• KSBB Journal
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• 제26권3호
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• pp.199-205
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• 2011

Using tetrameric ${\beta}$-galactosidase as a model protein, anchoring motives were screened in Bacillus subtilis spore display system. Eleven spore coat proteins were selected considering their expression levels and the location in the spore coat layer. After chromosomal single-copy homologous integration in the amyE site of Bacillus subtilis chromosome, cotE and cotG were chosen as possible spore surface anchoring motives with their higher whole cell ${\beta}$-galactosidase activity. PAGE and Wester blot of extracted fraction of outer layer of purified spore, which express CotE-LacZ or CotG-LacZ fusion verified the existence of exact size of fusion protein and its location in outer coat layer of purified spore. ${\beta}$-galactosidase activity of spore with CotE-LacZ or CotG-LacZ fusion reached its highest value around 16~20 h of culture time in terms of whole cell and purified spore. After intensive spore purification with lysozyme treatment and renografin treatment, spore of BJH135, which expresses CotE-LacZ, retained only 1~2% of its whole cell ${\beta}$-galactosidase activity. Whereas spore of BJH136, which has cotG-lacZ cassette in the chromosome, retained 10~15% of its whole cell ${\beta}$-galactosidase activity, proving minor perturbation of CotG-LacZ, when incorporated in the spore coat layer of Bacillus subtilis compared to CotE-LacZ. Usage of Bacillus subtilis WB700, of which 7 proteases are knocked-out and thereby resulting in 99.7% decrease in protease activity of the host, did not prevent the proteolytic degradation of spore surface expressed CotG-LacZ fusion protein.

• 한국세라믹학회지
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• 제24권5호
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• pp.470-476
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• 1987

The system of 0.1Pb(Mg1/3N2/3)O3-yPbTiO3-zPbZrO3 piezoelectric ceramics was made in the radial model disk and ring dot type filter. The resonant frequency(fr) variations were investigated for 100 hours and the temperature range from 20$^{\circ}C$ to 100$^{\circ}C$. As the results, the morphotropic phase boundary was supposed to be the composition of 45 y 50 and 40 z 45. The best characteristic of againg effect, after 10-hours, was composed of y, z=45. The stabilized characteristic of temperature varition, to become 0.1% up to 100$^{\circ}C$, was composed of y=50 and z=40. The bandwidth was broadened with increasing the electromechanical coefficient(kp), and the filter characteristics was approached ideal mode with increasing mechanical quality factor(Qm). Kp and Qm affected combinationally on filter characteristics.

• 한국정밀공학회지
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• 제20권5호
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• pp.47-56
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• 2003

Geometric cutting-simulation and verification play an important role in detecting NC machining errors in mold & die manufacturing and thereby reducing correcting time & cost on the shop floor. Current researches in the area may be categorized into view-based, solid-based, and discrete vector-based methods mainly depending on workpiece models. Each methodology has its own strengths and weaknesses in terms of computing speed, representation accuracy, and its ability of numerical inspection. The paper proposes a hybrid modeling scheme for workpiece representation with z-map model and discrete vector model, which performs 3-axis and 5-axis cutting-simulation via tool swept surface construction by connecting a sequence of silhouette curves.

• Bulletin of the Korean Chemical Society
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• 제15권6호
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• pp.469-473
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• 1994

The E-and Z-isomers of 1-methyl-l-phenyl-2-neopentylsilene, generated by the sealed tube thermolyses of their anthracene adducts are stereospecifically trapped by trimethylmethoxysilane to give diastereomeric adducts. The temperature dependence of the ratio of the two diastereomers obtained when the silene formed from the pure E-or Z-anthracene adduct was trapped at higher temperatures permitted the determination of an activation energy for the silene isomerization. The activation energies for the E-to Z-and Z-to E-silene isomerization are $45{\pm}$6 and $20{\pm}4$ kcal $mol^{-1}$, respectively. The significance of these values is discussed.

• Nonlinear Functional Analysis and Applications
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• 제28권3호
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• pp.731-751
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• 2023

If p(z) is a polynomial of degree n having all its zeros in |z| ≤ k, k ≥ 1, then for any complex number α with |α| ≥ k, and r ≥ 1, Aziz [1] proved $$\left{{\int}_{0}^{2{\pi}}\,{\left|1+k^ne^{i{\theta}}\right|^r}\,d{\theta}\right}^{\frac{1}{r}}\;{\max\limits_{{\mid}z{\mid}=1}}\,{\mid}p^{\prime}(z){\mid}\,{\geq}\,n\,\left{{\int}_{0}^{2{\pi}}\,{\left|p(e^{i{\theta}})\right|^r\,d{\theta}\right}^{\frac{1}{r}}.$$ In this paper, we obtain an improved extension of the above inequality into polar derivative. Further, we also extend an inequality on polar derivative recently proved by Rather et al. [20] into L^r-norm. Our results not only extend some known polynomial inequalities, but also reduce to some interesting results as particular cases.

• 수산해양기술연구
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• 제56권2호
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• pp.172-182
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• 2020

In order to offer specific information needed to assist in operation of a ship with same type rudder through evaluating the maneuverability of training ship A-Ra with flapped rudder, sea trials based full scale for turning test, zig-zag test with rudder angle 10° and 20°, and spiral test at service condition were carried out on starboard and port sides around Jeju Island according to the standards of maneuverability of IMO. As a result, the angular velocity of port turn was higher than that of starboard turn. Therefore, the size of turning circle was longer on the starboard side. In addition, variation of the transfer due to various factors was more stable than those of the others. In the Z-test results, the mean of 1st and 2nd overshoot angles were 9.8°, 6.3° and 15.3°, 9.2° respectively when the port and starboard was 10°; the 1st overshoot angle were 18°, 13.7° when using 20°. Her maneuverability index T' and K' can be easily determined by using a computer with the data obtained from Z-test where K' and T' are dimensionless constants representing turning ability and responsiveness to the helm, respectively. In the Z-test under flap rudder angle 10°, the obtained K' value covered the range of 2.37-2.87 and T' was 1.74-3.45. Under the flap rudder angle 20°, K' and T' value showed 1.43-1.63, 1.0-1.73, respectively. In the spiral test, the loop width was unstable at +0.3° and -0.5°-0.9° around the midship of flap rudder. As a result, course stability was comparatively good. From the sea trial results, training ship ARA met the present criterion in the standards of maneuverability of IMO.

• 한국식품과학회지
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• 제20권2호
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• pp.256-262
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• 1988

우리나라 콩 중 장려품종(봉의) 및 재래종 품종(KW12, KLS-77005-1 및 B-102)의 수분흡수 특성을 조사하였다. 콩을 $4-98^{\circ}C$에서 침지하였을 때 $40^{\circ}C$ 이하에서는 침지시간 10시간 동안에 평형상태에 도달하지 않았으나, $60^{\circ}C$이상에서는 3시간정도에서 평형상태에 도달하였다. 콩이 수분흡수속도는 102-B>봉의>KLS-77005-1>KW-12의 순이었다. 콩의 수분증가량은 침지온도에 상관없이 침지시간의 평방근과 직선적인 관계를 보였으며, 수분흡수의 기본기작은 확산현상에 기인하였다. 침지온도 $4-20^{\circ}C$에서의 흡수의 활성화에너지 값은 2,0-2.7kcal/mole, 침지온도 $20-60^{\circ}C$에서는 6.4-6.6kcal/mole이었다. 콩을 $4-60^{\circ}C$에 침지하는 동안 일정한 수분함량에 도달하는 침지시간의 대수값과 침지온도와는 직선적인 관계를 보였으며, 콩의 수화도가 증가할수록 z-값은 감소하였다. 수분함량 50%에 도달하는 z-값은 시료콩 모두 $50^{\circ}C$정도 이었다.

• 한국재료학회지
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• 제11권3호
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• pp.171-177
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• 2001

급냉응고방식으로 제조한 비정질 Z $r_{62-x}$N $i_{10}$C $u_{20}$A $l_{8}$ $Ti_{x}$ (x=3, 6, 9at%) 합금을 사용하여 열적, 기계적 성질을 조사하였다. 시효온도에 따른 결정화 거동은 Ti 3at%에서는 비정질$\longrightarrow$비정질+Z $r_2$A $l_3$+Zr+(Ni,Ti)$\longrightarrow$Z $r_2$Cu+Al+(Ni,Ti)의 결정화 거동을 나타내었으며, Ti 6at%에서는 비정질$\longrightarrow$비정질+Al$\longrightarrow$A $l_2$Ti+NiZr+CuTi, Ti 9at%에서는 비정질$\longrightarrow$비정질+Zr+Al$\longrightarrow$Zr+A $l_2$Zr+Al $Ti_3$+CuTi의 결정화 거동을 보였다. 시효온도가 증가할수록 비정질 모상에 석출상의 체적율( $V_{f}$ )이 증가하고 그에 따라 비커스 경도 ( $H_{v}$ )간이 증가하였다. 파괴인장강도($\sigma_{f}$ )는 $V_{f}$ 의 증가에 따라 증가하다가 Z $r_{59}$A $l_{10}$N $i_{20}$C $u_{8}$ $Ti_3$은 $V_{f}$ =38%에서 1219MPa의 최대값을 보이고, Z $r_{56}$A $l_{10}$N $i_{20}$C $u_{8}$ $Ti_{6}$은 $V_{f}$ =2%에서 1203MPa의 최대값을 보이고, Z $r_{53}$A $l_{10}$N $i_{20}$C $u_{8}$ $Ti_{9}$ $V_{f}$ =5%에서 1350MPa의 최대값을 나타낸 후 그 이상의 $V_{f}$ 에서는 급격히 감소하였다. $\sigma_{f}$ 가 급격히 감소하는 $V_{f}$ 와 연성 파면에서 취성파면으로 천이되는 $V_{f}$ 가 일치하였다.f/가 일치하였다.

• 한국재난정보학회 논문집
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• 제12권1호
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• pp.98-104
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• 2016

본 연구에서는 PVC 오수받이를 대상으로 상용 프로그램인 FEM LAB(ver. 3.2)을 이용하여 여러 시공 환경조건에서 오수받이 내부에 설치된 악취방지용 Z형 트랩 내부에 잔류하고 있는 물의 겨울철 동결여부를 평가하였다. 시뮬레이션 결과 외부온도 $-20^{\circ}C$가 42일간 지속되더라도 토양이 건조한 상태라면 Z형 트랩에 남아 있는 물이 동결되지 않음을 알 수 있다. 토양이 습윤 상태일 경우 약 14일 정도, 토양이 일반상태의 경우 약 17일 정도가 지나면 Z형 트랩이 위치한 지면으로부터 60cm 지점이 Z형 트랩 내에 동결될 가능성이 있으나, 토양이 건조한 상태에 있을 경우에는 42일이 지나도 지면으로부터 60cm 지점은 영상의 온도를 유지하는 것으로 보아 Z형 트랩 내에 있는 물이 동결되지 않는다는 것을 알 수 있다.

• 조명전기설비학회논문지
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• 제20권8호
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• pp.22-29
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• 2006

IEC 규격을 기반으로 최근에 개정된 KS 규격에 따라 등전위 접지시스템이 중요하게 자리매김 하였으며, 전원시스템의 안정성을 위해 서지보호소자의 사용이 급격히 증가하고 있다. 내재된 비선형 저항성분으로 뛰어난 V-I 특성을 가지는 $Z_nO$ 바리스터는 서지전압을 제한하여 서지전류로 환류시키기 위해 전원용 보호기로 주로 사용되고 있다. 이러한 $Z_nO$ 바리스터는 교류 전원선에 접속하기 위해서 몇 가지 회로조합 형태로 구성되어 사용되는데, 사용자는 바리스터를 직렬 혹은 병렬로 조합하여 사용함에 있어서 안전에 직접적으로 관련된 기능이나 열적 안정성을 포함한 많은 것들을 고려하여야 한다. 본 논문에서는 40[kA]의 전류용량을 가지는 단일 바리스터 소자와 직렬 혹은 병렬 회로조합의 바리스터에 대하여 잔류전압, 방전전류, 누설전류, 표면온도를 측정하여 각각의 조합형태에 따라 안정성을 비교하였다.