• Title/Summary/Keyword: Young-Hoon Kim

Search Result 9,791, Processing Time 0.078 seconds

Cloning of $\beta$-glucosidase gene from Cellulomonas sp. into E.coli

  • Kim, Ha-Geun;Kim, Hoon;Park, Moo-Young
    • Proceedings of the Korean Society for Applied Microbiology Conference
    • /
    • 1986.12a
    • /
    • pp.525.1-525
    • /
    • 1986
  • To clone ${\beta}$-glucosidase gene from Cellulomonas sp. a gene library was constructed using E. coli JM83 pUC9. Among 2,500 pseudotransformants obtained, 20 clones developed yellow color on the p-nitrophenyl- -D-glucopyranoside filter paper These 20 clones were classified into three groups based on the results of activity staining using nondenaturating polyacrylamide gel electrophoresis and restriction enzyme digestions. Among the three groups, only one group containing pCEl plasmid has specificity for cellobiose.

  • PDF

Regio- & Stereoselective Synthetic Method for Polyhydroxyamines using Chlorosulfonyl lsocyanate

  • Kim, In-Su;Lim, Seung-Yong;Kim, Ji-Duck;Jung, Young-Hoon
    • Proceedings of the PSK Conference
    • /
    • 2002.10a
    • /
    • pp.341.3-342
    • /
    • 2002
  • The interest in polyhydroxyamines is based in their biological activity as enzyme inhibitors. and as starting materials in the synthesis of more complex compounds. Polyhydroxyamines is that amine group is continuing structurally with hydroxy groups and has become important target of the synthesis strategy because of the chirality control of amine group and each hydroxy groups. (omitted)

  • PDF