• Culinary science and hospitality research
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• v.20 no.6
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• pp.262-274
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• 2014

In this study, changes of nutrients such as vitamins, minerals, and antioxidants were determined by using a multi-functional grinder. Contents of vitamin C and vitamin $B_1$ were measured by HPLC, and antioxidant activity was estimated toward free radical scavenging effect in using DPPH. Contents of minerals(Zn, Mn, Fe, Mg, K, and Ca) were computed by ICP-Mass. Results identified that the contents of vitamin C was $44.06{\pm}10.86mg/kg$ in mandarine, $132.1{\pm}22.80mg/kg$ in orange, and $12.79{\pm}2.01mg/kg$ in pineapple by using the hand blender for 3 minutes, and the loss rate of vitamin C contents were 12%, 7%, and 12% in comparison with the control group. In addition, the contents of vitamin C were $41.89{\pm}5.55mg/kg$ in mandarine, $131.51{\pm}12.67mg/kg$ in orange and $16.76{\pm}1.47mg/kg$ in pineapple when using of the grinder for 3 minutes, and the loss rate of vitamin C contents were 16%, 8%, and 17%. The results of vitamin $B_1$ showed a tendency to decrease in the same manner as the content of vitamin C even if there was no significant difference. Furthermore, the result of antioxidant activity revealed that free radical scavenging effect of sample was 60% decreased when using a hand blender and 10% decreased when using a grinder. Thus decrease rate of antioxidant activity when using the hand blender was higher than grinder. Lastly, current study could find any significant differences among the 16 food samples for cooking when employing the multi-functional grinder(p<0.05).

• Journal of Environmental Health Sciences
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• v.27 no.4
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• pp.63-71
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• 2001

PM 2.5/ samples were measured at two sites, Hadan(suburban site) and Anrak (roadside site) in Busan area. PM 2.5/ sampling was performed for 24-hour intervals by the FH9.5 particulate sampler. Aerosol samples were collected on PTFE filter. A total of 60 particulate samples were collected, dad samples were measured for Particulate mass concentration, metallic elements (Cr, Mn, Ni. Cu. Se, Fe, Pb, and Zn) and waer-soluble elements (C $l^{[-10]}$ , N $O_{3}$$^{[-10]}$ ,S $O_{4}$$^{2-}$, N $H_{4}$$^{+}$, $Ca_{2}$$^{+}$, $Mg_{2}$$^{+}$ and $^{+}$.Mass concentration in Hadan ranged 24.23~57.12 $\mu\textrm{g}$/㎥ and 60.22~72.12 $\mu\textrm{g}$/㎥m Yellow Sand Events. Major cations in Hadan and Anrak site is N $H_{4}$$^{+}$and N $a^{+}$ respectively. SO42$^{[-10]}$ was the abundant specie in the PM 2.5 fraction for Hadan site an dAnrak site. Hadan site showed igher concentration in S $O_{4}$$^{2.1}$ and N $H_{4}$ $^{+}$ In Anrak site the concentration of S $O_{4}$/sip 2-/and N $a^{+}$ was higher than other ions Prominent metallic elements were Fe and Pb in two sites. Principal component analysis showed that main source of PM 2.5 aerosol particles was non-metal related source which was resulted in relating elements as Cr, Ni, and Pb at Hadan site, Anrak site also has resulted PM2.5 aerosol paricles source, which was related its element like Zn, and Ni,. The SAS package analysis also showed that long-range transport effect at Hadan area due to Yellow Sand Event by the prevailing weaterlies.ling weaterlies.

• Proceedings of the Korean Institute of Surface Engineering Conference
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• 2017.05a
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• pp.153-153
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• 2017

The application of the coating supports the mechanical characteristics of the implant, and various materials and coatings are currently being used in the implant in a way to accelerate adhesion. Especially, plasma electrolytic oxidation (PEO) coating has been proposed continually with good surface treatment of titanium alloys. Also, the PEO process can incorporate Ca and P ions on the titanium surface through variables varied factor. PEO process for bioactive surface has carried out in electrolytes containing Ca and P ions. Natural bone is composed of mineral elements such as Mg, Si, Zn, Sr, and Mn, etc. Especially, Mg and Si of these elements play role in bone formation and growth after clinical implantation of bio-implants. In this study, corrosion charateristics of PEO-treated Ti-6Al-4V alloy in solution containing Si and Mg ions has been investigated using several experimental techniques. The PEO-treated surfaces were identified by X-ray diffraction, using a diffractometer (XRD, Philips X' pert PRO, Netherlands) with Cu $K{\alpha}$ radiation. The morphology was observed by field-emission scanning electron microscopy (FE-SEM, Hitachi 4800, Japan) and energy-dispersive X-ray spectroscopy (EDX, Oxford ISIS 310, England). The potentiodynamic polarization and AC impedance tests for electrochemical degradations were carried out in 0.9% NaCl solution at similar body temperature using a potentiostat with a scan rate of 1.67mV/s and potential range from -1500mV to + 2000mV.

• Clinical and Experimental Pediatrics
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• v.49 no.1
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• pp.18-23
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• 2006

Purpose : The reliability of hair mineral analyses regarding nutritional status, environmental exposure or diseases is controversial. The aim of this study was to determine the normal reference range of hair mineral concentration of Korean children. Methods : We examined hair mineral concentrations of 223 children(3-12 yrs old, 110 boys, 113 girls, mean age $8.8{\pm}2.2$ yrs old) living in the northern area of Seoul. The trace elements including six toxic elements(Al, As, Cd, Ba, Hg, Pb) and 11 nutritional elements(Na, Mg, P, K, Ca, Cr, Mn, Fe, Cu, Zn, Se) were analyzed by inductive coupled plasma mass spectrometry(ICP-MS). Results : The mean concentrations of Ca and Mg were higher in girls than in boys. The mean concentrations of Cd, Pb and Cr were higher in boys than girls. The Zn, Ca, Mg, Cu and Hg levels in hair samples were positively correlated with increasing age. The Zn levels of the Korean children's hair samples appear to be lower than that of other countries' reference values. Conclusion : There are considerable differences in hair mineral concentrations by age, sex and race. Additional research is needed to establish Korean reference values, and to evaluate the usefulness of hair mineral analyses as a screening tool for nutrition- and environment-related childhood diseases.

• Asian-Australasian Journal of Animal Sciences
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• v.24 no.11
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• pp.1595-1600
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• 2011

Sugarcane press residue (SPR), a byproduct from the sugar industry was evaluated for it's nutrient and energetic quality in broilers and layers. The composition of SPR included (% DM): CP-11.76 (methionine-2.21, cystine-1.05, lysine-4.85, threonine-5.48% of CP), EE-7.87 (palmitic acid-30.3, stearic acid-4.1, oleic aicd-17.2, linoleic acid-38.0, linolenic acid-5.4% of EE), CF-10.08, TA-21.08 (Ca-3.87, P-1.10, Mg-0.95%, Fe-3500, Mn-284, Zn-113, Cu-61.5, Co-5.0 ppm and AIA-4.93%) and NFE-48.35% indicating that SPR is a valuable source of both organic and inorganic nutrients for poultry. The metabolic trials revealed the average ME of SPR as 749, 842 and 1,270 kcal/kg, respectively in broilers and 844, 936 and 1,031 kcal/kg in layers, at 10, 20 and 30% inclusion levels, respectively. Further, the fortification of SPR incorporated diets with biotechnological products viz., lipid utilizing agents (lipase and lecithin) or NSP degrading enzymes and their combination did not improve the ME content of such diets.

• International Journal of Industrial Entomology and Biomaterials
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• v.8 no.1
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• pp.1-15
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• 2004

Due to recent scientific innovations in mulberry cultivation, leaf yield has been increased manifold. However, with successive leaf harvest, a quantum drop in leaf yield and quality has been noted. This inturn has affected the silkworm rearing and farmers suffered by the frequent crop failures. This is mainly due to nutrient deficiencies in mulberry leaf. Moreover, no complete information is available about hunger signs of nutritional disorders, analytical diagnosis and critical levels of nutrients required. The present paper, thus, may serve as an important nutrient guide for identification of hunger signs, leaf nutrients status under deficiency and critical levels of the elements namely N, P, K, Ca, Mg, S, B, Cu, Fe, Mn and Zn requirements for higher leaf yield and quality. The leaf nutrient status provided may help chemist for correcting the soil status. Besides, an integration of mulberry intercropping with legumes and applications of neem and castor oil cakes, VA-mycorrhizal inoculation, biofertilizer and vermicompost are suggested as integrated nutrient management for sustainable sericulture industry. Based on the information described in this paper, a model needs to be framed for maintaining continuous supply of nutrients to obtain desired quantity and quality of mulberry leaf for successful silkworm cocoon crop and increasing overall silk productivity.

• Journal of Korean Society for Atmospheric Environment
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• v.13 no.6
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• pp.475-486
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• 1997

The characteristics of atmospheric aerosols were investigated as a function of particle size and water solubility. The atmospheric aerosols were sampled with classifying into 12 size ranges by the use of Andersen low-pressure impactor. Collected aerosol particles were extracted by ultrapure water and filtered to be separated into water-soluble and insoluble components. The concentrations 12 elements in both components were determined by PIXE analysis. And the concentrations of 8 ions in the soluble component were analyzed by ion chromatography. In general, the mass size distribution of particulate matter was represented as a bimodal distribution. The mass size distributions of S$(SO_4^{2-}), K(K^+), Zn and NH_4^+$ skewed to the smaller size range and those of Si, Ca$(Ca^{2+}), Fe, Na^+ and Mg^{2+}$ skewed to the larger size range. They had roughly one peak in the fine and coarse particle region,respectively. On the other hand, the mass size distribution of Ti, Mn, Ni, Cu, $Cl^- and NO_3^-$ were represented as the bimodal distribution. Fe and Si in the aerosol particles extracted into pure water are existing in high insoluble state. Conversely, almost the whole of S is dissolved in water.

• BMB Reports
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• v.32 no.6
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• pp.605-609
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• 1999

Thioltransferase, also called glutaredoxin, is a general GSH-disulfide reductase of importance for redox regulation. Previously, the protein thioltransferase, now called S-type thioltransferase, was purified and characterized from Arabidopsis thaliana seed. In the present study, a second thioltransferase, called L-type thioltransferase, was purified to homogeneity from Arabidopsis thaliana leaves. The purification procedures included DEAE-cellulose ion-exchange chromatography, Sephadex G-50 gel filtration, and glutathione-agarose affinity chromatography. The purified enzyme was confirmed to show a unique band on SDS-PAGE and its molecular weight was estimated to be 26.6 kDa, which appeared to be atypical compared with those of most other thioltransferase. It could utilize 2-hydroxyethyl disulfide, S-sulfocysteine, and insulin as substrates, and also contained dehydroascorbate reductase activity. Its optimum pH was 8.5 and its activity was greatly activated by L-cysteine. When it was kept for 30 min, it appeared to be very stable up to $70^{\circ}C$. It was activated by $MgCl_2$ and, on the contrary, inhibited by $ZnCl_2$, $MnCl_2$, and $AlCl_3$.

• BMB Reports
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• v.28 no.2
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• pp.170-176
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• 1995

Rhodanese from mouse liver was purified to near homogeneity by ammonium sulfate precipitation, CM-Sephadex ion exchange, hydroxyapatite and Sephacryl S-200-HR gel filtration chromatographies with a purification of 776 folds. The molecular weight was determined by Sephadex G-150 gel filtration and found to be 34.8 KDa. SOS-PAGE showed molecular weight 34 KDa and two identical subunits splitting by aging for 3 weeks at $-70^{\circ}C$ the molecular weight of which was 17 KDa. The optimal pH of enzyme activity was 9.4 and the pI value of the enzyme was 6.6. Rhodanese showed the optimal reaction temperature of $25^{\circ}C$ and near linear increasing pattern until 10 min. incubation. $K_m$ values of rhodanese for KCN and $Na_{2}S_{2}O_{3}$ as substrates were 12.5 mM and 8.3 mM, respectively. Rhodanese activity was inhibited by more than 70% at a concentration of 100 ${\mu}M$ of $Ni^{2+}$, $Zn^{2+}$, $Cd^{2+}$, $Hg^{2+}$ and $Cu^{2+}$. Other metal ions, such as $Mn^{2+}$, $Mg^{2-}$, $Ca^{2+}$, and $Fe^{2+}$ showed no effect on rhodanese activity.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1994.04a
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• pp.257-257
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• 1994

생쥐의 장내세균으로 부터 황산전이 반응을 촉매하는 효소인 sulfotransferase를 생산하는 균주를 분리하였으며 동정결과 Haemophilus속 균주로 확인되어 Haemophilus K-12라 명명하였다. 균의 성장과 효소활성과의 관계를 보면 균은 10시간에서 완전히 성장하였으며 효소활성도 이와 비례하였다. Haemophilus K-12의 배지조성에 따른 sulfotransferase의 생산성을 Brain Heart Infusion 배지에서의 생산성과 비교해보면 탄소원으로는 sucrose가 0.2%농도에서 584%로 가장 좋았으며 질소원으로는 yeast extract가 266%로 가장 좋았다. 공여체로 PNS를 최종농도 1mM로 하여 배지에 첨가하였을때 212%로 가장 높은 효소증가를 보였다. 2가금속이온에 의한 효소증가는 현저하지 않았으며 $Mn^{2+}$이 107%로 가장 높았고$Zn^{2+}$와 EDTA에 의해서는 저해를 받았다. 이상의 결과를 종합하여 균배양을 위한 이상적인 배지조성을 sucrose 0.2%, yeast extract 1%, $Na_2$HPO$_4$ 0.25%, NaCl 0.5%로 결정하였다. 결정된 최적배지에 균을 10L 배양하여 초음파 처리, 원심분리한 것을 70 % ammonium sulfate fractionation, DEAE-cellulose column chromatography를 2회, Hydroxyapatite column chromatography, chromatdfocusing column chromatography, Silica PAE column chromatography, Sephacryl S-300 superfine column chromatography를 행한결과 specific activity가 6.76 umoie/min/mg protein인 효소액을 얻었으며 homogeneous enzyme였다. 이렇게 해서 얻은 효소를 이용하여 수용체 기질 특이성을 측정한 결과 1-naphthol이 phenol을 100%로 하였을 때 233%로 가장 좋았으며 Eubacterium A-44 sulfotransferase의 좋은 기질이었던 p-acetaminophenol, tyramine, 9-phenanthrol은 좋은 기질이 되지 못했다. 이상의 결과로 미루어 보아Haemophilus K-12 sulfotransferase는 지금까지 보고된 bacterial sulfotrasferase와는 다른 효소로 사려되며 효소반응기전의 규명이 이루어지면 산업적 응용이 가능할것으로 사료된다.