotic and biotic stress proteomics, 29 and 9 out of 74 papers have been published during the review period from 2005 to 2010, respectively. This present review article provides an overview on the output of Korean plant proteomers while paying special attention to both abiotic and biotic stress proteomics.

• Journal of Applied Biological Chemistry
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• 제55권2호
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• pp.71-77
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• 2012

Many of biochemical or physiological processes can be regulated by non-coding RNAs as well as coding RNAs in plants, animals and microbes. Recently, many small RNAs including microRNAs (miRNAs) and endogenous small interference RNAs (siRNAs) and long non-coding RNAs have been discovered from ubiquitous organisms including plants. Biotic and abiotic stresses are main causal agents of crop losses all over the world. Much efforts have been performed for understanding the complex mechanism of stress responses. Up to date, many of these researches have been related with the identification and investigation of stress-related proteins, showing limitation to resolve the complex mechanism. Recently, non-coding RNAs as well as coding genes have been gradually interested because of its potential roles in plant stress responses as well as other biophysical aspects. In this review, various potential roles of non-coding RNAs, especially miRNAs and siRNAs, are reviewed in relation with plant biotic and abiotic stresses.

• 한국초지조사료학회지
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• 제43권1호
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• pp.42-49
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• 2023

The plant-specific NAC transcription factors control various biological processes, including plant development and stress responses. We have isolated an ANAC032 gene, one of the NAC transcription factor family, which was highly activated by multi-abiotic stresses, including high salt and drought in Arabidopsis. Here, we generated transgenic plants constitutively expressing ANAC032 and its knockout to identify the functional roles of ANAC032 in Arabidopsis under abiotic stress responses. The ANAC032-overexpressing plants showed enhanced tolerance to salinity and drought stresses. The anac032 knockout mutants were observed no significant changes under the high salt and drought conditions. We also monitored the expression of high salt and drought stress-responsive genes in the ANAC032 transgenic plants and anac032 mutant. The ANAC032 overexpression upregulated the expression of stress-responsive genes, RD29A and ERD10, under the stresses. Thus, our data identify that transcription factor ANAC032 plays as an enhancer for salinity and drought tolerance through the upregulation of stress-responsive genes and provides useful genetic traits for generating multi-abiotic stress-tolerant forage crops.

• 생명과학회지
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• 제30권6호
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• pp.570-579
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• 2020

Pre-mRNA의 스플라이싱은 진핵생물 유전자의 적절한 발현에 매우 중요한 역할을 한다. 선택적 스플라이싱은 스플라이싱 위치가 서로 다르게 인식될 때 발생하며 동일한 pre-mRNA로부터 둘 이상의 전사체와 단백질을 생성할 수 있다. 스플라이싱 위치의 결정은 스플라이소솜과 SR 단백질, hnRNP, CBP 등의 스플라이싱 인자에 의해 조절된다. 고온, 저온, 고염, 건조, 저산소 등 다양한 환경 스트레스 조건에서 식물의 많은 스트레스 반응 유전자에 대해 선택적 스플라이싱이 일어나는 것이 알려져 있으며, 이러한 선택적 스플라이싱은 식물이 환경 변화에 적응하기 위한 중요한 기작 중 하나로 여겨진다. 저온, 고온, 고염, 건조 스트레스 조건에서는 스플라이싱 인자의 발현이 변하거나 또는 정상 조건에서와는 다른 스플라이싱 활성을 가짐으로써 선택적 스플라이싱이 일어난다. 환경 스트레스 반응 유전자의 스플라이싱 이소형은 각각 환경 스트레스에 대해 서로 다른 반응을 보이는데 생성되는 조직이 서로 다르기도 하고, 일부 이소형은 넌센스-매개 분해에 의해 분해되기도 한다. 스플라이싱 이소형의 단백질은 환경 스트레스 조건에서 정상 조건과 비교하여 세포 내 위치가 다르기도 하고, 전사인자 또는 효소로서 다른 활성을 가지기도 한다. 이러한 다양한 연구에도 불구하고 식물의 환경 스트레스 반응에서 선택적 스플라이싱에 대한 연구는 일부 스트레스와 유전자에 국한 되어 있고, 아직 분자 기전이 제대로 밝혀지지 않은 부분이 많아 앞으로 더 많은 연구가 필요하다.