• The Plant Pathology Journal
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• v.28 no.3
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• pp.259-269
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• 2012

Protein phosphatase 2A (PP2A), a family of serine/threonine protein phosphatases, plays an important role in balancing the phosphorylation status of cellular proteins for regulating diverse biological functions in eukaryotic organisms. Despite intensive studies in mammals, limited information on its role is available in filamentous fungi. Here, we investigated the functional roles of genes for a putative B' delta regulatory subunit (FgPP2AR) and a catalytic subunit (FgPP2AC) of PP2A in a filamentous ascomycete, Fusarium graminearum. Molecular characterization of an insertional mutant of this plant pathogenic fungus allowed us to identify the roles of FgPP2AR. Targeted gene replacement and complementation analyses demonstrated that the deletion of FgPP2AR, which was constitutively expressed in all growth stages, caused drastic changes in hyphal growth, conidia morphology/germination, gene expression for mycotoxin production, sexual development and pathogenicity. In particular, overproduction of aberrant cylindrical-shaped conidia is suggestive of arthroconidial induction in the ${\Delta}FgPP2AR$ strain, which has never been described in F. graminearum. In contrast, the ${\Delta}FgPP2AC$ strain was not significantly different from its wild-type progenitor in conidiation, trichothecene gene expression, and pathogenicity; however, it showed reduced hyphal growth and no perithecial formation. The double-deletion ${\Delta}FgPP2AR;{\Delta}FgPP2AC$ strain had more severe defects than single-deletion strains in all examined phenotypes. Taken together, our results indicate that both the putative regulatory and catalytic subunits of PP2A are involved in various cellular processes for fungal development in F. graminearum.

• Korean Journal of Veterinary Research
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• v.41 no.2
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• pp.167-172
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• 2001

The present study was conducted to examine morphological and biological characteristics of 57 strains of Microsporum canis isolated from 110 dogs with dermatophytosis in Taegu, 1999. Isolated strains of M canis were classified into 4 types(A: white-yellow type, B: fluffy type, C: flat type, D: radial groove type) according to their pigmentation and morphology of colonies with typical conidia and hyphae. Some strains of A and B type happened variation by subculture for 6~8 months. The variation strains were classified into 3 types(E, F, G), E type had aberrant macroconidia with typical macroconidia, F type had aberrant hyphae(like dumb-bell) and macroconidia, and G type had no conidia and racket hyphae. All strains showed positive urease test, rice medium growth test and hair perforation test.