• Title/Summary/Keyword: Zusanli acupoint

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Acupuncture Suppresses Intrastriatal Hemorrhage-Induced Neuronal Cell Death and Proliferation in Rats

  • Cho, Nam-Hun;Park, Dong-suk;Lee, Jae-Dong
    • The Journal of Korean Medicine
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    • v.24 no.4
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    • pp.127-135
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    • 2003
  • Objectives : Intracerebral hemorrhage (ICH) is one of the most devastating types of stroke. The effect of acupuncture on the intrastriatal hemorrhage-induced neuronal cell death and cell proliferation in rats is examined. Methods : Cell death and cell proliferation in rats was investigated via terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay and immunohistochemistry for caspase-3 and 5-bromo-2'-deoxyuridine (BrdU). Results : Results showed that apoptotic cell death in the striatum and cell proliferation in the hippocampal dentate gyrus significantly increased following intrastriatal hemorrhage in rats, and that acupunctural treatment at the Zusanli acupoint suppressed the hemorrhage-induced increase in apoptosis in the striatum and cell proliferation in the dentate gyrus. Conclusions : It is suggested that acupunctural treatment, especially at the Zusanli acupoint, may aid recovery following central nervous system sequelae following ICH.

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The Trend of Experimental Study on the Effect of Acupuncture at $ST_{36}$(Zusanli) (족삼리(足三里) 자침(刺鐵)이 생체(生體)(생체)에 미치는 영향(影響)에 대한 실험연구동향 (PubMed 검색을 통한 문헌고찰))

  • Kim, Yoon-Hee;Yim, Yun-Kyoung;Lee, Hyun
    • Korean Journal of Acupuncture
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    • v.22 no.1
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    • pp.133-150
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    • 2005
  • Objective : The purpose of this study is to examine the tendency of experimental studies on the physiological effects of acupuncture at $ST_{36}$(Zusanli). Methods : We investigated 69 theses (10 Korean and 59 international) which were searched by the keyword 'Zusanli' through PubMed website, and that were experimented with manual acupuncture or electroacupuncture on healthy human subjects or normal animals. Results : The 69 theses were classified into iou groups based on the main topic which is related with the influence of acupuncture stimulation at $ST_{36}$(Zusanli), such as digestive system, nervous system, immune system, and cardiovascular system. The main results found in this study are : 1. Acupuncture at $ST_{36}$(Zusanli) increases gastric mobility. And such effect is related with vagal nerve and opioid pathway. 2. Acupuncture at $ST_{36}$(Zusanli) is valuably related with cerebral cortex. And it influences on the cerebrum activities. 3. Acupuncture at $ST_{36}$(Zusanli) has noticeable analgesic effect, which is related with Opioid mechanism, Gate control and SP 4. Acupuncture at $ST_{36}$(Zusanli) increases immunity Conclusion . Acupuncture at $ST_{36}$(Zusanli) has many effects on digestive system, nervous system, immune system, and cardiovascular system.

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Effects of Zusanli (ST36) Acupuncture on Abdominal Thermography of Health Subject (족삼리(足三里) (ST36) 자침(刺鍼)이 복부(腹部) 한열변화(寒熱變化)에 미치는 영향(影響))

  • Sim Won-Bo;Kim Jae-Hyo;Kim Jong-Sung;Song Jae-Soo;Kim Kyung-Sik;Sohn In-Cheul
    • Korean Journal of Acupuncture
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    • v.20 no.1
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    • pp.1-20
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    • 2003
  • This study was examined for effects of acupuncture of Zusanli (ST36) on the abdominal thermography of health subject. The volunteers who participating in this study had taken rest for 20-30 mins in room temperature (23-$25^{\circ}C$) before the examination and informed them what to prohibit smoking, drinking and administration of drug for the previous day. The thermography of abdomen including a below part of the chest was taken using Infra-Red Imaging System (IR 2000, MEDI-CORE Co, Korea) by time interval of 15 minutes at 15 min before, just before and 15 min after, 30 min after and 45 min after acupuncture stimulation. Acupuncture was applied to the left ST36 acupoint for 30 mins. The results showed that acupuncture of ST36 significantly had more potencies of changes on all the of abdominal thermography than those of control group. Also, it was observed that the quantities of thermal changes following acupuncture of ST36 been increased significantly comparing that of control group at all the ROIs (region of interest). Observed the thermography classified by ROI, however, it was failed that acupuncture of ST36 could modulate the specific areas concerning to the abdominal pathway of Stomach Meridian. These results suggest that acupuncture of ST36 may modulate thermal distributions and changes of abdominal areas including the below of chest.

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Analgesic and Anti-inflammatory Effects of Sono-acupoint Therapy (초음파경혈요법의 진통소염효과 연구)

  • Lim, Sabina;Son, Yang-sun;Jin, Soo-hee
    • Journal of Acupuncture Research
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    • v.19 no.5
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    • pp.176-188
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    • 2002
  • Objective : Sono-acupoint (SA) therapy is a new therapeutic technique that combined with acupuncture points, herbal medicine and ultrasound therapy. This study was carried out to investigate the analgesic and anti-inflammatory effects of sono-acupoint therapy. Methods : We performed the tail-flick test with normal rats to examine the tail-flick latency (TFL), and the Freund's adjuvant-induced arthritis rat model to examine the edema, skin temperature and serum concentration of c-reactive protein and rheumatoid factor (RF). Herbal SA (HSA) treatment was performed at bilateral Zusanli (ST36) with the hanbang-gel made of several selected herbal drugs in Sprague-Dawley rats (male, $250{\pm}30g$). General SA (GSA) treatment was performed at bilateral Zusanli (ST36) with the gel used in ultrasound therapy. In arthritis rat model, Freund's adjuvant (50mg/ml) was injected in dorsal part of right foot, and these treatments were performed after 15 days. Results : TFL was lengthened after SA treatments. Skin temperature and RF concentration that were the evidence of arthritis in rats were decreased by HSA treatment (P < 0.05). Conclusion : These results indicate that HSA has the analgesic and anti-inflammatory effects in rats, and further developments will produce the advance of this new therapeutic skill.

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A Literature Study on Acupuncture for Cerebral Palsy -Based on the Current Traditional Chinese Medical Journals- (뇌성마비의 침자(鍼刺) 치료에 대한 문헌적 고찰 -최근 중의잡지를 중심으로-)

  • Ha, Su-Yun;Min, Sang-Yeon;Kim, Jang-Hyun
    • The Journal of Pediatrics of Korean Medicine
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    • v.23 no.1
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    • pp.205-228
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    • 2009
  • Objectives The purpose of this study is to investigate the current acupuncture therapy of cerebral palsy. Methods We investigated the Chinese clinical papers which were published in the last 10 years(from 1999 to 2008). We found these papers from the oriental medical library in university and we also used the China National Knowledge Infrastructure(CNKI) through the internet and selected 32 papers for analyzing. Results Most papers were described the effect of acupuncture or acupoint-injection. This is more effective way to treat than the general rehabilitation treatment such as the physical therapy, the occupational therapy, and the speech therapy. Acupuncture or acupoint-injection has overall $80{\sim}100%$ of rehabilitation rate. The younger the children were, the longer the treatment period was, and the more successful in treatment. The acupuncture was often used with the general acupuncture and scalp acupuncture. Commonly used major acupuncture points were sishencong(四神聰), bohui(百會), zusanli(足三里), yundongqu(運動區), pinghengqu(平衡區), quchi(曲池), and sanyinjiao(三陰交). Commonly used main meridian pathways were bladder, governor vessel, gallbladder, large intestine, stomach, small intestine meridian. Head is the common site for acupuncture. The main acupoint-injection points were zusanli(足三里), dazhui(大椎), shenshu(腎兪), yamen(啞門), neiguan(內關), and fengchi(風池). For the injection, brain activator, ganglioside M1, cerebroprotein hydrolysate, cytidine diphosphate choline, Vit B1, Vit B12, the salviae root, the safflower were commonly used. Conclusions Acupuncture and acupoint-injection have been shown as an effective treatment on cerebral palsy. The acupuncture was used often the general acupuncture and scalp acupuncture all together. Commonly used main acupuncture points were sishencong, bohui, zusanli, yundongqu, pinghengqu, quchi, sanyinjiao.

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Effect of Acupuncture on 6-Hydroxydopamine-induced Nigrostriatal Dopaminergic Neuronal Cell Death in Rats

  • Kim, Yeung-Kee;Song, Yun-Kyung;Lim, Hyung-Ho
    • The Journal of Korean Medicine
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    • v.26 no.4
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    • pp.98-107
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    • 2005
  • Objectives: Acupuncture treatment has been clinically used for functional recovery in Parkinson's disease. In the present study, we investigated the effect of acupuncture at Zusanli (ST36) on nigrostriatal dopaminergic neuronal cell death in rats. Methods: A Parkinson's disease model was induced by the unilateral injection of 6-hydroxydopamine (6-OHDA) into the striatum. Acupuncture treatment was performed at Zusanli (ST36) and at the hip, as a non-acupoint, once a day for 14 days. Two weeks after 6-0HDA injection, an apomorphine-induced rotational behavior test showed significant rotational asymmetry in rats with Parkinson's disease. Immunostaining for tyrosine hydroxylase demonstrated a dopaminergic neuronal loss in the substantia nigra and dopaminergic fiber loss in the striatum. Results: Acupuncture at the ST36 acupoint significantly inhibited rotational asymmetry in rats with Parkinson's disease, and also protected against 6-OHDA-induced nigrostriatal dopaminergic neuronal loss. These effects of acupuncture were not observed for non-acupoint acupuncture. Conclusions: The present study shows that acupuncture treatment, especially at the ST36 acupoint, can be used as a useful strategy for the treatment of Parkinson's disease.

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Histological Changes of the Acupoint by Acupuncture Stimulation (침자(鍼刺)한 경혈(經穴) 부위(部位)의 조직학적 변화(組織學的 變化))

  • Kim, Jeong-Sang;Park, Min-Hee;Cho, Kwang-Phil;Jung, Hae-Man
    • Applied Microscopy
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    • v.30 no.1
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    • pp.81-87
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    • 2000
  • This study has been carried out to understand histochemical and ultrastructural changes by acupuncture stimulation at the acupoint of the Zusanli (St. 36) in the rat. A lot of mast cells are observed in the peripheral of the hole with a sparrow-pecking and twistin -needle manipulation to the acupoint Zusanli for induction of 'Qi'. These cells contained a lot of granules with varied electron density and exocytosis granules were observed near the mast cell. H-bands of the muscle fiber that situated near the hole were shortened. It is assumed that these muscles are contracted by acupuncture stimulation. These results imply that functional relationship between mast cells in the dermis and Qi-sensation induced by acupuncture plays an important role on the specific receptor response to the mechanical stimulation.

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Effects of Long Term Stimulated Acupuncture at ST 36 on the Serum Gastrin Level in Rats (족삼리 장기 매침이 정상 백서의 혈중 Gastrin 농도에 미치는 영향)

  • Jang Kyung Hoon;Kim Myong Dong;Yu Yun Cho
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.17 no.3
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    • pp.672-676
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    • 2003
  • To investigate the effect of acupuncture on Zusanli (ST 36) in this study, gastrin level of serum by radioimmunoassay was measured at 1, 3, 6 weeks in rats. In other groups, 1 mg/day omeprazole was administered for 1, 3, 6 weeks. Omeprazole increased significantly gastrin level of serum at 1, 3, 6 weeks in a time dependent manner. Acupuncture applied to the ST 36 acupoint decreased at 1, 3, 6 weeks. Furthermore, acupuncture applied to other acupoint, Yanglinquan (GB 34) did not produced significant effect. When the common peroneal nerve was dissected, acupuncture of ST 36 produced the change of gastrin level. These data suggest that acupuncture decreased gastrin level of serum in point specific way and that effect was not related with surrounding nerve.

Effects of GaAsAl Laser on the Pressure Pain Threshold in Rats (GaAsAl 레이저 자극이 흰쥐의 압통역치에 미치는 영향)

  • Song, Young-Wha;Lee, Young-Gu;Lim, Jong-Soo
    • Journal of Korean Physical Therapy Science
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    • v.7 no.2
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    • pp.533-543
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    • 2000
  • This study was designed to evaluate the analgesic effect of low power GaAsAl laser on the pain threshold of mechanical stimulation using different treatment points, acupuncture point (zusanli) and non-acupuncture points(back). Furthermore, we investigated the analgesic effect of low power GaAsAl laser using the different duration and intensity of laser in mechanical stimulation induced pain behavior. The results were summarized as follows: 1. The threshold of mechanical stimulation was significantly increased by GaAsAl laser stimulation into zusanli point after 15 and 30 min after laser stimulation(P<05). However, the laser stimulation into non-acupoint did not affect the pain threshold of mechanical stimulation. with dose dependent manner. 2. In order to investigate the analgesic effects of BV depending upon different intensities of laser stimulation, the experimental animals were divided into three groups: 3 mW treated group, 6 mW treated group and 10 mW treated group. The low power GaAsAl laser stimulation was applied into zusanli acupoint for 30 min with different intensity of laser stimulation. Six and ten mW of laser stimulation significantly increased the pain threshold of mechanical stimulation at 15 min after laser stimulation as compared to that of control group(P<.05). Moreover, the analgesic effect of 10 mW laser stimulation was maintained for 30 min after laser stimulation (P<.05). 3. Finally, we tested the analgesic effect of 10 mW laser stimulation using different duration such as 10 min, 30 min or 1 hr after application of mechanical stimulation. In 30 min treatment group, the pain threshold of mechanical stimulation was increased at 15min and 30min after laser stimulation(P<.05). However, laser stimulation for 60 min dramatically increased the pain threshold of mechanical stimulation at 0 min after laser stimulation and the analgesic effect of laser stimulation was observed until 1 hr after laser stimulation. In conclusion, these data apparently demonstrate that low power GaAsAl laser has analgesic effect on mechanical induced pain model in rats. In addition, the treated point, intensity and duration of laser stimulation should be concerned before clinical application for pain management purpose.

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Effect of Acupuncture on Depression and Cell Proliferation in Hippocampal Gyrus Dentatus of Maternal-separated Rat Pups (침술 치료가 모성분리 새끼 쥐의 우울증과 해마의 치상회 세포증식에 미치는 영향)

  • Park, Jung-Sik;Lim, Hyung-Ho
    • Journal of Korean Medicine Rehabilitation
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    • v.29 no.2
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    • pp.91-99
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    • 2019
  • Objectives The loss of maternal care during early postnatal period may increase development of mood-related disorders, such as depression, anxiety, and personality disorders. In this study, the effect of acupuncture on depression in relation with cell proliferation in the hippocampal gyrus dentatus was investigated using maternal-separated rat pups. Methods On the postnatal 14th day, rat pups from six dams were grouped into following groups: maternal care group, maternal separation group, maternal separation and non-acupoint-acupunctured group, maternal separation and Zusanli-acupunctured group, and maternal separation and fluoxetine-treated group. Acupuncture was performed from postnatal 28th day to postnatal 37th day. The rat pups that belong in the maternal separation and fluoxetine-treated group were injected subcutaneously with 5 mg/kg fluoxetine hydrochloride once a day for the same period of time. To evaluate activity of the rat pups, open field test was performed. Immunohistochemistry for serotonin (5-hydroxytryptamine, 5-HT) and tryptophan hydroxylase (TPH) in the dorsal raphe and for 5-bromo-2'-deoxyuridine (BrdU) in the hippocampal gyrus dentatus was conducted. Results The present results reveal that the activity was decreased by maternal separation. In contrast, acupuncture at Zusanli overcame maternal separation-induced hypoactivity. Maternal separation suppressed TPH expression and 5-HT synthesis in the dorsal raphe and decreased cell proliferation in the hippocampal gyrus dentatus of rat pups. In contrast, acupuncture at Zusanli alleviated maternal separation-induced decrease of 5-HT synthesisand TPH expression. Conclusions The present results demonstrate that acupuncture at Zusanli ameliorated depressive state through increasing cell proliferation and enhancing 5-HT synthesis.