• 수산해양교육연구
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• 제27권5호
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• pp.1499-1507
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• 2015

The aim of this study is to research on the recognitions and preferences of Puffer fish consumers with the recently increasing well-being trend. To carry out this research, the main analysis was focused on two specific points: how much consumers perceive swellfish and which kinds of Puffer fish foods are preferred by consumers. Although the consumption of Puffer fish is gradually expending, at the same time, there are also numerous obstructive elements in consuming swellfish. In this regard, this test on Puffer fish food will be able to contribute to show an outlook for the Puffer fish food market in the future and to promote consumption of Puffer fish as well. Methodologically, a statistical research was adopted to find out how people understand Puffer fish and the patterns of their choices and intensive examinations were conducted throughout the collected questionnaire. For a more effective outcome, it was necessary to divide into two groups, the Nation's capital area with Seoul as a center, the most densely populated area and Busan, the biggest marine products consumption area, examining the inclination to consume with regions. In the concrete, the detailed research survey on the Puffer fish were performed with the opinions of the five hundred people from capital area and the five hundred people from Busan area. The difference and common features of consumer's recognition about Puffer fish food were founded through cross analysis according to age, gender, regions, and income. In conclusion, this research showed the difference tastes and recognition standards toward Puffer fish among consumers from Seoul and Busan areas. If it is possible to apply this result to the efforts of improving supplier's understanding about Puffer fish consumers' features and cultivating new Puffer fish items, it could contribute to the further consumption of Puffer fish food in the long term point of view.

• 혜화의학회지
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• 제28권2호
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• pp.1-11
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• 2019

Objective: The aim of this study is to analyze the previously published research articles related to puffer fish toxin focusing on tumor. Method: Literatures were searched in PubMed database, published since 2000, using the keyword; Puffer fish, Fugu and tetrodotoxin (TTX) with cancer or tumor. Research papers were classified by year, country, study model, used material, kind of tumor and study subject. Finally, a total of 41 studies were analyzed in this study. Results: From 2000 to 2018, the most abundant papers were published in 2009 (6 studies) and almost half of the papers were studied in United Kingdom (20 studies). The 39 studies used TTX purified from puffer fish while 2 studies used crude extract of skin and gonad of puffer fish. The most used target cell line was prostate cancer (15 studies), and the next was breast cancer (14 studies). The study methods were classified into 4 clinical studies, 2 animal studies and 35 cell-based studies. Conclusions: Our results show that the overview of cancer-related studies using puffer fish poison. This information would be helpful for the puffer fish-derived drug researches in the future.

• 한국발생생물학회지:발생과생식
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• 제21권2호
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• pp.181-191
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• 2017

A comparison of the growth, hematological values, fatty acids, and gonadal and growth hormonal changes of river puffer, Takifugu obscurus, tiger puffer, T. rubripes, their hybrids (river puffer ${\times}$ tiger puffer) and hybrid triploids was performed during 3 months of their early growth period. Several features were observed during these 3 months: hybrids showed the highest levels of specific growth rate, 1.48%; hybrid triploids showed the smallest change in viscera fat (P<0.05), but GSI was not significantly different among groups (P>0.05). Considering hematological parameters, hybrid triploids had increased mean corpuscular volume and mean corpuscular hemoglobin (P<0.05), but other parameters were not significantly different between groups (P>0.05). With respect to fatty acids, puffer fish, hybrids and hybrid triploids contained fatty acids such as SFAs, MUFAs, n-3 PUFAs and n-6 PUFAs. There were significantly different amounts of total fatty acids between groups (P<0.05), however, rates of changes in fatty acids did not differ significantly between groups (P>0.05). Gonadal hormone (estradiol and testosterone) changes in the river puffer and tiger puffer were significantly higher than that observed in hybrids and hybrid triploids. The hybrids and tiger puffers had higher amounts of growth hormone (thyroid stimulating hormone and thyroxine) than the hybrid triploids and river puffers (P<0.05).

• 한국발생생물학회지:발생과생식
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• 제20권1호
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• pp.23-29
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• 2016

In fish, light (photoperiod, intensity and spectra) is main regulator in many physiological actions including growth. We investigate the effect of light spectra on the somatic growth and growth-related gene expression in the rearing tiger puffer. Fish was reared under different light spectra (blue, green and red) for 8 weeks. Fish body weight and total length were promoted when reared under green light condition than red light condition. Expression of somatostatins (ss1 and ss2) in brain were showed higher expression under red light condition than green light condition. The ss3 mRNA was observed only higher expression in blue light condition. Expression of growth hormone (gh) in pituitary was detected no different levels between experimental groups. However, the fish of green light condition group was showed more high weight gain and feed efficiency than other light condition groups. Our present results suggest that somatic growth of tiger puffer is induced under green light condition because of inhibiting ss mRNA expression in brain by effect of green wavelength.

• 조명전기설비학회논문지
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• 제25권12호
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• pp.74-81
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• 2011

A computational approach was developed to understand about the arc quenching process in a gas circuit breaker(GCB). This approach is a program to analyze the gas flow in the breaker. The arc is processed at the same time. The program was used the so-called FLIC method for gas analysis techniques. It was referenced that the arc is interpreted the 'a Simplified Enthalpy Flow Arc Model'. In order to validate about the results of the program, a Auto Puffer GCB was chosen as the test subject. Because, the breaker is the one that arc current is interrupted by using the arc heating. And also, the current interrupting capability can be obtained only owing to the positive utilization(auto puffer) of the clogging phenomenon, without other puffer actions. In this paper, it has been realized that the entire arc quenching process is computerized, which is based on the self-flow current interruption by the auto puffer action. This program, which was verified through experiments, produced good results.

• 한국수산과학회지
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• 제31권3호
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• pp.353-358
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• 1998

Experiments were performed to find out the physico-chemical properties of milt, and morphological changes of cryopreserved spermatozoa in tiger puffer, Takifugu rubripes. The average number of sperm and spermatocrit in milt stripped were $9.81{\pm}0.34{\times}10^{10}/m{\ell}$ and $97.8{\pm}0.8$, respectively. While total lipid concentration from seminal fluid was higher than that from sperm, total protein concentration from sperm was higher than that from seminal fluid, Na and K concentrations in sperm and those in seminal fluid were similar each other, However, glucose from sperm and seminal fluid were not detectable. Spermatozoon of tiger puffer was consisted of head, middle Piece and tail. Size of head showing horseshoe shape was $0.65{\pm}0.10{\mu}m$ in diameter and $1.35{\pm}0.30{\mu}m$ in length. The head fully containing chromatin did not have acrosome. Mitochondrion in middle piece was $0.2{\mu}m$ in average diameter and flagellum showed 9+2 structure. A few of cryopreserved spermatozoa showed morphologically loose or swollen plasma membranes.

• 한국양식학회지
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• 제9권4호
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• pp.353-361
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• 1996

자주복 유어의 필수지방산 요구 및 사료중의 적정함량을 규명하기 위하여, 평균체중 3.45g의 유어를 사용하여 사육실험을 실시하였다. 8주간의 사육실험의 결과, 성장은 실험개시 4주부터 실험구들 사이에 차이가 보여 실험 종료시에는 $0.5\~1\%$ n-3 HUFA 실험구와 $0.5\%$ DHA 실험구가 다른 실험구에 비교하여 우세한 성장을 보였다(P<0.05). 또한, EPA, DHA 및 n-3 HUFA 실험구 사이에 효율성의 비교에서 EPA$1\%$ EPA실험구는 사료중에 $0.5\%$에서 $1\%$로 지방산을 증량시킴에 의해 첨가효과가 인정되었으나, DHA와 n-3 HUFA실험구는 EPA실험구와 상이하게 $1\%$ DHA실험구에서 급격한 성장의 저하와 $1\%$ n-3 HUFA 실험구에서는 성장의 정체가 나타났다. 실험기간동안에 생잔률은 $73\~93\%$의 범위로 지방산과의 관련성은 보이지 않았다. 사료효율은 n-3 HUFA실험구와 $0.5\%$ DHA실험구에서 높았고, $1\%$ LNA실험구는 $0.5\~1\%$ EPA실험구와 $0.3\%$ DHA실험구와의 차이가 거의 없었다. 이상의 결과로 부터, 유어기의 자주복은 EPA나 DHA등의 n-3 HUFA를 필수지방산으로 요구함이 사료되며, 사료중의 $0.5\%$ 정도의 HUFA 또는 $0.5\%$ DHA가 적정함량으로 추측된다.

• 한국수산과학회지
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• 제32권3호
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• pp.243-246
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• 1999

황복 (Takifugu obscurus)의 정자보존을 위한 기초자료를 얻고자, 정액의 특성을 파악하고 정자의 냉장보존 조건에 관한 연구를 수행하였다. 정액의 ml당 정자수는 $1.13\pm0.34\times10^{10}/ml$이었고 spermatocrit는 64.8$\pm$1.4였으며, 정장의 삼투질농도는 266$\pm$2 mOsm/kg이었다. 총 단백질 함량과 총 지질 함량은 정장에 비해 정자에서 높았으며, glucose는 검출되지 않았다. $Ca^{2+}$과 $Na^{+}$ 농도는 정자에 비해 정장에서 높았고, $Mg^{2+}$과 $K^{+}$은 정장에 비해 정자에서 높은 값을 보였다. 황복 정자를 여러 가지 희석액을 이용하여 16 일간 $0\pm0.5^{\circ}C$에서 냉장보존 하였을 때, 수정률은 $0\~0.7\%$로 보존 효과가 저조한 것으로 나타났다.

• 한국발생생물학회지:발생과생식
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• 제22권2호
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• pp.175-182
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• 2018

In order to examine the effects of four different light spectra (white, red, green, and blue) on the oocyte maturation, the change of reproductive parameters, via brain-pituitary-gonad (BPG) axis in grass puffer, were investigated. After exposure four different light spectra for 7 weeks, the abundance of gonadotropin-releasing hormone (GnRH) mRNA which is a type of seabream (sbGnRH) and two different subunit of gonadotropin hormones mRNAs, follicle-stimulating hormone ($fsh{\beta}$) mRNA and luteinizing hormone ($lh{\beta}$) mRNA, were analyzed in the brain and pituitary. Histological analysis showed that the mature oocyte ratio in the green spectrum was higher than other light spectra-exposed groups. Gonadosomatic index (GSI) and oocyte developmental stage were also investigated in the gonad based on histological observations. GSI value with the presence of yolk stage oocytes was significantly increased in the green spectrum-exposed group when compared to that of the other light-exposed groups (white, red, and blue) (p<0.05). The abundances of sbGnRH mRNA and $fsh{\beta}$ mRNA in the green spectrum-exposed group were also significant higher than those of the other light spectra-exposed groups (p<0.05). These results indicate that the maturation of oocyte in grass puffer can be accelerated by exposure to the spectrum of green. To better understand the molecular mechanism for the maturation of oocyte in grass puffer, further study examining the relationship between oocyte development and its related genes is required.

• 한국양식학회지
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• 제12권4호
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• pp.255-266
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• 1999

The early development, growth, and morphological changes of Tiger puffer Takifugu rubripes are described on the basis of a seres of a series of reared specimens. Detailed o수 the early developmental stages are illusrtated withe special reference to morphological transformation. Egg and sperm of puffer fish Takifugu rubripes were obtained from mature adults under natural conditions, ferilized artificially and incubated in the laboratory. The incubation period of fertilizd eggs was 160 to 180 hours at a temperature of $15.1~ 18.0^{\circ}C$. Larvae were fed successively with rotifer, Artemia nauplii and artificial food for 90 days. The mean total length of newly-hatched larvae was about 2.8mm. Mouth opening occurred on the 1- day yolk-sac larvae and initial feeding was observed on th 4-5 days after hatching . The morphological transitions from the larvae to juvenile and juvenile and juvenile to young stages occurred when fish reached about 10mm in total length(about 30 days after hatching) and about 32mm in total length(about 60days after hatching) , respectively. The coefficient of variation in total length distribution increased with growth. Following the appearance of its peak at the size of about 18mm in total length, the coefficient value declined. Many changes in proportion of the body parts to total length were observed at about 9~10mm and 30~33mm in total length, corresponding to the transformations from larvae to juvenile and from juvenile to young, respectively. Also one big morphological change was observed at about 18mm in total length that divide the juvenile stage into two sub-stages.