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Development of New Method for Antioxidant Capacity with ORP-pH System

  • Lee Se Yeong;Kim Eun Ok;Seo Hyo Jin;Kim Min Yong;Kim Jong Deog
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.9 no.6
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    • pp.514-518
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    • 2004
  • Many methods are used in the measurement of antioxidative capacity. These meth­ods require very complex procedures and pretreatment. Our suggestions for research will be simple and accurate methods for obtaining many kinds of samples, especially colored samples such as natural product extracts for measuring antioxidative capacities. For oxidation-reduction potential (ORP) system value, we examined the relationships between the ORP-pH system and the ORAC, FRAP methods. To evaluate ORP System value, we calculated the absolute slope/intercept from the linear regression of each standard material at different concentrations and ORP-pH system, and compared the correlations with ORAC and FRAP values.

Anti-Angiogenesis Effect and Cytotoxicity of Enterobacteria Isolated from Fusiform Fish

  • Lim, Jong-Kwon;Song, Min-Gyu;Shin, Jin-Hyuk;Lee, Se-Young;Kim, Jong-Deog
    • 한국생물공학회:학술대회논문집
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    • 2005.04a
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    • pp.158-162
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    • 2005
  • Enterobacteria, named ${\lambda}-bacteria$ isolated from fusiform fish, have strong anti-angiogenesis effect. ${\lambda}-28$ species bore higher anti-angiogenesis effect. Cultured liquid was performed salting out, dialysed and freezed dried. This sample was executed size exclusion chromatography with fraction collector. Anti-angiogenesis, cytotoxicity, and SDS-PAGE were carried out with fraction number. ${\lambda}-28$ species was lower toxicity against HUVECs and effective band was conformed with SDS-PAGE.

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