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Fermentation and Microbial Characteristics of Korean Traditional Fermented Milk, Tarak (시판 막걸리로 제조한 전통발효유 타락의 발효 특성)

  • Jung, Jin-Kyoung;Ko, Seong-Hee;Oh, Se-Wook;Lim, Ji-Young;Chun, Tae-Hoon;Kim, SooA;Myoung, Kil-Sun;Jang, Sung Seek;Huh, Chul-Sung;Han, Young-Sook
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.44 no.4
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    • pp.602-609
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    • 2015
  • In this study, for modernization of Korean traditional fermented milk, Tarak was made using four kinds of commercial Makgeolli based on the ancient cookbook Suwoonjabbang. Samples of Tarak were periodically collected during 24 h of fermentation at $37^{\circ}C$. After fermentation, changes in pH, titration acidity, and viscosity were analyzed. Fermentation metabolites, including organic acids and free sugars, were analyzed by HPLC. Numbers of yeast and lactic acid bacteria during 24 h of fermentation were measured. The pH of Tarak significantly decreased (P<0.01), whereas its acidity significantly increased (P<0.01) during fermentation. The viscosity increased during 8~24 h of fermentation until curd was separated in Tarak. The level of ethanol increased from 0.37~0.52 mg/mL to 0.51~0.71 mg/mL during 24 h of fermentation. Lactic acid and lactose were the major organic acid and free sugar in Tarak, respectively. The number of lactic acid bacteria increased from 5.23~6.25 log CFU/mL to 9.87~10.41 log CFU/mL at the beginning during 24 h of fermentation. The number of yeast increased from 5.14~6.47 log CFU/mL to 6.99~7.73 at the beginning during 24 h of fermentation at $37^{\circ}C$. The major strains of Tarak were Pediococcus acidilactici, Lactobacillus fermentun, Lactobacillus curvatus, and Saccharomyces cerevisiae. Therefore, we concluded that Tarak was a fermented milk by both lactic acid bacteria and yeast, which was similar to koumiss or kefir.

Physicochemical Characteristics of Liquid Fertilizer made from Pig Manure in Korea (국내 돼지분뇨의 액비성분 특성 비교조사)

  • Jeon, Sang-Joon;Kim, Soo-Ryang;Rho, Kyung-Sang;Choi, Dong-Yoon;Kim, Dong-kyun;Lee, Myung-Gyu
    • Journal of Animal Environmental Science
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    • v.18 no.3
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    • pp.221-228
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    • 2012
  • Physicochemical properties of liquid fertilizer samples of resource organization, which are domestically produced and distributed, are analyzed. Major contents of the research results are as follows. 1. The ratio of complete decomposition for liquid fertilizer is 49% at Public Resource Center and 33% at Liquid Fertilizer Supply Center. The combined ratio of both half-decomposed and un-decomposed liquid fertilizers is over 50% at both centers. 2. The ratio of complete decomposed liquid fertilizer, 67%, is the highest in Gangwon and Gyeonggi-do area. The ratio of un-decomposed liquid fertilizer is high in Chungbuk and Chungnam area. The sum of ratios of the half- and un-decomposed is over 60% in the areas except Gyeonnggi-do and Gangwon-do. 3. As a result of regional comparison of the physicochemical properties of liquid fertilizers, concentration variation in most of the items are large, and the degree of uniformity is found to be considerably low. In particular, concentration variation in T-N and $NH_4$-N is the most noticeable. 4. The items that physicochemically correlated to the degree of decomposition of liquid fertilizer are appeared to be T-N, $NH_4$-N, $NO_3$-N, EC, $SCOD_{Mn}$, and ORP. 5. The physicochemical average values of the liquid fertilizer estimated as "complete decomposed" are appeared to be T-N 829 mg/L,$NH_4$-N 517 mg/L, $NO_3$-N 151 mg/L, $SCOD_{Mn}$ 1,205 mg/L, EC 10.32 mS/cm, ORP -117.12 mV.

The Perception and Emotional Experiences of Rare and Intractable Diseases in Caregivers and Pediatric Patients with Mitochondrial diseases (미토콘드리아 질환 소아 환자 보호자에서의 질환 인식 및 정서변화)

  • Eom, Soyong;Lee, Joo Young;Hyun, Jiah;Lee, Young-Mock
    • Journal of The Korean Society of Inherited Metabolic disease
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    • v.17 no.1
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    • pp.1-10
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    • 2017
  • Purpose: This study aimed to investigate the perception and emotional experiences in rare and intractable diseases for caregivers of pediatric patients with mitochondrial diseases in order to provide therapeutic interventions for patients, caregivers, and families. Methods: A total of 83 caregivers of pediatric patients with mitochondrial diseases were recruited from the pediatric mitochondrial disease clinics of the Gangnam Severance Hospital in South Korea. Participants completed the survey about their perception of mitochondrial disease and emotional experiences after the diagnosis, and these clinical data were analyzed accordingly. Results: Surveys from a total of 83 caregivers of patients were analyzed, and the patients' age ranged from 6 to 12 years (33%), followed by ages 1 to 6 years (30%). Children with mitochondrial diseases were between 0 and 0.5 years of age at the time of first symptom onset (43%), and the duration of illness lasted more than 10 years in most cases (42%). Prior to diagnosis of mitochondrial diseases, the amount of awareness the caregivers had was 'Not at all' for both rare and intractable diseases and mitochondrial diseases in 44 cases and 68 cases, respectively. For the caregivers' emotional experiences, the most common initial responses were 'Discouraged/despair', 'Helpless/lethargic', and 'Disconcerted'. 'Anxious', 'Committed to treatment', and 'Responsibility as family members' were the most common emotional responses from the caregivers, followed by 'Disconcerted' and 'Helpless/lethargic'. Conclusion: It is important to consider the level of perception and emotional experiences of caregivers and patients with rare and intractable mitochondrial diseases for planning treatment programs.

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Research of z-axis geometric dose efficiency in multi-detector computed tomography (MDCT 장치의 z-축 기하학적 선량효율에 관한 연구)

  • Kim, You-Hyun;Kim, Moon-Chan
    • Journal of radiological science and technology
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    • v.29 no.3
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    • pp.167-175
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    • 2006
  • With the recent prevalence of helical CT and multi-slice CT, which deliver higher radiation dose than conventional CT due to overbeaming effect in X-ray exposure and interpolation technique in image reconstruction. Although multi-detector and helical CT scanner provide a variety of opportunities for patient dose reduction, the potential risk for high radiation levels in CT examination can't be overemphasized in spite of acquiring more diagnostic information. So much more concerns is necessary about dose characteristics of CT scanner, especially dose efficient design as well as dose modulation software, because dose efficiency built into the scanner's design is probably the most important aspect of successful low dose clinical performance. This study was conducted to evaluate z-axis geometric dose efficiency in single detector CT and each level multi-detector CT, as well as to compare z-axis dose efficiency with change of technical scan parameters such as focal spot size of tube, beam collimation, detector combination, scan mode, pitch size, slice width and interval. The results obtained were as follows ; 1. SDCT was most highest and 4 MDCT was most lowest in z-axis geometric dose efficiency among SDCT, 4, 8, 16, 64 slice MDCT made by GE manufacture. 2. Small focal spot was 0.67-13.62% higher than large focal spot in z-axis geometric dose efficiency at MDCT. 3. Large beam collimation was 3.13-51.52% higher than small beam collimation in z-axis geometric dose efficiency at MDCT. 4. Z-axis geometric dose efficiency was same at 4 slice MDCT in all condition and 8 slice MDCT of large beam collimation with change of detector combination, but was changed irregularly at 8 slice MDCT of small beam collimation and 16 slice MDCT in all condition with change of detector combination. 5. There was no significant difference for z-axis geometric dose efficiency between conventional scan and helical scan, and with change of pitch factor, as well as change of slice width or interval for image reconstruction. As a conclusion, for reduction of patient radiation dose delivered from CT examination we are particularly concerned with dose efficiency of equipment and have to select proper scanning parameters which increase z-axis geometric dose efficiency within the range of preserving optimum clinical information in MDCT examination.

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Spatial Variation in the Reproductive Effort of Mania Clam Ruditapes philippinarum during Spawning and Effects of the Protozoan Parasite Perkinsus olseni Infection on the Reproductive Effort (여름철 산란기에 있어 바지락 번식량의 공간적 변이와 기생 원생생물 Perkinsus olseni 감염이 바지락 번식에 미치는 영향)

  • Kang, Hyun-Sil;Hong, Hyun-Ki;Yang, Hyun-Sung;Park, Kyung-Il;Lee, Taek-Kyun;Kim, Young-Ok;Choi, Kwang-Sik
    • Ocean and Polar Research
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    • v.37 no.1
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    • pp.49-59
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    • 2015
  • Spatial variation in the reproductive effort of Manila clam Ruditapes philippinarum is often closely associated with variation in the seawater temperature and food availability, which determines gonad maturity and the quantity of gamates produced during spawning. Previous studies also have reported that severe infection by the protozoan parasite Perkinsus olseni exerts a negative impact on clam reproduction, retarding gonad maturation or decreasing the reproductive effort. In the present study, we investigated impacts of P. olseni infection on the reproductive condition of Manila clam during a spawning season. Histology revealed that 54% of female clams in Wando off the south coast were in spawning, while only 10% of the female from Gomso and 0% of the female from Seonjaedo in Gyeonggi bay off the west coast were engaged in spawning at the end of May in 2004. Ray's fluid thioglycollate media (RFTM) assay was applied to assess P. olseni infection and indicated that the infection intensity in Wando ($3,608,000{\pm}258,000cells/g$ wet tissue) was significantly higher than the levels in Gomso ($1,305,000{\pm}106,000cells/g$ wet tissue) and Seonjaedo ($1,083,000{\pm}137,000cells/g$ wet tissue, p < 0.001). The size of the ripe female follicle determined from histology was significantly smaller in Wando ($0.032mm^2$) compared to the sizes in Gomso ($0.059mm^2$) and Seonjaedo ($0.052mm^2$, p < 0.05). Accordingly, the number of ripe eggs in the follicle was significantly fewer among clams in Wando (14) compared to the numbers determined in Gomso (23) and Seonjaedo (22). The absolute quantity of egg in ripe clams from Wando (31.01 mg) was also significantly smaller than Seonjaedo (61.79 mg) and Gomso (133.3 mg). Quantity of total protein, carbohydrate, and lipid in the tissue in the Wando samples was significantly smaller than the quantities determined in Gomso and Seonjaedo (p < 0.001). The observed poor reproductive condition and proximate tissue composition of the females in Wando were, in part, explained by the extremely high level of the parasites, sapping the ability to store energy in the host tissues, which is used in tissue growth and the egg production.

Morphological and Morphophysiological Dormancy in Seeds of Two Genera (Heloniopsis and Thalictrum) Native to Korea (자생 관상용 Heloniopsis와 Thalictrum속 종자의 형태적 및 형태생리적 휴면)

  • Lee, Seung Youn;Rhie, Yong Ha;Jung, Hyun Hwan;Kim, Ki Sun
    • Horticultural Science & Technology
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    • v.32 no.3
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    • pp.310-317
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    • 2014
  • This study was conducted to find basic information for the research of morphological dormancy in seeds of two genera (Heloniopsis and Thalictrum) native to Korea. Seeds of Heloniopsis koreana, Heloniopsis tubiflora, Thalictrum rochenbrunianum, Thalictrum uchiyamai, and Thalictrum coreanum were collected, and embryo morphology and seed germination were investigated. All seeds of five species had underdeveloped embryos which occupied about 10-20% of the full seed length at maturity. The seeds of H. koreana and H. tubiflora had rudimentary embryos. The seeds of T. rochenbrunianum, T. uchiyamai, and T. coreanum had intermediate type between rudimentary and linear embryo. The small embryos of all seeds of five species grew to a critical length before radicles emerge from the seeds. At 30 days after sowing, the seeds of H. koreana and H. tubiflora germinated to 22.7% and 40.7%, respectively. On the other hand, the seeds of T. rochenbrunianum, T. uchiyamai, and T. coreanum germinated to 12.5%, 3.3%, and 0%, respectively. If embryo growth and germination in seeds are completed in approximately 30 days, the seed are said to have morphological dormancy (MD). However, if the seeds require > 30 days for germination, they are considered to have morphophysiological dormancy (MPD). Thus, the seeds of H. koreana and H. tubiflora had about 78% and 60% MPD, respectively. On the other hand, the seeds of T. rochenbrunianum, T. uchiyamai, amd T. coreanum had about 87%, 96%, and 100% MPD, respectively. There was a different level of dormancy (MD and MPD) within the same seed population examined. Therefore, germination of the seeds was delayed due to the underdeveloped embryos. This result could contribute to determine morphological seed dormancy and germination mechanism in seeds of the Korean Peninsula.

Studies on the Chemical Compositions and Quality of Red Pepper Paste Brewed with Different Raw Materials (담금원료(原料)에 따른 고추장의 성분(成分)과 품질(品質)에 관(關)한 연구(硏究))

  • Park, Soo-Woong;Park, Yoon-Joong
    • Korean Journal of Agricultural Science
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    • v.6 no.2
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    • pp.205-212
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    • 1979
  • Red pepper paste were prepared by using various raw materials such as rice, glutinous rice, polished barley, polished wheat and corn powder, and their chemical compositions including reducing sugar, amino-N and ethanol were analyzed during the period of aging in order to elucidate effects of these starchy materials on the compositions and qualities of the products. The results obtained are summarized as follows: 1) Koji of these materials were manufactured respectively by inoculating a strain of Asporyzae and their activities of protease and saccharifying amylase were determined. Wheat koji was found to have the highest level of protease activity among the koji, while glutinous rice koji had the strongest amylase activity. 2) Contents of moisture, crude protein, crude fat, and sodium chloride in the red pepper pastes were not changed significantly, however total sugar content was decreased during the period of aging. 3) After 60 days of aging, the highest amount (160mg%) amino nitrogen was detected in the red pepper paste of polished wheat, but higher levels of reducing sugar and ethanol contents were detected in that of glutinous rice. 4) Amino acids in the products were analyzed after 60 days of aging: a) Total 17 amino acids were detected. b) Free amino acid ratio to total amino acid content was approximately 35 percent in average. c) Free glutamic acid was higher than any other free amino acid In the amount and free ratio, but free methionine was lower. d) Lysine content was relatively high, while histidine and glycine were detected in trace. 5) Sensory tests including color, odor and taste on the products resulted in the highest mark for the red pepper paste of glutinous rice but in the lowest mark for that of corn powder.

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Genotype and Environment Influence on Raffinose and Stachyose Content of Soybean Seed (콩 종자의 Raffinose 및 Stachyose 함량에 대한 유전자형과 환경의 영향)

  • Sung, Mi Kyung;Han, Sung Jin;Seo, Hyung Jin;Choi, Sang Woo;Nam, Sang Hae;Chung, Jong Il
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.59 no.3
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    • pp.319-324
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    • 2014
  • Soybean (Glycine max (L.) Merr.) is an important crop for protein, oil, carbohydrates, isoflavones, and many other nutrients to humans and animals. But, antinutritional factors in the raw mature soybean are exist. Raffinose and stachyose are main antinutritional factors in soybean seed. Both raffinose and stachyose are carbohydrates, belonging to the raffinose family of oligosaccharides (RFOs). RFOs are not readily digested in humans and cause flatulence or diarrhea. The objective of this research is to obtain the information on raffinose and stachyose content according to genotype and environment. A total of twenty two soybean genotypes (11 cultivars, 3 germplasms and 8 breeding lines) were selected. Each genotype was grown in the field for two years with two replications and harvested in bulk at natural maturity for two years. Content of raffinose and stachyose was detected by HPLC. The raffinose content (g/kg) of 22 genotypes was $2.68{\pm}0.21-5.87{\pm}2.43$ in year 1 and was $3.24{\pm}0.37-9.05{\pm}0.16$ in year 2. The stachyose content (g/kg) was $4.23{\pm}0.98-27.68{\pm}9.90$ at year 1 and was $5.11{\pm}1.09-25.32{\pm}0.35$ in year 2. Genotype and environment have highly significant effects on raffinose and stachyose content. Three genotypes (Da-7, 116-13, and RS-78) have low stachyose content at 5% significant level in two years. A positive correlation ($R^2=0.1985^*$) between raffinose and stachyose was observed in year 2. These informations are valuable in soybean genetics and breeding program related with raffinose and stachyose content.

Influence of Dissolved Ions on Geochemical Dissolution of Uranium in KURT Granite (KURT 화강암 내 우라늄의 지화학적 용출특성에 미치는 용존이온의 영향)

  • Cho, Wan Hyoung;Baik, Min Hoon;Ryu, Ji-Hun;Lee, Jae Kwang
    • Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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    • v.16 no.3
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    • pp.281-290
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    • 2018
  • In order to understand the long-term behavior of radionuclides in granite environments, geochemical behavior characteristics of uranium in granitic host rock of KURT (KAERI Underground Research Tunnel) were investigated by dissolution experiment with different reaction time and solutions. In the dissolution experiment, significantly increased dissolution levels of uranium from granite powder samples were identified during the reaction time of 0~10 days for reaction solutions ($UD-CO_3$ and UD-Bg) containing a large amount of $CO_3{^{2-}}$. On the other hand, significantly increased dissolution levels of uranium were also identified for reaction solutions containing Na and Ca after 60 days. Dissolution of uranium continuously increased in reaction solutions of $UD-CO_3$ ($44.61{\mu}g{\cdot}L^{-1}$), UD-Bg ($41.01{\mu}g{\cdot}L^{-1}$), UD-Na ($26.87{\mu}g{\cdot}L^{-1}$), UD-Ca ($20.26{\mu}g{\cdot}L^{-1}$), UD-CaSi ($17.03{\mu}g{\cdot}L^{-1}$), and UD-Si ($10.47{\mu}g{\cdot}L^{-1}$) in the experimental period of ~270 days. However, after day 270, dissolution of uranium showed a decreasing tendency. This is thought to have occurred because existing uranium in granite samples reached the limit of dissolution by interaction with reaction solutions. Concentrations of dissolved uranium and points of maximum concentration value were found to differ depending on the $CO_3{^{2-}}$ presence in the mixed reaction solution and on the geochemical type of the water. It is estimated that differences in the reaction rate between the granite sample and the reaction solution are due to the influence of dissolved ions in the reaction solution.

A Study on the Consumptive use of Irrigated Water in Paddy Rice (벼의 품종별(品種別) 필요수량(必要水量) 관(關)한 실험분석(實驗分析))

  • Suh, Seung Duk;Choi, Won Chil
    • Current Research on Agriculture and Life Sciences
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    • v.3
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    • pp.36-43
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    • 1985
  • This study was conducted to find out the consumptive use of irrigated water for calculation of a duty of water in paddy rice. Tall statured Japonica rice variety is Jinju(late). Short statured Tongil varities-Taebaek(early) and Pungsan(medium) were planted on the experimental farm of kyungpook National University in 1983. The results obtained in this study were as follows : 1. The average temperature of the underwater surface was $27.1^{\circ}C$ and the maximum was recorded at beginning of the August. 2. The evapotranspiration was gradually increased after transplanting and showed the peak from booting to heading stage of rice varieties. The average evapotranspiration through the whole growing period was 8. 43mm/day(Jinju) for tall statured Japonica variety, and 7.78mm/day(Taebaek), 7.71mm/day(Pungsan) for short statured Tongil varieties. 3. The ratio of evapotranspiration to pan-evaporation through the whole growing period was 1.71 for Japonica variety and 1.55 for Tongil varieties. 4. K-value in Blaney & Criddle formula was 1.41 for Japonica variety and 1.30 for Tongil varieties. Coefficient consumptive use of water (Kc-value) was 1.40 for Japonica variety and 1.27 for Tongil varieties. 5. It appeared that the water consumption in the 6, 8 cm pot is more than that of 2, 4 cm pot but the number of panicle of the 6, 8 cm pot is somewhat less than that of 2, 4 cm pot. The deeper water level is the less tiller became. It is consindered that 2 cm pot is more profitable than the others.

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