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Immunomodulatory Activities by Difference in Molecular Size of the Proteoglycan Extracted from Ganoderma lucidum IY009 (Ganoderma lucium IY009 유래 단백다당류의 분자량 차이에 따른 면역증강활성)

  • Lee, June-Woo;Baek, Seong-Jin;Bang, Kwang-Woong;Kim, Yong-Seuk;Kim, Kwang-Soo;Chun, Uck-Han
    • The Korean Journal of Mycology
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    • v.29 no.1
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    • pp.15-21
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    • 2001
  • This study was conducted to investigate the immunomodulatory activities of proteoglycan extracted from cultured mycelia of Ganoderma lucidum IY009. The proteoglycan contained two polymer peaks, one was the higher MW peak of 2,000 kD and the other was low peaks of 12kD. To understand the part of strong pharmaceutical activity between two peak, the proteoglycan was separated by ultrafiltration and column chromatography and then examined the various pharmaceutical effects. High molecular weight fraction possesing high content of ${\beta}-linked$ glucan was exhibited high antitumor activity, against sarcoma 180 bearing ICR mouse. And also, anticomplementary activity was highly observed in high molecule fraction than low it fraction. When the raw 264.7 and murine peritoneal macrophage treated with low fraction, high fraction and other stimuli. The activities inducing tumor necrosis factor of the high factions were $2.2{\sim}2.5$ times stronger than that of low fraction.

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Physicochemical characteristics of garlic (Allium sativum L.) shoots from different areas in Namhae (남해지역 마늘종의 이화학적 특성)

  • Yoon, Hwan-Sik;Kang, Min-Jung;Hwang, Cho-Rong;Sim, Hye-Jin;Kim, Gyeong-Min;Shin, Jung-Hye
    • Food Science and Preservation
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    • v.21 no.3
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    • pp.321-327
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    • 2014
  • This paper introduces the physicochemical characteristics of garlic shoots investigated in the 8 areas of Namhae, Gohyeon-myeon (GHM), Nam-myeon (NM), Samdong-myeon (SDM), Namhae-eup (NHE), Seo-myeon (SM), Seolcheon-myeon (SCM), Idong-myeon (IDM) and Changsoen-myeon (CSM). The moisture was $80.15{\pm}0.37{\sim}83.87{\pm}0.15g/100g$. The crude protein and lipid were $1.59{\pm}0.02{\sim}2.25{\pm}0.09g/100g$ and $0.35{\pm}0.01{\sim}0.48{\pm}0.03g/100g$, respectively. Ash was $0.65{\pm}0.03{\sim}0.71{\pm}0.03g/100g$. In addition, the total levels of phenol and flavonoid were searched as $33.02{\pm}1.78{\sim}58.41{\pm}1.08mg/100g$ and $11.60{\pm}3.36{\sim}23.95{\pm}2.04mg/100g$, respectively. The garlic shoots in IDM, where the highest total chlorophyll level detected as $170.58{\pm}1.04{\mu}g/g$ and in CSM, where the lowest total chlorophyll level investigated as $116.65{\pm}0.00{\mu}g/g$, were unveiled. The mineral elements (S, Ca, Fe, K, Mg, Na, Mn and Zn) were analyzed by ICP-OES, and K was the most highly contained mineral with $7257.0{\pm}87.9{\sim}10070.0{\pm}9.7mg/kg$. Significant feature of garlic shoots in IDM and NHE was that the total amounts of mineral were relatively higher than other regions as 21,143.5 mg/kg and 20,581.2 mg/kg, respectively. The cultivating regions were revealed as the one of important matters with the level of allicin ($78.03{\pm}6.15{\sim}203.43{\pm}18.47mg/kg$). In particular, the level of allicin of garlic raised in SDM ($203.43{\pm}18.47mg/kg$) was approximately 2.2~2.6 times higher than those in CSM ($92.86{\pm}8.70mg/kg$) and SCM ($78.03{\pm}6.15mg/kg$). The sulfur compounds content of diallyl trisulde was remarkably higher, especially the garlic shoots in IDM with $830.62{\pm}14.69{\sim}1281.10{\pm}27.71mg/kg$.

Studies on the Nutritional Value of Elderberry (Sam bucus canadensis) Fruits (Elderberry(Sam bucus canadensis) 과실(果實)의 영양가(營養價)에 대(對)하여)

  • Park, Kyo Soo
    • Journal of Korean Society of Forest Science
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    • v.67 no.1
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    • pp.42-49
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    • 1984
  • The elderberry was known to the ancients for its medicinal properties, and in Europe the inner back was formerly administered as a cathartic. The flowers contain a voletile oil, and serve for the distilation of elder-lower water, used in confectionery, perfumes and lotions. The leaves are employed to impart a green colour to fat and oil, and the leaves and bark emit a sickly odour, believed to be repugnant to insect. With its unique flavor and natural food colour, commercial processing companies used the fruit mainly in the making for jam, jelly, pies, juice, and wines. Its vitamin-C content is reported by Andross (1941) as 25-30mg/100g. Harvesting and processing have been mechanized to some extent. However, the cotains with nutritional value has not been reported yet. In the present study the various contains with nutritional value in the fresh elderberry juice is reported by the quantitative analysis. In this study results obtained can be summarized as follows. 1) The fresh elderberry juice contained following mineral elements; calcium 0.012%, magnesium 0.023%, potassium 0.10%, sodium 0.0019%, iron 0.0009%, cobalt 0.0002%, zinc 0.0004%, copper 0.0001%, phosphorus 0.036%, manganese 0.0006%, iodide $1{\mu}g/g$. 2) Five kinds of vitamines were also found ; vitamin-$B_1$ $0.1{\mu}g/g$, vitamin-$B_2$ $0.5{\mu}g/g$, vitamin-C 0.3mg/g, niacin $14{\mu}g/g$, choline chloride 0.3mg/g. 3) Fresh elderberry juice also contains crude protein 1.10%, fat 0.26%, carbohydrate 6.9%, pectin 0.76%, tannin 0.89%, ash 0.80%, water 90.9% and 34.3 cal/100g. 4) The absorption spectrum of the purplishblack color of fresh elderberry juice has a peak between 523-530mm.

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Studies of the components in Sedum sarmentosum Bunge as a materials of vegetable health (야채 건강음료 재료로서의 돌나물에 관한 성분 연구)

  • 김희아;홍철희;정형석
    • Culinary science and hospitality research
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    • v.8 no.1
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    • pp.55-69
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    • 2002
  • Sedum Sarmentosum Bunge has been used as medical material and food in Korea. To upgrade it's value as a materials of beverage, I research and analysed nutritive substance and taste. Inorganic components content was included in the order of Ca, mg, P, K, Na, in line, and there were little Fe, Cu, Zn. It appeared much Ascorbic acid contents in May sample for March sample, but it was included less than other Green Vegetables. Chlorophyll contents decreased about 37.8% and 37.6% to the first each contents after seven days resulting measuring May sample and March sample in room temperature. There was much difference in appearance. Sedum Sarmentosum Bunge included a little polyphenol contents. Polyphenol contents decreased a little in five days in May sample and March sample. I measured phenol compounds of garlic acid, vani11ic acid, caffeic acid, p-coumaric acid, ferulic acid, most of consisted in corrected forms. Fragrance components were confirmed 9 kinds in March sample, 11 kind in May sample the common components in May sample and March sample were 7-octen-4-o1.

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Component Analysis and Antioxidant Activity of Oenanthe javanica Extracts (돌미나리 추출물의 함유성분 분석과 항산화 활성)

  • Hwang, Seok-Jun;Park, Sung-Jin;Kim, Jong-Dai
    • Korean Journal of Food Science and Technology
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    • v.45 no.2
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    • pp.227-234
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    • 2013
  • The purpose of this study was to determine the possibility of using Oenanthe javanica as a natural health food source. To accomplish this, its general and biological activities were measured. Its carbohydrate, crude protein, crude lipid, and ash contents were 44.7, 9.8, 8.9, and 27.8%, respectively. The K content was largest for minerals followed by Ca, P, and Mg, which means that Oenanthe javanica is an alkali material. The concentrations of total phenol and flavonoids of OJE were $88.9{\pm}0.46$ mg GAE/g, and $28.6{\pm}0.64$ mg QE/g, respectively. Gallic acid, catechin, chlorogenic acid, and caffeic acid in OJE as measured by using HPLC were $0.9{\pm}0.23$, $1.2{\pm}0.19$, $227.1{\pm}0.62$, and $4.0{\pm}0.35$ mg/g. The DPPH and ABTS radical scavenging activities of OJE were 72.2%, and 66.1%, respectively, at $1,000{\mu}g/mL$. The FRAP and reducing power of OJE were 0.79, and 0.41 absorbance units value respectively, at $1,000{\mu}g/mL$. OJE possessed significant antioxidant properties, which suggests its great potential as a functional ingredient for food applications.

The Optimal Combination of Major Nutrients Computed by the Homès Systematic Variation Technique II. Determination of the Optimal Combination of Cations K:Ca:Mg in Fertilization on the Various Grassland Soils (Homes 방법(方法)에 의(依)한 다량요소(多量要素)의 적정시용(適正施用) 비율(比率) 결정(決定)에 관(關)한 연구(硏究) II. 초지토양별(草地土壤別) 음(陰)이온 N : Ca : Mg 적정시용(適正施用) 비율(比率))

  • Jung, Yeun-Kyu;Yoon, Sang-Gi;Weinberger, P.
    • Korean Journal of Soil Science and Fertilizer
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    • v.14 no.1
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    • pp.37-43
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    • 1981
  • This pot experiment was conducted to find out the optimal fertilization ratio of the cations(K: Ca: Mg) in various soil conditions. Soil samples were collected from uncultivated mountains and hills where development for grass lands is under consideration. The optimum ratios were computed by the Homes systematic variation technique. The results were summarized as follows : 1. The optimum fertilization ratios of the cations (K : Ca : Mg) for the high yields of grass-clover mixed sward at various areas were obtained (Table 4) 2. The relatively high optimum fertilization ratios of K in Daegu and Taegwalryong, Ca in Unbong, and Mg in Cheju district gave the high yields of clover in mixed sward. They were positively related to the low contents of the above cations in the soils or the relatively low content ratio comparing with other cations. 3. The yields of clover in mixed sward were seriously affected by the changes in both the fertilization ratio and total concentration of cations comparing to those of the grass yields. As a consequence, total yields, quality, and botanical composition of the mixed sward were also affected. 4. The antagonis among the cations K, Ca, and Mg was known from the point of mineral contents of grass and clover. 5. The equivalent ratio K/(Ca+Mg) or grass was greatly increased by the range of 2.4 and 3.3 in the K-group treatment, which was higher than the critical level 2.2 of grass tetany, otherwise that. of clover by the range of 0.9 and 1.2. Therfore, it may be concluded that if the botanical composition of grass/legume sward is optimum, the incidence of grass tetany can be eliminated.

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A Study on Components of Pollen Load (花紛荷(pollen load)의 성분조성에 관한 연구)

  • Kim, Jung-Hun;Lee, Kyu-Han;Kim, Eul-Sang;Park, Sung-Bae
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.21 no.5
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    • pp.566-572
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    • 1992
  • Proximate composition and the contents of free sugars, minerals, fatty acids, amino acids and in vitro digestibility of mixed pollen load were analyzed. The protein in pollen load per 100g was 25.2g, lipid 5.4g, non-fibrous carbohydrate 50.0g and fiber 10.4. Free sugars in pollen load, as dry basis g per 100g, was fructose 26.6g, glucose 13.1g, sucrose 0.2g, maltose 1.3g, melezitose 0.02g and F/G ratio was 2.0. The content of mineral in pollen load was in the order K > P > Mg > Ca > Fe > Na > Mn > Zn > Cu. Unsaturated fatty acid of pollen load was about 69.1% and polyunsalturated fatty acid being about 50.3%. The ratios of SFA : MUFA : PUFA and PUFA : SFA were 0.61 : 0.37 : 1.00 and 1.63 respectively. The total amino acid contained in pollen load 22,337.5mg/100g and total essential amino acid was 39.2% of the total amino acid. proline was the most abundant amino acid (2,824.7 mg/100g),followed by Glu, Leu, Asp, Val, Phe, Ile, Ala and Thr. E/T ratio of protein in pollen load was 2.03 ; protein score, 65.2(Lys) : A/E ratio(egg), 61.7(Lys) : amino acid score, 51.7% after 1hr and on non-fibrous carbohydrate was 80.6%. But that of protein was 67.3% after 1hr, 75.5% after 2hr.

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Geochemistry and Water Quality in the Tidal Flat of Saemangum Area, West Sea of Korea in Summer (하계 새만금 갯벌의 수질 분포 및 지화학적 특성에 관한 연구)

  • Park, Gyung Soo;Park, Soung Yun;Lee, Sam Geun;Lee, Yoon
    • Journal of Wetlands Research
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    • v.6 no.1
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    • pp.133-147
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    • 2004
  • Environmental quality(water and sediment) was analyzed in the tidal flat of Saemangum of Jeonbuk Province, the west coast of Korea, using the 101 sediment samples and 69 water samples collected in September 4~13, 2001. Major water quality parameters with the means of 69 surface water samples are as follows; $25.51{\pm}0.68^{\circ}C$ for water temperature, $29.88{\pm}5.01$ for salinity, $1.40{\pm}0.78mg/L$ for COD, $0.352{\pm}0.417mg/L$ for DIN, and $0.027{\pm}0.023mg/L$ for phosphate, respectively. Higher values were found at the subestuary of Dongjin and Mangyung River, and lower values at the Saemangum embayment and Gomso Bay. There was a significant negative correlation between salinity and the other water quality parameters(p<0.0001) such as COD, nutrients, SS and N/P. This correlation suggested that the major pollution sources be from terrestrial inputs through tributaries in this area. Principal component analysis clearly revealed a spatial variation of water quality; stations with higher values of nutrients and COD located subestuary of tributaries. 14 sediment quality parameters including 8 trace metals were measured using the 101 surface sediment samples. Average values for the parameters are as follows; Al $2.28{\pm}0.92%$, Cd $0.61{\pm}0.27ppm$, Cu $8.95{\pm}4.06ppm$, Fe $1.19{\pm}0.37%$, Mn $182.31{\pm}77.45ppm$, Ni $10.83{\pm}4.97ppm$, Pb $15.20{\pm}4.35ppm$, Zn $41.34{\pm}34.62ppm$, COD $2.68{\pm}1.85mg/g\;dry$, AVS $0.04{\pm}0.08mg/g\;dry$, IL $1.29{\pm}1.08%$, water content $24.11{\pm}4.49%$, TN $0.02{\pm}0.02%$, TC $0.22{\pm}0.30%$. Spatial variations of sediment quality were not clear as water quality. Some higher values were found at the subestuary of Gum River and lower values at the other area. There was a significant positive correlation between the heavy metal concentrations and organic materials within the sediment(p<0.05). Enrichment factors showed the ranges of 1~2 for most of the metals in the sediment except zinc(1~6), indicating no serious exogenous input of heavy metals in the study area. Also, the heavy metal concentrations in the sediment were within the ranges found at the natural marine environments.

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Some hair mineral contents of non-violent criminal and normal control (건강인(健康人)과 비폭력(非暴力) 범죄자(犯罪者)의 두발(頭髮)중 일부 금속원소(金屬元素) 함량(含量))

  • Hong, Sung-Cheul;Kim, Doo-Hie
    • Journal of Preventive Medicine and Public Health
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    • v.26 no.1 s.41
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    • pp.110-125
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    • 1993
  • This study was designed to determine whether non-violent criminal and normal control on the basis of concentration of levels of trace mineral and toxic metal by analysis of human scalp hair. The subjects were selected 87 nonviolent criminal from a prison population and 120 normal control from periodic health checks for study. Hair samples were taken from the napes and Minnesota Multiple Personality Inventory (MMPI) was performed also. Five trace mineral (Zn, Cu, Mg, Fe, Na) and two toxic metal (lead, cadmium) contents were determined by an atomic absorption spectrometer. The contents of zinc and magnesium in hair of non-violent criminal were significantly lower than the control group (p<0.01). In the case of lead and cadimum, mean value of criminal group was significantly higher than control group. Significantly higher T-score of MMPI was seen in non-violent criminal group fur psychopathic deviate (Pd), paranoia scale (Pa), and Mania scale (Ma) than control group, but T-score of depression scale (D) was significantly higher in the control group. In the non-violent criminal group, the content of copper inversely proportion to T-score of Hs, D, Hy, Pd, Mf, Pa, Pt, Sc, Si except Ma, also Zinc inversely proportion to T-score of Hy, Mf, Pa, Pt. These results suggest that difference of some hair mineral contents exist between criminal and normal control group. Thus further studies are necessary to determine whether violent and nonviolent criminal group attributed biochemical imbalance with carefully constructed and controlled studies.

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Relationship between Violent Criminal Behavior and Imbalance of Scalp Hair Minerals in Man (두발중 미량 중금속과 필수금속의 과다 또는 과소의 불균형과 폭력범죄 행동과의 관련성 연구)

  • Kim, Doo-Hie;Jang, Bon-Ki;Lee, Duk-Hee;Hong, Sung-Chul;Kim, Byung-Hie
    • Journal of Preventive Medicine and Public Health
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    • v.27 no.1 s.45
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    • pp.25-43
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    • 1994
  • To estimate the factors to the inclination of the criminal violence, the content of trace minerals and toxic metals in the scalp hair were measured during the period from May 1992 to October 1992. One hundred eleven violent and 89 nonviolent criminal inmates of Taegu Correctional Institute were selected. The inmates of violent criminals were imprisoned by murder, robber, rape, injury and violent acts. Those of nonviolent criminals were swindle, larceny, and adultery and had no history of institutional violence. The contents of two toxic metals (cadmium, lead) and five trace minerals (Cu, Fe, Zn, Mg, Na) were determined by an atomic absorption spectrophotometer (IL. 551). The contents of cadmium and lead in hair of violent criminals were significantly higher as $0.56{\pm}0.14ppm,\;11.53{\pm}3.32ppm$, respectively, than $0.42{\pm}0.20ppm,\;9.63{\pm}4.31ppm$ of nonviolent group (p<0.01). But the level of copper was significantly lower than nonviolent group (p<0.05). The factors that had a significant correlation with the inclination of violence in multiple logistic regression analysis were cadmium (odds ratio=98.09), unmarried (odds ratio=0.39), many times of criminal history(odds ratio=1.57) and residence of rural area (odds ratio=0.44). The results suggest that the sub-toxic contents of cadmium and lead in the hair may be of potential effect on behavior, and the mineral analysis may be an important adjunctive diagnostic procedure. Further studies into this problem are necessary.

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