• Journal of Microbiology and Biotechnology
• /
• v.20 no.1
• /
• pp.193-201
• /
• 2010

A specific and rapid real-time PCR assay for detecting Ralstonia solanacearum in horticultural soil and plant tissues was developed in this study. The specific primers RSF/RSR were designed based on the upstream region of the UDP-3-O-acyl-GlcNAc deacetylase gene from R. solanacearum, and a PCR product of 159 bp was amplified specifically from 28 strains of R. solanacearum, which represent all genetically diverse AluI types and all 6 biovars, but not from any other nontarget species. The detection limit of $10^2\;CFU/g$ tomato stem and horticultural soil was achieved in this real-time PCR assay. The high sensitivity and specificity observed with field samples as well as with artificially infected samples suggested that this method might be a useful tool for detection and quantification of R. solanacearum in precise forecast and diagnosis.

• Journal of Navigation and Port Research
• /
• v.34 no.7
• /
• pp.567-575
• /
• 2010

Ningbo Port develops quickly in recent years and with the combination of Ningbo Port and Zhoushan Port, it becomes another important port in the Shanghai International Shipping center. Competition between the two biggest ports in this area can't be avoided. The goal of this paper is investigating the competence of port from two angles, which are spatial structure of the ports cluster and neighboring ports' attraction to cargoes at conjunct hinterlands. The paper firstly uses the HHI index model and shift-share method to qualitatively and quantitatively analyze the data of container throughputs of ports in the shipping center, in order that investigates the spatial structure of ports cluster. Basing on above researches, the paper employs the location quotient to study Shanghai port and Ningbo port's hinterland distributions at Zhejiang province. The conclusion of this study:(I)the ports cluster of the Shanghai International Shipping Center is highly centralized, and undergoes a porcess of first centralization then decentralization since the mid of 90s, last century. (2)Hinterlands of Shanghai port includes: Hangzhou, Jiaxing, Huzhou and Quzhou districts; Hinterlands of Ningbo port includes: Ningbo, Taizhou and Zhoushan districts.

• Journal of the Korean Institute of Rural Architecture
• /
• v.15 no.1
• /
• pp.55-62
• /
• 2013

This study is to solve this problem, as the center of China in the east started the investigation. In eastern China : rapid economic growth, Population is concentrated, Migration of rural population to large cities. So I chose this as the survey area. Including central and eastern China : Shandong Province, Jiangsu Province, Zhejiang Province, Fujian Province, Anhui Province, Jiangxi Province and Shanghai. Research studies as a whole is divided into three main parts: The first is the basic situation of rural housing survey. Including : housing age, wall materials, roof form, house equipment, housing composition, etc. Then home for further investigation of these houses exist in the main room on the device and the joint space problems. Finally, to address these issues, the survey specifically classified as a pure compound the family home and two cases. Through interviews, to understand the survey requirements for housing improvement. Finally, comparison summary, conclusion.

• Journal of Microbiology and Biotechnology
• /
• v.27 no.7
• /
• pp.1276-1280
• /
• 2017

The mcr-1 gene is a new "superbug" gene discoverd in China in 2016 that makes bacteria highly resistant to the last-resort class of antibiotics. The mcr-1 gene raised serious concern about its possible global dissemination and spread. Here, we report a potential anti-resistant strategy using the CRISPR/Cas9-mediated approach that can efficiently induce mcr-1 gene knockout in Escherichia coli. Our findings suggested that using the CRISPR/Cas9 system to knock out the resistance gene mcr-1 might be a potential anti-resistant strategy. Bovine myeloid antimicrobial peptide-27 could help deliver plasmid pCas::mcr targeting specific DNA sequences of the mcr-1 gene into microbial populations.

• Bulletin of the Korean Mathematical Society
• /
• v.50 no.2
• /
• pp.485-498
• /
• 2013

In this paper, the Schur convexity is generalized to Schur $f$-convexity, which contains the Schur geometrical convexity, harmonic convexity and so on. When $f$ : ${\mathbb{R}}_+{\rightarrow}{\mathbb{R}}$ is defined as $f(x)=(x^m-1)/m$ if $m{\neq}0$ and $f(x)$ = ln $x$ if $m=0$, the necessary and sufficient conditions for $f$-convexity (is called Schur $m$-power convexity) of Gini means are given, which generalize and unify certain known results.

• Bulletin of the Korean Mathematical Society
• /
• v.49 no.1
• /
• pp.33-47
• /
• 2012

In this paper, the log-convexity of another class one-parameter mean is investigated. As applications, some new upper and lower bounds of logarithmic mean, new estimations for identric mean and new inequalities for power-exponential mean and exponential-geometric mean are first given.

• Journal of Fashion Business
• /
• v.11 no.4
• /
• pp.152-172
• /
• 2007

This research suggest the correspondent sizes of women's and men's ready-made clothes to export to China on the basis of KS size specification. The results of this study, with the cooperation of Sejung and Searte Companies exporting clothes to Ningbo City, Zhejiang Province in China, are as follows: 1. Corresponding Dimensions of China Women's Clothes According to Morrison's relative deviation method, the body measurement characteristics of Chines and Korean women in their 30s and 40s were compared. As a result, Chinese women were bigger in height, arm length, abdomen girth, and upper arm girth but smaller in shoulder length and shoulder width. The cluster analysis for body type classification was as follows: Type 1, tall and well-developed, was 34.8%. Type 2 with weaker upper body, medium height, narrow shoulders and a slim waist was 45%. Type 3 with stronger upper body, shortest height and wide shoulders was 20.2%. 2. Corresponding Dimensions of China Men's Clothes Based on the drop standard of KS K 0050, the body types of Chinese men in their 20s were classified. A body types were 55.77%, Y body types were 32.16%, B body types were 11.55% and BB body types were 1.51%. According to the criteria of ready-made dimensions of normal body type suggested in Korea Standards, and in consideration of the basic distribution reality of body dimensions, representative 5 sizes were selected. 3. The corresponding size specification of China women's and men's clothes based on those of KS size specification were presented here, showing product sizes and physical items in detail.

• Journal of Fashion Business
• /
• v.11 no.1
• /
• pp.61-74
• /
• 2007

Photographic measurement was first made with the subjects of 190 males in their 20s residing in the Ningbo area, Zhejiang Province in China. In this second report, lower body shapes were classified and discriminated by using indirect measurement, measurement items, and lower body analysis. The following sums up the research: 1. The subjects were $8.85^{\circ}$ (hip breadth angle), $1.58^{\circ}$ (abdomen upper angle), $11.80^{\circ}$ (hip upper angle), and $5.12^{\circ}$ (lateral lower body posture angle). 2. The subjects of Chinese males in their 20s showed three types of lower bodies: Bow Legs & Slight Slant of Lateral Lower Body Type (30.5%)-gap between legs, curve waist-hip contour, average abdomen-hip profile, and lateral lower body posture were slightly slanted forward. Adjacent Straight Legs & Slight Slant of Lateral Lower Body Type (35.8%)-adjacent straight between legs, curve waist-hip contour, slim abdomen-hip profile, and lateral lower body posture were slightly slanted forward. Balance Legs & Large Slant of Lateral Lower Body Type (33.7%)-average between legs, straight waist-hip contour, protruding hip profile, and lateral lower body posture were largely slanted forward. 3. Eight useful variables for the categorization of the subjects' lower body types were chosen through stepwise discriminant analysis, and the hit ratio of discrimination was 97.9%.

• Parasites, Hosts and Diseases
• /
• v.46 no.1
• /
• pp.45-48
• /
• 2008

To evaluate the usefulness of the Korean Isolate-1 (KI-1) antigen for serodiagnosis of toxoplasmosis, antigen profiles of KI-1 tachyzoites were analyzed in comparison with RH tachyzoites by SOS-PAGE and immunoblotting. ELISA was performed on latex agglutination (LA)-positive and negative serum samples using KI-1 and RH antigens. Immunoblotting of the KI-1 antigen showed multiple antigen bands with molecular sizes of 22-105 kDa. Among them, 1 and 6 common bands were noted against a KI-1-infected and a RH-infected human serum, respectively, which represented differences in antigenic profiles between KI-1 and RH tachyzoites. However, all 9 LA-positive human sera were found positive by ELISA, and all 12 LA-negative sera were negative by ELISA; the correlation between the ELISA titers and LA titers was high (r = 0.749). Our results suggest that tachyzoites of KI-1 may be useful for serodiagnosis of human toxoplasmosis.

• Asian Pacific Journal of Cancer Prevention
• /
• v.15 no.14
• /
• pp.5819-5823
• /
• 2014

Purpose: To investigate the expression of hypoxia-inducible factor prolyl hydroxylase 3 (HIFPH3) in non-small cell lung cancer (NSCLC) and explore the correlation of HIFPH3 expression with lymph node metastasis and microvessel density (MVD). Materials and Methods: A total of 73 cases of NSCLC specimens, 24 cases of para-cancerous tissues, and 20 normal pulmonary tissues were collected for HIFPH3 and CD31 immunohistochmical (IHC) study. Microvessel density (MVD) of the NSCLC tissues was also determined based on the expression of CD31. Results: The expression of HIFPH3 in carcinoma tissue was statistically higher than para-cancerous and normal pulmonary tissues (${\chi}^2=48.806$, p<0.05). Compared withthe negative lymph node metastasis group, the lymph node metastasis group showed significantly higher HIFPH3 expression (${\chi}^2=6.300$, p<0.05). The strong HIFPH3+group displayed a significantly higher MVD than weak HIFPH3+ and HIFPH3- groups (p<0.05). No differences in positive HIFPH3 expression were noted regarding the tumor diameter, age, smoking status, gender of NSCLC patients, tumor size, histopathology, or differentiation. Conclusions: HIFPH3 expression in human NSCLC lesions is significantly higher than that in para-cancerous and normal lung tissues and is positively associated with lymph node metastasis and MVD.