• KOREAN JOURNAL OF CROP SCIENCE
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• 제46권2호
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• pp.145-149
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• 2001

This study was performed to evaluate the growth and nodulation characters of hypernodulating soy-bean mutant, SS2-2, and to know the growth and yield performance of the mutant in infertile soil. Soil fertility was adjusted by mixing the different ratios of soil components including clay, river sand, and horticultural bed, which resulted in fertile and infertile soil. Dry weight, nitrogen concentration, and leaf nitrate reductase of each plant were measured around V6 stage (47 days after planting) and around R3 stage (82 days after planting). There were significant effects of soil fertility and soybean genotype on the total dry weights including root, nodule, stem, leaf, and pod dry weight at V6 and R3 stages. Total dry weight of hypernodulating mutant, SS2-2, was clearly less than that of its wild type, Sinpaldalkong 2. However, nodule development on the roots of SS2-2 was much greater than that of Sinpaldalkong 2, regardless of soil fertility. Though SS2-2 was smaller in plant size than Sinpaldalkong 2, genotypic difference in total nitrogen content was not significant at both V6 and R3 stages because SS2-2 fixed more nitrogen biologically than its wild type in the root nodule. The SS2-2 mutant showed lower plant yield in both infertile and fertile soil. The SS2-2 contained more crude seed protein than Sinpaldalkong 2, and was characterized with reduced top and root growth.

• Journal of Microbiology and Biotechnology
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• 제16권8호
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• pp.1174-1179
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• 2006

The wild-type Corynebacterium glutamicum ATIC 13032 and Corynebacterium glutamicum ATTC 13032 lysC S301Y, exhibiting a deregulated aspartokinase, were compared concerning growth, lysine production, and intracellular carbon fluxes. Both strains differ by only one single nucleotide over the whole genome. In comparison to the wild-type, the mutant showed significant production of lysine with a molar yield of 0.087 mol (mol glucose$^{-1}$) whereas the biomass yield was reduced. The deregulation of aspartokinase further led to a global rearrangement of carbon flux throughout the whole central metabolism. This involved an increased flux through the pentose phosphate pathway (PPP) and an increased flux through anaplerosis. Because of this, the mutant revealed an enhanced supply of NADPH and oxaloacetate required for lysine biosynthesis. Additionally, the lumped flux through phosphoenolpyruvate carboxykinase and malic enzyme, withdrawing oxaloacetate back to the glycolysis and therefore detrimental for lysine production, was increased. The reason for this might be a contribution of malic enzyme to NADPH supply in the mutant in the mutant. The observed complex changes are remarkable, because they are due to the minimum genetic modification possible, the exchange of only one single nucleotide.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
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• 제19권6호
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• pp.457-463
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• 2007

Various correlations of saturated vapor density in a truncated power series form are tested and compared in this study. Saturated vapor density correlation can be expressed relating logarithmic reduced density to the reduced temperature. Five types of correlation has been investigated using saturated vapor density data for 22 pure substance refrigerants from ASHRAE (American Society of Heating, Reftigerating and Air-Conditioning Engineers, Inc.) property tables and NIST (National Institute of Standards and Technology) Chemistry Webbook. Correlations are fitted to the data points by least squares method. Data points are equally weighted. The best type of correlation among the five types is suggested. The results obtained indicate that the best correlations with 3, 4, and 5 terms yield average AAD's (Average Absolute Deviation) of 0.27%, 0.04%, and 0.02%, respectively, while widely used conventional correlations with 3, 4, and 5 terms yield those of 1.19%, 0.61%, and 0.17%. The suggested type of correlation could reduce the number of terms while improving performance.

• Asian-Australasian Journal of Animal Sciences
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• 제4권4호
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• pp.369-375
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• 1991

A series of in vitro incubation studies with washed rumen bacteria were conducted to determine the influence of incubation time and concentrations of peptides, alanine, ammonia nitrogen and carbohydrate on the rate of peptide disappearance and on bacterial growth. Disappearance rate of tri-alanine (ala3) under various conditions was between 30.6 and $58.2mg\;hr^-$ per gram bacterial dry matter. Ala3 was removed from the incubation medium in an almost linear fashion as incubation time and ala3 concentration was increased. Washed rumen bacteria utilized ala3 faster than di-l-alanine (ala2) at all concentrations. Adding 9mM carbohydrate significantly increased ala3 disappearance, but level of ammonia nitrogen had no influence on ala3 disappearance. The presence of alanine in the medium significantly lowered ala3 utilization by rumen bacteria. Bacterial dry matter and nitrogen growth yield were not influenced by alanine and peptides when incubation medium already contained a sufficient level of ammonia nitrogen. Increased ammonia nitrogen in the presence of ala3 did not stimulate bacterial growth. Carbohydrate significantly increased bacterial dry matter and nitrogen growth as expected. Results indicate that the rate of peptide utilization by rumen bacteria may be altered by type and concentration of peptides, and energy supply, and this may be mediated through changes in numbers and type of bacteria.

• Transactions of the Korean Society of Mechanical Engineers
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• 제18권10호
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• pp.2706-2711
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• 1994

In this work, an experimental type of torque converter has been constructed and its characteristics have been evaluated by adapting an electrorheological fluid(ERF) as an operating medium. The device was designed by using the equations which were proposed by Carlson et al. The correlation between the rheological behaviour of an ERF and mechanical parameters of the clutch has been investigated. The torque generated by an ERF in this device is sum of one due to the yield strength by polarizing dispersed particles in dielectric oil and one due to the viscous drag. The experimental results are presented in terms of torque and current density as a function of rotational speed at various electric field strength applied. Experimental results showed that the measured torque was rapidly increased with the increase of the electric field, generally being proportional to the rotational speed of the motor. The measured current was shown to be increased with the increased electric field. Also, the current was decreased with the increase of increased with the increased electric field. Also, the current was decreased with the increase of the rotational speed of the motor and reached plateau region after f = 5 Hz.

• Journal of Welding and Joining
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• 제28권4호
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• pp.33-40
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• 2010

The purpose of this study is to establish a predictive equation of transverse residual stress at the thick FCA butt weldment of large container vessel. The variables used were restraint degree, yield strength of base material, thickness of weldment and welding heat input. Restraint degree at the thick weldment of container ship having the various welding sequence was calculated using FEA. From the result, the H-type specimen was designed to reproduce the level of restraint degree at the actual weldment of containership. Based on the results, the predictive equations of the mean value and the distribution of transverse residual stress at each location of the weldment were established using dimensional analysis and multiple-regression method. The predictive equations were verified by comparing with those measured by XRD in the actual weldment of the ship.

• Korean Chemical Engineering Research
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• 제52권1호
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• pp.63-67
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• 2014

The effects of reactant composition on the particle size distribution, synthetic yield, and density of Phenol-formaldehyde bead were examined in the synthesis of resol-type phenolic resin. Decrease of the content of DI water as dispersion media can increase the viscosity of suspension, which may cause the difference of particle size distribution and aggregation. The average particle size of synthesized beads was also decreased with the increasing content of stabilizer which can affect the interfacial area. The amount of crosslinking agent showed no effect on the size distribution and synthetic yield, but it made a decrease in the density of synthesized bead due to the macropore in the bead.

• BMB Reports
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• 제44권6호
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• pp.375-380
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• 2011

Bacillus licheniformis SK-1 naturally produces chitinase 72 (CHI72) with two truncation derivatives at the C-terminus, one with deletion of the chitin binding domain (ChBD), and the other with deletions of both fibronectin type III domain (FnIIID) and ChBD. We constructed deletions mutants of CHI72 with deletion of ChBD (CHI72${\Delta}$ChBD) and deletions of both FnIIID and ChBD (CHI72${\Delta}$FnIIID${\Delta}$ChBD), and studied their activity on soluble, amorphous and crystalline substrates. Interestingly, when equivalent amount of specific activity of each enzyme on soluble substrate was used, the product yield from CHI72-${\Delta}$ChBD and CHI72${\Delta}$FnIIID${\Delta}$ChBD on colloidal chitin was 2.5 and 1.6 fold higher than CHI72, respectively. In contrast, the product yield from CHI72${\Delta}$ChBD and CHI72${\Delta}$FnIIID-${\Delta}$ChBD on ${\beta}$-chitin reduced to 0.7 and 0.5 fold of CHI72, respectively. These results suggest that CHI72 can modulate its substrate specificities through truncations of the functional domains at the C-terminus, producing a mixture of enzymes with elevated efficiency of hydrolysis.

• Proceedings of The Korean Society of Agricultural and Forest Meteorology Conference
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• 한국농림기상학회 2011년도 학술발표회
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• pp.16-18
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• 2011

Evaluation of climate change on the rice productivity was conducted using crop growth simulation model, where Odae, Hwaseong, Ilpum were used as a representative cultivar of early, medium, and medium-late rice maturity type, respectively, and climate change scenario 'A1B' was applied to weather data for future climate change at 57sites. When cropping season was fixed, rice yield decreased by 4~35% as climate change which was caused by poor filled grain ratio with high temperature and low irradiation during grain-filling. When cropping season was changed, rice yield decreased by only 0~5% as climate change which was caused poor filled grain ratio with low irradiation during grain-filling period. However, this irradiation decline was less than when cropping season was fixed. Therefore, we need to develop rice cultivars resistant to low irradiation which can maintain high filled grain ratio under poor irradiation condition, and late maturity rice cultivars whose growing period is longer than the present medium-late maturity type.

• Structural Engineering and Mechanics
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• 제84권2호
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• pp.239-251
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• 2022

The use of lateral reinforcement in confined concrete columns can improve bearing capacity and deformability. The lateral responses of lateral reinforcement significantly influence the effective confining pressure on core concrete. However, lateral strain-axial strain model of concrete columns confined by lateral reinforcement has not received enough attention. In this paper, based on experimental results of 85 concrete columns confined by lateral reinforcement under axial compression, the effect of unconfined concrete compressive strength, volumetric ratio, lateral reinforcement yield strength, and confinement type on lateral strain-axial strain curves was investigated. Through parameter analysis, it indicated that with the same level of axial strain, the lateral strain slightly increased with the increase in the unconfined concrete compressive strength, but decreased with the increase in volumetric ratio significantly. The lateral reinforcement yield strength had slight influence on lateral strain-axial strain curves. At the same level of lateral strain, the axial strain of specimen with spiral was larger than that of specimen with stirrup. Furthermore, a lateral strain-axial strain model for concrete columns confined by lateral reinforcement under axial compression was proposed by introducing the effects of unconfined concrete compressive strength, volumetric ratio, confinement type and effective confining pressure, which showed good agreement with the experimental results.