• Journal of Oral Medicine and Pain
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• 제23권4호
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• pp.343-352
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• 1998

This experiment was performed to investigate effects of zinc containing solution on the major normal flora Staphylococccus aureus, Streptococus mutans and Candida albicans and to observe the variation according to anionic change and concentration difference. Zinc chloride, zinc iodide and zinc acetate solution were added to werially diluted broth culture so that each final concentration might be 0.25%, 0.5%. 1%. After that, 100ul of each aliquot was spreaded on each selective media plate( Mannitol Salts Agar plate for Staphylococcus aureus, Mitis Salivarius Agar plate for Streptococcus mutans and Sabouraud Destrose Agar plate for Candida albicans). The % killing was calculated bu CFU count after incubation under the appropriate condition. 1. zinc iodide, zinc chloride, and zinc acetate solutions showed inhibitory effects on Staphylococcus aureus, Streptococcus mutans and Candida albicans. 2. The inhibitory effects on Staphylococcus aureus were ranked in order of ainc iodide, zinc chloride and zinc actate. 3. The inhibitory effects on Streptococcus mutans were ranked in orfer of zinc iodide, zinc chloride and zinc acetate. 4. the inhibitory effects on Candida albicans showed no difference among zinc iodide, zinc chloride and zinc acetate. 5. The inhibitory effects of zinc chloride and zinc acetate on Staphylococcus aureus and Streptococcus mutnas showed increasing pattern as the concentration increase. But the inhibitory effects of zinc iodide on Staphylococcus aureus and Streptococcus mutans showed no apparent difference according to concentrations and it was the case with the inhibitory effects of zinc iodide, zinc chloride and zinc acetate on Candida albicans.

• Asian-Australasian Journal of Animal Sciences
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• 제3권1호
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• pp.53-59
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• 1990

A study was conducted to investigate the release pattern of zinc form the zinc containing boluses and to see whether the released zinc can cure a zinc deficiency in sheep. Three sheep were used in this experiment and were fed a low zinc semi-synthetic diet throughout the experimental period. Each sheep was given a single pre-weighed zinc containing bolus when blood variables showed continuous zinc deficiency. The zinc containing boluses when placed within the reticulo-rumen of zinc deficient sheep, release zinc at the rate of 106.6 mg zinc/day for 39 days. At the end of depletion period there was a reduced feed consumption, plasma zinc concentration, plasma alkaline phosphatase activity and increased plasma zinc binding capacity which were 409 g, 0.18 mg/l, 87 U/l and 88.7% respectively and 521 g, 0.18 mg/l, 142 U/l, and 89.5% respectively before first and second blousing. After the administration of the first and second boluses, the feed consumption, plasma zinc levels and plasma alkaline phosphatase activities rose rapidly and far exceeded the starting values. The zinc binding capacity was reduced to 21.9% due to the administration of the first and second boluses. It is concluded that zinc boluses can be used for curing a zinc deficiency in sheep.

• 한국식품영양학회지
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• 제15권1호
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• pp.7-11
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• 2002

학령전 아동의 zinc absorption, excretion, balance에 대하여 연구한 결과는 다음과 같다. Zinc absorption은 남자아동의 경우 29.7%, 여자아동의 경우 23.3%, 평균 25.8%로 나타났다. 대상자기 zinc absorption은 zinc intake와는 상관성이 나타나지 않았으며, zinc balance와 유의적 상관(p<0.01)을 보였다. Zinc balance는 남자아동의 경우 평균 3.25mg/day , 여자아동의 경우 1.78mg/day , 평균 2.34mg/day 로 1명의 대상자를 제외하고 모두 positive balance를 보였다. Fecal zinc loss는 평균 6.31mg/day, urinary ziilc loss는 평균 0.16mg/day 였다. zinc intake는 focal zinc loss및 zinc balance에 유의적 영향을 미치는 것으로 나타났으며(p<0.01), urinary zinc는 intake의 영향을 받지 않았다. 우리나라 학령전 아동의 zinc absorption 및 balance 는 양호한 것으로 나타났다. 그러나 이 방면의 연구 자료가 많지 않아, 성장기 아동을 대상으로 한 zinc nutrition에 대한 구체적이고 지속적인 연구가 필요하다.

• 한국환경과학회지
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• 제5권3호
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• pp.317-327
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• 1996

This study was performed to develop the biological treatment technology of wastewater polluted with heavy metals. Zinc-tolerant microorganism, such as Pseudomonas chlororaphis which possessed the ability to accumulate zinc, was isolated from industrial wastewaters polluted with various heavy metals. The characteristics of zinc accumulation in the cells, recovery of the zinc from the cells accumulating zinc, were investigated. Removal rate of zinc from the solution containing 100 mall of Zinc by zinc-tolerant microorganism was more than 90% at 48 hours after inoiulation of the microorganisms. A large number of the electron-dense granules were found mainly on thIn cell wall and membrane fractions, when determined by transmission electron microscope. Energy dispersive X- ray spectroscopy revealed that the electron-dense granules were zinc complex with the substances binding Heavy metals. The zinc accumulated into cells was not desorbed by distilled water, but more than 80% of the zinc accumulated was desorbed by 0.1M-EDTA. The residues of the cells after combustion at 55$0^{\circ}C$ amounted to about 21% of the dry weight of the cells. EDS analysis showed that the residues were comparatively pure zinc compounds containing more than 79% of zinc.

• 한국식품영양과학회지
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• 제24권2호
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• pp.346-353
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• 1995

The dietary requirement for zinc to maintain optimally the various metabolic and physiological funcitons is still under study. Human beings adapt to reductions in zinc intake by reducing the rate of growth or zinc excretion. Reductions in dietary zinc beyond the capacity to maintain homeostasis lead to utilization of zinc from an exchangeable pool. Loss of a small, critical amount of zinc from this pool leads to both biochemical and clinical signs of zinc deficiency. Zinc requirements have been assessed by balance studies and factorial method. As tissue zinc status influences endogenous losses and the dietary needs, individuls in good status may require higher amounts of zinc than those in poor status. While plasma zinc is insensitive to reducitons in dietary zinc, it is regarded as a valid, useful indicator of the exchangeable pool of zinc. Plasma metallothionein concentrations may prove useful for identifying poor zinc status. It has been suggested that functional end point measurement is the new direciton for zinc requirement. However, determination of the functional response to a marginal zinc intake is difficult because of the lack of a specific, sensitive indicator of zinc status. Presently, no good method for assessment of human zinc requirements exists.

• Journal of Nutrition and Health
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• 제36권9호
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• pp.950-959
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• 2003

To assess zinc status by dietary intake and urinary excretion of preschool children in Busan and to evaluate the relationship of intakes of food and nutrient with urinary zinc excretion, zinc food frequencies of 40 common foods affecting intakes of zinc by food fequency method, nutrient intake by 24hr recall and 24hr urinary zinc excretion were measured with 97 preschool children. The mean zinc intake was 4.29 mg and 43.0% of RDA. The mean zinc intake per 1,000 kcal was 3.09 mg.97.9％ of subjects had zinc intake less than 75％ of RDA. Grains food group was the primary source of zinc intake and supplied 38.9% of the total daily zinc intake. Altogether, plant food products supplied 49.7% of zinc intake. The mean urinary zinc excretion and zinc excretion per gram of creatinine were 0.19 mg and 1.00 mg respectively. The urinary zinc excretion showed positive significant correlations with height and weight (p < 0.05, p < 0.05) , urine volume and urinary creatinine excretion (p < 0.05, p < 0.001) , urinary zinc excretion per creatinine (p < 0.001) , urinary zinc excretion per weight (p < 0.001) , intakes of energy and carbohydrate (p < 0.05, p < 0.01) and usual intake of zinc from eggs food group (p < 0.05) . In conclusion, these results show that the zinc intake of preschool children is low and that sources of dietary zinc are mainly plant foods, suggesting low bioavailability. So nutritional education is needed in order to inc-rease usual intake of animal food group. Interpretation of urinary zinc excretion data is complicated by current uncertainty about "normal" zinc level at this age group. Further studies are needed to obtain extensive data on urinary zinc excretion for this age group.age group.

• Journal of Nutrition and Health
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• 제26권9호
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• pp.1110-1117
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• 1993

The purpose of this was to analyse zinc intakes and effect of Zn(30mgZnSO4/day) supplementation on plasma zinc level, serum HDL-cholesterol and serum Alkaline Phosphatase (AP) activity of Korean adults. The men consumed 8.52($\pm$2.08)mg of zinc, and the women consumed 6.4($\pm$2.62)mg of zinc. Although protein intakes of subjects were lower than normal values. The first source of zinc was cereal and grain group, the second was meat, fish, egg and soybean group. Two food groups supplied about 80% of zinc. After two weeks of zinc supplementation, the zinc concentration in plasma was significantly increased. The highest plasma zinc level was 78.80ug/dl(men), 76.04ug/dl(women) at 2 weeks after zinc supplementation(p<0.05). Serum DHL-cholesterol was significantly decreased by zinc supplementation. The lowest serum HDL-cholesterol level was 39.29mg/dl(men), 44.84mg/dl(women) at 4 weeks after zinc supplementation(p<0.01). Serum AP activity was significantly increased by zinc supplementation. The highest AP activity was 86.40units/L(man), 67.93units/L(women) at 2 weeks after zinc supplementation(p<0.05). It seems that the supplementation of 30mg ZnSO4/day can be beneficial for improving zinc nutriture. However it can be negative factor on coronary heart disease because serum HDL-cholesterol was significantly decreased(p<0.01)

• Preventive Nutrition and Food Science
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• 제2권1호
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• pp.35-41
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• 1997

The pancreas is an important organ in the maintenance of zinc homeostasis. Endogenous zinc is con-tinuously secreted via pancreatic exocrine fluid or to a lesser extent in bile. Much of the endogenous secretion must be reabsorbed to sustain zinc homeostasis. The objective of this study was to estimate the relative size of the pancreatic/biliary zinc pool in comparision to the dietary zinc intake, and to study the effect of the phytate and calcium on the zinc homeostasis using a rat model. At the termination of the experiment, pan-creatic/biliary fluid was collected from the rats. Both radioactivity and total zinc were measured and the relative size of the pancreatic/biliary zinc pool was estimated. To determine the effect of phytate and calcium on zinc homeostsis, dietary zinc intake, the amount of zinc in pancreatic.biliary fluid and fecal zinc excretion were measured. The flow rate of pancreatic/biliary fluid, as corrected for tubing constriction, gives the corrected zinc concentration in the pancreatic/biliary fluid was 2.2 times higher than dietary zinc intake. To maintain zinc homeostasis, zinc absorption/reabsorption was very efficient in the current model; 76%, 88% of absorption/reabsorption for low calcium group and high calcium group 81% for phytate group and non-phytate group, respectively.

• 대한지역사회영양학회지
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• 제3권3호
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• pp.389-396
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• 1998

This study was intended to examine the zinc status and effect of zinc supplementation on the zinc nutritional status of the elderly living in the Ulsan area. The zinc intake of 207 subjects(male 97, female 110) was measured by a 24-hour dietary recall and food frequency method. Biochemical analysis were conducted from blood and urine samples to evaluate the changes of zinc nutriture with zinc supplementation. The average dietary zinc intake of subjects was $7.7\pm{2.8mg}$ for male and $7.5\pm{2.6mg}$ for female, which were 51.3% and 62.3% of Korean RDA respectively. The first source of zinc was cereal and grain(36%), and the second was eggs and milk group(27%). After 8 weeks of zinc supplementation, the serum zinc content was significantly increased(p<0.01), although the serum copper content was not significantly decrease. Serum HDL- cholesterol level was not significantly decreased with zinc supplementation. Serum alkaline phosphatase(ALP) activity and urinary zinc excretion were significantly increased(p<0.05). The urinary Zn/Cr was not significantly increased. It is suggested from the results that the daily zinc supplementation can be effective to improve zinc nutriture.

• 대한지역사회영양학회지
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• 제2권1호
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• pp.52-62
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• 1997

This study was intended to examine the zinc status of free-living adult women living in the Taegu region. Zine intake of 102 female subjects was measured by food frequency method for 3 consecutive days. Urinary zine was assessed from urine samples collected fir twenty four hours. Average dietary zinc intake of adult female subjects using food record was 5.9$\pm$1.8mg which was 49$\%$of Korean RDA. When zine intake by frequency method was higher than by food record. Average urinary zine intake by frequency method was higher than that by food record. Thus, it appeared that zinc intake by frequency method was higher than that by food record. Average urinary zinc excretion of 102 adult female subjects was 0.28$\pm$0.16mg, which belonged to marginal zinc deficiency range. Fifty nine of 102 subjects showed marginal zinc deficiency as assessed by urinary zinc excretion. If we compare the zinc status of adult female subjects by age group, zinc intake in the 50s was significantly higher than any other age groups. However, there were no significant differences in zinc status according to BMI groups and BMI groups. Significant correlations were found between zinc intake and energy, protein, carbohydrate and fat intake. Based on dietary zinc intake and urinary zinc, we concluded that zinc status of adult female living in the Taegu region is marginally deficient.