• Journal of fish pathology
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• v.37 no.1
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• pp.139-145
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• 2024

In this study, nylon mash and silicone tube mainly used as shrimp farm equipment were contaminated with Enterocytozoon hepatopenaei (EHP) which is the cause of Hepatopancreatic microsporidiosis (HPM), and were treated with calcium hypochlorite or UV-C disinfection methods for EHP eradication. As a result, similar with the control group (not disinfected), EHP was detected on the nested PCR until the 10 days in the UV-C single treated group. On the other hand, EHP was not detected from 7 days in calcium hypochlorite single treated group (total concentration 200 ppm as available chlorine), and combination of calcium hypochlorite and UV-C treated group revealed no detection of EHP from 3 days. It is appropriate that treated with UV-C and calcium hypochlorite for 3 days or single treated with calcium hypochlorite for 7 days to eradicate EHP on contaminated instrument used in shrimp farms. In contrast, disinfection effect of only using UV-C is very low.

• Proceedings of the Korean Institute of Interior Design Conference
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• 2007.11a
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• pp.59-62
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• 2007

A tendency of active exchanges of newly-generated culture and mind following with the world globalization has been leading totally new concept of social level, its life style and spending habit. According to these social movements, people's way of thinking has been shifted from we-centered to I-centered, which generate (cause) individualism. The tendency of putting much weight on individuality prevailed in the society grows the social awareness on individual's needs and tastes and ultimately lead overall movement of longing for single life style. The rate for singles also has been dramatically increased these days, and expects reach 30 percents of overall popularity in 2010. Therefore, various new concepts of residences for singles have been introduced recently, such as an office apartment, condominium and serviced residents, which combines concepts of both a hotel and an apartment. In this thesis, six examples of newly-styled residence for singles would be researched in every aspect, such as its service, facilities and so on. Although the fact of that overall service quality of new type residence now is well constructed and performed is derived from this research, I expect to contribute more various and advanced service quality into new social movement and new type of residences for single life, and finally provide satisfactory life style for singles.

• Journal of Families and Better Life
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• v.20 no.6
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• pp.219-227
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• 2002

This study was conducted to examine housing preferences among single-person households and to propose guidelines for housing design choices for single people. One hundred and sixty responses to the questionnaire developed by this researcher were analyzed utilizing frequencies, percentages, and t-tests. There were significant differences between single households and non-single households in various aspects of housing preferences. Differences were found in the desired type and size of housing, needed amount of space, and the importance of space itself. Single persons wanted to live in a small apartment, about 83.45$m^2$ (25.29 pyung) size. They consider the bathroom and the master bedroom more important than the living room and kitchen. They prefer to have specifically function-oriented rooms such as shower, home office, computer room, exercise room, or leisure space. Single persons consider information-technological facilities within an apartment very important. They would like to adopt automatic and electronic technology in the house, such as optical communication network, automatic air-cleaning system, and internet TV. Housing preferences differ significantly according to socio-demographic variables such as gender and age. I propose in this study a small-sized, information-, high-tech- and leisure-oriented design that corresponds with the actual housing preferences of the single-person households.

• Journal of Families and Better Life
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• v.14 no.4
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• pp.263-276
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• 1996

The purpose of this study is to explore various environmental factors that explain parenting practices. Those factors considered are stress and characteristics of single-mother/child together with psychological distress experienced by the single mother. A total of 285 poor single mothers with a child attending elementary school or junior high school completed a structured questionaire. The relationships among the variables were analyzed by the path analysis. The results showed that poor single mothers rely on affectiv $e^portive involvement more frequently then punitive/inconsistent discipline. It is also confirmed that the enviromental factors such as financial stress living environmental stress and job status play important roles in determining the quality of parenting practices with the mediating role of psychological distress.ss.

• Journal of Microbiology and Biotechnology
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• v.25 no.8
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• pp.1371-1379
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• 2015

The Ca_v1.2 Ca²⁺ channel is essential for cardiac and smooth muscle contractility and many physiological functions. We mutated single, double, and quadruple sites of the four potential Asn (N)-glycosylation sites in the rabbit Ca_v1.2 into Gln (Q) to explore the effects of Nglycosylation. When a single mutant (N124Q, N299Q, N1359Q, or N1410Q) or Ca_v1.2/WT was expressed in Xenopus oocytes, the biophysical properties of single mutants were not significantly different from Ca_v1.2/WT. In comparison, the double mutant N124,299Q showed a positive shift in voltage-dependent gating. Furthermore, the quadruple mutant (QM; N124,299,1359,1410Q) showed a positive shift in voltage-dependent gating as well as a reduction of current. We tagged EGFP to the QM, double mutants, and Ca_v1.2/WT to chase the mechanisms underlying the reduced currents of QM. The surface fluorescence intensity of QM was weaker than that of Ca_v1.2/WT, suggesting that the reduced current of QM arises from its lower surface expression than Ca_v1.2/WT. Tunicamycin treatment of oocytes expressing Ca_v1.2/WT mimicked the effects of the quadruple mutations. These findings suggest that Nglycosylation contributes to the surface expression and voltage-dependent gating of Ca_v1.2.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.13
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• pp.5287-5291
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• 2014

Background: The cancer survival rate in Korea has substantially increased, necessitating the management of not only patients with cancer but also longer term survivors. Although the divorce rate has drastically increased in Korea, there is not sufficient research regarding the relationship between changes in marital status and quality of life (QOL) in cancer patients and survivors. Thus, we aimed to examine the relationship between marital status and QOL in such cases. Materials and Methods: This study was performed using the Community Health Survey of 2008 administered by the Korea Centers for Disease Control and Prevention (N=169,328). We used t-tests and Chi-square tests to compare demographic variables between men and women, and analysis of variance (ANOVA) to compare QOL scores among comparison groups. We also performed a multilevel analysis on the relationship between QOL and marital status while accounting for provincial differences. Results: Decline of EuroQOL five dimensions (EQ-5D) in single patients with cancer was greater than in any other marital status group, but there was no statistically significant decline in survivors of cancer with regard to marital status. In the general population, the decline of EQ-5D was higher among single people than married people. Using the EuroQOL visual analog scale (EQ-VAS), single people had higher values than those of other marital status among both patients with cancer and survivors of cancer. In the general population, EQ-VAS values were higher for single people compared to married people. Conclusions: There may be a significant relationship between marital status and QOL in cancer patients and survivors. Policy interventions to manage patients with cancer who experience a decline in QOL as well as marital problems should be conducted.

• 한국생물공학회:학술대회논문집
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• 2005.10a
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• pp.622-622
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• 2005

• Journal of Families and Better Life
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• v.35 no.2
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• pp.93-112
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• 2017

The adolescent birth rate has doubled in the recent decade. As a result, the Korean government has implemented support for adolescent single-parent families by expanding the scope of coverage of the Single-parent Family Support Act in 2010. In order to understand whether experiences of facility entry programs and the Single-parent Family Support Act for adolescent single-parents were helpful or not, this study verified the relationship to mental health. This study utilized a part of the National Youth Policy Institute's 'Research on the Actual Condition of the Adolescent Pregnancy, Birth and Rearing Children.' Participants of the study were 218 adolescent single-parents at the age of 24 and younger. This study used the research methods of descriptive statistical analysis, ${\chi}^2$test, t-test, and one-way analysis of variance. The findings and discussion of this study are as below: Firstly, the depression level of adolescent single-parents who had been raised by grandparents was higher than other family types to a meaningful standard and adolescent single-parent's cognition of the original family's economic level was related to self-esteem and parenting efficacy. Secondly, the practical single-parent family support policy for adolescent single-parent's pregnancy, birth and rearing children has shown a higher reception rate, and medical service of facility entry programs has a higher satisfaction level. However, some of the facility entry programs and the Single-parent Family Support Act have a low reception rate and it should be considered whether to maintain them or not.Lastly, parts of facility entry programs and the Single-parent Family Support Act for adolescent single-parents have increased self-esteem and parenting efficacy and reduced parenting stress and depression's levels.

• Korean Journal of Breeding Science
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• v.43 no.2
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• pp.115-119
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• 2011

Soybean proteins are widely used for human and animal feeds worldwide. The use of soybean protein has been expanded in the food industry due to their excellent nutritional benefits. But, antinutritional and allergenic factors are present in the raw mature soybean. P34 protein, referred as Gly m Bd 30K, has been identified as a predominant immunodominant allergen. The objective of this research is to identify the genetic mode of P34 protein for the improvement of soybean cultivar with a very low level of P34 protein. Two $F_2$ populations were developed from the cross of "Pungsannamulkong" ${\times}$ PI567476 and "Gaechuck2ho" ${\times}$ PI567476 (very low level of P34 protein). Relative amount of P34 protein was observed by Western blot analysis. The observed data for the progeny of "Pungsannamulkong" and PI567476 were 133 seeds with normal content of P34 protein and 35 seeds with very low level of P34 protein (${\chi}^2=1.157$, P=0.20-0.30). For the progeny of "Gaechuck#1" and PI567476, the observed data were 177 seeds with normal content of P34 protein and 73 seeds with very low level of P34 protein (${\chi}^2=2.353$, P=0.10-0.20). From pooled data, observed data were 310 seeds with normal content of P34 protein and 108 seeds with very low level of P34 protein (${\chi}^2=0.156$, P=0.50-0.70). The segregation ratio (3:1) and the Chi-square value obtained from the two populations suggested that P34 protein in mature soybean seed is controlled by a single major gene. Single gene inheritance of P34 protein was confirmed in 32 $F_2$ derived lines in $F_3$ seeds, which were germinated from the low level of P34 protein obtained from the cross of "Pungsannamulkong" and PI567476. These results may provide valuable information to breed for new soybean line with low level of P34 protein and identification of molecular markers linked to P34 locus.

• Horticulture, Environment, and Biotechnology : HEB
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• v.59 no.6
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• pp.865-873
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• 2018

Allium ochotense and Allium microdictyon are commonly known as 'Mountain garlic' and are popular, economically important species in many countries such as Korea, China, and Mongolia. Their leaves are used as culinary side dishes and in traditional medicines. In Korea, these two species are at risk of extinction due to damage to their natural habitat and thus, conservation and breeding programs are needed. However, their identification relies mostly on morphological data, which is limited and until recently, led to classifying these two species under A. victorialis. In the present study, a simple and reliable method of molecular identification was developed to distinguish A. ochotense from A. microdictyon that targets four barcoding regions: the internal transcribed spacer (ITS), the maturase K gene (matK), the chloroplast psbA-trnH intergenic region, and the ribulose-bisphosphate carboxylase large subunit gene (rbcL). Single nucleotide polymorphisms (SNPs) were found in ITS and matK regions, and species-specific primers were designed based solely on the SNP at position 680 of the ITS region that could differentiate A. ochotense from A. microdictyon. Using these primers in amplification refractory mutation system (ARMS)-PCR, A. ochotense, and A. microdictyon could be simultaneously and efficiently distinguished. This study is the first to report a simple, rapid, and efficient method for discriminating A. ochotense and A. microdictyon, indicating the utility of species-specific markers in the development of conservation and breeding programs.