• 동의생리병리학회지
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• 제16권2호
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• pp.359-364
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• 2002

The present study examined the effects of water extract of Hwangruynhaedoktang plus Yukmijihwangtang add Chogudeung (HYC) on the blood pressure and renal function in spontaneously hypertensive rats (SHR). The HYC water extract blocked increase of systolic blood pressure (SBP) in SHR. The urinary volume (UV), sodium (UNaV), potassium (UkV), and chloride (UclV) excretions were significantly increased in SHR administered with high dose of water extract of HYC, but urinary osmolality was not changed. The creatinine clearance (Ccr), which is index of glomerular filtration rate (GFR), was increased by administration of HYC, while solute-free water reabsorption (TcH2O) was not changed. Taken together, these results suggest that the depressor effects of HYC, at least in part, caused by diuretic effect due to increase of glomerular filtration rate.

• 동의생리병리학회지
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• 제17권2호
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• pp.436-442
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• 2003

In order to examine the antioxidant actions of YMJHT, the study was done through measurement of parameters such as LPO(lipidperoxidation), GSH(glutathione), SOD(superoxidation dismutase), catalase, GOT, GPT, ALP, the results were obtained as follows: For the weight changes, in the testis the group given YMJHT showed significant increase compared to the control group. In the left cerebrum, the group given YMJHT showed significant increase compared to the control group on the activities of SOD, catalase. In the right cerebrum, the group given YMJHT showed significant decrease on the content of LPO and showed significant increase on the activity of catalase. In the cerebellum, the group given YMJHT showed significant decrease on the content of LPO and showed significant increase on the activities of SOD, catalase. In the liver, the group given YMJHT showed significant decrease on the content of GSH and showed significant increase on the activity of SOD. In the kidney, the group given YMJHT showed significant decrease on the contents of GSH, GSH and showed significant increase on the activities of SOD, catalase. In the testis, the group given YMJHT showed significant decrease on the contents of LPO, GSH and showed significant increase on the activity of SOD. From above results, the antioxidant action of YMJHT is effective. And it is expected to be necessary to the study of the mechanism in the antioxidant of YMJHT.

• 대한한방내과학회지
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• 제28권4호
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• pp.919-928
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• 2007

Objectives : This study was designed to research the effects of oriental treatment on dyskinesia with lower limbs caused by Parkinson's Disease. Methods : We prescribed Yukmijihwang-tang (六味地黃湯) and Jaumganghwa-tang (滋陰降火湯) and treated acupuncture through Shinjungkyuk (腎正格) every day. It was used to treat the Shinjeonghyuson (腎精虧損) symptoms. We analyzed the frequency of dyskinesia over one night at intervals of three days and measured the score of 'Unified Parkinson's Disease Rating Scale' every week to evaluate the entire symptom's improvement. Results and Conclusion : He had been treated with western medicine to improve the dyskinesia symptoms, but couldn't get a noticeable result. Having oriental treatment for 43 days, dyskinesia in the lower limbs mostly disappeared and all symptoms of Parkinson's disease (tremor, rigidity, dysphagia, hallucination, anorexia, etc.) improved significantly.

• 동의생리병리학회지
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• 제16권5호
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• pp.976-988
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• 2002

The aim of this study was to examine the memory and attention enhancement effect of YMG-1 and YMG-2, which are modified herbal extracts from Yukmijihwang-tang (YMJ). YMJ, composing six herbal medicine, has been used for restoring the normal functions of the body to consolidate the constitution, nourishing and invigorating the kidney functions for hundreds years in Asian countries. A series of studies reported that YMJ and its components enhance memory retention, protects neuronal cell from reactive oxygen attack and boost immune activities. Recently the microarray analysis suggested that YMG-1 protects neurodegeneration through modulating various neuron specific genes. A total of 55 subjects were divided into three groups according to the treatment of YMG-1 (n=20), YMG-2 (n=20) and control (C; n=15) groups. Before treatments, all of subjects were subjected to the assessments on neuropsychological tests of K-WAIS test, Rey-Kim memory test, and psychophysiological test of Event-Related Potential (ERP) during auditory oddball task and repeated word recognition task. They were repeatedly assessed with the same methods after drug treatment for 6 weeks. Although no significant effect of drug was found in Rey-Kim memory test, a significant interaction (P = .010, P < 0.05) between YMG-2 and C groups was identified in the scores digit span and block design, which are the subscales of K-WAIS. The very similar but marginal interaction (P = .064) between YMG-1 and C groups was found too. In ERP analysis, only YMG-1 group showed decreasing tendency of P300 latency during oddball task while the others tended to increase, and it caused significant interaction between session and group (p= .004). This result implies the enhancement of cognitive function in due to consideration of relationship between P300 latency and the speed of information processing. However, no evidence which could demonstrate the significant drug effect was found in neither amplitude or latency. These results come together suggest that YMG-1, 2 may enhance the attention, resulting in enhancement of memory processing. For elucidating detailed mechanism of YMG on learning and memory, the further studies are necessary.

• 동의생리병리학회지
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• 제16권5호
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• pp.1025-1034
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• 2002

The herbal extract (YMT_02) is a modified herbal extracts from Yukmijihwang-tang (YMJ) to promote memory-enhancing. The YMJ extracts has been widely used as an anti-aging herbal medicine for hundred years in Asian countries. The purpose of this study is to; 1) quantitatively evaluate the memory-enhancing effect of YMT_02 by behavior task, 2) identify candidate genes responsible for enhancing memory by cDNA microarray and 3) assess the anti-oxidant effect of YMT_02 on PC12 cell. Memory retention abilities are addressed by passive avoidance task with Sprague-Dawley (SD) male rat. Before the training session, the rats are subdivided into four groups and administrated with YMT_02, Ginkgo biloba, Soya lecithin and normal saline for 10 days. The retention test was performed. 24 hours after the training session. The retention time of the YMT_02 group was significantly (p<0.05) delayed (～100%), whereas Ginkgo biloba and Soya lecithin treatment delayed 20% and 10% respectively. The hippocampi of YMT_02 and control group were dissected and mANA was further purified. After synthesizing cDNA using oligo-dT primer, the cDNA were applied to Incyte rat GEMTM 2 cDNA microarray. The microarray results show that prealbumin(transthyretin), phosphotidylethanolamine N-methyltransferase, and PEP-19 are expressed abundantly in the YMT_02 treated group. Especially, PEP-19 is a neuron-specific protein, which inhibits apoptotic processes in neuronal cell. On the other hand, transcripts of RAB15, glutamate receptor subunit 2 and CDK108 are abundant in control group. Besides, neuronal genes involved in neuronal death or neurodegeneration such as neuronal-pentraxin and spectrin are abundantly expressed in control group. Additionally, the YMT_02 shows an anti oxidative effect in the PC12 cell. The list of differentially expressed genes may implicate further insight on the action and mechanism behind the memory-enhancing effect of herbal extracts YMT_02, for example, anti-apoptotic, anti-oxidative, and neuroprotective effects.