• Korean Journal of Microbiology
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• v.53 no.2
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• pp.71-78
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• 2017

Raman microspectroscopy is a promising tool for microbial analysis at single cell level since it can rapidly measure the cell materials including lipids, nucleic acids, and proteins by measuring the inelastic scattering of a molecule irradiated by monochromatic lights. Using Raman spectra provides high specificity and sensitivity in classification of bacteria at the strain level. In addition, a Raman approach coupled with stabled isotope such as $^{13}C$ and $^2H$ is able to detect and quantify general metabolic activity at single cell level. After bacterial detection process by Raman microspectroscopy, interested unculturable cell sorting and single cell genomics can be accomplished by combination with optical tweezer and microfluidic devices. In this review, the characteristics and applications of Raman microspectroscopy were reviewed and summarized in order to provide a better understanding of microbial analysis using Raman spectroscopy.

• Journal of the Optical Society of Korea
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• v.14 no.4
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• pp.431-437
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• 2010

A stack of gradient-index (GRIN) rod lenses cannot be used for coherent anti-Stokes Raman scattering (CARS) microendoscopy for insertion to internal organs through a surgical keyhole with minimal invasiveness. That's because GRIN lens has large amount of inherent chromatic aberrations in spite of absolutely requiring a common focus for pump and Stokes beam with each frequency of ${\omega}_p$ and ${\omega}_S$. For this endoscopic purpose, we need to develop a long slender probe-type objective, namely probe-type microscope objective (PMO). In this paper, we introduce the structure, the working principle, and the design techniques of PMO which is composed of a probe-type lens module (PLM) and an adaptor lens module (ALM). PLM is first designed for a long slender type and ALM is successively designed by using several design parameters from PLM for eliminating optical discords between scanning unit and PLM. A combined module is optimized again to eliminate some coupling disparities between PLM and ALM for the best PMO. As a result, we can obtain a long slender PMO with perfectly diffraction-limited performance for pump beam of 817 nm and Stokes beam of 1064 nm.

• The Bulletin of The Korean Astronomical Society
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• v.43 no.2
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• pp.39.2-39.2
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• 2018

RR Tel is an interacting binary system in which a hot white dwarf (WD) accretes matter from a Mira variable via gravitational capture of the stellar wind. We present a high-resolution optical spectrum of RR Tel obtained with MIKE at Magellan-Clay telescope, Chile. We find broad emission features at 6825, 7082, 7023, and $7053{\AA}$, which are formed through Raman scattering of far-UV O VI ${\lambda}{\lambda}$ 1032 and $1038{\AA}$, C II ${\lambda}{\lambda}$ 1036 and $1037{\AA}$ with atomic hydrogen. Raman O VI 6825 and 7082 features are characterized by double-peaked profiles indicative of an accretion flow with a characteristic speed ~ 30km/s, whereas the Raman C II features exhibit a single Gaussian profile with FWHM ${\sim}10{\AA}$. Monte Carlo simulations for Raman O VI and C II are performed by assuming that the emission nebula around the WD consists of the inner O VI disk with a representative scale of 1 AU and the outer part with C II sphere. The best fit for Raman profiles is obtained with an asymmetric matter distribution of the O VI disk, the mass loss rate of the cool companion ${\dot{M}}{\sim}2{\times}10^{-6}M_{{\odot}/yr}$ and the wind terminal velocity v~10 km/s. We also find O VI doublet at 3811 and $3834{\AA}$, which are blended with other emission lines. Our profile decomposition shows that the O VI ${\lambda}{\lambda}$ 3811, 3834 doublet have a single Gaussian profile with a width ~ 25 km/s. A comparison of the restored fluxes of C II ${\lambda}{\lambda}$ 1036 and 1037 from Raman C II features with the observed C II ${\lambda}1335$ leads to an estimate of a lower bound of N(CII) > $9.87{\times}10^{13}cm^{-2}$ toward RR Tel, which appears consistent with the presumed distance D ~ 2.6 kpc.

• Publications of The Korean Astronomical Society
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• v.15 no.spc1
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• pp.147-151
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• 2000

Symbiotic stars, believed to be binary systems of a mass-losing giant and a white dwarf with an emission nebula, are known to exhibit very broad wings around Hex that extend to $\~10^3km\;s^{-1}$. The wing formation mechanism is not a settled matter and recently Lee (2000) proposed that Raman scattering of Ly$\beta$ by neutral hydrogen is responsible for the broad H$\alpha$ wings. In this model, it is predicted that. the Hex wings will be polarized depending on the geometric and kinematic distribution of the scatterers relative to the UV emission region. In this paper, we investigate the polarization of Hex wings in symbiotic stars. Noting that many symbiotic stars possess bipolar nebular morphology, we assume that the distribution of neutral scatterers follows the similar pattern with a receding velocity of several tens of km $s^{-1}$ that mimics the expansion of the neutral envelope of the nebula. It is found that the red wing is more strongly polarized than the blue and main part and that the polarization direction is along the equatorial plane. We obtain a typical degree of polarization $\~10$ percent, however, it varies depending on the detailed distribution of H I scatterers We conclude that spectropolarimetry will provide very important information on the origin of the Hex wings.

• Korean Journal of Optics and Photonics
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• v.18 no.4
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• pp.286-291
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• 2007

We theoretically investigated the far-field radiation pattern of epi-signal from a polystyrene sphere in coherent anti-Stokes Raman scattering (CARS) microscopy with an objective lens of high numerical aperture. We calculated the field distribution of the incident laser beams under the tight-focusing condition and the far-field radiation pattern through coherent addition of radiation from the nonlinear polarizations (Hertzian dipoles) as the origin of CARS signal generation. The epi-radiation patterns for polystyrene spheres of different diameters are calculated, and the pattern of a sphere is also compared with that of a shell fer a diameter of 1100 nm. We finally show how the radiation pattern of the polystyrene sphere changes as the center of the sphere shifts from the focus of the beam.

• Korean Journal of Optics and Photonics
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• v.10 no.2
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• pp.95-101
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• 1999

We have studied the 1.54$\mu\textrm{m}$ forward and backward stimulated Raman scattering (SRS) and stimulated Brillouin scattering (SRS) for various $CH_4$pressures by 1.06$\mu\textrm{m}$ Q-switched Nd:YAG laser pumping under a repetition rate of Hz in single pass. We obtained that the output of backward SRS was more efficient than that of the forward SRS. The output energy and conversion efficiency of forward and backward SRS were higher than those of SBS since SRS is a steady state, but SBS is a transient state. In a $CH_4$gas uncirculating system, the output energy of the backward SRS and SBS were reduced the about 47% due to a thermal heating of $CH_4$medium in a focusing region for a repetition rate of 5 Hz. But, the output energy of forward SRS was slightly enhanced by about 8.5% due to the increase of the undepleted pump beam in the backward SRS generation. Inthe Raman half resonator using a dichromatic focusing lens, the conversion efficiency of SRS was more than 37% for a input pump laser energy of 40 mJ.

• The Bulletin of The Korean Astronomical Society
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• v.40 no.2
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• pp.48.1-48.1
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• 2015

Active galactic nuclei (AGNs) are powered by a supermassive black hole with an accretion disk and exhibit prominent broad and narrow emission lines. The unification model AGNs requires the presence of a geometrically and optically thick torus component that hides the broad line region from observers lying in the equatorial direction. The strong far UV radiation characterizing AGN spectra is expected to be scattered inelastically in the torus region to reappear around hydrogen Balmer lines or Paschen lines in the form of broad wings. Adopting a Monte Carlo technique we produce broad wings around $H{\alpha}$, $H{\beta}$ and $Pa{\alpha}$ that are formed through Raman scattering. The widths of the wings are mainly affected by the neutral column density of the torus, and the overall strengths are primarily determined by the covering factor and the column density of the neutral region. It is concluded that deep spectroscopy of AGNs of broad wings around hydrogen emission lines may shed much light on the AGN unification model.

• Journal of Magnetics
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• v.19 no.4
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• pp.333-339
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• 2014

Single crystal growth of $BaFe_{12}O_{19}$ by the solid state crystal growth method was attempted. Seed crystals of ${\alpha}-Fe_2O_3$ were pressed into pellets of $BaFe_{12}O_{19}$ + 2 wt% $BaCO_3$ and heat-treated at temperatures between $1150^{\circ}C$ and $1250^{\circ}C$ for up to 100 hours. Instead of single crystal growth taking place on the seed crystal, BaO diffused into the seed crystal and reacted with it to form a polycrystalline reaction layer of $BaFe_{12}O_{19}$. The microstructure, chemical composition and structure of the reaction layer were studied using scanning electron microscopy-energy dispersive spectroscopy (SEM-EDS), x-ray Diffraction (XRD) and micro-Raman scattering and confirmed to be that of $BaFe_{12}O_{19}$. XRD showed that the reaction layer shows a strong degree of orientation in the (h00)/(hk0) planes in the sample sintered at $1200^{\circ}C$. $BaFe_{12}O_{19}$ layers with a degree of orientation in the (hk0) planes could also be grown by heat-treating an ${\alpha}-Fe_2O_3$ seed crystal buried in $BaCO_3$ powder.

• Bulletin of the Korean Chemical Society
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• v.28 no.8
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• pp.1405-1409
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• 2007

The potential-induced charge transfer of the dye (Bu4N)2[Ru(dcbpyH)2-(NCS)2] (N719) on Au, Ag, and Cu electrode surfaces has been examined by surface-enhanced Raman scattering (SERS) in the applied voltage range between 0.0 and ?0.8 V. N719 is assumed to have a relatively perpendicular geometry with its bipyridine ring on the metal surfaces. A strong appearance of the carboxylate band at ~1370 cm-1 indicates that the carboxyl group will likely be deprotonated on the metal surfaces. As the electric potential is shifted from ?0.8 to 0.0 V, the ν (NCS) band at ~2100 cm-1 on the electrode surfaces appears to undergo a shift in frequency and intensity change. This indicated that the charge transfer between the dye and metal electrode surfaces had occurred. Electric-field-dependent charge transfer differs somewhat depending on the type of metal surfaces as suggested from the dissimilar frequency positions of the ν (NCS) band.

• Korean Journal of Optics and Photonics
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• v.8 no.5
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• pp.372-376
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• 1997

We study the time resolved anti-Stokes Raman spectroscopy with correlated pump and Stokes waves. When only two pump waves with relative delay are incident into a Raman medium, the Stokes waves generated by stimulated Raman scattering couple with the pump waves to generate anti-Stokes signal. Since the correlation between Stokes and the pump waves are not perfect and not quantified yet, we make a simple model fot it and calculate the normalized anti-Stokes signal intensities as a function of time delay. The broadband light regarded as chaotic field.