• Korean journal of applied entomology
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• v.62 no.3
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• pp.131-137
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• 2023

The striped fruit fly, Zeugodacus scutellata (Diptera: Tephritidae), is one of important pests in Cucurbitaceae plants. The cuelure is currently using with various forms for surveillance to the Z. scutellata male as well as Z. cucurbitae, Z. tau, and Bactrocera tryoni, which are categorized as prohibited-quarantine fruit flies in Korea. This study was conducted to verify the attraction effect of applying the cuelure in different forms in field conditions. In this study, we used cuelure by 3 different forms as solid, liquid, and wax to striped fruit flies using the Steiner trap. The trap was placed in two Jeju and one Gyeongbuk regions and the number of attracted flies by each cuelure form is monitored by every 2 weeks during 2021. Attraction effect on Z. scutellata was significant difference by different cuelure forms in two Jeju regions. Liquid and wax forms of cuelure showed high attraction number to the Z. scutellata male compared with solid form when flies were occurred with high population from July to August in Jeju. However, there was no significant difference on attraction effect at low population in Juju and Gyeongbuk by different forms. Based on field assay, liquid and wax form of cuelure are recommended for surveillance against Z. scutellata male. Furthermore, these results also strongly suggest that the attractant, liquid and wax forms of cuelure, could be effective for surveillance to quarantine fruit flies such as Z. cucurbitae, Z. tau, and B. tryoni in Korea.

• Korean journal of applied entomology
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• v.59 no.1
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• pp.25-35
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• 2020

The striped fruit fly, Zeugodacus scutellata, is endemic in Korea, but it has been regarded as one of the serious quarantine pests throughout the world. Sterile insect release technique (SIT) has been used to eradicate quarantine fruit flies. This study developed a technique to generate sterile males and applied SIT to control Z. scutellata. First of all, the reproductive systems of Z. scutellata adults were examined with fluorescent microscope. Polytrophic ovaries comprises of around 100 follicles with developing oocytes. Each follicle contains an oocyte with several nurse cells and are surrounded with follicular epithelium. Oocyte development began at 10 days after adult emergence (DAE) and formed chorionated oocytes after 20 DAE. On the other hand, male testes were well developed just after adult emergence. The vas deferens was filled with motile sperms. To generate sterile males, different doses (0~1,000 Gy) doses of electron beam were irradiated to 3~5 days old pupae of Z. scutellata. When male pupae were irradiated with electron beam at 200 Gy, they developed and mated with females without any significant difference compared to untreated males. Although the untreated females mated with the 200 Gy-irradiated males laid eggs, no eggs did not hatch. The 200 Gy-irradiated males were then applied to untreated male and female flies in a density ratio of 1:9 (untreated males : treated males). The laid eggs suffered significant infertility. These results suggest that electron beam-irradiated pupae at 200 Gy resulted in male sterility and the resulting males would be applied to SIT.

• Korean journal of applied entomology
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• v.59 no.1
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• pp.15-22
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• 2020

Two tephritid fruit flies are infesting pumpkins in Korea. Both are classified into genus of Zeugodacus. The striped fruit fly, Z. scutellata, males are attracted to a lure called Cuelure (CL), which has been used to monitor the occurrence of this fruit fly in the crop field. In contrast, no effective male lure was not developed to monitor the pumpkin fruit fly, Z. depressa. Protein diet lure has been used to attract females of most fruit flies. The addition of terpinyl acetate (TA) was effective to increase the attractiveness of Z. depressa. This study aimed to monitor the occurrence of Z. depressa in pumpkin field with TA-protein diet lure. To validate the efficiency of TA-protein diet lure, Z. scutellata was monitored in a year of 2019 using CL and TA-protein diet lures, and the yearly monitoring data were compared. The occurrence patterns derived from both lures were similar except late season after October. The extended catches of TA-protein diet lure might be explained by the adult diapause induction of Z. scutellata at late September. Monitoring Z. depressa with TA-protein diet lure gave two peaks at mid July and August-September, in which more than 80% catches were females. Based on the attractiveness of TA-protein diet lure, its wettable powder with an addition of spinosad insecticide was formulated and sprayed to pumpkin crops. After 7 days post-spray, the control efficacy recorded more than 70%. However, the control efficacies decreased as the time progressed after the spray. These results demonstrated the application of TA-protein diet lure for monitoring occurrence of Z. depressa in pumpkin-cultivating field conditions. The wettable powder containing spinosad can be applied to develop a new control agent against two pumpkin fruit flies.