• Archives of Pharmacal Research
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• 제28권11호
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• pp.1270-1274
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• 2005

The vascular endothelial growth factor (VEGF) plays a key role in angiogenesis, which is a process where new blood vessels develop from the endothelium of a pre-existing vasculature. VEGF exerts its activity by binding to its receptor tyrosine kinase, KDR/Flk-1, which is expressed on the surface of endothelial cells. A methanol extract and organic solvent (n-hexane, ethyl acetate, n-butanol, aqueous) fractions from Rubus coreanus were examined for their inhibitory effects on VEGF binding to the VEGF receptor. The methanol extract from the crude drug were found to significantly inhibit VEGF binding to the VEGF receptor ($IC_{50}$$\thickapprox$27 $\mu$g/mL). Among the fractions examined, the aqueous fraction from the medicinal plant showed potent inhibitory effects against the binding of KDR/Flk-1-Fc to immobilized $VEGF_{165}$ in a dose­dependent manner ($IC_{50}$$\thickapprox$11 $\mu$g/mL). Sanguiin H-6 was isolated as an active principle from the aqueous fraction, and inhibited the binding of KDR/Flk-1-Fc to immobilized $VEGF_{165}$ in a dose­dependent manner ($IC_{50}$$\thickapprox$0.3 $\mu$g/mL). In addition, sanguiin H-6 efficiently blocked the VEGF­induced HUVEC proliferation in a dose-dependent manner ($IC_{50}$$\thickapprox$7.4 $\mu$g/mL) but had no effect on the growth of HT1080 human fibrosarcoma cells. This suggests that sanguiin H-6 might be a potential anti-angiogenic agent.

• 한국균학회소식:학술대회논문집
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• 한국균학회 2016년도 춘계학술대회 및 임시총회
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• pp.39-39
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• 2016

There is a growing consumer preference for self-medication, which has resulted in the growth of the Korean functional food market to \1.5 trillion in 2014. Functional foods that can modulate immune responses and enhance liver health are in the top 2 product-specific health functional food categories. The aim of this project was to develop and commercialize new health functional foods incorporated with Cordyceps militaris. Cordyceps genus includes about 400 species, many of which have been used as traditional medicines for many years in Asian countries. C. militaris belongs to the class Ascomycetes and has been used extensively as a crude drug and tonic food in East Asia. Owing to the various physiological activities of its main active constituent, cordyceptin, C. militaris is currently being used for multiple medicinal purposes. Recently, many studies have tried to elucidate the pharmacological mechanisms underlying the activities of Cordyceps spp., which include immune activation, anti-inflammatory, anticancer, and antiviral effects. After continuous attempts and research toward industrialization, C. militaris cultivated using brown rice was developed into a product by a standardized process and mass-cultivating system. It was successfully introduced into the market and was approved as a functional food ingredient for the first time in Korea. Based on this information, C. militaris containing functional food product for strengthening the immune system was released in August 2014 under the brand name "Dongchoong Ilgi." Dongchoong Ilgi is potentially beneficial for improving immune and liver functions and may enhance both the convenience and effectiveness of health functional foods taken by healthy people and patients with minor illness. In addition, the results of our study may be applicable for the development of health functional foods that could lower the risk of diseases such as the common cold and cancer.

• 생약학회지
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• 제38권2호통권149호
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• pp.133-138
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• 2007

Regarding chemical changes in oriental drugs after food processing such as roasting, fermentation, and extrusion, fifty commonly-used medicinal plants were investigated. As a result, Aurantii nobilis Pericarpium (a tangerine peel from Citrus unshu Markovich) showed remarkably different HPLC profiles after being roasted. An increased peak was isolated by repeated chromatography and identified as 5-hydroxymethyl furfral (5-HMF) by means of instrumental analyses. The 5-HMF content of Aurantii nobilis Pericarpoum reached its maximum level after being roasted for 30 min at 225$^{\circ}C$ (49.2 mg/g extract, ca 42 times of increase over untreated control). Although there were no significant changes in in vitro biological activity such as antioxidative, anti-dementia, anti-hypertension, anti-coagulation, or cytotoxicity, before and after roasting process, our results suggested that simple heat treatment might improve the value of the above oriental drug since 5-HMF has been known to possess inhibitory activities toward nitric oxide formation, tyrosinase, and sickling of red blood cells.

• 생약학회지
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• 제18권3호
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• pp.196-201
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• 1987

A study was carried out to investigate the possibility of acclimation of Coptis japonica Makino in Korea. Coptis japonica could be grown within 4 years as a medicinal crude drug if it is cultivated in the area of mountain facing north(300m above sea level) with 70% light interception and appropriate soil texture. It is to be noted that the propagation of the plant is more important than the yield of berberine because the berberine contents do not change in different conditions. The results were as follows; 1. The germination rates of the seeds by covering with the pine tree leaves were the highest and averaged 63%. 2. The germination rates of dry seeds were not affected in spite of low temperature and $GA_3$ treatment. 3. The germination rates of the seeds stored in moistured sand were averaged 85%, but in dry state, they were not germinated. 4. The number of leaves and fresh weight of Coptis japonica from the area of mountain facing north were increased significantly. 5. The growth and fresh weight of Coptis japonica were reduced in clay. 6. The best growth was obtained under 70% light interception. It is possible to intercrop between evergreen trees. 7. The foliage dressing did not affect the growth. 8. The number of leaves and fresh weight were increased from the 3rd year, and the average weight of rhizomes was 5.7g in 4th year. 9. The berberine contents of the rhizomes of Coptis japonica under various treatment conditions ranged from 9 to 10%. Coptidis Rhizoma from this study and from Korean market showed much higher berberine contents than those from Japanese market.

• 한국약용작물학회지
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• 제7권2호
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• pp.83-88
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• 1999

배초향 국내 수집종의 생육특성 및 수확시기별 로즈마린산과 정유함량을 조사하여 다음과 같은 결과를 얻었다. 1. 수집종들의 개화기는 8월 13일${\sim}$8월25일로 진도 수집종이 가장 늦었고 초장은 $120{\sim}170cm$, 주당 분지수는 $13{\sim}21$개, 화방군(花房群)의 길이는 $10{\sim}15cm$였으며, 화방군(花房群)의 수(數)는 $13{\sim}22$개로 수집종간에 유의성이 없었다. 2. 수집종들의 부위별 건물수량 (kg/10a)은 꽃 $109{\sim}156kg$, 잎 $135{\sim}252kg$, 줄기 $421{\sim}932kg$, 뿌리$172{\sim}394kg$으로 꽃과 뿌리에서는 유의성이 없었으며 건물수량은 개화 중기 (9월 20일)에 수확한 것이 가장 많았다. 3. 수집종들의 로즈마린산 함량은 모든 부위에서 유의성이 인정되었는데 꽃 $0.32{\sim}2.34%$, 잎 $0.97{\sim}1.64%$, 줄기 $0.03{\sim}0.21%$, 뿌리 $0.91{\sim}6.56%$로 뿌리의 함량이 가장 높고 줄기의 함량이 가장 낮았다. 4. 잎의 로즈마린산과 정유함량은 개화전(開花前)이 각각 3.76%, 5.78%로 개화후(開花後)의 1.55%, 2.10%보다 더 높았으며 지상부의 로즈마린산 함량은 개화전에 가장 높았으나 정유 함량은 개화말기에 가장 높았다.

• 한국식품영양과학회지
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• 제33권3호
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• pp.487-493
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• 2004

동백나무의 생엽과 생화, 그리고 이를 재료로 제다한 단일 동백엽차$.$화차 및 혼합 동백차의 세포독성과 다제 내성 극복을 규명하기 위하여, 4종의 인간암세포(MCF-7, Calu-5, SNU-601, AML-2/WT)와 1종의 다제내성 세포주(AML-2/ D100)를 이용하여 MTT방법으로 분석하였다. 동백의 어린엽 추출물은 Calu-6($IC_{50}$/: 79.8 $\mu\textrm{g}$/mL), SNU-601($IC_{50}$/: 39.0 $\mu\textrm{g}$/mL)에 대해 성엽과 꽃에 비해 세포독성 효과가 높았다. 단일 동백엽 차중, 찐차는 덜음차나 차차에 비해 상당한 생육 억제효과를 보였으며, 혼합 동백 차의 경우, Cahemix보다는 Catemix추출물이 MCF-7, Calu-6에 대한 $IC_{50}$/: 100 $\mu\textrm{g}$/mL 이하의 낮은 농도에서 세포 증식 억제 및 사멸 효과가 현저히 상승됨을 보여주었다. 특히 Catemix-2를 첨가하였을 때 MCF-7과 Calu-6 암세포에서 $IC_{50}$/값이 각각 75.3과 74.6으로 높은 억제효과를 나타내었다. 시료에 대하여 감수성 세포인 AML-2/WT에 대하여 세포독성을 측정하여 감수성 세포에 비하여 내성 세포에 대하여 선택적 세포독성 여부를 관찰한 결과, 대체적으로 $IC_{50}$/의 차이가 없거나 내성세포의 $IC_{50}$/이 높게 나타내어 그다지 선택적 세포독성을 나타내지 않는 것으로 보인다. 혼합 동백차의 경우, 내성세포에 대한 선택적 세포독성은 Catemix-2(녹차와 동백엽의 덜음차의 혼합)와 Catemix-3(녹차와 동백화차의 혼합)에서 0.9로 다소 효과를 보였으며, 내성 극복 효과는 Cahemix-1(CR: 1.7)에서 높게 관찰되었다.