• Land and Housing Review
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• v.11 no.3
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• pp.69-74
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• 2020

Since the design Standard for Energy Conservation in Building was implemented in 2008 for the first time, building elements such as window and door should satisfy the minimum criteria to apply for a building. Though its regulation does not cover the whole building yet, recent demand to reduce energy consumption in building sector grows rapidly year by year and also draws a lot of interest to ensure the whole building level. For example, a Zero energy building, one of low-energy buildings, requires a customized solution to resolve the air leakage issue to meet the standards in achieving the high level of air tightness. In this study, six non-residential buildings were tested by fan pressurization method to observe the air tightness of whole building to suggest the construction guideline for air tightness of low-energy building. Five out of six tested buildings showed 0.27 to 1.16 h^-1 of number of air changes except one community center. These buildings were carefully constructed not only for building planning but also for parts where there was a concern of air leakage, thereby securing high levels of air-tightness. The construction skills were developed as a checklist to manage and supervise the construction site. It is our suggestion to use this checklist at construction sites for ZEB with the high level of air-tightness.

• Journal of Energy Engineering
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• v.27 no.3
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• pp.21-27
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• 2018

In accordance with the implementation of the Zero Energy Building Certification System, it for the activation and expansion of the private sector is being steadily upgraded. Also The government has set up a step-by-step mandatory roadmap until it is expanded to the private sector, starting with the public sector. We analyzed the energy requirements of existing buildings from 2016 to 2017 and the by load relationships of major factor. Of the existing buildings, 714 buildings in central and southern areas excluding residential buildings such as apartments and officetels were classified and their primary energy requirements were analyzed. As new design technologies are applied, the demand for energy from the passive side is steadily declining. In addition, there is a need to interpret various methods to improve the zero energy building certification standard in the point that the zero energy building pilot project is continuously carried out in relation to the activation of renewable energy supply.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.6
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• pp.2473-2481
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• 2015

Colon cancer is one of the leading causes of cancer-associated death worldwide. The prognosis for advanced colorectal cancers remains dismal, mainly due to the propensity for metastatic progression. Accordingly, there is a need for effective anti-metastasis therapeutic agents. Since a great body of research has indicated anticancer effects for curcumin, we investigated the effects of dendrosomal curcumin (DNC) on cellular migration and adhesion of human SW480 cells and possible molecular mechanisms involved. Different methods were applied in this study including MTT, Scratch and adhesion assays as well as real-time PCR and transwell chamber assays. Based on the results obtained, DNC inhibits metastasis by decreasing Hef 1, Zeb 1 and Claudin 1 mRNA levels and can reduce SW480 cell proliferation with $IC_{50}$values of 15.9, 11.6 and $7.64{\mu}M$ at 24, 48 and 72h post-treatment. Thus it might be considered as a safe formulation for therapeutic purpose in colorectal cancer cases.

• Toxicological Research
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• v.31 no.2
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• pp.151-156
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• 2015

5-Fluorouracil (5-FU) is commonly used for the therapy of colon cancer; however, acquired resistance to 5-FU is a critical barrier to successful treatment and the primary cause of chemotherapy failure. Epithelial-mesenchymal transition (EMT) is a process whereby cells undergo alterations in morphology and molecular characteristics promoting tumor progression and metastasis. Accumulating evidence shows that transition from epithelial to mesenchymal phenotype in cancer cells is associated with their resistance to chemotherapy. However, it is still poorly understood whether EMT is involved in acquired resistance to 5-FU. In this study, we developed an in vitro cell model, 5-FU-resistant HT-29 colon cancer cells, and characterized the differences in cellular morphology and molecular alterations between parental and resistant cells. In accord with mesenchymal-like morphology of 5-FU-resistant HT-29 cells, the expression of the mesenchymal marker fibronectin was significantly increased in these cells in comparision with that in the parental cells. Of interest, we also found a marked increase in the expression of EMT-inducing transcription factors Twist, Zeb1, and Zeb2. Finally, 5-FU-resistant cells showed enhanced migration in comparison with parental HT-29. Taken together, these results indicate that EMT could be associated with 5-FU resistance acquired by HT-29 cells. A specific role of each transcription factor found in this study will require further investigation.

• Journal of the Korean Society for Geothermal and Hydrothermal Energy
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• v.15 no.4
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• pp.16-23
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• 2019

Renewable energy systems are essential for the realization of zero energy building (ZEB). Moreover, the integrated system using solar and geothermal energy has been developed for heating, cooling and power of the building. However, there are few studies considering various design factors for system design. In this study, in order to develop the optimal design method for the system, the performance of the system was quantitatively compared and analyzed through dynamic simulation. Moreover, economic analysis was conducted based on the results of system performance. Through the performance and economic analysis results, the optimal design method of the tri-generation system was proposed.

• Journal of Energy Engineering
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• v.26 no.4
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• pp.92-99
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• 2017

In Korea, the zero energy building was designated as the 7 new industries in the Ministry of Land and the 8 new industries in the Ministry of Industry. In order to maximize the insulation performance of the building envelope, improve the efficiency of building equipment, We are aiming. It is necessary to analyze the energy requirements of the buildings (cooling, heating, hot water supply, lighting, ventilation) of buildings with energy efficiency level of 1++ which is equivalent to the zero energy building certification system in Korea, It is aimed to be used as basic data for the advancement of energy building certification system. Zero Energy Building certification is estimated to be 61 buildings by 2017, and the approximate reference value and the first energy requirement for each of the five loads are calculated considering passive and active aspects. It is difficult to say that it is a clear standard because there is a small sample of data for calculating the load standard. However, it is necessary to interpret various methods in order to upgrade the Zero Energy Building certification standard in the future.

• Journal of Energy Engineering
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• v.29 no.2
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• pp.61-67
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• 2020

ESS-based buildings are being widely studied as an effective methods for saving energy with ZEB, BEMS, and FEMS. However, in large scale buildings, there are many energy-consuming facilities, so it is necessary to identify important energy-consuming facilities to build a real-time measurement system. In addition, there are a myriad of factors that affect the dependent variable of energy use, therefore there is a limitation that effective energy management is difficult. Therefore, this study applied the measurement boundary setting methodology according to the energy supply status through due diligence for the demonstration ESS distribution logistics facility, and suggested the methodolgy for presenting priority for the construction of the measurement system. Afterwards, the impact variables that Acting as an independent variable affecting the energy consumption of the distribution and logistics facilities were categorized into intrinsic and meteorological variables. Lastly, all factors that could affect the energy consumption of the actual distribution and logistics facilities, were classified and presented as guidelines list. By applying the results of this study, it is possible to build a monitoring system at a low cost and high efficiency in a distribution and logistics facility with a complex structure. And by identifying the main independent variables for the measured energy consumption, effectively identifying trends in energy consumption and deriving saving points It is expected to be able to operate the ESS-based infrastructure.

• Korean Journal of Food Science and Technology
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• v.50 no.1
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• pp.105-110
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• 2018

Melittin is the main component of apitoxin (bee venom) that has been reported to have anti-inflammatory and anti-cancer effects. Herein, we demonstrated that inhibition of epithelial-mesenchymal transition (EMT) by melittin causes suppression of cancer cell migration and invasion. Melittin significantly suppressed the epidermal growth factor (EGF)-induced cell migration and invasion in lung cancer cells. Moreover, melittin up-regulated the expression of epithelial marker protein, E-cadherin, and down-regulated the expression of EMT related proteins, vimentin and fibronectin. Mechanistic studies revealed that melittin markedly suppressed the expression of EMT mediated transcription factors, ZEB2, Slug, and Snail. The EGF-induced phosphorylation of AKT, mTOR, P70S6K, and 4EBP1 was also inhibited by melittin, but not that of ERK and JNK. Therefore, the inhibitory effect of melittin on migration and invasion of lung cancer cells may be associated with the inhibition of EMT via blocking of the AKT-mTOR-P70S6K-4EBP1 pathway.

• Toxicological Research
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• v.26 no.4
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• pp.245-252
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• 2010

Epithelial-mesenchymal transition (EMT) is a complex process in which epithelial cells acquire the characteristics of invasive mesenchymal cells. EMT has been implicated in cancer progression and metastasis as well as the formation of many tissues and organs during development. Epithelial cells undergoing EMT lose cell-cell adhesion structures and polarity, and rearrange their cytoskeletons. Several oncogenic pathways such as transforming growth factor (TGF)-$\beta$, Wnt, and Notch signaling pathways, have been shown to induce EMT. These pathways have activated transcription factors including Snail, Slug, and the ZEB family which work as transcriptional repressors of E-cadherin, thereby making epithelial cells motile and resistant to apoptosis. Mounting evidence shows that EMT is associated with cell invasion and tumor progression. In this review, we summarize the characteristic features of EMT, pathways leading to EMT, and the role of EMT in cell invasion. Three topics are addressed in this review: (1) Definition of EMT, (2) Signaling pathways leading to EMT, (3) Role of EMT in cell invasion. Understanding the role of EMT in cell invasion will provide valuable information for establishing strategies to develop anti-metastatic therapeutics which modulate malignant cellular processes mediated by EMT.

• Asian-Australasian Journal of Animal Sciences
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• v.8 no.5
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• pp.489-494
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• 1995

Sero-epidemiological study was carried out to observe the prevalence of brucellosis in 500 slaughtered as well as in 500 healthy animals in Peshawar district of N.W.F.P. All serum samples were subjected to four serological tests i.e. Standard Plate Test (SPT), Standard Tube Test (STT), Rivanol Test (RV) and 2, Mercapto-Ethanol Test (2, ME). The incidence of disease in 500 healthy animals tested by standard plate test, standard tube test, rivanol test and 2, Mercapto-ethanol test, was 2.8%, 1.8%, 1.6% and 1.2% respectively. While the incidence of brucellosis in 500 slaughter animals from Peshawar abattoir was 3.0%, 2.2%, 2.00% and 1.2% by standard plate test, standard tube test, rivanol test and 2, Mercapto-ethanol test The disease prevalence was higher in slaughtered animals as compared to healthy animals. The disease was more common in goats than sheep, also more prevalence in aged female than younger stocks. The efficacy of SPT was found more effective as compared to STT, RV, and 2, ME tests both in slaughtered as well as apparently healthy animals at Peshawar district. Standard Plate test detected 2.9%, Standard Tube test 2.0%, Rivanol test 1.8% and 2, Mercapto-ethanol test detected 1.2% positive cases in slaughtered as well as in healthy animals. So the Standard Plate Test was found to be more reliable, sensitive, and easy to performed.