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White Spot Syndrome Virus(WSSV) and Hepatopancreatic Parvovirus (HPV)Infection of Penaeus chinensis in Korea

  • K. V. Rajendran;S. J. Jung;Kim, Y. J.;Kim, S. R.;M. J. Oh
    • Proceedings of the Korean Society of Fisheries Technology Conference
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    • 2001.05a
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    • pp.521-523
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    • 2001
  • White spot syndrome virus (WSSV) causes the most serious epizootic in cultured penaeid shrimp. The epizootic started in 1992, and spread through east and south east Asia and into other shrimp growing countries of the region. WSSV has also been reported from Penaeus chinensis cultured in Korean peninsula (Park et al., 1998; Kim et al., 1999). However, this paper deals with the WSSV infection in cultured and wild P. chinensis, purification and characterization of the pathogen, and the occurrence of other concomitant infection. (omitted)

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Anti-cell Adhesion Effect of PLM-f74 with U937 Cell from Hallophilic Enterobacteria and Identification of Strain

  • Lim, Jong-Kwon;Seo, Hyo-Jin;Shin, Jin-Hyuk;Lee, Se-Young;Kim, Min-Yong;Kim, Jong-Deog
    • 한국생물공학회:학술대회논문집
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    • 2005.10a
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    • pp.406-411
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    • 2005
  • Fermented materials with enterobacteria isolated from fusiform fish, have strong anti-angiogenesis effect and anti-cell adhesion effect. PLM-f74 got from 74th fraction of size exclusion chromatography from fermented material, showed strong anti-cell adhesion effect between HUVECs and U937 monocytic cell. Adhesion of U937 cell to HUVEC stimulated with IL-1b was clearly inhibited by PLM-f74 in a dose-dependent manner by 12.1, 21.2, 50.9, and 78.2%, when U937 cells treated with each of the PLM-f74 and stimulated with PMA (100 mg/L) was added onto untreated and unstimulated HUVECs, adhesion was observed by 15.8, 31.9, 70.8, and 102%, when both cell types were pretreated with PLM-f74, the adhesion was prominently decreased by 83.7, 99.2, 110, and 120.8%, with 0.74, 3.7, 7.4, and 18.5ug/mL of PLM-f74, respectively. PLM-f74, also, reduced IL-1-stimulated HUVEC expression of adhesion molecules, VCAM-1, ICAM-1, and E-selectin dose-dependently by ELISA method.

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Purification and Characterization of Protease from the Hepatopancreas of Shrimp, Penaeus orientalis

  • Oh Eun-Sil;Kim Doo-Sang;Choi Sung-Mi;Kim Jeong-Han;Pyeun Jae-Hyeung;Cho Deuk-Moon;Kim Hyeung-Rak
    • Fisheries and Aquatic Sciences
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    • v.2 no.2
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    • pp.218-225
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    • 1999
  • A protease without tryptic and chymotryptic activities was purified from the hepatopancreas of shrimp, Penaeus orientalis, using Q-Sepharose ionic exchange, benzamidine Sepharose-6B affinity, Mono-Q, and gel chromatography. Molecular weight (M.W.) of the protease was estimated to be 27kDa by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS­PAGE). The amino acid composition of the protease was different from that of protease from P. japonicus or trypsin from P. orientalis. The protease was completely inhibited by benzamidine, $N\alpha-p-tosyl-L-lysine$ chloromethyl ketone (TLCK), and phenylmethylsulfonyl fluoride (PMSF) and was not affected by leupeptin, pepstatin, N-tosyl-L-phenylalanine chloromethyl ketone (TPCK), iodoacetate, and ethylenediamine tetra acetate (EDTA). The enzyme did not have any activity against Na-benzoyl-DL-arginine p-nitroanilide (BAPNA) or N-benzoyl-L-tyrosine ethyl ester (BTEE) which are specific substrates of trypsin and chymotrypsin, respectively. However, the protease showed hydrolytic activity for a carboxyl terminal of Tyr, Trp, Phe, Glu, and Cys.

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The Relationship between Physiological Activity and Cell Number in Dolsan Leaf Mustard Kimchi (Brassica juncea)

  • Park, You-Young;Yoo, Eun-Jeong;Lim, Hyun-Soo;Kang, Dong-Soo;Naoyuki Nishizawa;Park, Myeong-Rak
    • Preventive Nutrition and Food Science
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    • v.6 no.2
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    • pp.117-121
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    • 2001
  • Changes in antioxidative activity and Angiotensin converting enzyme(ACE) inhibitory activity in juice prepared from Dolsan leaf mustard kimchi (DLMK) ar various fermentation temperatures were investigated. Antioxidative activity of juice from optimally ripened DLMK at 20 and 3$0^{\circ}C$ showed 80 and 83%, respectively. Juice from 10-day fermented DLMK at 3$0^{\circ}C$ showed 62% inhibitory activity against the ACE. In the juice fermented DLMK at 2$0^{\circ}C$~3$0^{\circ}C$, physiological activity was higher than that of the 4~1$0^{\circ}C$. In particular, optimally ripened DLMK at 3$0^{\circ}C$ showed the highest physiological activity. The physiological activity in DLMK juice at the fermentation period increased significantly with an increase in the growth of microbes. Consequently, a maximum physiological activity was shown at the maximum cell number. These results suggest that the microorganisms in DLMK juice would play an important role in the physiological activity.

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Obstacle avoidance method in the UAV

  • Bae, Young-Chul;Kim, Chun-Suk;Kim, Yig-On
    • Proceedings of the Korean Institute of Intelligent Systems Conference
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    • 2004.10a
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    • pp.7-10
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    • 2004
  • In this paper, we propose a method to avoid obstacles that have unstable limit cycles in a chaos trajectory surface. We assume all obstacles in the chaos trajectory surface have a Van der Pol equation with an unstable limit cycle. When a chaos UAVs meet an obstacle in an Arnold equation or Chua's equation trajectory, the obstacle reflects the UAV

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Target searching method in the UAV

  • Bae, Young-Chul;Kim, Chun-Suk;Kim, Yi-Gon
    • Proceedings of the Korean Institute of Intelligent Systems Conference
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    • 2004.10a
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    • pp.11-14
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    • 2004
  • In this paper, we propose a method to target searching method that have unstable limit cycles in a chaos trajectory surface. We assume all targets in the chaos trajectory surface have a Van der Pol equation with an unstable limit cycle When a chaos UAV meet the target in the Arnold equation, Chua's equation trajectory, the target absorptive the UAV

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The post-embryonic developments of Pseudodiaptomus koreanus Soh and Suh(Copepoda, Calanoida)

  • Moon, Seung-Yong;Soh, Ho-Young;Park, Sang-Duk
    • Proceedings of the Korean Society of Fisheries Technology Conference
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    • 2003.05a
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    • pp.270-271
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    • 2003
  • The genus of Pseudodiaptomus Herrick, 1884 presently comprises 74 species worldwide extending freshwater to hypersaline water(Walter, 2002). Of these, the post-embryonic developments of their naupliar and copepodid stages have been reported in only eight species: P euryhalinus, P. cornatus, P. richardi inequalis, P. marinus, P. binghami, P. poplesia, P. aurivilli and P. hessei. (omitted)

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The Hardware Implementation of Chaotic Robot (카오스 로봇의 하드웨어 구현)

  • Bae Youngchul;Kim Yi-Gon;Kim Cheonsuk;;Koo Youngduk
    • Proceedings of the Korean Institute of Intelligent Systems Conference
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    • 2005.11a
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    • pp.413-416
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    • 2005
  • 본 본문에서는 여러 가지 카오스 방정식을 자율 이동 로봇에 내장할 수 있는 카오스 이동 로봇의 하드웨어를 구현하였다. 이 카오스 로봇은 로봇 주행이 다양한 곡면의 카오스 궤적을 가지고 주행 또는 탐색할 수 있도록 여러 종류의 카오스 회로 즉 Chua's 회로, Lorenz 회로, 하이퍼카오스 회로 등을 카오스 로봇에 내장하도록 설계되어 있도록 설계되어 있다.

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Coastal Topography and Shoreline Change in Gohyun Bay, Geojedo (거제 고현만 주변해역의 지형 및 해안선 변화 특성)

  • Kim Jong-Kyu;Kim Myong-Won;Lee Moon-Ock;Lee Yeon-Gyu
    • Proceedings of the Korea Committee for Ocean Resources and Engineering Conference
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    • 2004.11a
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    • pp.137-142
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    • 2004
  • There has been considerable controversy over the change of coastal topography and shoreline by coastal erosion in Gohyun Bay, Geojedo. In this study, we analyzed aerial photographs and surveyed coastal topography and shoreline. Changes between years were identified using a GIS overlay analysis and field surveys with DGPS, Total Station and Echo Sounder. As a result, we were able to identify changes by coastal erosion in the area of Gohyun Bay, Geojedo.

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